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General Information
Symbol
Dmel\Rca12
Species
D. melanogaster
Name
FlyBase ID
FBal0058351
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Rca12 mutant animals fail to execute mitosis after the 16th cell cycle, and subsequently embryos have fewer cells compared to wild-type.

    Rca12 mutant clones generated in the wing imaginal disc have a severe proliferation disadvantage, and are much reduced in size compared to wild-type clones. Mutant cells undergo endoreplication of the DNA, and have an increased nuclear size.

    Rca12/Rca13 embryos exhibit a failure of cell division in the epidermis at mitotic cycle 16: No mitotic spindles are seen at the time when wild-type cells would normally undergo this division, and the DNA is decondensed, suggesting arrest in interphase. Rca12 homozygotes also exhibit cycle 16 cell cycle arrest. Somatic clones of Rca12 in the wing disc are smaller than their corresponding wild-type twinspots. The presence of twinspots in the absence of a corresponding Rca12 mutant clone suggests that mutant cells are sometimes eliminated from the disc.

    Rca12/Rca13 transheterozygous embryos arrest in cell cycle 16.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Suppressor of
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    CycAScer\UAS.T:Ivir\HA1; Scer\GAL4prd.RG1 suppresses the mitotic block due to Rca12/Rca12, in alternate segments. CycEScer\UAS.cLa; Scer\GAL4prd.RG1 suppresses the mitotic block due to Rca12/Rca12, in alternate segments. The embryonic cycle 16 mitotic block due to Rca12/Rca12 is suppressed by Df(1)bi-D3/Df(1)bi-D3. Cell cycle arrest in CycAC8LR1/CycAC8LR1; Rca12/Rca12 embryos, occurs earlier (cycle 15) than in either single mutant (both arrest at cycle 16).

    Xenogenetic Interactions
    Statement
    Reference

    One copy of Rca12 suppresses the increased mitotic index seen in pupal wing clones expressing Hsp83NIG.1242R under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C (co-expressed with Dcr-2Scer\UAS.cDa and BacA\p35Scer\UAS.cHa).

    Complementation and Rescue Data
    Comments

    Expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG, Rca1Δ133.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG, Rca1Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG or Rca1ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4prd.RG1 in a Rca12 mutant background restores embryonic cell numbers to wild-type levels in Scer\GAL4prd.RG1-expressing segments.

    Expression of Rca1Δ255.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG or Rca1C351S.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4prd.RG1 in a Rca12 mutant background fails to restore embryonic cell numbers to wild-type levels in Scer\GAL4prd.RG1-expressing segments.

    Expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4tub in Rca12 mutant wing disc clones suppresses the proliferation disadvantage observed in Rca12 mutant tissue, and clones are similar in size to wild-type. No endoreplication is observed in these cells and nuclear size is comparable to wild-type cells.

    Expression of Rca1Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG or Rca1ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4tub in Rca12 mutant wing disc clones partially suppresses the proliferation disadvantage observed in Rca12 mutant tissue. Clones are increased in size compared to Rca12 mutant clones, but are only a third of the size of wild-type clones. Rarely, endoreplication is observed in mutant clones.

    (with Rca13) Partially rescues: { Rca1<up>Scer\UAS. T:Ivir \HA1</up>, Scer\GAL4prd.RG1 } In Rca12/Rca13; Rca1Scer\UAS.T:Ivir\HA1/Scer\GAL4prd.RG1 embryos, mitosis 16 is rescued in alternating segments resulting in almost twice as many cells in mutant segments as in rescued ones.

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    Mutant
    Wild-type
    Stocks (1)
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    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (5)