FlyBase Allele Report: Dmel\Pi3K92E[UAS.Tag:MYC]

General Information

Symbol

Dmel\Pi3K92E^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0058396

Feature type

allele

Associated gene

Dmel\Pi3K92E

Associated Insertion(s)

Carried in Construct

P{WT-Dp110}

Also Known As

UAS-dp110, UAS-PI3K, UAS-p110, UAS-dPI3K, UAS Dp110, UASDp110

Key Links

Transgenic product class

cDNA

(Leevers et al., 1996)

wild_type

(Leevers et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Leevers et al., 1996)

wild_type

(Leevers et al., 1996)

Mutagen

in vitro construct

(Leevers et al., 1996)

Progenitor genotype

Carried in construct

P{WT-Dp110}

Cytology

Description

UASt regulatory sequences drive expression of a cDNA fragment encoding the wild-type Pi3K92E coding region. The open reading frame is tagged at the N-terminal end with Tag:MYC.

(Leevers et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pi3K92E

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by DJ-1α^RNAi.UAS

(Yang et al., 2005)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Chen et al., 2003)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cheng et al., 2021)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(nec)}

(López-Arias et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell growth, with Scer\GAL4^P0206

(Colombani et al., 2005, Colombani et al., 2005)

abnormal cell growth | somatic clone, with Scer\GAL4^Act5C.PP

(de la Cova et al., 2004)

abnormal cell size, with Scer\GAL4^phtm.PO

(Mirth et al., 2005)

abnormal developmental rate, with Scer\GAL4^P0206

(Colombani et al., 2005)

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Mirth et al., 2005)

abnormal endomitotic cell cycle | larval stage | nutrition conditional, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

abnormal feeding behavior, with Scer\GAL4^Adh.PF

(Britton et al., 2002)

abnormal feeding behavior, with Scer\GAL4^NPFR.6.6

(Wu et al., 2005)

abnormal locomotor behavior, with Scer\GAL4^ccb-796

(Martin-Pena et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^ccb-796

(Martin-Pena et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^Toll-6-D42

(Martin-Pena et al., 2006)

abnormal neurophysiology, with Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

abnormal neurophysiology, with Scer\GAL4^Toll-6-D42

(Martin-Pena et al., 2006)

abnormal planar polarity | somatic clone, with Scer\GAL4^Act5C.PP

(Bateman and McNeill, 2004)

abnormal size, with Scer\GAL4^en-e16E

(Wang et al., 2008)

abnormal size | adult stage, with Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

abnormal starvation stress response | larval stage | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Cinnamon et al., 2016)

abnormal stress response, with Scer\GAL4^en-e16E

(Saucedo et al., 2003)

decreased body size, with Scer\GAL4^P0206

(Colombani et al., 2005)

decreased body size | pharate adult stage, with Scer\GAL4^P0206

(Mirth et al., 2005)

decreased body size | second instar larval stage, with Scer\GAL4^phtm.PO

(Mirth et al., 2005)

decreased body size | third instar larval stage, with Scer\GAL4^phtm.PO

(Mirth et al., 2005)

decreased cell death, with Scer\GAL4^hs.PB

(Liu and Lehmann, 2006)

decreased cell number, with Scer\GAL4^P0206

(Colombani et al., 2005)

decreased cell number | somatic clone | spermatogenesis, with Scer\GAL4^NP1624

(Yuen et al., 2021)

decreased cell size, with Scer\GAL4^P0206

(Colombani et al., 2005)

fertile, with Scer\GAL4^Adh.PF

(Britton et al., 2002)

fertile, with Scer\GAL4^en-e16E

(Britton et al., 2002)

hyperplasia, with Scer\GAL4^nub-AC-62

(Hietakangas and Cohen, 2007)

increased cell size, with Scer\GAL4^AB1

(Georgiev et al., 2010)

increased cell size, with Scer\GAL4^Bx-MS1096

(Gao et al., 2000)

increased cell size | larval stage, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

increased cell size | larval stage | somatic clone | nutrition conditional, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Cinnamon et al., 2016)

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

(Bateman and McNeill, 2004, Britton et al., 2002, Hipfner et al., 2002, Prober and Edgar, 2002)

increased size | third instar larval stage, with Scer\GAL4^en.PU

(Yuen et al., 2021)

lethal, with Scer\GAL4^Act5C.PP

(Britton et al., 2002)

partially lethal - majority die, with Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

photoperiod response variant, with Scer\GAL4^phtm.PO

(Mirth et al., 2005)

short lived, with Scer\GAL4^da.PU

(Ejeskär et al., 2015)

some die during larval stage, with Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

some die during pupal stage, with Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

viable, with Scer\GAL4^Adh.PF

(Britton et al., 2002)

viable, with Scer\GAL4^en-e16E

(Britton et al., 2002)

visible, with Scer\GAL4^ap-md544

(Radimerski et al., 2002)

visible, with Scer\GAL4^Bx-MS1096

(Porstmann et al., 2008, MacDougall et al., 2004, Hennig and Neufeld, 2002, Radimerski et al., 2002)

visible, with Scer\GAL4^dpp.blk1

(Miron et al., 2001)

visible, with Scer\GAL4^en-e16E

(Wang et al., 2008)

visible, with Scer\GAL4^GMR.PF

(Buszard et al., 2013, Radimerski et al., 2002)

visible, with Scer\GAL4^GMR.PU

(Aron et al., 2010)

visible | adult stage, with Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^Tab2-201Y

