Allele Dmel\Pi3K92E^{Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\Pi3K92E^{Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0058396
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\Pi3K92E

Allele class
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| coding region fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Leevers et al., 1996) in vitro construct \| coding region fusion (Leevers et al., 1996)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a wild-type cDNA fragment encoding Pi3K92E coding regions tagged at the N-terminus with Myc is influenced by a non-heat shock responsive Hsp70 promoter and five Scer\UAS Scer\GAL4 binding sites. (Leevers et al., 1996)
Assay mode	In transgenic Drosophila (intraspecific) (Leevers et al., 1996, Yang et al., 2005, Wu et al., 2005, Yang, 2005)
Carried in construct	P{WT-Dp110} (Yang et al., 2005, Berry and Baehrecke, 2007, Liu and Lehmann, 2006, Mirth et al., 2005, Radimerski et al., 2002, Colombani et al., 2005, Saucedo et al., 2003, Takeo et al., 2005, Kramer et al., 2003, Britton et al., 2002, Wu et al., 2005, Xue and Noll, 2002, Colombani et al., 2005, Yang, 2005, Hennig and Neufeld, 2002, Hipfner et al., 2002, Prober and Edgar, 2002, Brumby and Richardson, 2003, de la Cova et al., 2004, Gaengel and Mlodzik, 2003, Teleman et al., 2005, Huang et al., 1999, Gao et al., 2000, Weinkove et al., 1999, Sustar and Schubiger, 2005, Scott et al., 2004, Rusten et al., 2004, Bateman and McNeill, 2004, Miron et al., 2001, Cho et al., 2001, Yang and Baker, 2003, Chen et al., 2003, Leevers et al., 1996, Mikeladze-Dvali et al., 2005, Betschinger et al., 2006, Vereshchagina and Wilson, 2006, Hietakangas and Cohen, 2007)
Tagged with	T:Hsap\MYC (Weinkove et al., 1999, Huang et al., 1999, Gao et al., 2000, Miron et al., 2001, Cho et al., 2001, Britton et al., 2002, Xue and Noll, 2002, Radimerski et al., 2002, Prober and Edgar, 2002, Hipfner et al., 2002, Hennig and Neufeld, 2002, Yang and Baker, 2003, Chen et al., 2003, Kramer et al., 2003, Saucedo et al., 2003, Gaengel and Mlodzik, 2003, Brumby and Richardson, 2003, de la Cova et al., 2004, Bateman and McNeill, 2004, Scott et al., 2004, Rusten et al., 2004, Sustar and Schubiger, 2005, Grewal et al., 2005, Takeo et al., 2005, Wu et al., 2005, Yang et al., 2005, Mirth et al., 2005, Colombani et al., 2005, Colombani et al., 2005, Yang, 2005, Leevers et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	viable, with Scer\GAL4^Adh.PF (Britton et al., 2002) viable, with Scer\GAL4^en-e16E (Britton et al., 2002) lethal, with Scer\GAL4^Act5C.PP (Britton et al., 2002) visible, with Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002, Hennig and Neufeld, 2002) visible, with Scer\GAL4^dpp.blk1 (Miron et al., 2001) visible, with Scer\GAL4^ap-md544 (Radimerski et al., 2002) visible, with Scer\GAL4^GMR.PF (Radimerski et al., 2002) small body \| second instar larval stage, with Scer\GAL4^phm.PO (Mirth et al., 2005) small body \| third instar larval stage, with Scer\GAL4^phm.PO (Mirth et al., 2005) small body \| pharate adult stage, with Scer\GAL4^P0206 (Mirth et al., 2005) small body, with Scer\GAL4^P0206 (Colombani et al., 2005) reduced cell number, with Scer\GAL4^P0206 (Colombani et al., 2005) reduced cell size, with Scer\GAL4^P0206 (Colombani et al., 2005) increased cell size \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2002, Bateman and McNeill, 2004, Britton et al., 2002, Hipfner et al., 2002) increased cell size, with Scer\GAL4^Bx-MS1096 (Gao et al., 2000) fertile, with Scer\GAL4^Adh.PF (Britton et al., 2002) fertile, with Scer\GAL4^en-e16E (Britton et al., 2002) environmental stress response defective, with Scer\GAL4^en-e16E (Saucedo et al., 2003) feeding behavior defective, with Scer\GAL4^Adh.PF (Britton et al., 2002) feeding behavior defective, with Scer\GAL4^NPFR1.6.6 (Wu et al., 2005) photoperiod response variant, with Scer\GAL4^phm.PO (Mirth et al., 2005) cell death decrease, with Scer\GAL4^hs.PB (Liu and Lehmann, 2006) cell growth defective \| somatic clone, with Scer\GAL4^Act5C.PP (de la Cova et al., 2004) cell growth defective, with Scer\GAL4^P0206 (Colombani et al., 2005, Colombani et al., 2005) cell size defective, with Scer\GAL4^phm.PO (Mirth et al., 2005) hyperplastic, with Scer\GAL4^nub-AC-62 (Hietakangas and Cohen, 2007) tissue polarity defective \| somatic clone, with Scer\GAL4^Act5C.PP (Bateman and McNeill, 2004) developmental rate defective, with Scer\GAL4^P0206 (Colombani et al., 2005) developmental rate defective, with Scer\GAL4^phm.PO (Mirth et al., 2005)
Phenotype Manifest In
