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General Information
Symbol
Dmel\armS14.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0057837
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
armS14
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A mutated form of arm, lacking the end of the N-terminal region (amino acids 101-139 are deleted), is expressed under the control of its own promoter. The protein is tagged with a Tag:MYC epitope at residue 784 in the Gly/Pro-rich region close to the C-terminus. arm 3' trailer sequences are also present. The protein produced shows slightly reduced levels of binding to shg protein compared to wild-type controls in a coimmunoprecipitation assay and binding to α-Cat protein is abolished.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryos expressing armS14.T:Hsap\MYC in an arm1 mutant background do not show a wg loss of function phenotype in the cuticle. The embryos have defects in the ventral epidermis and the head that resemble a weak to moderate shg mutant phenotype. In particular, there are foci of cell death in and around the optic placodes. The optic placode does form but loses its epithelial structure and fails to invaginate in late embryos.

The size of the primary branches of the tracheal system is largely unaffected in embryos carrying armS14.T:Hsap\MYC in an arm4 background, even though segments of the dorsal trunk are disconnected.

Photoreceptors are not rescued from death in ApcQ8 homozygotes carrying one copy of armS14.T:Hsap\MYC in an arm4/+ background.

Embryos do not exhibit segment polarity defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

The phenotype seen in RP2 neurons in embryos that are zygotically arm4 but have wild-type maternal arm is rescued by armS14.T:Hsap\MYC.

Partial rescue of adherens junction defect.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Used in a coimmunoprecipitation assay to study shg binding and analysed by SDS-PAGE to study protein phosphorylation and stability.

Mutation abolished the ability of arm to bind α-Cat in vivo.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
armS14.T:Hsap\MYC
armS14.T:Myc
armS14.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (8)