Allele Dmel\arm^{S12.T:Hsap\MYC}

General Information
Symbol	Dmel\arm^{S12.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0057838
Feature type	allele	Associated gene	Dmel\arm
Also Known As	Arm^S12

Allele class
Mutagen	in vitro construct - site directed mutagenesis, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Orsulic and Peifer, 1996, Ahmed et al., 1998) in vitro construct - site directed mutagenesis (Orsulic and Peifer, 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	arm^{S2.T:Hsap\MYC} (Orsulic and Peifer, 1996)
Nature of the lesion	Statement Reference Alteration of repeat 1. (van de Wetering et al., 1997) Construct: Amino acids 549-606 are deleted (fusion of repeats 10 and 11 and deletion of the insertion between these repeats). (Orsulic and Peifer, 1996)
Carried in construct	P{arm.S12} (Thompson, 2004, Ahmed et al., 1998, Orsulic and Peifer, 1996)
Tagged with	T:Hsap\MYC (Ahmed et al., 1998, Thompson, 2004, Orsulic and Peifer, 1996, van de Wetering et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type (Orsulic and Peifer, 1996)
Phenotype Manifest In
	denticle \| ectopic, with arm⁴ (Thompson, 2004) embryo \| segment polarity expression pattern (Orsulic and Peifer, 1996) embryonic/first instar larval cuticle, with arm⁴ (Thompson, 2004) segment (Orsulic and Peifer, 1996)
Detailed Description
	Statement Reference Embryos expressing arm^{S12.T:Hsap\MYC} in a arm⁴ background have a "lawn of denticles" cuticle phenotype. (Thompson, 2004) Many photoreceptors are rescued from death in Apc^Q8 homozygotes carrying one copy of arm^{S12.T:Hsap\MYC} in an arm⁴/+ background. (Ahmed et al., 1998) Embryos exhibit very weak segment polarity defects, adherens junctions are normal. (Orsulic and Peifer, 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference arm⁴, arm^{S12.T:Hsap\MYC} has denticle \| ectopic phenotype, suppressible \| partially by pan::pygo^{pygoΔPHD-ΔNTCF.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PS (Thompson, 2004) arm⁴, arm^{S12.T:Hsap\MYC} has embryonic/first instar larval cuticle phenotype, suppressible \| partially by pan::pygo^{pygoΔPHD-ΔNTCF.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PS (Thompson, 2004)
Additional Comments
Genetic Interactions
Statement Reference The "lawn of denticles" cuticle phenotype seen in embryos carrying arm^{S12.T:Hsap\MYC} in an arm⁴ background is substantially rescued by expression of pan::pygo^{pygoΔPHD-ΔNTCF.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS; a segmented pattern of denticle bands and naked cuticle is restored. (Thompson, 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Not rescued by	arm⁴/arm^{S12.T:Hsap\MYC} is not rescued by Scer\GAL4^arm.PS/arm^{Scer\UAS.T:TM-sev} (Thompson, 2004)
Rescues	arm^{S12.T:Hsap\MYC} rescues arm³ (Orsulic and Peifer, 1996)
Comments	The "lawn of denticles" cuticle phenotype seen in embryos carrying arm^{S12.T:Hsap\MYC} in an arm⁴ background is not rescued by ubiquitous expression of arm^{Scer\UAS.T:sev-TM} using Scer\GAL4^arm.PS. Ubiquitous expression of arm^{S10.Scer\UAS.T:Hsap\MYC} using Scer\GAL4^arm.PS produces ectopic naked cuticle in embryos carrying arm^{S12.T:Hsap\MYC} in an arm⁴ background. (Thompson, 2004)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Used in a coimmunoprecipitation assay to study shg binding and analysed by SDS-PAGE to study protein phosphorylation and stability. (Orsulic and Peifer, 1996)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	arm^{S12.T:Hsap\MYC} arm^S12.T:Myc Arm^S12 (Thompson, 2004) arm^S12 (van de Wetering et al., 1997) S12 (Ahmed et al., 1998)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Thompson, 2004, Curr. Biol. 14(6): 458--466 A complex of Armadillo, Legless, and Pygopus coactivates dTCF to activate wingless target genes. [FBrf0174483] Ahmed et al., 1998, Cell 93(7): 1171--1182 Regulation of armadillo by a Drosophila APC inhibits neuronal apoptosis during retinal development. [FBrf0102882] van de Wetering et al., 1997, Cell 88(6): 789--799 Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF. [FBrf0093264] Orsulic and Peifer, 1996, J. Cell Biol. 134(5): 1283--1300 An in vivo structure-function study of armadillo, the beta-catenin homologue, reveals both separate and overlapping regions of the protein required for cell adhesion and for wingless signaling. [FBrf0090729]

Allele Dmel\armS12.T:Hsap\MYC

Allele Dmel\arm^{S12.T:Hsap\MYC}