(Acebes et al., 2012)

antennal lobe glomerulus DM2, with Scer\GAL4^GH298

(Acebes et al., 2012)

autophagosome, with Scer\GAL4^Lsp2.PH

(Rusten et al., 2004)

bouton | increased number, with Scer\GAL4^ccb-796

(Martin-Pena et al., 2006)

cell surface & nurse cell | somatic clone, with Scer\GAL4^Act5C.PP

(Vereshchagina and Wilson, 2006)

dendrite, with Scer\GAL4^ccb-796

(Martin-Pena et al., 2006)

ellipsoid body, with Scer\GAL4^ccb-796

(Martin-Pena et al., 2006)

embryonic/larval epidermis | somatic clone, with Scer\GAL4^Act5C.PP

(Britton et al., 2002)

embryonic/larval fat body | larval stage | somatic clone | nutrition conditional, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Cinnamon et al., 2016)

embryonic/larval fat body | somatic clone, with Scer\GAL4^Act5C.PP

(Britton et al., 2002)

embryonic/larval Malpighian tubule | somatic clone, with Scer\GAL4^Act5C.PP

(Britton et al., 2002)

embryonic/larval neuromuscular junction, with Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

embryonic/larval oenocyte | larval stage, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

embryonic/larval salivary gland, with Scer\GAL4^29BD

(Takeo et al., 2005)

embryonic/larval salivary gland, with Scer\GAL4^AB1

(Georgiev et al., 2010)

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Berry and Baehrecke, 2007)

embryonic/larval salivary gland, with Scer\GAL4^hs.PB

(Liu and Lehmann, 2006)

embryonic/larval salivary gland, with Scer\GAL4^nub-AC-62

(Hietakangas and Cohen, 2007)

eye, with Scer\GAL4^GMR.PF

(Buszard et al., 2013, Chen et al., 2003, Radimerski et al., 2002, Leevers et al., 1996)

eye, with Scer\GAL4^GMR.PU

(Aron et al., 2010)

eye, with Scer\GAL4^P0206

(Colombani et al., 2005)

eye disc | somatic clone, with Scer\GAL4^Act5C.PP

(Bateman and McNeill, 2004)

fat body, with Scer\GAL4^Act5C.PP

(Scott et al., 2004)

first posterior wing cell, with Scer\GAL4^dpp.blk1

(Miron et al., 2001)

first posterior wing cell, with Scer\GAL4^ptc-559.1

(Cho et al., 2001)

imaginal disc & cell, with Scer\GAL4^Act5C.PP

(Weinkove et al., 1999)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Leevers et al., 1996)

intestinal stem cell | adult stage, with Scer\GAL4^NP0677

(Foronda et al., 2014)

larval digestive system | somatic clone, with Scer\GAL4^Act5C.PP

(Britton et al., 2002)

lysosome, with Scer\GAL4^Lsp2.PH

(Rusten et al., 2004)

mitotic cell cycle | somatic clone, with Scer\GAL4^Act5C.PP

(Prober and Edgar, 2002)

ommatidial precursor cluster | somatic clone, with Scer\GAL4^Act5C.PP

(Bateman and McNeill, 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Xu et al., 2008, Radimerski et al., 2002, Leevers et al., 1996)

prothoracic gland, with Scer\GAL4^P0206

(Colombani et al., 2005)

prothoracic gland, with Scer\GAL4^phtm.PO

(Mirth et al., 2005)

puparium cuticle, with Scer\GAL4^phtm.PO

(Mirth et al., 2005)

ring canal, with Scer\GAL4^P0206

(Colombani et al., 2005, Colombani et al., 2005)

synapse | increased number, with Scer\GAL4^ccb-796

(Martin-Pena et al., 2006)

synapse | increased number, with Scer\GAL4^Toll-6-D42

(Martin-Pena et al., 2006)

testis | somatic clone, with Scer\GAL4^NP1624

(Yuen et al., 2021)

wing, with Scer\GAL4^ap-md544

(Radimerski et al., 2002)

wing, with Scer\GAL4^Bx-MS1096

(Porstmann et al., 2008, MacDougall et al., 2004, Hennig and Neufeld, 2002, Radimerski et al., 2002, Gao et al., 2000)

wing, with Scer\GAL4^dpp.blk1

(de la Cova et al., 2004, Leevers et al., 1996)

wing, with Scer\GAL4^P0206

(Colombani et al., 2005)

wing, with Scer\GAL4^αTub84B.PC

(de la Cova et al., 2004)

wing | posterior, with Scer\GAL4^en-e16E

(Wang et al., 2008, Goberdhan et al., 1999)

wing disc, with Scer\GAL4^29BD

(Takeo et al., 2005)

wing disc posterior compartment, with Scer\GAL4^en-e16E

(Weinkove et al., 1999)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Yuen et al., 2021)

Detailed Description

Statement

Reference

The expression of Pi3K92E^UAS.Tag:MYC under the control of Scer\GAL4^en.PU results in increased growth of the posterior compartment, seen by a decreased density of cell

In testis, wild-type clones in a Scer\GAL4^NP1624>Pi3K92E^UAS.Tag:MYC background have higher occupancy than wild-type clones induced in a wild-type background.

(Yuen et al., 2021)

Somatic clones in the larval fat body expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} display increased fat body cell size and lipid droplet diameter during starvation conditions, as compared to controls.

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Desat1.PB in larvae results in increased size of oenocytes in both fed and starvation conditions, and during starvation, oenocyte nuclei undergo higher levels of endoreplication, as compared to controls.