	imaginal disc & cell, with Scer\GAL4^Act5C.PP (Weinkove et al., 1999) fat body, with Scer\GAL4^Act5C.PP (Scott et al., 2004) mitotic cell cycle \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2002) ommatidial cluster \| somatic clone, with Scer\GAL4^Act5C.PP (Bateman and McNeill, 2004) eye disc \| somatic clone, with Scer\GAL4^Act5C.PP (Bateman and McNeill, 2004) larval fat body \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) larval digestive system \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) larval Malpighian tubule \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) larval epidermis \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) cell surface & nurse cell \| somatic clone, with Scer\GAL4^Act5C.PP (Vereshchagina and Wilson, 2006) dorsal mesothoracic disc \| posterior compartment, with Scer\GAL4^en-e16E (Weinkove et al., 1999) ring canal, with Scer\GAL4^P0206 (Colombani et al., 2005, Colombani et al., 2005) prothoracic gland, with Scer\GAL4^P0206 (Colombani et al., 2005) wing, with Scer\GAL4^P0206 (Colombani et al., 2005) eye, with Scer\GAL4^P0206 (Colombani et al., 2005) eye, with Scer\GAL4^GMR.PF (Radimerski et al., 2002, Chen et al., 2003, Leevers et al., 1996) ommatidium, with Scer\GAL4^GMR.PF (Radimerski et al., 2002, Leevers et al., 1996) interommatidial bristle, with Scer\GAL4^GMR.PF (Leevers et al., 1996) wing, with Scer\GAL4^ap-md544 (Radimerski et al., 2002) wing, with Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002, Gao et al., 2000, Hennig and Neufeld, 2002) larval salivary gland, with Scer\GAL4^29BD (Takeo et al., 2005) dorsal mesothoracic disc, with Scer\GAL4^29BD (Takeo et al., 2005) wing, with Scer\GAL4^dpp.blk1 (Leevers et al., 1996, de la Cova et al., 2004) 1st posterior cell, with Scer\GAL4^dpp.blk1 (Miron et al., 2001) 1st posterior cell, with Scer\GAL4^ptc-559.1 (Cho et al., 2001) wing, with Scer\GAL4^αTub84B.PC (de la Cova et al., 2004) prothoracic gland, with Scer\GAL4^phm.PO (Mirth et al., 2005) puparium, with Scer\GAL4^phm.PO (Mirth et al., 2005) lysosome, with Scer\GAL4^Lsp2.PH (Rusten et al., 2004) autophagic vacuole, with Scer\GAL4^Lsp2.PH (Rusten et al., 2004) larval salivary gland, with Scer\GAL4^hs.PB (Liu and Lehmann, 2006) larval salivary gland, with Scer\GAL4^nub-AC-62 (Hietakangas and Cohen, 2007) larval salivary gland, with Scer\GAL4^fkh.PH (Berry and Baehrecke, 2007)
Detailed Description
	Statement Reference Scer\GAL4^dpp.blk1 mediated expression caused expansion of the wing margin due to an increase in overall number of cells and their size. Scer\GAL4^GMR.PF mediated expression causes enlarged and bulging roughened eyes with fused ommatidia and misplaced or duplicated bristles. (Leevers et al., 1996) When expression is driven in imaginal disc cells by Scer\GAL4^Act5C.PP the size of the cells is increased (as measured by FSC (forward scatter)). Cells are larger during G1 and the S+G2 phase. Overexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} is not sufficient to increase cell number. When expression is driven by Scer\GAL4^en-e16E, the size of the posterior compartment of the wing disc is significantly increased. There is no comparable effect on the anterior compartment. (Weinkove et al., 1999) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^Bx-MS1096 results in an increased wing size. (Gao et al., 2000) When Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^ptc-559.1 results in a increase in the distance between the L3 and L4 veins in the wing. (Cho et al., 2001) Clones of cells expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in the gut, fat body, Malpighian tubules and epidermis are only slightly larger than control cells at the time of larval hatching. After several days of starvation on 20% sucrose, these cells are much larger than adjacent control cells and have a visibly increased DNA content. Gut and fat body cells expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP continue to replicate their DNA for at least 2-3 days under starvation conditions. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in third larval instar fat body cells under the control of Scer\GAL4^Act5C.PP increases the opacity of the cytoplasm, thus promoting nutrient storage. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Adh.PF in fed animals does not affect survival. In contrast, expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Adh.PF in starved animals results in first instar larvae dying within 3-4 days of hatching (control animals survive 8-9 days). Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en-e16E in starved animals results in them dying within 2-3 days, 4-5 days before controls. Animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Adh.PF feed poorly, often having no food in their gut, and frequently wander away from their food. (Britton et al., 2002) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in wing disc cells results in an increase in cell size compared to controls. The proportion of cells in G₁ is decreased compared to controls. Clones expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP are not visibly round, do not generate ectopic folds and do not bulge out of the epithelium. (Prober and Edgar, 2002) Clones of cells expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP are 28% larger than control cells. (Hipfner et al., 2002) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in a slight increase in wing size and in males the wings show a downward curvature. (Hennig and Neufeld, 2002) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1 results in enlargement of the region encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin. (Miron et al., 2001) Animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF have a significantly larger eye than normal, due to an increase in ommatidia size. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ap-md544 results in dramatic overgrowth of the wing, inhibiting wing unfolding. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 causes the wing to bend downwards slightly. (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PF leads to enlarged eyes. (Chen et al., 2003) Caspase activation in cells posterior to the furrow in eye discs is unaffected by Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^GMR.PF. (Yang and Baker, 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^en-e16E animals are hyper-sensitive to starvation. (Saucedo et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^HLHmδ has no effect on ommatidial rotation. (Gaengel and Mlodzik, 2003) Somatic clones expressing dm^Scer\UAS.cZa (driven by Scer\GAL4^αTub84B.PC, in the wing disc) are significantly larger than controls. Sibling clones next to these clones are similar in size to controls elsewhere. When Pi3K92E^{Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^dpp.blk1 or Scer\GAL4^αTub84B.PC lead to wings that are significantly larger than controls. (de la Cova et al., 2004) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in clones in the eye disc results in enlarged cells with rotation defects. Precocious differentiation of cells in the eye disc is also seen. (Bateman and McNeill, 2004) Expression driven by Scer\GAL4^Act5C.PP in clones in the fat body suppresses starvation-induced autophagy, but not the autophagy induced by Tor^ΔP loss of function. (Scott et al., 2004) When expression is governed by Scer\GAL4^Lsp2.PH there is a noticeable lack of large autolysosomes. In state similar to when ecdysone signalling is blocked the cytoplasm contains small autophagosomes and autolysosomes. Glycogen accumulates, probably due to a failure of autophagy to degrade glycogen. Autophagic area decreased compared to wild type. (Rusten et al., 2004) Overexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the regulation of Scer\GAL4^dpp.blk1 does not appreciably alter nucleolar size. (Grewal et al., 2005) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^29BD, causes overgrown salivary glands and wing discs in third instar larvae. (Takeo et al., 2005) Fasted third instar larvae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^NPFR1.6.6 display an attenuated feeding response to solid food. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls. (Wu et al., 2005) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} using Scer\GAL4^P0206 induces autonomous growth in the ring gland, especially in the prothoracic gland. The whole adults show reduced growth at all stages of development and emerging adults with reduced size and body weight. This decrease was attributable to an decrease in cell number in the wing and a decrease in cell number in eye. The timing of embryonic and larval development in these mutants is comparable to controls. The duration of pupal development is delayed by less than four hours. (Colombani et al., 2005) When Pi3K92E^{Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^Aug21 no effect on adult size is observed. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in the ring gland using Scer\GAL4^P0206 induces autonomous growth. (Colombani et al., 2005) Flies overexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} in dopaminergic neurons (through Scer\GAL4^ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type. (Yang, 2005) Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in the prothoracic gland, driven by Scer\GAL4^phm.PO, causes enlarged prothoracic gland cells during all feeding stages. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} pharate adults are 73% (females) and 80% (males) the weight of controls. This phenotype is enhanced when larvae are reared under constant light (LL) as pharate adults are then 51% the weight of controls and pupariate ~4 hours earlier than controls reared under 14 hours light:10 hours dark. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} flies are the same size as controls at hatching, but grow more slowly than controls during L1 and L2. However, when Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae are reared under LL on a yeast-supplemented diet they grow at the same rate as controls. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae overestimate their size so that they initiate metamorphosis before reaching the minimal viable weight necessary to survive pupation; 50% of Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae are able to pupariate after starvation at 2.6 hr after L3 ecdysis (AL3E) when they are only 0.36mg, instead of pupariating at 11.4 hours at a weight of 0.86mg like controls. While control larvae do not pupariate under starvation conditions unless they have been fed for at least 10 hours AL3E, Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae can pupariate at 0 hour starvation. This results in the formation of partial puparia that are tanned but have failed to contract properly, although they are competent to undergo some of the earliest metamorphic processes such as eversion and elongation. However, such puparia are non-viable and any larvae that fail to pupariate at this time die. When Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} are starved after 4 hours AL3E, 73% form normal puparia, 13% form partial puparia, and the remaining larvae die. These animals exhibit molting defects in the prepupal stages. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} flies starved at 8 hours AL3E form normal puparia and do not exhibit molting defects. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, under the control of either Scer\GAL4^P0206 or Scer\GAL4^Aug21, does not affect body size. However, Scer\GAL4^P0206>Pi3K92E^{Scer\UAS.T:Hsap\MYC} pharate adults reared under constant light (LL) are 80% the size of control pharate adults grown under the same conditions. (Mirth et al., 2005) Scer\GAL4[GMR.long]>Pi3K92E[Scer\UAS.T:Hsap\MYC] mutants show no defects in opsin regulation. (Mikeladze-Dvali et al., 2005) 80% of pupae expressing Pi3K92E[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[hs.PB] (using a 30 minute heat shock at 9 hours after puparium formation) still possess intact salivary glands at 20 hours after puparium formation (this is approximately 6 hours after the glands are normally destroyed in wild-type animals). (Liu and Lehmann, 2006) Nurse cell clones that express Pi3K92E[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Act5C.PP] show a normal distribution of lipid droplets. However, 16% of egg chambers with these clones exhibit elongated cell surface lipid droplet-type structures in nurse cells at the periphery of the egg chamber, which are not seen in wild-type egg chambers. (Vereshchagina and Wilson, 2006) Expression of Pi3K92E[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[nub-AC-62] results in tissue overgrowth in the larval salivary glands. (Hietakangas and Cohen, 2007) The salivary glands of animals expressing Pi3K92E[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[fkh.PH] are greatly increased in area compared to controls at 13.5 hours after puparium formation (APF). At 24 hours APF, animals expressing Pi3K92E[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[fkh.PH] still contain salivary gland tissue, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage. The salivary glands in the mutant animals are always present at 24 hours APF, but are partly degraded. The induction of autophagy that is seen in wild-type salivary glands at 13.5 hours APF is not seen in animals expressing Pi3K92E[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[fkh.PH]. DNA fragmentation is detected by TUNEL in salivary glands of animals expressing Pi3K92E[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[fkh.PH] at 13.5 hours after puparium formation, as occurs in control salivary glands at this stage. (Berry and Baehrecke, 2007)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 has visible phenotype, enhanceable by Akt1^Scer\UAS.cRa, Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Akt1^Scer\UAS.cRa, Scer\GAL4^GMR.PF (Radimerski et al., 2002)
NOT Enhanced by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 has visible phenotype, non-enhanceable by S6k^Scer\UAS.cMa, Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by S6k^l-1, Scer\GAL4^GMR.PF (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by S6k^Scer\UAS.cMa, Scer\GAL4^GMR.PF (Radimerski et al., 2002)
Suppressed by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^P0206 has reduced cell number phenotype, suppressible by foxo²¹ (Colombani et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^P0206 has reduced cell size phenotype, suppressible by foxo²¹ (Colombani et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^P0206 has small body phenotype, suppressible by foxo²¹ (Colombani et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^P0206 has cell growth defective phenotype, suppressible by foxo²¹ (Colombani et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Akt1¹/Akt1³ (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^en-e16E has environmental stress response defective phenotype, suppressible \| partially by Rheb^PΔ2/Rheb^PΔ1 (Saucedo et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^en-e16E has environmental stress response defective phenotype, suppressible \| partially by Rheb^PΔ2/Rheb[+] (Saucedo et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^en-e16E has environmental stress response defective phenotype, suppressible \| partially by Rheb^PΔ1/Rheb[+] (Saucedo et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^nub-AC-62 has hyperplastic phenotype, suppressible by rictor^Δ2 (Hietakangas and Cohen, 2007)
