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General Information
Symbol
Dmel\Pi3K92EUAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0058396
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dp110, UAS-PI3K, UAS-p110, UAS-dPI3K, UAS Dp110, UASDp110
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a cDNA fragment encoding the wild-type Pi3K92E coding region. The open reading frame is tagged at the N-terminal end with Tag:MYC.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
cell surface & nurse cell | somatic clone, with Scer\GAL4Act5C.PP
imaginal disc & cell, with Scer\GAL4Act5C.PP
Detailed Description
Statement
Reference
Somatic clones in the larval fat body expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C display increased fat body cell size and lipid droplet diameter during starvation conditions, as compared to controls. Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Desat1.PB in larvae results in increased size of oenocytes in both fed and starvation conditions, and during starvation, oenocyte nuclei undergo higher levels of endoreplication, as compared to controls.
Over-expression of Pi3K92EScer\UAS.T:Hsap\MYC driven by Scer\GAL4da.PU results in a significant decrease in lifespan, compared to controls.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc.PU in cells of the wing disc proper has no significant effect on the morphology or proliferation of the overlying peripodial membrane cells in third instar larvae.
Scer\GAL4Dl-NP0677-mediated expression increases the number of intestinal stem cells in the adult midgut.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF results in significant eye overgrowth.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC in the mushroom bodies under the control of Scer\GAL4Tab2-201Y causes an increase in the size of the mushroom body lobes and calyces, compared with controls. These synaptic changes do not have a detectable consequence on odour central adaptation. Expression of Pi3K92EScer\UAS.T:Hsap\MYC in the projection neurons, under the control of Scer\GAL4GH146 or Scer\GAL4exba-krasavietz, does not affect central or cross adaptation to EB, IAA and benzaldehyde odours. Coincident odorant-specific DM2 glomerular size change induced by exposure to EB is not detected when Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GH298.
Scer\GAL4elav.PLu-mediated expression of Pi3K92EScer\UAS.T:Hsap\MYC produces substantial synaptic overgrowth at the larval neuromuscular junction and increased excitatory junctional potentials. However, no significant effect is seen on axon guidance in the visual system or on phototaxis behaviour.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU at 30[o]C leads to a significant increase in eye size and to a disorganised retina, compared to controls.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4AB1 results in a 186% increase in the size of the salivary gland in larvae, which is principally mediated by an increase in cell size.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in a 15-20% increase in the surface area of the wing.
No scutellar bristle phenotypes are seen when Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4sca-537.4. Expression in the wing under the control of Scer\GAL4en-e16E leads to over-growth of the posterior compartment.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF leads to an increase in ommatidia size.
The salivary glands of animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4fkh.PH are greatly increased in area compared to controls at 13.5 hours after puparium formation (APF). At 24 hours APF, animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4fkh.PH still contain salivary gland tissue, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage. The salivary glands in the mutant animals are always present at 24 hours APF, but are partly degraded. The induction of autophagy that is seen in wild-type salivary glands at 13.5 hours APF is not seen in animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4fkh.PH. DNA fragmentation is detected by TUNEL in salivary glands of animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4fkh.PH at 13.5 hours after puparium formation, as occurs in control salivary glands at this stage.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4nub-AC-62 results in tissue overgrowth in the larval salivary glands.
80% of pupae expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.PB (using a 30 minute heat shock at 9 hours after puparium formation) still possess intact salivary glands at 20 hours after puparium formation (this is approximately 6 hours after the glands are normally destroyed in wild-type animals).