(Cinnamon et al., 2016)

Over-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^da.PU results in a significant decrease in lifespan, compared to controls.

(Ejeskär et al., 2015)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc.PU in cells of the wing disc proper has no significant effect on the morphology or proliferation of the overlying peripodial membrane cells in third instar larvae.

(Panneton et al., 2015)

Scer\GAL4^Dl-NP0677-mediated expression increases the number of intestinal stem cells in the adult midgut.

(Foronda et al., 2014)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF results in significant eye overgrowth.

(Buszard et al., 2013)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in the mushroom bodies under the control of Scer\GAL4^Tab2-201Y causes an increase in the size of the mushroom body lobes and calyces, compared with controls. These synaptic changes do not have a detectable consequence on odour central adaptation.

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in the projection neurons, under the control of Scer\GAL4^GH146 or Scer\GAL4^{exba-krasavietz}, does not affect central or cross adaptation to EB, IAA and benzaldehyde odours.

Coincident odorant-specific DM2 glomerular size change induced by exposure to EB is not detected when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GH298.

(Acebes et al., 2012)

Scer\GAL4^elav.PLu-mediated expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} produces substantial synaptic overgrowth at the larval neuromuscular junction and increased excitatory junctional potentials. However, no significant effect is seen on axon guidance in the visual system or on phototaxis behaviour.

(Dimitroff et al., 2012)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU at 30[o]C leads to a significant increase in eye size and to a disorganised retina, compared to controls.

(Aron et al., 2010)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^AB1 results in a 186% increase in the size of the salivary gland in larvae, which is principally mediated by an increase in cell size.

(Georgiev et al., 2010)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in a 15-20% increase in the surface area of the wing.

(Porstmann et al., 2008)

No scutellar bristle phenotypes are seen when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^sca-537.4. Expression in the wing under the control of Scer\GAL4^en-e16E leads to over-growth of the posterior compartment.

(Wang et al., 2008)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF leads to an increase in ommatidia size.

(Xu et al., 2008)

The salivary glands of animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH are greatly increased in area compared to controls at 13.5 hours after puparium formation (APF). At 24 hours APF, animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH still contain salivary gland tissue, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage. The salivary glands in the mutant animals are always present at 24 hours APF, but are partly degraded. The induction of autophagy that is seen in wild-type salivary glands at 13.5 hours APF is not seen in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH.

DNA fragmentation is detected by TUNEL in salivary glands of animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH at 13.5 hours after puparium formation, as occurs in control salivary glands at this stage.

(Berry and Baehrecke, 2007)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nub-AC-62 results in tissue overgrowth in the larval salivary glands.

(Hietakangas and Cohen, 2007)

80% of pupae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PB (using a 30 minute heat shock at 9 hours after puparium formation) still possess intact salivary glands at 20 hours after puparium formation (this is approximately 6 hours after the glands are normally destroyed in wild-type animals).

(Liu and Lehmann, 2006)

Larval expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^D42 results in twice as many synapses as wild-type.

Spontaneous release from motor neurons overexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^D42 is increased by approximately 40% compared to controls in muscle fibers F6 and F7. The mean amplitude of spontaneous release in these neurons is also increased by 40% with respect to controls. The evoked release, measured as stimulus-induced EPSPs, also produces a 22% increase in motor neurons overexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC}.

Overexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in R1-3, R4m and R4d cholinergic neurons under the control of Scer\GAL4⁷⁹⁶ results in a 56% increase in the size of the ellipsoid body. The synaptic domain of Pi3K92E-expressing neurons is, however, more than threefold larger, a much larger increase compared with the change in cell size. Pi3K92E^{Scer\UAS.T:Hsap\MYC} expression induces a thicker axon diameter and a wider crown of projections with more abundant synaptic boutons. The number of Scer\GAL4⁷⁹⁶ neurons is statistically similar in controls and flies overexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC}.

Flies overexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4⁷⁹⁶ display 18% higher density of cell branch profiles and small clear vesicles, compared with controls, reflecting a 24% increase in synaptic density. The estimated total number of synapses in the ellipsoid body of Pi3K92E^{Scer\UAS.T:Hsap\MYC} animals is 64% higher than controls. These flies show a normal maturation profile, albeit with values consistently higher than the sibling controls.

Animals overexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} in ellipsoid body neurons under the control of Scer\GAL4⁷⁹⁶ are active for longer periods and, consequently, walk longer distances than their sibling controls. The number of full walks is also higher in Pi3K92E^{Scer\UAS.T:Hsap\MYC} animals, while walking speed is unaffected.

When Pi3K92E^{Scer\UAS.T:Hsap\MYC} overexpression under the control of Scer\GAL4⁷⁹⁶ is blocked through Scer\GAL80^ts.αTub84B, supernumerary synapses are rapidly eliminated to give levels comparable to wild-type, indicating that high levels of Pi3K92E generated by Pi3K92E^{Scer\UAS.T:Hsap\MYC} overexpression are necessary not only for the formation of ectopic synapses but also for subsequent synapse maintenance.

Adult Pi3K92E^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4⁷⁹⁶ overexpression flies that have been raised at a permissive temperature in a Scer\GAL80^ts.αTub84B background (so Scer\GAL4⁷⁹⁶-driven expression is suppressed) and then later places at a restrictive temperature (where Scer\GAL80^ts.αTub84B expression is suppressed so Scer\GAL4⁷⁹⁶-driven expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} is enabled), achieve a two-fold increase of synaptic domain. This is a lower increase than in flies with constitutive Pi3K92E^{Scer\UAS.T:Hsap\MYC} expression, indicating a loss of neuronal plasticity.

