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General Information
Symbol
Dmel\Pi3K92ED954A.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0058398
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Pi3KDN, Dp110D954A, UAS-dp110D954A, UAS-Dp110DN, UAS-Dp110DN, UAS-Dp110KD, UAS-PI3KD954A, UAS-PI3KDN, PI3KD954A, Dp110DN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a Pi3K92E cDNA fragment, with a mutation (D954A) introduced the putative ATP binding site. The open reading frame is tagged at the N-terminal end with Tag:MYC.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
imaginal disc & cell, with Scer\GAL4Act5C.PP
Detailed Description
Statement
Reference
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4hh.PU results in decreased size of the posterior compartment in third instar larval wing discs.
There is an increase in the frequency of clone splitting in the Pi3K92ED954A.Scer\UAS.T:Hsap\MYC-expressing 'loser' cells when cell competition is induced in the pupal notum through generation of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC-expressing clones in wild type tissues compared with wild type clones in wild type tissue. The 'loser' clones show reduced compactness over time. The maximum speed of relaxation of junctions after laser nanoablation is reduced in loser-loser and loser-winner junctions compared with winner-winner junctions.
Knockdown of Pi3K92E through the expression of dominant-negative Pi3K92ED954A.Scer\UAS.T:Hsap\MYC, under the control of Scer\GAL4ppl.PP, leads to small cell size (approximately 50% the size of wild-type) and an ectopic lipid storage phenotype in the salivary gland.
Expression of Pi3K92EScer\UAS.T:Hsap\MYC in the mushroom bodies under the control of Scer\GAL4Tab2-201Y causes a decrease in the size of the mushroom body lobes and calyces, compared with controls. These synaptic changes do not have a detectable consequence on odour central adaptation. Expression of Pi3K92EScer\UAS.T:Hsap\MYC in the projection neurons, under the control of Scer\GAL4GH146 or Scer\GAL4exba-krasavietz, does not affect central or cross adaptation to EB, IAA and benzaldehyde odours. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in both the eLNs and iLNs, under the control of both Scer\GAL4GH298 and Scer\GAL4exba-krasavietz results in normal adaption to EB, IAA and benzaldehyde, indicating that olfactory central adaption depends on the eLN/iLN activity ratio. Coincident odorant-specific DM2 glomerular size change induced by exposure to EB is not detected when Pi3K92ED954A.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4exba-krasavietz. Under the control of both Scer\GAL4exba-krasavietz and Scer\GAL4GH298, central adaptation is restored, and likewise, the size of DM2 enlarges significantly (+24%) while that of the V glomerulus remains unaffected.
Subperineurial expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Gli-rL82 in a wild-type genetic background has only a minor effect on larval peripheral nerves.
Overexpression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in motor neurons, under the control of Scer\GAL4D42, results in decreased synaptic growth and increased neuronal excitability. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in larval muscle, using Scer\GAL4C57, results in a decrease in bouton number (by approximately 20%) and muscle size. In Scer\GAL4C57>Pi3K92ED954A.Scer\UAS.T:Hsap\MYC larvae, muscle capacitance is reduced compared to wild-type, consistent with reduced muscle size. The amplitudes of EJCs from Scer\GAL4C57>Pi3K92ED954A.Scer\UAS.T:Hsap\MYC are significantly increased, although the frequency, mean amplitude, and distribution of mEJCs are normal.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in 30-32 neurons of the antennal lobe under the control of Scer\GAL4GH298 reduces neuronal branching. There are no significant changes in cell number in these mutants. The antennal lobe displays reduced activity when exposed to benzaldehyde or isoamylacetate in mutants compared to controls. This reduction is more pronounced in the medial rather than the lateral region of the antennal lobe, for either odorant stimulation. Pi3K92ED954A.Scer\UAS.T:Hsap\MYC expressing Scer\GAL4GH298 neurons do not exhibit a preferential branching in the medial antennal lobe; therefore this activity difference may indicate a functional specialization within the branching arbor of the Scer\GAL4GH298 set of neurons. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in 30-32 neurons of the antennal lobe under the control of Scer\GAL4GH298 leads to odorant perception changes. Response to benzaldehyde, ethyl hexanoate, ethyl butyrate, and propionic acid shifts towards repulsion. The strength of repulsive reaction is proportional to the odorant concentration. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in 30-32 neurons of the antennal lobe under the control of Scer\GAL4GH298 in a Scer\GAL80Cha.3.3P background where Scer\GAL4GH298-driven expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC is repressed in cha-positive neurons leads to odorant perception changes that are the same as when Scer\GAL80Cha.3.3P is not present. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in 6-8 neurons of the antennal lobe under the control of Scer\GAL4exba-krasavietz reduces neuronal branching. There are no significant changes in cell number in these mutants. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in 6-8 neurons of the antennal lobe under the control of Scer\GAL4exba-krasavietz leads to odorant perception changes. Response to benzaldehyde, ethyl hexanoate, ethyl butyrate, and propionic acid shifts towards attraction. The strength of attractive reaction is proportional to the odorant concentration. A Scer\GAL80Cha.3.3P background completely abolishes this attraction. Inhibition of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC expression until the first day of adult life (through the presence of Scer\GAL80ts.αTub84B) does not alter odorant perception changes compared to flies with chronic Pi3K92ED954A.Scer\UAS.T:Hsap\MYC expression. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in Scer\GAL4exba-krasavietz and Scer\GAL4GH298 expressing neurons results in synapse reduction in both sets of neurons and wild-type odorant perception, independent of stimulus. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in Scer\GAL4exba-krasavietz neurons where the mushroom body component of the krasavietz domain is silenced through the presence of Scer\GAL80Mef2.PT still leads to the increase of olfactory indexes when tested for 1-Hexanol. Upregulation of Pi3K92E through expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Mef2.247 causes a substantial reduction of neural branching in its MB domain. However, odorant perception is not affected in these mutants. Silencing of krasavietz mushroom body neurons through expression of Scer\GAL80ple.PK in Pi3K92ED954A.Scer\UAS.T:Hsap\MYC/Scer\GAL4exba-krasavietz mutants does not prevent the change in olfactory perception that is characteristic of Scer\GAL4exba-krasavietz-related neurons.
Blocking Pi3K92E activity in motor neurons by expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in motor neurons under the control of Scer\GAL4D42 decreases synapse number about two-fold. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in motor neurons under the control of Scer\GAL4D42 decreases motor axon diameter.
In contrast to wild-type, transgenic flies expressing Scer\GAL4ppl.PP>Pi3K92ED954A.Scer\UAS.T:Hsap\MYC and reared on medium supplemented with Apis mellifera royal jelly do not show increased body size. However, similarly to wild-type flies in response to royal jelly, they display shortened developmental time compared with flies reared on control medium.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4amn-c651 results in a reduction in the size of the prothoracic gland in larvae compared with controls at the equivalent time point of 5 days after egg deposition. These animals show only a moderate developmental delay and pupate, eclosing as larger than normal flies. The defects in developmental rate and adult size are suppressed by feeding 20-hydroxyecdysone to larvae.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC using Scer\GAL4Bx-MS1096 results in an abnormally small wing.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC using Scer\GAL4nub-AC-62 results in abnormally small wings in which cell size and cell number are decreased.
Expression of InRK1409A.Scer\UAS under the control of Scer\GAL4da.Switch.PT, induced by RU486, increases median lifespan by 7% and maximum lifespan by 5%. Ubiquitous adult-specific induction of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT decreases female fecundity. Flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT all survive for significantly longer on food supplemented with 20mM paraquat compared to controls. Flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT survive for longer in the presence of DTT, compared to controls.
Compared with controls, the age-related dysplasia of the intestinal epithelium is strongly reduced in flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in significant lifespan shortening compared with controls. Compared with controls, female flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4bun-GSG5961 show a moderate, but significant, reduction in intestinal cell proliferation at old age. These flies are significantly longer lived when exposed to RU486 than isogenic siblings exposed to mock treatment.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4dpp.blk1 results in a 20-30% decrease in the size of the Scer\GAL4dpp.blk1-expressing wing compartment.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in the amnioserosa cells, driven by Scer\GAL4Kr.PM, results in the failure of embryos to retract the germ band.
Larval expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4D42 exhibit a reduction in synapse number compared to wild-type. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4796 does not cause a reduction in synaptic volume. However, there is both a reduction in cell size and an increase in cell number. Supernumerary neurons contribute to an almost normal synaptic domain that compensates for the smaller cell size. Animals overexpressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in ellipsoid body neurons under the control of Scer\GAL4796 are inactive for longer periods and, consequently, walk shorter distances than their sibling controls. The number of full walks is also lower in Pi3K92ED954A.Scer\UAS.T:Hsap\MYC animals, while walking speed is unaffected.