Larval expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4D42 results in twice as many synapses as wild-type. Spontaneous release from motor neurons overexpressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4D42 is increased by approximately 40% compared to controls in muscle fibers F6 and F7. The mean amplitude of spontaneous release in these neurons is also increased by 40% with respect to controls. The evoked release, measured as stimulus-induced EPSPs, also produces a 22% increase in motor neurons overexpressing Pi3K92EScer\UAS.T:Hsap\MYC. Overexpression of Pi3K92EScer\UAS.T:Hsap\MYC in R1-3, R4m and R4d cholinergic neurons under the control of Scer\GAL4796 results in a 56% increase in the size of the ellipsoid body. The synaptic domain of Pi3K92E-expressing neurons is, however, more than threefold larger, a much larger increase compared with the change in cell size. Pi3K92EScer\UAS.T:Hsap\MYC expression induces a thicker axon diameter and a wider crown of projections with more abundant synaptic boutons. The number of Scer\GAL4796 neurons is statistically similar in controls and flies overexpressing Pi3K92EScer\UAS.T:Hsap\MYC. Flies overexpressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4796 display 18% higher density of cell branch profiles and small clear vesicles, compared with controls, reflecting a 24% increase in synaptic density. The estimated total number of synapses in the ellipsoid body of Pi3K92EScer\UAS.T:Hsap\MYC animals is 64% higher than controls. These flies show a normal maturation profile, albeit with values consistently higher than the sibling controls. Animals overexpressing Pi3K92EScer\UAS.T:Hsap\MYC in ellipsoid body neurons under the control of Scer\GAL4796 are active for longer periods and, consequently, walk longer distances than their sibling controls. The number of full walks is also higher in Pi3K92EScer\UAS.T:Hsap\MYC animals, while walking speed is unaffected. When Pi3K92EScer\UAS.T:Hsap\MYC overexpression under the control of Scer\GAL4796 is blocked through Scer\GAL80ts.αTub84B, supernumerary synapses are rapidly eliminated to give levels comparable to wild-type, indicating that high levels of Pi3K92E generated by Pi3K92EScer\UAS.T:Hsap\MYC overexpression are necessary not only for the formation of ectopic synapses but also for subsequent synapse maintenance. Adult Pi3K92EScer\UAS.T:Hsap\MYC/Scer\GAL4796 overexpression flies that have been raised at a permissive temperature in a Scer\GAL80ts.αTub84B background (so Scer\GAL4796-driven expression is suppressed) and then later places at a restrictive temperature (where Scer\GAL80ts.αTub84B expression is suppressed so Scer\GAL4796-driven expression of Pi3K92EScer\UAS.T:Hsap\MYC is enabled), achieve a two-fold increase of synaptic domain. This is a lower increase than in flies with constitutive Pi3K92EScer\UAS.T:Hsap\MYC expression, indicating a loss of neuronal plasticity. Constitutive Pi3K92EScer\UAS.T:Hsap\MYC overexpression (under the control of Scer\GAL4796) increases dendritic tuft branching compared to controls. Pi3K92EScer\UAS.T:Hsap\MYC switch-on in 30 day old flies also increases dendritic branching in 52-day old flies.
Nurse cell clones that express Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP show a normal distribution of lipid droplets. However, 16% of egg chambers with these clones exhibit elongated cell surface lipid droplet-type structures in nurse cells at the periphery of the egg chamber, which are not seen in wild-type egg chambers.
When Pi3K92EScer\UAS.T:Hsap\MYC is driven by Scer\GAL4Aug21 no effect on adult size is observed. Expression of Pi3K92EScer\UAS.T:Hsap\MYC in the ring gland using Scer\GAL4P0206 induces autonomous growth.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC using Scer\GAL4P0206 induces autonomous growth in the ring gland, especially in the prothoracic gland. The whole adults show reduced growth at all stages of development and emerging adults with reduced size and body weight. This decrease was attributable to an decrease in cell number in the wing and a decrease in cell number in eye. The timing of embryonic and larval development in these mutants is comparable to controls. The duration of pupal development is delayed by less than four hours.
Overexpression of Pi3K92EScer\UAS.T:Hsap\MYC under the regulation of Scer\GAL4dpp.blk1 does not appreciably alter nucleolar size.