Constitutive Pi3K92E^{Scer\UAS.T:Hsap\MYC} overexpression (under the control of Scer\GAL4⁷⁹⁶) increases dendritic tuft branching compared to controls. Pi3K92E^{Scer\UAS.T:Hsap\MYC} switch-on in 30 day old flies also increases dendritic branching in 52-day old flies.

(Martin-Pena et al., 2006)

Nurse cell clones that express Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP show a normal distribution of lipid droplets. However, 16% of egg chambers with these clones exhibit elongated cell surface lipid droplet-type structures in nurse cells at the periphery of the egg chamber, which are not seen in wild-type egg chambers.

(Vereshchagina and Wilson, 2006)

When Pi3K92E^{Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^Aug21 no effect on adult size is observed. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in the ring gland using Scer\GAL4^P0206 induces autonomous growth.

(Colombani et al., 2005)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} using Scer\GAL4^P0206 induces autonomous growth in the ring gland, especially in the prothoracic gland. The whole adults show reduced growth at all stages of development and emerging adults with reduced size and body weight. This decrease was attributable to an decrease in cell number in the wing and a decrease in cell number in eye. The timing of embryonic and larval development in these mutants is comparable to controls. The duration of pupal development is delayed by less than four hours.

(Colombani et al., 2005)

Overexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the regulation of Scer\GAL4^dpp.blk1 does not appreciably alter nucleolar size.

(Grewal et al., 2005)

Scer\GAL4^GMR.long>Pi3K92E^{Scer\UAS.T:Hsap\MYC} mutants show no defects in opsin regulation.

(Mikeladze-Dvali et al., 2005)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in the prothoracic gland, driven by Scer\GAL4^phm.PO, causes enlarged prothoracic gland cells during all feeding stages. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} pharate adults are 73% (females) and 80% (males) the weight of controls. This phenotype is enhanced when larvae are reared under constant light (LL) as pharate adults are then 51% the weight of controls and pupariate ~4 hours earlier than controls reared under 14 hours light:10 hours dark. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} flies are the same size as controls at hatching, but grow more slowly than controls during L1 and L2. However, when Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae are reared under LL on a yeast-supplemented diet they grow at the same rate as controls. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae overestimate their size so that they initiate metamorphosis before reaching the minimal viable weight necessary to survive pupation; 50% of Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae are able to pupariate after starvation at 2.6 hr after L3 ecdysis (AL3E) when they are only 0.36mg, instead of pupariating at 11.4 hours at a weight of 0.86mg like controls. While control larvae do not pupariate under starvation conditions unless they have been fed for at least 10 hours AL3E, Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} larvae can pupariate at 0 hour starvation. This results in the formation of partial puparia that are tanned but have failed to contract properly, although they are competent to undergo some of the earliest metamorphic processes such as eversion and elongation. However, such puparia are non-viable and any larvae that fail to pupariate at this time die. When Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} are starved after 4 hours AL3E, 73% form normal puparia, 13% form partial puparia, and the remaining larvae die. These animals exhibit molting defects in the prepupal stages. Scer\GAL4^phm.PO>Pi3K92E^{Scer\UAS.T:Hsap\MYC} flies starved at 8 hours AL3E form normal puparia and do not exhibit molting defects. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, under the control of either Scer\GAL4^P0206 or Scer\GAL4^Aug21, does not affect body size. However, Scer\GAL4^P0206>Pi3K92E^{Scer\UAS.T:Hsap\MYC} pharate adults reared under constant light (LL) are 80% the size of control pharate adults grown under the same conditions.

(Mirth et al., 2005)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^29BD, causes overgrown salivary glands and wing discs in third instar larvae.

(Takeo et al., 2005)

Fasted third instar larvae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^NPFR1.6.6 display an attenuated feeding response to solid food. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls.

(Wu et al., 2005)

Flies overexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} in dopaminergic neurons (through Scer\GAL4^ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type.

(Yang, 2005)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in clones in the eye disc results in enlarged cells with rotation defects. Precocious differentiation of cells in the eye disc is also seen.

(Bateman and McNeill, 2004)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in larger wings than normal.

(MacDougall et al., 2004)

When expression is governed by Scer\GAL4^Lsp2.PH there is a noticeable lack of large autolysosomes. In state similar to when ecdysone signalling is blocked the cytoplasm contains small autophagosomes and autolysosomes. Glycogen accumulates, probably due to a failure of autophagy to degrade glycogen. Autophagic area decreased compared to wild type.

(Rusten et al., 2004)

Expression driven by Scer\GAL4^Act5C.PP in clones in the fat body suppresses starvation-induced autophagy, but not the autophagy induced by Tor^ΔP loss of function.

(Scott et al., 2004)

Somatic clones expressing dm^Scer\UAS.cZa (driven by Scer\GAL4^αTub84B.PC, in the wing disc) are significantly larger than controls. Sibling clones next to these clones are similar in size to controls elsewhere. When Pi3K92E^{Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^dpp.blk1 or Scer\GAL4^αTub84B.PC lead to wings that are significantly larger than controls.

(de la Cova et al., 2004)

Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PF leads to enlarged eyes.

(Chen et al., 2003)

Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^HLHmδ has no effect on ommatidial rotation.

(Gaengel and Mlodzik, 2003)

Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^en-e16E animals are hyper-sensitive to starvation.

(Saucedo et al., 2003)

Caspase activation in cells posterior to the furrow in eye discs is unaffected by Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^GMR.PF.