Hemocytes expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC (under the control of Scer\GAL4crq.PO) exhibit normal distribution at all stages of development, and appear morphologically indistinguishable from their wild-type counterparts. Pi3K92ED954A.Scer\UAS.T:Hsap\MYC-expressing hemocytes migrate along the ventral midline in a pattern identical to that seen in wild-type embryos, demonstrating velocity, directionality, and polarity equal to wild-type cells. Laser-ablated Pi3K92ED954A.Scer\UAS.T:Hsap\MYC-expressing embryos (under the control of Scer\GAL4crq.PO) fail to chemotax toward the wound site and the wound remains largely undetected by the hemocytes up to 1 hour after laser ablation. The same result is obtained when beads are implanted in the embryo. There is no significant different in hemocyte number between Pi3K92ED954A.Scer\UAS.T:Hsap\MYC-expressing embryos and wild-type.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4amn-c651, results in pupae and adults that are larger than wild-type controls. The wing epidermis of these flies is increased, suggesting that most of this increased size results from increased cell size rather than cell number. Expression of this transgene under the control of Scer\GAL4amn-X8 causes an increase in pupal length. Flies that express one copy of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC, under the control of one copy of either Scer\GAL4amn-c651 or Scer\GAL4amn-X8, pupariate about 36 hours after wild-type controls, which results mostly from a prolonged third instar stage. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4amn-c651 causes a reduction of prothoracic gland cell size.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC, under the control of Scer\GAL4Bx-MS1096, reduces wing size.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 results in small adult wings. This phenotype is not affected by null mutations in Pi3K92E.
When Pi3K92ED954A.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4P0206, animals exhibit increased growth: adults have 17% greater weight on average. This increase was attributable to an increase in cell number in the wing and eye. The timing of embryonic and larval development in these mutants is comparable to controls. The duration of pupal development is advanced by less than four hours. A 1 to 3 hour delay is seen in the time Pi3K92ED954A.Scer\UAS.T:Hsap\MYC (without driver) mutants enter pupal development.
When fed ad libitum, third instar larvae expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC, under the control of Scer\GAL4NPFR1.6.6 display hyperactive feeding on solid food, similar to control third instar larvae deprived of food for 40 minutes.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC, under the control of Scer\GAL4GMR.PF has no effect on eye morphology.
Inhibition of PI3K signaling in dopaminergic neurons by means of overexpression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC (through Scer\GAL4ple.PF) results in an elevation in the levels of reactive oxygen species (ROS).
Hemocytes in Pi3K92ED954A.Scer\UAS.T:Hsap\MYC; Scer\GAL4srp.Hemo late embryos do not exhibit abnormal aggregation.
Cells in sector C of the wing expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4dpp.blk1 are 12.6% smaller than control cells. The average area of the mutant sector is reduced by 18.7% compared to controls and the average number of cells in the mutant sector is reduced by 13.3%. In addition to the autonomous reduction in the area of the sector expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4dpp.blk1 (sector C), a non-autonomous reduction of all other wing sectors is seen. In the B sector of mosaic wings the reduction in area is 20%, while the global reduction in area of the wing is 7%. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ap-md544 results in a clear decrease in size and number of chaetae compared to controls. In the acrostical region there is an increase in density of microchaetae compared to wild type, which is due to both reduced cell size and fewer cells between the microchaetae than normal (4.16 in mutant tissue compared to 5.34 in wild type).
When expression is driven by Scer\GAL4Lsp2.PH, relatively late in the larval fat body, no effect on autophagic activity of fat droplet aggregation is detectable.
When expression is driven by Scer\GAL4GMR.PF eye size is reduced.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PI does not lead to increased larval wandering. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF results in the formation of relatively normal eyes with fewer and smaller cells.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PP in clones of cells does not promote cell growth or DNA endoreplication. Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Adh.PF, Scer\GAL4en-e16E or Scer\GAL4Act5C.PP has no effect on viability under starvation conditions.
The border cells in the ovaries of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC; Scer\GAL4slbo.2.6 animals form normal numbers and sizes of cellular extensions, and migrate normally.