Scer\GAL4GMR.long>Pi3K92EScer\UAS.T:Hsap\MYC mutants show no defects in opsin regulation.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC in the prothoracic gland, driven by Scer\GAL4phm.PO, causes enlarged prothoracic gland cells during all feeding stages. Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC pharate adults are 73% (females) and 80% (males) the weight of controls. This phenotype is enhanced when larvae are reared under constant light (LL) as pharate adults are then 51% the weight of controls and pupariate ~4 hours earlier than controls reared under 14 hours light:10 hours dark. Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC flies are the same size as controls at hatching, but grow more slowly than controls during L1 and L2. However, when Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC larvae are reared under LL on a yeast-supplemented diet they grow at the same rate as controls. Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC larvae overestimate their size so that they initiate metamorphosis before reaching the minimal viable weight necessary to survive pupation; 50% of Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC larvae are able to pupariate after starvation at 2.6 hr after L3 ecdysis (AL3E) when they are only 0.36mg, instead of pupariating at 11.4 hours at a weight of 0.86mg like controls. While control larvae do not pupariate under starvation conditions unless they have been fed for at least 10 hours AL3E, Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC larvae can pupariate at 0 hour starvation. This results in the formation of partial puparia that are tanned but have failed to contract properly, although they are competent to undergo some of the earliest metamorphic processes such as eversion and elongation. However, such puparia are non-viable and any larvae that fail to pupariate at this time die. When Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC are starved after 4 hours AL3E, 73% form normal puparia, 13% form partial puparia, and the remaining larvae die. These animals exhibit molting defects in the prepupal stages. Scer\GAL4phm.PO>Pi3K92EScer\UAS.T:Hsap\MYC flies starved at 8 hours AL3E form normal puparia and do not exhibit molting defects. Expression of Pi3K92EScer\UAS.T:Hsap\MYC, under the control of either Scer\GAL4P0206 or Scer\GAL4Aug21, does not affect body size. However, Scer\GAL4P0206>Pi3K92EScer\UAS.T:Hsap\MYC pharate adults reared under constant light (LL) are 80% the size of control pharate adults grown under the same conditions.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC, under the control of Scer\GAL429BD, causes overgrown salivary glands and wing discs in third instar larvae.
Fasted third instar larvae expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4NPFR1.6.6 display an attenuated feeding response to solid food. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls.
Flies overexpressing Pi3K92EScer\UAS.T:Hsap\MYC in dopaminergic neurons (through Scer\GAL4ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP in clones in the eye disc results in enlarged cells with rotation defects. Precocious differentiation of cells in the eye disc is also seen.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in larger wings than normal.
When expression is governed by Scer\GAL4Lsp2.PH there is a noticeable lack of large autolysosomes. In state similar to when ecdysone signalling is blocked the cytoplasm contains small autophagosomes and autolysosomes. Glycogen accumulates, probably due to a failure of autophagy to degrade glycogen. Autophagic area decreased compared to wild type.
Expression driven by Scer\GAL4Act5C.PP in clones in the fat body suppresses starvation-induced autophagy, but not the autophagy induced by TorΔP loss of function.
Somatic clones expressing dmScer\UAS.cZa (driven by Scer\GAL4αTub84B.PC, in the wing disc) are significantly larger than controls. Sibling clones next to these clones are similar in size to controls elsewhere. When Pi3K92EScer\UAS.T:Hsap\MYC is driven by Scer\GAL4dpp.blk1 or Scer\GAL4αTub84B.PC lead to wings that are significantly larger than controls.
Pi3K92EScer\UAS.T:Hsap\MYC driven by Scer\GAL4HLHmδ has no effect on ommatidial rotation.
Pi3K92EScer\UAS.T:Hsap\MYC; Scer\GAL4en-e16E animals are hyper-sensitive to starvation.
Caspase activation in cells posterior to the furrow in eye discs is unaffected by Pi3K92EScer\UAS.T:Hsap\MYC; Scer\GAL4GMR.PF.
Clones of cells expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP in the gut, fat body, Malpighian tubules and epidermis are only slightly larger than control cells at the time of larval hatching. After several days of starvation on 20% sucrose, these cells are much larger than adjacent control cells and have a visibly increased DNA content. Gut and fat body cells expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP continue to replicate their DNA for at least 2-3 days under starvation conditions. Expression of Pi3K92EScer\UAS.T:Hsap\MYC in third larval instar fat body cells under the control of Scer\GAL4Act5C.PP increases the opacity of the cytoplasm, thus promoting nutrient storage. Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Adh.PF in fed animals does not affect survival. In contrast, expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Adh.PF in starved animals results in first instar larvae dying within 3-4 days of hatching (control animals survive 8-9 days). Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E in starved animals results in them dying within 2-3 days, 4-5 days before controls. Animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Adh.PF feed poorly, often having no food in their gut, and frequently wander away from their food.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in a slight increase in wing size and in males the wings show a downward curvature.