(Yang and Baker, 2003)

Clones of cells expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in the gut, fat body, Malpighian tubules and epidermis are only slightly larger than control cells at the time of larval hatching. After several days of starvation on 20% sucrose, these cells are much larger than adjacent control cells and have a visibly increased DNA content. Gut and fat body cells expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP continue to replicate their DNA for at least 2-3 days under starvation conditions. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} in third larval instar fat body cells under the control of Scer\GAL4^Act5C.PP increases the opacity of the cytoplasm, thus promoting nutrient storage. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Adh.PF in fed animals does not affect survival. In contrast, expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Adh.PF in starved animals results in first instar larvae dying within 3-4 days of hatching (control animals survive 8-9 days). Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en-e16E in starved animals results in them dying within 2-3 days, 4-5 days before controls. Animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Adh.PF feed poorly, often having no food in their gut, and frequently wander away from their food.

(Britton et al., 2002)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in a slight increase in wing size and in males the wings show a downward curvature.

(Hennig and Neufeld, 2002)

Clones of cells expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP are 28% larger than control cells.

(Hipfner et al., 2002)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in wing disc cells results in an increase in cell size compared to controls. The proportion of cells in G₁ is decreased compared to controls. Clones expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP are not visibly round, do not generate ectopic folds and do not bulge out of the epithelium.

(Prober and Edgar, 2002)

Animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF have a significantly larger eye than normal, due to an increase in ommatidia size. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ap-md544 results in dramatic overgrowth of the wing, inhibiting wing unfolding. Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 causes the wing to bend downwards slightly.

(Radimerski et al., 2002)

When Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^ptc-559.1 results in a increase in the distance between the L3 and L4 veins in the wing.

(Cho et al., 2001)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1 results in enlargement of the region encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin.

(Miron et al., 2001)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^Bx-MS1096 results in an increased wing size.

(Gao et al., 2000)

When expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^en-e16E most animals die as larvae or pupae with the few survivors having distorted or crumpled wings. The posterior compartment of these wings is greatly enlarged relative to the anterior compartment.

(Goberdhan et al., 1999)

When expression is driven in imaginal disc cells by Scer\GAL4^Act5C.PP the size of the cells is increased (as measured by FSC (forward scatter)). Cells are larger during G1 and the S+G2 phase. Overexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} is not sufficient to increase cell number. When expression is driven by Scer\GAL4^en-e16E, the size of the posterior compartment of the wing disc is significantly increased. There is no comparable effect on the anterior compartment.

(Weinkove et al., 1999)

Scer\GAL4^dpp.blk1 mediated expression caused expansion of the wing margin due to an increase in overall number of cells and their size. Scer\GAL4^GMR.PF mediated expression causes enlarged and bulging roughened eyes with fused ommatidia and misplaced or duplicated bristles.

(Leevers et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 has visible phenotype, enhanceable by Akt^UAS.cRa, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Akt^UAS.cRa, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

NOT Enhanced by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by Ptp61F^{UAS.n.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 has visible phenotype, non-enhanceable by S6k^UAS.cMa, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by S6k^l-1, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by S6k^UAS.cMa, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Suppressed by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^AB1 has increased cell size phenotype, suppressible by Trpm²⁸

(Georgiev et al., 2010)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^nub-AC-62 has hyperplasia phenotype, suppressible by rictor^Δ2

(Hietakangas and Cohen, 2007)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206 has decreased cell number phenotype, suppressible by foxo²¹

(Colombani et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206 has decreased cell size phenotype, suppressible by foxo²¹

(Colombani et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206 has decreased body size phenotype, suppressible by foxo²¹

(Colombani et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^P0206 has abnormal cell growth phenotype, suppressible by foxo²¹

(Colombani et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E has abnormal stress response phenotype, suppressible | partially by Rheb^PΔ2/Rheb^PΔ1

(Saucedo et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E has abnormal stress response phenotype, suppressible | partially by Rheb^PΔ2/Rheb[+]

(Saucedo et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E has abnormal stress response phenotype, suppressible | partially by Rheb^PΔ1/Rheb[+]

(Saucedo et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Akt¹/Akt³

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E has partially lethal - majority die phenotype, suppressible | partially by Pten^UAS.cGa, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E has some die during pupal stage phenotype, suppressible | partially by Pten^UAS.cGa, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E has some die during larval stage phenotype, suppressible | partially by Pten^UAS.cGa, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

NOT suppressed by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Ptp61F^{UAS.n.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by dj-1β^Δ93/dj-1β^Δ93

(Aron et al., 2010)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB has decreased cell death phenotype, non-suppressible by foxo²⁵/foxo[+]

(Liu and Lehmann, 2006)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB has decreased cell death phenotype, non-suppressible by foxo²⁵/foxo²⁵

(Liu and Lehmann, 2006)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by S6k^l-1

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by S6k^UAS.cMa, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Enhancer of

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PF

(Kim et al., 2010)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^Bx-MS1096, p53^EP-1

(Lee et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, mTor^{WT.UAS.Tag:FLAG}

(Hennig and Neufeld, 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^Bx-MS1096, mTor^{WT.UAS.Tag:FLAG}

(Hennig and Neufeld, 2002)

NOT Enhancer of

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA is a non-enhancer of increased occurrence of cell division | larval stage phenotype of N^HMS00001, Scer\GAL4^wor.PA

(Sood et al., 2022)

Scer\GAL4^Bx-MS1096/Pi3K92E^UAS.Tag:MYC is a non-enhancer of increased cell size | somatic clone phenotype of Pten^dj189

