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General Information
Symbol
Dmel\Ras85DG13Q.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0058376
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Ras1Act, RAS1Act
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of an activated form of Ras85D (carries the amino acid replacement G13Q) is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Ras85DG13Q.Scer\UAS under the control of Scer\GAL4twi.PG results in extra eve-expressing progenitors in the dorsal mesoderm.

Supernumerary muscle founder cells are formed in embryos expressing Ras85DG13Q.Scer\UAS under the control of Scer\GAL4twi.PB.

Expression of Ras85DG13Q.Scer\UAS under the control of Scer\GAL4unspecified results in the production of extra eve-positive mesodermal cells in a wild-type background.

Ras85DG13Q.Scer\UAS expressed under the control of Scer\GAL4twi.PG in a wild-type background has no effect on early mesodermal migration.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

When Ras85DG13Q.Scer\UAS and Nintra.hs.Scer\UAS (driven by Scer\GAL4twi.PB) are combined an intermediate phenotype is seen, in which some hemisegments have excess eve progenitors and some have none.

Co-expression of wgScer\UAS.cGa and Ras85DG13Q.Scer\UAS under the control of Scer\GAL4twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twiScer\UAS.cBa and Ras85DG13Q.Scer\UAS under the control of Scer\GAL4twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twiScer\UAS.cBa, Ras85DG13Q.Scer\UAS and wgScer\UAS.cGa under the control of Scer\GAL4twi.PG results in an almost continuous anteroposterior stripe of eve-expressing progenitor cells confined to the dorsal mesoderm.

An intermediate number of muscle founder cells are produced when both htlDN.Scer\UAS.cMb and Ras85DG13Q.Scer\UAS are co-expressed under the control of Scer\GAL4twi.PB compared to when either htlDN.Scer\UAS.cMb or Ras85DG13Q.Scer\UAS alone is expressed under the control of Scer\GAL4twi.PB (no muscle founder cells or supernumerary muscle founder cells respectively).

The production of extra eve-positive mesodermal cells caused by expression of Ras85DG13Q.Scer\UAS under the control of Scer\GAL4unspecified is suppressed if the embryos are also carrying dppH46 or wgunspecified. Expression of Ras85DG13Q.Scer\UAS under the control of Scer\GAL4unspecified results in the production of extra eve-positive mesodermal cells in mbcunspecified or stg2 background.

The failure of mesodermal migration in early htlAB42 embryos is partially rescued by Ras85DG13Q.Scer\UAS expressed under the control of Scer\GAL4twi.PG.

Partially rescues the stumpsYY202 phenotype when expressed under the control of Scer\GAL4twi.PG.

Partially rescues the htlZZ81 phenotype when expressed under the control of Scer\GAL4twi.PG.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Ras85DG13Q.Scer\UAS
Ras85DG13Q.UAS
Name Synonyms
Secondary FlyBase IDs
    References (19)