Clones of cells expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP are 28% larger than control cells.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP in wing disc cells results in an increase in cell size compared to controls. The proportion of cells in G1 is decreased compared to controls. Clones expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP are not visibly round, do not generate ectopic folds and do not bulge out of the epithelium.
Animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF have a significantly larger eye than normal, due to an increase in ommatidia size. Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4ap-md544 results in dramatic overgrowth of the wing, inhibiting wing unfolding. Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 causes the wing to bend downwards slightly.
When Pi3K92ED954A.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4ptc-559.1 results in a increase in the distance between the L3 and L4 veins in the wing.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4dpp.blk1 results in enlargement of the region encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC driven by Scer\GAL4Bx-MS1096 results in an increased wing size.
When expression of Pi3K92EScer\UAS.T:Hsap\MYC is driven by Scer\GAL4en-e16E most animals die as larvae or pupae with the few survivors having distorted or crumpled wings. The posterior compartment of these wings is greatly enlarged relative to the anterior compartment.
When expression is driven in imaginal disc cells by Scer\GAL4Act5C.PP the size of the cells is increased (as measured by FSC (forward scatter)). Cells are larger during G1 and the S+G2 phase. Overexpression of Pi3K92EScer\UAS.T:Hsap\MYC is not sufficient to increase cell number. When expression is driven by Scer\GAL4en-e16E, the size of the posterior compartment of the wing disc is significantly increased. There is no comparable effect on the anterior compartment.
Scer\GAL4dpp.blk1 mediated expression caused expansion of the wing margin due to an increase in overall number of cells and their size. Scer\GAL4GMR.PF mediated expression causes enlarged and bulging roughened eyes with fused ommatidia and misplaced or duplicated bristles.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
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Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
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Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
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Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of Pi3K92EScer\UAS.T:Hsap\MYC suppresses embryonic lethality seen with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU. Co-expression of Pi3K21BScer\UAS.T:Ivir\HA1 suppresses the small body phenotype in larvae with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU, but larvae remain first instars for 2 weeks until they die and retain fat body abnormalities.
Co-expression of Ptp61FScer\UAS.n.T:Hsap\MYC has no overt effect on the eye overgrowth phenotype caused by expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF.
dj-1βΔ93/dj-1βΔ93 does not suppress the eye phenotypes (including increased eye size) in flies with expression of Pi3K92EScer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU at 30[o]C.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4AB1 in a trpm28 background does not result in an increase in cell size in the larval salivary gland.
Co-expression of Pi3K92EScer\UAS.T:Hsap\MYC does not suppress the ability of AcnScer\UAS.T:Hsap\MYC expressed under the control of Scer\GAL4Lsp2.PH to induce autolysosome formation in the fat body in the absence of starvation.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC enhances the rough eye phenotype seen when Dcp-1Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4GMR.PF.
Neuroblasts are detected in the mushroom body of 2 week old adults expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4wor.PA in a Df(3L)H99/Df(3L)XR38 background (mushroom body neuroblasts are not seen in wild-type adults).
The eye phenotype in Ras85DKP mutant flies is not suppressed by ectopic expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF.
Co-expression of HLH106Scer\UAS.Exel and Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in flies with severely deformed wings.
Expression of RpL8dsRNA.Scer\UAS partially suppresses the increase in ommatidia size seen when Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PF.
Co-expression of TorTED.Scer\UAS suppresses the salivary gland overgrowth phenotype that is seen in animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4fkh.PH and at 24 hours after puparium formation the salivary glands are predominantly degraded in the double mutant animals. The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4fkh.PH is significantly enhanced if the animals are also carrying NcI24/NcI29.
The overgrowth of the salivary gland that is seen larvae expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4nub-AC-62 is suppressed by rictorΔ2.
The persistence of salivary glands seen in pupae expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.PB (using a 30 minute heat shock at 9 hours after puparium formation) is still seen if the animals are also heterozygous or homozygous for foxo25.
Pi3K92EScer\UAS.T:Hsap\MYC; Scer\GAL4Lsp2.PH suppresses the reduced triglyceride phenotype see in Df(3L)meltΔ1 homozygous embryos.
