Allele Dmel\Rca1²

General Information
Symbol	Dmel\Rca1²	Species	D. melanogaster
Name		FlyBase ID	FBal0058351
Feature type	allele	Associated gene	Dmel\Rca1

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number \| embryonic cycle 16 (Zielke et al., 2006) mitotic cell cycle defective (Dong et al., 1997, Grosskortenhaus and Sprenger, 2002) mitotic cell cycle defective (with Rca1³) (Grosskortenhaus and Sprenger, 2002) mitotic cell cycle defective \| embryonic cycle 16 (Zielke et al., 2006) mitotic cell cycle defective \| somatic clone (Grosskortenhaus and Sprenger, 2002, Zielke et al., 2006)
Phenotype Manifest In
	nucleus \| somatic clone (Zielke et al., 2006) wing disc \| somatic clone (Zielke et al., 2006)
Detailed Description
	Statement Reference Rca1[2] mutant animals fail to execute mitosis after the 16th cell cycle, and subsequently embryos have fewer cells compared to wild-type. Rca1[2] mutant clones generated in the wing imaginal disc have a severe proliferation disadvantage, and are much reduced in size compared to wild-type clones. Mutant cells undergo endoreplication of the DNA, and have an increased nuclear size. (Zielke et al., 2006) Rca1²/Rca1³ embryos exhibit a failure of cell division in the epidermis at mitotic cycle 16: No mitotic spindles are seen at the time when wild-type cells would normally undergo this division, and the DNA is decondensed, suggesting arrest in interphase. Rca1² homozygotes also exhibit cycle 16 cell cycle arrest. Somatic clones of Rca1² in the wing disc are smaller than their corresponding wild-type twinspots. The presence of twinspots in the absence of a corresponding Rca1² mutant clone suggests that mutant cells are sometimes eliminated from the disc. (Grosskortenhaus and Sprenger, 2002) Rca1²/Rca1³ transheterozygous embryos arrest in cell cycle 16. (Dong et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Rca1² has mitotic cell cycle defective phenotype, enhanceable by CycA^C8LR1/CycA^C8LR1 (Grosskortenhaus and Sprenger, 2002)
Suppressed by
Statement Reference Rca1² has mitotic cell cycle defective phenotype, suppressible \| partially by Scer\GAL4^prd.RG1/CycA^{Scer\UAS.T:Ivir\HA1} (Grosskortenhaus and Sprenger, 2002) Rca1² has mitotic cell cycle defective phenotype, suppressible \| partially by Scer\GAL4^prd.RG1/CycE^Scer\UAS.cLa (Grosskortenhaus and Sprenger, 2002) Rca1² has mitotic cell cycle defective phenotype, suppressible by Df(1)bi-D3/Df(1)bi-D3 (Grosskortenhaus and Sprenger, 2002)
Enhancer of
Statement Reference Rca1²/Rca1² is an enhancer of mitotic cell cycle defective phenotype of CycA^C8LR1 (Grosskortenhaus and Sprenger, 2002, Grosskortenhaus and Sprenger, 2002)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference CycA^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^prd.RG1 suppresses the mitotic block due to Rca1²/Rca1², in alternate segments. CycE^Scer\UAS.cLa; Scer\GAL4^prd.RG1 suppresses the mitotic block due to Rca1²/Rca1², in alternate segments. The embryonic cycle 16 mitotic block due to Rca1²/Rca1² is suppressed by Df(1)bi-D3/Df(1)bi-D3. Cell cycle arrest in CycA^C8LR1/CycA^C8LR1; Rca1²/Rca1² embryos, occurs earlier (cycle 15) than in either single mutant (both arrest at cycle 16). (Grosskortenhaus and Sprenger, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} (Zielke et al., 2006) Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{Δ133.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} (Zielke et al., 2006) Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} (Zielke et al., 2006) Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} (Zielke et al., 2006) Rca1² is rescued by Scer\GAL4^tub.PU/Rca1^{Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} (Zielke et al., 2006)
Partially rescued by	Rca1² is partially rescued by Rca1^{Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG}/Scer\GAL4^tub.PU (Zielke et al., 2006) Rca1² is partially rescued by Rca1^{ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG}/Scer\GAL4^tub.PU (Zielke et al., 2006) Rca1³/Rca1² is partially rescued by Scer\GAL4^prd.RG1/Rca1^{Scer\UAS.T:Ivir\HA1} (Grosskortenhaus and Sprenger, 2002)
Not rescued by	Rca1² is not rescued by Scer\GAL4^prd.RG1/Rca1^{C351S.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} (Zielke et al., 2006) Rca1² is not rescued by Scer\GAL4^prd.RG1/Rca1^{Δ255.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} (Zielke et al., 2006)
Comments	Expression of Rca1[Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG], Rca1[Δ133.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG], Rca1[Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] or Rca1[ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] under the control of Scer\GAL4[prd.RG1] in a Rca1[2] mutant background restores embryonic cell numbers to wild-type levels in Scer\GAL4[prd.RG1]-expressing segments. Expression of Rca1[Δ255.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] or Rca1[C351S.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] under the control of Scer\GAL4[prd.RG1] in a Rca1[2] mutant background fails to restore embryonic cell numbers to wild-type levels in Scer\GAL4[prd.RG1]-expressing segments. Expression of Rca1[Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] under the control of Scer\GAL4[tub] in Rca1[2] mutant wing disc clones suppresses the proliferation disadvantage observed in Rca1[2] mutant tissue, and clones are similar in size to wild-type. No endoreplication is observed in these cells and nuclear size is comparable to wild-type cells. Expression of Rca1[Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] or Rca1[ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG] under the control of Scer\GAL4[tub] in Rca1[2] mutant wing disc clones partially suppresses the proliferation disadvantage observed in Rca1[2] mutant tissue. Clones are increased in size compared to Rca1[2] mutant clones, but are only a third of the size of wild-type clones. Rarely, endoreplication is observed in mutant clones. (Zielke et al., 2006) (with Rca1³) Partially rescues: { Rca1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^prd.RG1 } In Rca1²/Rca1³; Rca1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^prd.RG1 embryos, mitosis 16 is rescued in alternating segments resulting in almost twice as many cells in mutant segments as in rescued ones. (Grosskortenhaus and Sprenger, 2002)
Stocks ( 1 )
Bloomington	9161 Rca1² cn¹ bw¹/SM6a
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Rca1² rca1² (Zielke et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Zielke et al., 2006, EMBO Rep. 7(12): 1266--1272 Molecular dissection of the APC/C inhibitor Rca1 shows a novel F-box-dependent function. [FBrf0193300] Grosskortenhaus and Sprenger, 2002, Dev. Cell 2(1): 29--40 Rca1 inhibits APC-Cdh1(Fzr) and is required to prevent Cyclin degradation in G2. [FBrf0141469] Dong et al., 1997, Genes Dev. 11(1): 94--105 Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A. [FBrf0091048]

Allele Dmel\Rca12

Allele Dmel\Rca1²