Allele Dmel\barr^L305

General Information
Symbol	Dmel\barr^L305	Species	D. melanogaster
Name		FlyBase ID	FBal0057771
Feature type	allele	Associated gene	Dmel\barr

Allele class	amorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Bhat et al., 1996)
Mutagen	P-element activity (Bhat et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	barr^k00310 (Bhat et al., 1996)
Nature of the lesion	Statement Reference Imprecise excision of P{lacW} insertion. (Bhat et al., 1996)
Caused by insertion	P{?'lacW}barr^L305 (Bhat et al., 1996, Lupo et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	enhancer of variegation, with In(1)w^m4h (Cobbe et al., 2006) enhancer of variegation \| partially, with P{hsp26-pt-T}39C-72 (Cobbe et al., 2006) lethal \| embryonic stage \| recessive (Bhat et al., 1996) non-enhancer of variegation, with P{hsp26-pt-T}118E-10 (Cobbe et al., 2006) suppressor of variegation \| dominant, with In(1)w^m4h (Dej et al., 2004)
Phenotype Manifest In
	neuron (Bhat et al., 1996) peripheral nervous system (Bhat et al., 1996)
Detailed Description
	Statement Reference Average Citrate Synthase activity is increased by 4% in heterozygous males (Chen et al., 2008) Homozygous embryos exhibit severe loss of PNS neurons and a significant increase in size of remaining neurons. Embryos arrest in cycle 16, in the majority of anaphase figures chromatids do not resolve but remain interlocked to form extensive chromatin bridges. The unresolved chromatids decondense without segregation and cytokinesis is attempted. Trl staining reveals mutation does not prevent centromere separation. (Bhat et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement Reference barr^L305 has enhancer of variegation phenotype, non-suppressible by glu^88-82 (Cobbe et al., 2006)
Suppressor of
Statement Reference barr[+]/barr^L305 is a suppressor of visible phenotype of w^Fab-7.5F24 (Lupo et al., 2001)
NOT Suppressor of
Statement Reference barr^L305 is a non-suppressor of suppressor of variegation phenotype of In(1)w^m4, glu^88-82 (Cobbe et al., 2006)
Phenotype Manifest In
NOT Enhanced by
Statement Reference barr^L305 has abdominal segment 6 phenotype, non-enhanceable by Abd-B^Fab7-1/Abd-B[+] (Lupo et al., 2001)
NOT suppressed by
Statement Reference barr^L305 has abdominal segment 6 phenotype, non-suppressible by Abd-B^Fab7-1/Abd-B[+] (Lupo et al., 2001)
Enhancer of
Statement Reference barr[+]/barr^L305 is an enhancer of abdominal segment 6 phenotype of Abd-B^Fab7-2 (Lupo et al., 2001)
NOT Enhancer of
Statement Reference barr[+]/barr^L305 is a non-enhancer of abdominal segment 6 phenotype of Abd-B^Fab7-1 (Lupo et al., 2001)
Suppressor of
Statement Reference barr[+]/barr^L305 is a suppressor of pigment cell phenotype of w^Fab-7.5F24 (Lupo et al., 2001)
NOT Suppressor of
Statement Reference barr[+]/barr^L305 is a non-suppressor of abdominal segment 6 phenotype of Abd-B^Fab7-1 (Lupo et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Intermediate eye pigment levels are observed in glu^88-82, barr^L305 double mutants with a In(1)w^m4h background, showing that glu^88-82 suppressor effects of PEV are not dominant over the enhancer activity of barr^L305. (Cobbe et al., 2006) Chromatin condenses nonuniformly in prophase and prometaphase in postblastoderm Cap-G^K1 barr^L305 double mutant embryos. By metaphase, chromosome condensation has reached wild-type levels. Chromatid separation defects are seen in anaphase when only the fourth chromosome is clearly seen to separate, and lagging chromosomes are seen in telophase. The double mutant postblastoderm embryos have an increased number of prometaphase figures compared to wild-type embryos, suggesting that the length of prometaphase may be increased in the mutant embryos. (Dej et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	barr^L305 is rescued by barr^hs.PB (Bhat et al., 1996)
Comments	barr^hs.PB rescues the lethality of barr^L305 mutation. (Bhat et al., 1996)
Stocks ( 2 )
Bloomington	4402 y¹ w^67c23; barr^L305/CyO
Kyoto	107718 y¹ w^67c23; barr^L305/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	barr^L305 (Christensen and Cook, 2007.3.22, Cook et al., 2009.11.25, Chen et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Chen et al., 2008, Genome Res. 18(1): 123--136 Identification of novel modulators of mitochondrial function by a genome-wide RNAi screen in Drosophila melanogaster. [FBrf0202125] Cobbe et al., 2006, Genetics 172(2): 991--1008 Diverse mitotic and interphase functions of condensins in Drosophila. [FBrf0190711] Dej et al., 2004, Genetics 168(2): 895--906 Mutations in the Drosophila condensin subunit dCAP-G: defining the role of condensin for chromosome condensation in mitosis and gene expression in interphase. [FBrf0180304] Lupo et al., 2001, Mol. Cell 7(1): 127--136 Drosophila chromosome condensation proteins Topoisomerase II and Barren colocalize with Polycomb and maintain Fab-7 PRE silencing. [FBrf0134762] Bhat et al., 1996, Cell 87(6): 1103--1114 Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with Topoisomerase II. [FBrf0091018]
Personal communication to FlyBase	Cook et al., 2009.11.25, Isolation and characterization of Df(2L)BSC860. Isolation and characterization of Df(2L)BSC860. [FBrf0209486] Christensen and Cook, 2007.3.22, Isolation and characterization of Df(2L)BSC258. Isolation and characterization of Df(2L)BSC258. [FBrf0199245]

Allele Dmel\barrL305

Allele Dmel\barr^L305