(Gao et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^C587/Pi3K92E^UAS.Tag:MYC is a suppressor of decreased cell number | somatic clone | spermatogenesis phenotype of Pten^dj189

(Yuen et al., 2021)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^da.PU is a suppressor of partially lethal - majority die phenotype of Pi3K21B^UAS.Tag:HA, Scer\GAL4^da.PU

(Ejeskär et al., 2015)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^da.PU is a suppressor of decreased body size | larval stage phenotype of Pi3K21B^UAS.Tag:HA, Scer\GAL4^da.PU

(Ejeskär et al., 2015)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Ddc.PL is a suppressor of increased cell death phenotype of DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E is a suppressor | partially of partially lethal - majority die phenotype of Pten^UAS.cGa, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^ey.PH is a suppressor of abnormal size | adult stage phenotype of Pten^UAS.cHa, Scer\GAL4^ey.PH

(Huang et al., 1999)

NOT Suppressor of

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA is a non-suppressor of increased occurrence of cell division | larval stage phenotype of N^HMS00001, Scer\GAL4^wor.PA

(Sood et al., 2022)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^da.PU is a non-suppressor of abnormal developmental rate | larval stage phenotype of Pi3K21B^UAS.Tag:HA, Scer\GAL4^da.PU

(Ejeskär et al., 2015)

Scer\GAL4^GMR.PF/Pi3K92E^UAS.Tag:MYC is a non-suppressor of visible phenotype of Ras85D^KP

(Wu et al., 2009)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^sca-537.4 is a non-suppressor of visible phenotype of Hsap\TGIF2LX^UAS.Tag:MYC, Scer\GAL4^sca-537.4

(Wang et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E is a non-suppressor of visible phenotype of Hsap\TGIF2LX^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Wang et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E is a non-suppressor of abnormal size phenotype of Hsap\TGIF2LX^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Wang et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E is a non-suppressor of decreased cell size | adult stage phenotype of Hsap\TGIF2LX^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Wang et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E is a non-suppressor of decreased cell number | adult stage phenotype of Hsap\TGIF2LX^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Wang et al., 2008)

Scer\GAL4^αTub84B.PL/Pi3K92E^UAS.Tag:MYC is a non-suppressor of FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype of scrib¹

(Brumby and Richardson, 2003)

Scer\GAL4^mf9/Pi3K92E^UAS.Tag:MYC is a non-suppressor of male sterile phenotype of Df(2L)Prl/prd⁴, prd^mf9

(Xue and Noll, 2002)

Scer\GAL4^mf9/Pi3K92E^UAS.Tag:MYC is a non-suppressor of male sterile phenotype of Df(2L)Prl, prd⁴, prd^mf9

(Xue and Noll, 2002)

Scer\GAL4^Bx-MS1096/Pi3K92E^UAS.Tag:MYC is a non-suppressor of increased cell size | somatic clone phenotype of Pten^dj189

(Gao et al., 2000)

Other

Statement

Reference

Pi3K92E^UAS.Tag:MYC, SREBP^UAS.Exel, Scer\GAL4^Bx-MS1096 has visible phenotype

(Porstmann et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^fkh.PH has embryonic/larval salivary gland phenotype, enhanceable by Dronc^I24/Dronc^I29

(Berry and Baehrecke, 2007)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^fkh.PH has embryonic/larval salivary gland phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^fkh.PH

(Berry and Baehrecke, 2007)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Akt^UAS.cRa, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Akt^UAS.cRa, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^ptc-559.1 has first posterior wing cell phenotype, enhanceable by Pdk1^EP3553, Scer\GAL4^ptc-559.1

(Cho et al., 2001)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Pten^UAS.cGb, Scer\GAL4^Bx-MS1096

(Gao et al., 2000)

NOT Enhanced by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Ptp61F^{UAS.n.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 has wing phenotype, non-enhanceable by S6k^UAS.cMa, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by S6k^UAS.cMa, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by S6k^l-1

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, non-enhanceable by S6k^l-1

(Radimerski et al., 2002)

Suppressed by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^AB1 has embryonic/larval salivary gland phenotype, suppressible by Trpm²⁸

(Georgiev et al., 2010)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by RpL8^RNAi.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by Hsap\RPL8^DN.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^fkh.PH has embryonic/larval salivary gland phenotype, suppressible by mTor^TED.UAS, Scer\GAL4^fkh.PH

(Berry and Baehrecke, 2007)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^nub-AC-62 has embryonic/larval salivary gland phenotype, suppressible by rictor^Δ2

(Hietakangas and Cohen, 2007)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Akt¹/Akt³

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Akt¹/Akt³

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E has wing | posterior phenotype, suppressible by Pten^UAS.cGa, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

NOT suppressed by

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Ptp61F^{UAS.n.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by dj-1β^Δ93/dj-1β^Δ93

(Aron et al., 2010)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB has embryonic/larval salivary gland phenotype, non-suppressible by foxo²⁵/foxo[+]

(Liu and Lehmann, 2006)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB has embryonic/larval salivary gland phenotype, non-suppressible by foxo²⁵/foxo²⁵

(Liu and Lehmann, 2006)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 has wing phenotype, non-suppressible by S6k^UAS.cMa, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by S6k^l-1

(Radimerski et al., 2002)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by S6k^l-1

(Radimerski et al., 2002)

Enhancer of

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PF

(Kim et al., 2010)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^fkh.PH is an enhancer of embryonic/larval salivary gland phenotype of BacA\p35^UAS.cHa, Scer\GAL4^fkh.PH