NOT suppressed by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by S6k^Scer\UAS.cMa, Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by S6k^l-1 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB has cell death decrease phenotype, non-suppressible by foxo²⁵/foxo[+] (Liu and Lehmann, 2006) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB has cell death decrease phenotype, non-suppressible by foxo²⁵/foxo²⁵ (Liu and Lehmann, 2006)
Enhancer of
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, Tor^{WT.Scer\UAS.T:Zzzz\FLAG} (Hennig and Neufeld, 2002)
NOT Enhancer of
Statement Reference Scer\GAL4^Bx-MS1096/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-enhancer of increased cell size \| somatic clone phenotype of Pten^dj189 (Gao et al., 2000)
Suppressor of
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, foxo^Scer\UAS.cKa (Kramer et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor \| partially of visible phenotype of Mmus\Foxo1^AA.Scer\UAS, Scer\GAL4^GMR.PF (Kramer et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Ddc.PL is a suppressor of cell death increase phenotype of DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^Ddc.PL (Yang et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor of cell death increase phenotype of DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF (Yang et al., 2005)
NOT Suppressor of
Statement Reference Scer\GAL4^mf9/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-suppressor of male sterile phenotype of Df(2L)Prl/prd⁴, prd^mf9 (Xue and Noll, 2002) Scer\GAL4^Bx-MS1096/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-suppressor of increased cell size \| somatic clone phenotype of Pten^dj189 (Gao et al., 2000) Scer\GAL4^mf9/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-suppressor of male sterile phenotype of Df(2L)Prl, prd⁴, prd^mf9 (Xue and Noll, 2002) Scer\GAL4^αTub84B.PL/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-suppressor of cell lethal \| somatic clone phenotype of scrib¹ (Brumby and Richardson, 2003)
Phenotype Manifest In
Enhanced by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Gao et al., 2000) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^ptc-559.1 has 1st posterior cell phenotype, enhanceable by Pk61C^EP3553, Scer\GAL4^ptc-559.1 (Cho et al., 2001) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Akt1^Scer\UAS.cRa, Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Akt1^Scer\UAS.cRa, Scer\GAL4^GMR.PF (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^fkh.PH has larval salivary gland phenotype, enhanceable by Nc^I24/Nc^I29 (Berry and Baehrecke, 2007) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^fkh.PH has larval salivary gland phenotype, enhanceable by BacA\p35^Scer\UAS.cHa, Scer\GAL4^fkh.PH (Berry and Baehrecke, 2007)
NOT Enhanced by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 has wing phenotype, non-enhanceable by S6k^Scer\UAS.cMa, Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by S6k^Scer\UAS.cMa, Scer\GAL4^GMR.PF (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by S6k^l-1 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has ommatidium phenotype, non-enhanceable by S6k^l-1 (Radimerski et al., 2002)
Suppressed by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Akt1¹/Akt1³ (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Akt1¹/Akt1³ (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^nub-AC-62 has larval salivary gland phenotype, suppressible by rictor^Δ2 (Hietakangas and Cohen, 2007) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^fkh.PH has larval salivary gland phenotype, suppressible by Tor^TED.Scer\UAS, Scer\GAL4^fkh.PH (Berry and Baehrecke, 2007)
NOT suppressed by
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 has wing phenotype, non-suppressible by S6k^Scer\UAS.cMa, Scer\GAL4^Bx-MS1096 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by S6k^l-1 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by S6k^l-1 (Radimerski et al., 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB has larval salivary gland phenotype, non-suppressible by foxo²⁵/foxo[+] (Liu and Lehmann, 2006) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB has larval salivary gland phenotype, non-suppressible by foxo²⁵/foxo²⁵ (Liu and Lehmann, 2006)
Enhancer of
