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General Information
Symbol
Dmel\armΔN.UAS.Tag:HA,Tag:Myr(Unk)
Species
D. melanogaster
Name
FlyBase ID
FBal0057864
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ΔArm, UAS-fluΔarm, UAS-Flu-ΔArm, UAS-arm*
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of a constitutively active form of arm that lacks the N-terminal part of the protein (amino acids 155-843 are present). The protein is tagged at the N-terminal end with a myristoylation signal (MGNKCCSKRQGTMAGNI) followed by two copies of the Tag:HA epitope. Sequence encoding an inactive nuclear localization signal has been added at the C-terminal end of the protein.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Flip-out clones in the salivary gland expressing armDelta.Scer\UAS.T:Ivir\HA1 have abonormally small nuclei.
The wings of armΔ.Scer\UAS.T:Ivir\HA1; Scer\GAL4vg.PM flies have tufts of ectopic wing margin bristles.
Expression of armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PY results in a slight increase in apoptosis in third larval instar eye discs compared to controls. Expression of armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PY results in a rough eye phenotype.
Somatic clones of armΔ.Scer\UAS.T:Ivir\HA1; Scer\GAL4αTub84B.PZ cells in the periphery (proximal regions) of the wing disc show increased BrdU incorporation within the clone compared to wild-type. These clones also distort the morphology of the late third instar wing disc.
When armΔ.Scer\UAS.T:Ivir\HA1 is driven by Scer\GAL4arm.PS a naked cuticle phenotype is seen. Co-expression of armT:Hsap\MYC,T:Hsap\CAAX and armΔ.Scer\UAS.T:Ivir\HA1 (driven by Scer\GAL4arm.PS) in either a wild-type background, or in a arm2 background, leads to embryos with naked cuticle. When armT:Hsap\MYC,T:Hsap\CAAX and armΔ.Scer\UAS.T:Ivir\HA1 are expressed in a arm1 background however, ectopic denticles are seen. When armΔ.Scer\UAS.T:Ivir\HA1 is driven in a armF1α germ-line clone background, a cuticle pattern with periodic denticle belts and regions of intervening naked cuticle are formed.
Overexpression of armΔ.Scer\UAS.T:Ivir\HA1, under the regulation of Scer\GAL4da.G32 produces a largely naked ventral cuticle.
armΔ.Scer\UAS.T:Ivir\HA1; Scer\GAL471B flies have severely abnormal wings.
Clones in the wing pouch region of the wing disc expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP during the third larval instar have many fewer cells than control clones induced in parallel. This is primarily due to a slower rate of cell division. Cells in the wing disc expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP cycle aberrantly, compared to internal control cells; the cells have cell cycle phasing characteristic of mature wing disc cells, rather than phasing appropriate for their chronological age. Cell size is normal.
Clones in the eye expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI show considerable overgrowth and do not differentiate as ommatidia.
Internal ventral clones in the eye expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI can suppress eye development, whereas clones at the posterior margin do not suppress eye fate.
Clones in the genital disc expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP tend to sort out from the surrounding epithelium.
Expression of armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PS results in the complete absence of denticles from the cuticle. Embryos derived from females with arm2 homozygous germline clones and carrying Scer\GAL4arm.PS crossed to males carrying armΔ.Scer\UAS.T:Ivir\HA1 produce embryos with 4 different cuticle phenotypes; small abnormally shaped, denticle covered cuticles (25%, characteristic of arm2), naked cuticles (25%, characteristic of activated arm), wild-type cuticles (25%) and a phenotype where the embryo shows the cell transformations to naked cuticle, but is small and abnormally shaped (25%). This last phenotype seems to be intermediate between the naked and arm2 phenotype.
Clones expressing armΔ.Scer\UAS.T:Ivir\HA1 in the eye disc under the control of Scer\GAL4Act5C.PP do not show photoreceptor differentiation within the clone.
Embryos expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GQ2 have a naked cuticle phenotype. Adults expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GF1 have abdominal sternites which are enlarged at the expense of the adjacent pleural membrane. Adults expressing armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4HV1 have extra wing vein material between wing veins 2 and 3.
Causes a 'naked' phenotype, in which the denticles normally located in the anterior of each embryonic segment are partially or completely replaced with naked cuticle, when expressed in a wild-type or ciCe-1 background using Scer\GAL4hs.PB. Does not suppress the lawn of denticles phenotype of pan13 flies when expressed using Scer\GAL4hs.PB, indicating that pan acts downstream of arm.
