|Name||Saccharomyces cerevisiae UAS construct a of Sutherland||FlyBase ID||FBal0057743|
|Feature type||allele||Associated gene||Dmel\bnl|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Stahl et al., 2007, Myat et al., 2005, Shim et al., 2001, Ohshiro et al., 2002, Hemphala et al., 2003, Dorfman et al., 2002, Ribeiro et al., 2002, Ahmad and Baker, 2002, Sato and Kornberg, 2002, Nussbaumer et al., 2000, Wolf and Schuh, 2000, Steneberg et al., 1999, Jarecki et al., 1999, Lin et al., 1999, Michelson et al., 1998, Englund et al., 1999, Imam et al., 1999, Gabay et al., 1997, Shilo et al., 1997, Hacohen et al., 1998, Sutherland et al., 1996, Ikeya and Hayashi, 1999, Kadam et al., 2009, Wolf et al., 2002, Araújo and Casanova, 2011)
|Phenotype Manifest In|
In the CNS, bnl[Scer\UAS.cSa] expression in the midline using Scer\GAL4[sim.PS] results in the abrogation of ganglionic branching. Higher levels of expression, under the control of Scer\GAL4 cause a more extreme phenotype, with many cells adopting terminal fate.
Expression of bnl[Scer\UAS.cSa] under the control of Scer\GAL4[69B] has little or no effect on mesoderm monolayer formation in the embryo following gastrulation.
When bnlScer\UAS.cSa is driven by Scer\GAL4bap.3 about a 1/4 of visceral mesoderm parasegments, visceral branches bifurcate, in about 2.4% they show simple misrouting. These defects persist until at least stage 15.
Ectopic expression of bnlScer\UAS.cSa under the control of Scer\GAL4Act5C.PP in clones in the male genital disc results in the migration of btl-expressing cells into a nearby ectopic domain where they are not observed in wild-type discs.
Tracheal cells of all types in bnlScer\UAS.cSa; Scer\GAL4btl.PS embryos have numerous ectopic filpodia.
In late third instar bnlScer\UAS.cSa; Scer\GAL4dpp.blk1 larvae ectopic tracheoblasts can be seen adhering to the disc in the vicinity of the dpp expression domain. When somatic clones of bnlScer\UAS.cSa; Scer\GAL4Act5C.PI cells are randomly induced in wing discs, ectopic tracheoblasts migrate along the surface of disc towards these clones.
Ectopic cellular extensions are seen in the tracheal cells of stage 12 embryos expressing bnl[Scer\UAS.cSa] under the control of Scer\GAL4[btl.PS].
Embryos expressing bnlScer\UAS.cSa under the control of Scer\GAL4btl.PS develop complete dorsal trunk structures but lack the other primary branches.
Branching of the dorsal branch and lateral trunk of the tracheal system is suppressed in embryos expressing bnlScer\UAS.cSa under the control of Scer\GAL4btl.PS and the dorsal trunk appears thickened.
Ubiquitous expression of bnlScer\UAS.cSa driven by Scer\GAL4hs.PB and heatshocked for 1 hour during the first larval instar, causes a dramatic proliferation of terminal branches. However, unlike normal terminal branches, which are distributed evenly over their targets, these remain clumped together in a large tangled mass near the point of origin. Over expression of bnlScer\UAS.cSa when driven by Scer\GAL4A9 in the CNS, transforms the normal ladder-like pattern of GB tracheal branches into a massive tangle of branches that completely cover the CNS. When driven by Scer\GAL4hs.PB, a similar effect is seen in the LG trachea. A similar effect is seen when expressed in most tissues, but the heart and imaginal discs, which normally express bnl but lack tracheal branches, fail to attract branches even when the gene is overexpressed there. When bnlScer\UAS.cSa is driven in the salivary glands (which do not normally express bnl or receive trachea) by Scer\GAL471B during larval development, terminal tracheal branches are attracted to the salivary glands from nearby tissues. When bnlScer\UAS.cSa is driven in muscle fibre 12 by Scer\GAL45053A the LG terminal branches are directed towards this muscle, away from their normal targets at the ventral midline, doubling the number seen terminating on this muscle (as compared to wild-type) when raised at 25oC and quadrupling the number when raised at 29oC. Expression of bnlScer\UAS.cSa in random single cells (Driven by Scer\GAL4Act5C.PP in a Scer\FLP1hs.PS induced clones.), causes a local proliferation of terminal branches that grow out to the expressing cells arborizing on the cells and almost completely engulfing them. Attraction occurs over distances of up to about 10 cell diameters. Cases can be found where a terminal branch bifurcates to target two bnlScer\UAS.cSa expressing cells separated by several cell diameters.
Expression of bnlScer\UAS.cSa under the control of Scer\GAL469B results in marked defects in tracheal branching; primary branching is suppressed and an overabundance of secondary and terminal branches is induced.
When expression is driven at stage 11 by heat induced Scer\GAL4hs.PB the number of cells expression pantip and terminal markers is increased in all tracheal branches. Extra terminal branching occurs.
Expression of bnlScer\UAS.cSa under the control of Scer\GAL469B results in a reduction of primary branching and an increase in the formation of fine secondary and terminal branches in the embryonic tracheal system.
|Phenotype Manifest In|
Scer\GAL469B, bnlScer\UAS.cSa has embryonic/larval tracheal system phenotype, suppressible | partially by sfl03844
Scer\GAL469B, bnlScer\UAS.cSa has embryonic/larval tracheal system phenotype, suppressible | partially by sgl08310
|NOT suppressed by|
bnlScer\UAS.cSa/Scer\GAL469B is a suppressor | partially of embryonic/larval tracheal system phenotype of sfl03844
bnlScer\UAS.cSa/Scer\GAL469B is a suppressor | partially of embryonic/larval tracheal system phenotype of sgl08310
Simultaneous expression of seq[Scer\UAS.cBa] and bnl[Scer\UAS.cSa] in the CNS under the control of Scer\GAL4 has the same effect as over-expression of bnl[Scer\UAS.cSa] alone.
When srScer\UAS.cBa and bnlScer\UAS.cSa are both driven by Scer\GAL4sim.PS a dramatic tracheal sprouting is seen in the salivary glands, showing an enhancement of the bnlScer\UAS.cSa, Scer\GAL4sim.PS phenotype.
hb15 mutant embryos that express bnlScer\UAS.cSa ectopically have no signs of dorsal trunk outgrowth at all.
When expression is driven in the mesoderm of a stumps09904b mutant by Scer\GAL4twi.PG, the portions of the tracheal sacs near the bnlScer\UAS.cSa expressing cells significant cytoplasmic outgrowth and ramification of terminal branches. Some erratic of primary branches also occurs. These features are not normally seen in stumps09904b mutants. Similar effects are seen when bnlScer\UAS.cSa is expressed under the control of Scer\GAL4twi.PG in Df(3R)γ2 homozygotes.
The tracheal branching defects caused by expression of bnlScer\UAS.cSa under the control of Scer\GAL469B are weakly blocked if the embryos are homozygous for sfl03844 or sgl08310. Partially restores tracheal branching in sfl03844 embryos derived from sfl03844 female germline clones (lacking both maternal and zygotic sfl function), when expressed under the control of Scer\GAL469B. Partially restores tracheal branching in sgl08310 embryos derived from sgl08310 female germline clones (lacking both maternal and zygotic sgl function), when expressed under the control of Scer\GAL469B.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Sutherland
|Secondary FlyBase IDs|
|References ( 26 )|
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|Recent research papers ( 1 )|