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, Tor^{WT.Scer\UAS.T:Zzzz\FLAG} (Hennig and Neufeld, 2002) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATX1^82Q.Scer\UAS, Scer\GAL4^GMR.PF (Chen et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATX1^82Q.Scer\UAS, Scer\GAL4^GMR.PF (Chen et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is an enhancer of rhabdomere phenotype of Hsap\ATX1^82Q.Scer\UAS, Scer\GAL4^GMR.PF (Chen et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^fkh.PH is an enhancer of larval salivary gland phenotype of BacA\p35^Scer\UAS.cHa, Scer\GAL4^fkh.PH (Berry and Baehrecke, 2007)
NOT Enhancer of
Statement Reference Scer\GAL4^Bx-MS1096/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-enhancer of wing margin bristle phenotype of Pten^dj189 (Gao et al., 2000)
Suppressor of
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor \| partially of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, foxo^Scer\UAS.cKa (Kramer et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^Scer\UAS.cKa (Kramer et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Pten^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Gao et al., 2000) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor \| partially of interommatidial bristle phenotype of Mmus\Foxo1^AA.Scer\UAS, Scer\GAL4^GMR.PF (Kramer et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor \| partially of ommatidium phenotype of Mmus\Foxo1^AA.Scer\UAS, Scer\GAL4^GMR.PF (Kramer et al., 2003) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Ddc.PL is a suppressor of dopamine neuron phenotype of DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^Ddc.PL (Yang et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor of eye photoreceptor cell phenotype of DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF (Yang et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF (Yang et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF (Yang et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Pten^Scer\UAS.cHa, Scer\GAL4^ey.PH (Huang et al., 1999) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^ey.PH is a suppressor of ommatidium phenotype of Pten^Scer\UAS.cHa, Scer\GAL4^ey.PH (Huang et al., 1999) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^ey.PH is a suppressor of eye disc phenotype of Pten^Scer\UAS.cHa, Scer\GAL4^ey.PH (Huang et al., 1999)
NOT Suppressor of
Statement Reference Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF (Yang et al., 2005) Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF is a non-suppressor of eye photoreceptor cell phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF (Yang et al., 2005) Scer\GAL4^mf9/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-suppressor of male accessory gland phenotype of Df(2L)Prl/prd⁴, prd^mf9 (Xue and Noll, 2002) Scer\GAL4^mf9/Pi3K92E^{Scer\UAS.T:Hsap\MYC} is a non-suppressor of male accessory gland phenotype of Df(2L)Prl, prd⁴, prd^mf9 (Xue and Noll, 2002)
Additional Comments
Genetic Interactions
Statement Reference Suppresses the eye phenotype caused by Pten^Scer\UAS.cHa, when co-expressed using Scer\GAL4^ey.PH. (Huang et al., 1999) Flies expressing Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} and Pten^Scer\UAS.cGb under the control of Scer\GAL4^Bx-MS1096 have wings even smaller than flies expressing Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} or Pten^Scer\UAS.cGb alone. (Gao et al., 2000) Coexpression of Tor^{WT.Scer\UAS.T:Zzzz\FLAG} and Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in a severe reduction in wing size. (Hennig and Neufeld, 2002) Co-expression of S6k^Scer\UAS.cMa does not result in any further increase in eye size in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF. Co-expression of Akt1^Scer\UAS.cRa results in a clear increase the size of the eye and individual ommatidia in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF. Co-expression of S6k^Scer\UAS.cMa does not modify the bent wing phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096. Co-expression of Akt1^Scer\UAS.cRa in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 causes the wing to bend strongly downwards. The enlarged eye and ommatidia phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is not affected if the flies are also homozygous for S6k^l-1. The enlarged eye and ommatidia phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is completely suppressed in a Akt1¹/Akt1³ background. (Radimerski et al., 2002) Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} almost completely rescues the eye phenotype caused by expression of foxo^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF; the ommatidia and nearly all of the mechanosensory bristles are restored. Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} partially rescues the eye phenotype caused by expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF; a lack of ommatidia and mechanosensory bristles is still seen. (Kramer et al., 2003) The hyper-sensitivity of Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^en-e16E animals to starvation is partially suppressed by Rheb^PΔ1/+ or

Allele Dmel\Pi3K92EScer\UAS.T:Hsap\MYC

Allele Dmel\Pi3K92E^{Scer\UAS.T:Hsap\MYC}