(Berry and Baehrecke, 2007)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is an enhancer of rhabdomere phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, mTor^{WT.UAS.Tag:FLAG}

(Hennig and Neufeld, 2002)

NOT Enhancer of

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA is a non-enhancer of larval neuroblast phenotype of N^HMS00001, Scer\GAL4^wor.PA

(Sood et al., 2022)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA is a non-enhancer of larval central brain phenotype of N^HMS00001, Scer\GAL4^wor.PA

(Sood et al., 2022)

Scer\GAL4^Bx-MS1096/Pi3K92E^UAS.Tag:MYC is a non-enhancer of wing margin bristle phenotype of Pten^dj189

(Gao et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^C587/Pi3K92E^UAS.Tag:MYC is a suppressor of cyst progenitor cell | somatic clone phenotype of Pten^dj189

(Yuen et al., 2021)

Scer\GAL4^C587/Pi3K92E^UAS.Tag:MYC is a suppressor of cyst cell of testis | somatic clone phenotype of Pten^dj189

(Yuen et al., 2021)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic neuron phenotype of DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of eye photoreceptor cell phenotype of DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor | partially of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKa

(Kramer et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor | partially of interommatidial bristle phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor | partially of ommatidium phenotype of Mmus\Foxo1^AA.UAS, Scer\GAL4^GMR.PF

(Kramer et al., 2003)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Pten^UAS.cGb, Scer\GAL4^Bx-MS1096

(Gao et al., 2000)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E is a suppressor of wing | posterior phenotype of Pten^UAS.cGa, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Pten^UAS.cHa, Scer\GAL4^ey.PH

(Huang et al., 1999)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^ey.PH is a suppressor of ommatidium phenotype of Pten^UAS.cHa, Scer\GAL4^ey.PH

(Huang et al., 1999)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^ey.PH is a suppressor of eye disc phenotype of Pten^UAS.cHa, Scer\GAL4^ey.PH

(Huang et al., 1999)

NOT Suppressor of

Statement

Reference

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA is a non-suppressor of larval neuroblast phenotype of N^HMS00001, Scer\GAL4^wor.PA

(Sood et al., 2022)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA is a non-suppressor of larval central brain phenotype of N^HMS00001, Scer\GAL4^wor.PA

(Sood et al., 2022)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^da.PU is a non-suppressor of embryonic/larval fat body | larval stage phenotype of Pi3K21B^UAS.Tag:HA, Scer\GAL4^da.PU

(Ejeskär et al., 2015)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Lsp2.PH is a non-suppressor of embryonic/larval fat body phenotype of Acn^UAS.Tag:MYC, Scer\GAL4^Lsp2.PH

(Haberman et al., 2010)

Scer\GAL4^GMR.PF/Pi3K92E^UAS.Tag:MYC is a non-suppressor of eye phenotype of Ras85D^KP

(Wu et al., 2009)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^sca-537.4 is a non-suppressor of scutellar bristle phenotype of Hsap\TGIF2LX^UAS.Tag:MYC, Scer\GAL4^sca-537.4

(Wang et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E is a non-suppressor of wing | posterior phenotype of Hsap\TGIF2LX^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Wang et al., 2008)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a non-suppressor of eye photoreceptor cell phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Scer\GAL4^mf9/Pi3K92E^UAS.Tag:MYC is a non-suppressor of male accessory gland phenotype of Df(2L)Prl/prd⁴, prd^mf9

(Xue and Noll, 2002)

Scer\GAL4^mf9/Pi3K92E^UAS.Tag:MYC is a non-suppressor of male accessory gland phenotype of Df(2L)Prl, prd⁴, prd^mf9

(Xue and Noll, 2002)

Other

Statement

Reference

Df(3L)H99/Df(3L)XR38, Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA has adult mushroom body phenotype

(Siegrist et al., 2010)

Df(3L)H99/Df(3L)XR38, Pi3K92E^UAS.Tag:MYC, Scer\GAL4^wor.PA has neuroblast | ectopic phenotype

(Siegrist et al., 2010)

Pi3K92E^UAS.Tag:MYC, SREBP^UAS.Exel, Scer\GAL4^Bx-MS1096 has wing phenotype

(Porstmann et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} suppresses embryonic lethality seen with expression of Pi3K21B^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^da.PU. Co-expression of Pi3K21B^{Scer\UAS.T:Ivir\HA1} suppresses the small body phenotype in larvae with expression of Pi3K21B^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^da.PU, but larvae remain first instars for 2 weeks until they die and retain fat body abnormalities.

(Ejeskär et al., 2015)

Co-expression of Ptp61F^{Scer\UAS.n.T:Hsap\MYC} has no overt effect on the eye overgrowth phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF.

(Buszard et al., 2013)

dj-1β^Δ93/dj-1β^Δ93 does not suppress the eye phenotypes (including increased eye size) in flies with expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU at 30[o]C.

(Aron et al., 2010)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^AB1 in a trpm²⁸ background does not result in an increase in cell size in the larval salivary gland.

(Georgiev et al., 2010)

Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} does not suppress the ability of Acn^{Scer\UAS.T:Hsap\MYC} expressed under the control of Scer\GAL4^Lsp2.PH to induce autolysosome formation in the fat body in the absence of starvation.

(Haberman et al., 2010)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} enhances the rough eye phenotype seen when Dcp-1^Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4^GMR.PF.