Expression under the control of Scer\GAL4hs.PB causes a larval naked phenotype.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Ectopic wing margin bristles on the wings of armΔ.Scer\UAS.T:Ivir\HA1; Scer\GAL4vg.PM animals are unaffected by twsj11C8/+.
Co-expression of CbydsRNA.Scer\UAS.WIZ in a armF1α, armΔ.Scer\UAS.T:Ivir\HA1, Scer\GAL4arm.PS background leads to increased naked cuticle compared to armF1α, armΔ.Scer\UAS.T:Ivir\HA1, Scer\GAL4arm.PS embryos.
armF1α, armΔ.Scer\UAS.T:Ivir\HA1, CbydsRNA.Scer\UAS.WIZ (driven by Scer\GAL4arm.PS) germ-line clones exhibit a naked cuticle phenotype. When CbydsRNA.Scer\UAS.WIZ is driven by Scer\GAL4arm.PS in a armΔ.Scer\UAS.T:Ivir\HA1 germ-line clone background, the ectopic denticle phenotype is enhanced.
Expression of armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32 has no effect on the cuticle phenotype of pygoS28 or pygoS123 mutant embryos which lack both maternal and zygotic pygo function.
The wing phenotype of armΔ.Scer\UAS.T:Ivir\HA1; Scer\GAL471B flies is almost completely suppressed by nmoc5-1.Scer\UAS.
Coexpression of both armΔ.Scer\UAS.T:Ivir\HA1 and tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI suppresses eye development in both the dorsal and ventral domains and both in marginal and internal regions of the eye disc. Some of these clones are associated with tissue overgrowth in the adult eye. Coexpression of both armΔ.Scer\UAS.T:Ivir\HA1 and tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 results in complete suppression of the eye. Coexpression of both armΔ.Scer\UAS.T:Ivir\HA1 and tshScer\UAS.cGa under the control of Scer\GAL4bi-omb-Gal4 results in eyes which are reduced in both the dorsal and ventral margins in discs and in adults. Coexpression of both wgScer\UAS.cAa and tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI results in eye suppression in the dorsal eye disc. Coexpression of both wgScer\UAS.cAa and tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 results in suppression of eye fate at the dorsal and ventral margins of the eye.
Co-expression of armΔ.Scer\UAS.T:Ivir\HA1 and tkvQ253D.Scer\UAS.cNb under the control of Scer\GAL4ey.PH in the eye disc often results in ectopic morphogenetic furrow formation from the anterior margin of the disc and a reduction in posterior differentiation.
The naked cuticle phenotype caused by expression of armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4unspecified is partially suppressed by coexpression of AxnA2.Scer\UAS.
The phenotype caused by expression of armΔ.Scer\UAS.T:Ivir\HA1 in clones in the eye disc under the control of Scer\GAL4Act5C.PP is not rescued if the clones are also co-expressing tkvQ253D.Scer\UAS.cNb. Clones expressing Ras85DV12.Scer\UAS in the eye disc under the control of Scer\GAL4Act5C.PP show ectopic photoreceptor formation anterior or posterior to the morphogenetic furrow. This ectopic photoreceptor formation is not prevented by coexpression of armΔ.Scer\UAS.T:Ivir\HA1.
Xenogenetic Interactions
Statement
Reference
Co-expression of armΔ.Scer\UAS.T:Ivir\HA1 and Hsap\ESR1Scer\UAS.cKa under the control of Scer\GAL4GMR.PY results in a marked increase in apoptosis in third larval instar eye discs compared to controls. Treatment with estradiol significantly increases the rate of apoptosis in these double mutant flies. Co-expression of armΔ.Scer\UAS.T:Ivir\HA1 and Hsap\ESR1Scer\UAS.cKa under the control of Scer\GAL4GMR.PY synergistically enhances the rough eye phenotype seen when either is expressed alone. Treatment of the double mutant flies with estrogen further aggravates the severity of the eye phenotype.
Complementation and Rescue Data
Comments
The adhesion defect of arm043A01 is not rescued by expression of armΔ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PS.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Exists in clones, not reported as being germ-line transmitted.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
armΔ.Scer\UAS.T:Ivir\HA1
armΔ.UAS.T:HA1
armΔN.Scer\UAS.T:Ivir\HA1,T:Uuuu\Myr-Unk
armΔN.Scer\UAS.T:Ivir\HA1,T:Uuuu\Myr5
armΔN.UAS.Tag:HA,Tag:Myr(Unk)
Name Synonyms
Secondary FlyBase IDs
    References (32)