Coexpression of Pi3K92EScer\UAS.T:Hsap\MYC with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF suppresses the DJ-1αdsRNA.Scer\UAS-induced eye phenotype. The eyes are restored to normal size, and the organisation of the ommatidia is significantly improved.
DJ-1αdsRNA.Scer\UAS flies coexpressing Pi3K92EScer\UAS.T:Hsap\MYC (both in the dopaminergic neurons through Scer\GAL4ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type.
Co-expression of Pi3K92EScer\UAS.T:Hsap\MYC almost completely rescues the eye phenotype caused by expression of foxoScer\UAS.cKa under the control of Scer\GAL4GMR.PF; the ommatidia and nearly all of the mechanosensory bristles are restored. Co-expression of Pi3K92EScer\UAS.T:Hsap\MYC partially rescues the eye phenotype caused by expression of Mmus\Foxo1AA.Scer\UAS under the control of Scer\GAL4GMR.PF; a lack of ommatidia and mechanosensory bristles is still seen.
The hyper-sensitivity of Pi3K92EScer\UAS.T:Hsap\MYC; Scer\GAL4en-e16E animals to starvation is partially suppressed by RhebPΔ1/+ or RhebPΔ2/+ and more completely suppressed by RhebPΔ1/RhebPΔ2.
Coexpression of TorWT.Scer\UAS.T:Zzzz\FLAG and Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in a severe reduction in wing size.
Co-expression of S6kScer\UAS.cMa does not result in any further increase in eye size in animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF. Co-expression of Akt1Scer\UAS.cRa results in a clear increase the size of the eye and individual ommatidia in animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF. Co-expression of S6kScer\UAS.cMa does not modify the bent wing phenotype caused by expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096. Co-expression of Akt1Scer\UAS.cRa in animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 causes the wing to bend strongly downwards. The enlarged eye and ommatidia phenotype caused by expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF is not affected if the flies are also homozygous for S6kl-1. The enlarged eye and ommatidia phenotype caused by expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF is completely suppressed in a Akt11/Akt13 background.
Flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC and PtenScer\UAS.cGb under the control of Scer\GAL4Bx-MS1096 have wings even smaller than flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC or PtenScer\UAS.cGb alone.
Flies expressing both Pi3K92EScer\UAS.T:Hsap\MYC and PtenScer\UAS.cGa driven by Scer\GAL4en-e16E show a much higher viability than those expressing either alone. The posterior compartment of the wing is nearly as reduced in area as in animals expressing PtenScer\UAS.cGa driven by Scer\GAL4en-e16E alone.
Suppresses the eye phenotype caused by PtenScer\UAS.cHa, when co-expressed using Scer\GAL4ey.PH.
Xenogenetic Interactions
Statement
Reference
Expression of Pi3K92EScer\UAS.T:Hsap\MYC suppresses the retinal phenotype seen when Hsap\HTT128Q.1-336.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU. Expression of Pi3K92EScer\UAS.T:Hsap\MYC enhances the retinal phenotype seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC is unable to suppress the growth phenotypes seen in the wings and bristles of flies expressing Hsap\TGIF2LXScer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E or Scer\GAL4sca-537.4 respectively.
Expression of Hsap\RPL8DN.Scer\UAS partially suppresses the increase in ommatidia size seen when Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PF.
The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when one of BacA\p35Scer\UAS.cHa or Pi3K92EScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4fkh.PH is significantly increased when both BacA\p35Scer\UAS.cHa and Pi3K92EScer\UAS.T:Hsap\MYC are co-expressed under the control of Scer\GAL4fkh.PH.
Coexpression of Pi3K92EScer\UAS.T:Hsap\MYC with Hsap\MAPTV337M.Scer\UAS, under the control of Scer\GAL4GMR.PF has no effect on the Hsap\MAPTV337M.Scer\UAS-induced toxicity in the eye.
The addition of Pi3K92EScer\UAS.T:Hsap\MYC dramatically worsens the Hsap\ATX182Q.Scer\UAS (driven by Scer\GAL4GMR.PF) neurodegeneration phenotype in the eye.
Complementation and Rescue Data
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Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Pi3K92EScer\UAS.T:Hsap\MYC
Pi3K92EUAS.T:Myc
Pi3K92EUAS.Tag:MYC
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    References (72)