(Kim et al., 2010)

Neuroblasts are detected in the mushroom body of 2 week old adults expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^wor.PA in a Df(3L)H99/Df(3L)XR38 background (mushroom body neuroblasts are not seen in wild-type adults).

(Siegrist et al., 2010)

The eye phenotype in Ras85D^KP mutant flies is not suppressed by ectopic expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF.

(Wu et al., 2009)

Co-expression of HLH106^{Scer\UAS.Exel} and Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in flies with severely deformed wings.

(Porstmann et al., 2008)

Expression of RpL8^{dsRNA.Scer\UAS} partially suppresses the increase in ommatidia size seen when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

Co-expression of Tor^TED.Scer\UAS suppresses the salivary gland overgrowth phenotype that is seen in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^fkh.PH and at 24 hours after puparium formation the salivary glands are predominantly degraded in the double mutant animals.

The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^fkh.PH is significantly enhanced if the animals are also carrying Nc^I24/Nc^I29.

(Berry and Baehrecke, 2007)

The overgrowth of the salivary gland that is seen larvae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nub-AC-62 is suppressed by rictor^Δ2.

(Hietakangas and Cohen, 2007)

The persistence of salivary glands seen in pupae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PB (using a 30 minute heat shock at 9 hours after puparium formation) is still seen if the animals are also heterozygous or homozygous for foxo²⁵.

(Liu and Lehmann, 2006)

Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^Lsp2.PH suppresses the reduced triglyceride phenotype see in Df(3L)melt^Δ1 homozygous embryos.

(Teleman et al., 2005)

Coexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF suppresses the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. The eyes are restored to normal size, and the organisation of the ommatidia is significantly improved.

(Yang et al., 2005)

DJ-1α^{dsRNA.Scer\UAS} flies coexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} (both in the dopaminergic neurons through Scer\GAL4^ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type.

(Yang, 2005)

Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} almost completely rescues the eye phenotype caused by expression of foxo^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF; the ommatidia and nearly all of the mechanosensory bristles are restored. Co-expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} partially rescues the eye phenotype caused by expression of Mmus\Foxo1^AA.Scer\UAS under the control of Scer\GAL4^GMR.PF; a lack of ommatidia and mechanosensory bristles is still seen.

(Kramer et al., 2003)

The hyper-sensitivity of Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^en-e16E animals to starvation is partially suppressed by Rheb^PΔ1/+ or Rheb^PΔ2/+ and more completely suppressed by Rheb^PΔ1/Rheb^PΔ2.

(Saucedo et al., 2003)

Coexpression of Tor^{WT.Scer\UAS.T:Zzzz\FLAG} and Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in a severe reduction in wing size.

(Hennig and Neufeld, 2002)

Co-expression of S6k^Scer\UAS.cMa does not result in any further increase in eye size in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF. Co-expression of Akt1^Scer\UAS.cRa results in a clear increase the size of the eye and individual ommatidia in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF. Co-expression of S6k^Scer\UAS.cMa does not modify the bent wing phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096. Co-expression of Akt1^Scer\UAS.cRa in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 causes the wing to bend strongly downwards. The enlarged eye and ommatidia phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is not affected if the flies are also homozygous for S6k^l-1. The enlarged eye and ommatidia phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is completely suppressed in a Akt1¹/Akt1³ background.

(Radimerski et al., 2002)

Flies expressing Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} and Pten^Scer\UAS.cGb under the control of Scer\GAL4^Bx-MS1096 have wings even smaller than flies expressing Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} or Pten^Scer\UAS.cGb alone.

(Gao et al., 2000)

Flies expressing both Pi3K92E^{Scer\UAS.T:Hsap\MYC} and Pten^Scer\UAS.cGa driven by Scer\GAL4^en-e16E show a much higher viability than those expressing either alone. The posterior compartment of the wing is nearly as reduced in area as in animals expressing Pten^Scer\UAS.cGa driven by Scer\GAL4^en-e16E alone.

(Goberdhan et al., 1999)

Suppresses the eye phenotype caused by Pten^Scer\UAS.cHa, when co-expressed using Scer\GAL4^ey.PH.

(Huang et al., 1999)

Xenogenetic Interactions

Statement

Reference

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} suppresses the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} enhances the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} is unable to suppress the growth phenotypes seen in the wings and bristles of flies expressing Hsap\TGIF2LX^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en-e16E or Scer\GAL4^sca-537.4 respectively.

(Wang et al., 2008)

Expression of Hsap\RPL8^DN.Scer\UAS partially suppresses the increase in ommatidia size seen when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when one of BacA\p35^Scer\UAS.cHa or Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^fkh.PH is significantly increased when both BacA\p35^Scer\UAS.cHa and Pi3K92E^{Scer\UAS.T:Hsap\MYC} are co-expressed under the control of Scer\GAL4^fkh.PH.

(Berry and Baehrecke, 2007)

Coexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} with Hsap\MAPT^{V337M.Scer\UAS}, under the control of Scer\GAL4^GMR.PF has no effect on the Hsap\MAPT^{V337M.Scer\UAS}-induced toxicity in the eye.

(Yang et al., 2005)

The addition of Pi3K92E^{Scer\UAS.T:Hsap\MYC} dramatically worsens the Hsap\ATX1^82Q.Scer\UAS (driven by Scer\GAL4^GMR.PF) neurodegeneration phenotype in the eye.

(Chen et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25914

y¹ w^*; P{WT-Dp110}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

Pi3K92E^UAS.T:Myc

Pi3K92E^UAS.Tag:MYC

UAS-dp110

(Grewal et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (83)

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