Allele Dmel\ci^{N:Zn.Scer\UAS}

General Information
Symbol	Dmel\ci^{N:Zn.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0057694
Feature type	allele	Associated gene	Dmel\ci

Allele class
Mutagen	in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms neuroanatomy defective ventral multidendritic neuron vdaB
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Hepker et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	ci^Scer\UAS.cHb (Hepker et al., 1997)
Nature of the lesion	Statement Reference Construct: Deletion of C-terminal ci coding region beyond amino acid 684. Expression is governed by Scer\UAS regulatory sequences. (Hepker et al., 1997)
Carried in construct	P{UAS-ciN/Zn} (Merabet et al., 2005, Hayashi and Saigo, 2001, Suzuki and Saigo, 2000, Alexandre et al., 1999, Kato et al., 2004, Hepker et al., 1997, Croker et al., 2006, Karim and Moore, 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^{neur-GAL4-A101} (Karim and Moore, 2011)
Phenotype Manifest In
	Bolwig organ, with Scer\GAL4^da.G32 (Suzuki and Saigo, 2000) denticle belt, with Scer\GAL4^en-e16E (Alexandre et al., 1999) denticle belt, with Scer\GAL4^ptc-559.1 (Hepker et al., 1997) filzkorper, with Scer\GAL4^ems.HRE (Merabet et al., 2005) stigmatophore, with Scer\GAL4^salm-459.2 (Merabet et al., 2005) tracheal tip cell \| embryonic stage, with Scer\GAL4^btl.PS (Kato et al., 2004) ventral multidendritic neuron vdaB, with Scer\GAL4^{neur-GAL4-A101} (Karim and Moore, 2011)
Detailed Description
	Statement Reference Expression of ci[N:Zn.Scer\UAS] under the control of Scer\GAL4[neur-GAL4-A101] results in the loss of the ventral vdaB class IV neuron in 24% of stage 15 parasegments; however, the more lateral v'ada is never lost. Posterior derived MD neurons are also lost. For example, vbd is lost in 75% of parasegments. Examination of stage 12 embryos reveals that vdaB-loss is preceded by SOP1a loss in 22% of cases. SOP4a are not affected. (Karim and Moore, 2011) Expression of ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^ems.HRE impairs filzkorper formation but does not affect stigmatophore formation. However, expression of this transgene under the control of Scer\GAL4^salm-459.2 has the reciprocal effect; stigmatophore formation is impaired while filzkorper formation is unaffected. (Merabet et al., 2005) In ci^{N:Zn.Scer\UAS}; Scer\GAL4^btl.PS embryos, terminal tracheal branches are frequently duplicated. Time-lapse observation shows that the terminal branches initially develop normally, but that secondary terminal branches then form and migrate aberrantly anteriorly. About 1/3 of these duplicated branches arise from a single terminal cells. (Kato et al., 2004) Bolwig's organ formation is suppressed in embryos expressing ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^da.G32. Bolwig's organs are formed in embryos expressing ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^h-1J3. (Suzuki and Saigo, 2000) Embryos expressing ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^en-e16E have an abnormal denticle pattern. In the middle of each belt, the denticle rows show a mirror-image 5, 4, 4, 5 pattern (numbers indicate the wild-type denticle row that the altered denticle rows resemble). This pattern is flanked by small denticles pointing away from the belt. The small anterior-pointing denticles at the anterior of each belt could either be type 1 or 6 by morphology. (Alexandre et al., 1999) When expression is driven by Scer\GAL4^ptc-559.1 in the embryo, naked cuticle is deleted and the denticle belts are partially duplicated. (Hepker et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^da.G32, ci^{N:Zn.Scer\UAS} has Bolwig organ phenotype, suppressible \| partially by hh^Scer\UAS.cKa/Scer\GAL4^da.G32 (Suzuki and Saigo, 2000)
Additional Comments
Genetic Interactions
Statement Reference The Bolwig's organ phenotype in embryos expressing ci^{N:Zn.Scer\UAS} under the control of Scer\GAL4^da.G32 is considerably rescued by co-expression of hh^Scer\UAS.cKa. (Suzuki and Saigo, 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	ci^{N:Zn.Scer\UAS} ci^{N/Zn.Scer\UAS} ci^N/Zn.UAS
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Karim and Moore, 2011, J. Neurosci. 31(47): 17017--17027 Convergent local identity and topographic projection of sensory neurons. [FBrf0216770] Croker et al., 2006, Dev. Biol. 291(2): 368--381 Regulation of the Drosophila transcription factor, Cubitus interruptus, by two conserved domains. [FBrf0190106] Merabet et al., 2005, Development 132(13): 3093--3102 Hox-controlled reorganisation of intrasegmental patterning cues underlies Drosophila posterior spiracle organogenesis. [FBrf0187474] Kato et al., 2004, Development 131(21): 5253--5261 Hedgehog and Decapentaplegic instruct polarized growth of cell extensions in the Drosophila trachea. [FBrf0180164] Hayashi and Saigo, 2001, Mech. Dev. 108(1-2): 13--27 Diversification of cell types in the Drosophila eye by differential expression of prepattern genes. [FBrf0139741] Suzuki and Saigo, 2000, Development 127(7): 1531--1540 Transcriptional regulation of atonal required for Drosophila larval eye development by concerted action of eyes absent, sine oculis and hedgehog signaling independent of fused kinase and cubitus interruptus. [FBrf0126773] Alexandre et al., 1999, Development 126(24): 5689--5698 Wingless and Hedgehog pattern Drosophila denticle belts by regulating the production of short-range signals. [FBrf0111771] Hepker et al., 1997, Development 124(2): 549--558 Drosophila cubitus interruptus forms a negative feedback loop with patched and regulates expression of Hedgehog target genes. [FBrf0092552]

Allele Dmel\ciN:Zn.Scer\UAS

Allele Dmel\ci^{N:Zn.Scer\UAS}