When Pi3K92ED954A.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4ptc-559.1 results in a reduction in the distance between the L3 and L4 veins in the wing.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4slbo.2.6 has no effect on border cell migration.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4dpp.blk1 results in reduction in size of the region encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4Bx-MS1096 results in an decreased wing size.
Expression of high levels of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC in embryos results in ectopic apoptosis and extensive loss of cuticle. Ectopic expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4ey.PH results in eyes that are smaller in size than that of wild-type controls due to a dramatic decrease in cell number.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E results in a decrease in size of the posterior compartment of the wing disc.
The targeted expression of the dominant negative Pi3K92ED954A.Scer\UAS.T:Hsap\MYC by Scer\GAL4Bx-MS1096 in the developing eye or wing causes a reduction in cell size in the eye, and in both cell size and number in the wing.
When expression is driven by Scer\GAL4dpp.blk1 the area between wing veins LIII and LIV is significantly reduced due to a decrease in both cell number and cell size. When expression is driven by Scer\GAL4Bx-MS1096 the whole wing is significantly reduced in size.
When expression is driven in imaginal disc cells by Scer\GAL4Act5C.PP the size of the cells is slightly decreased (as measured by FSC (forward scatter)). Overexpression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC is sufficient to reduce the rate of increase of cell number. When expression is driven by Scer\GAL4en-e16E, the size of the posterior compartment of the wing disc is significantly reduced. There is no comparable effect on the anterior compartment.
Scer\GAL4dpp.blk1 mediated expression caused reduction of the wing margin due to a decrease in cell size. Scer\GAL4GMR.PF mediated expression causes small and flat eyes.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4da.Switch.PT has decreased fecundity | female | RU486 conditional phenotype, non-suppressible by foxoΔ94
Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4da.Switch.PT has viable | RU486 conditional phenotype, non-suppressible by foxoΔ94
Pi3K92ED954A.UAS.Tag:MYC, Scer\GAL4Bx-MS1096 has visible phenotype, non-suppressible by eIF3hk09003/eIF-3p40[+]
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Third instar larval eye-antennal imaginal disc clones co-expressing dlg1GD4689 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.CoinFLP-FRT, in combination with Dicer-2 for efficient RNAi, form invasive tumors and induce autophagosomes (assessed by a Atg8a fluorescence reporter) in a cell non-autonomous manner in neighboring disc tissue, which are suppressed and not suppressed, respectively, by the additional clonal co-expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC.
Overexpression of CdsAEY08412 partially, but significantly, rescues the small cell size phenotype found in flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC (when both are expressed in the salivary gland and fat body, under the control of Scer\GAL4ppl.PP). Moreover, the salivary gland ectopic lipid storage phenotype found in Pi3K92ED954A.Scer\UAS.T:Hsap\MYC-expressing flies can be fully suppressed by CdsAEY08412 overexpression.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4arm.PU fails to rescue the reduced fecundity of Ptp61FΔ females.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation. No difference is seen in white prepupae.
Subperineurial expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Gli-rL82 strongly suppresses the egh62d18 nerve phenotype.
In larvae co-expressing CaMKIIT287D.Scer\UAS and Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4D42, the CaMKIIT287D.Scer\UAS-dependent increase in bouton number is completely suppressed and is decreased to a value indistinguishable from that conferred by expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC alone.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4amn-c651 suppresses the CycEJP small eye phenotype.
Heterozygosity for ebi2g2, ebiE90, ebiCC3, ebi2g1, ebiCCS-8, ebiE4, ebiΔ1, ebiW418X, ebik16213, ebiWKS-24 or ebiP7 enhances the small wing phenotype induced by Scer\GAL4Bx-MS1096-mediated expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC.
Co-expression of hppyScer\UAS.cMa and Pi3K92ED954A.Scer\UAS.T:Hsap\MYC using Scer\GAL4nub-AC-62 results in a strong synergistic phenotype of reduced wing size and reduced cell size and number.
A foxoΔ94 mutant background suppresses the extension in lifespan seen upon RU486-induced expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT. Adult onset ubiquitous expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of the RU486-inducible Scer\GAL4da.Switch.PT has no effect on the viability of foxoΔ94 flies. A foxoΔ94 mutant background does not affect the decrease in egg-laying found upon adult-specific ubiquitous expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT. foxoΔ94 mutant flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background). A foxoΔ94 background suppresses the DTT resistance seen in flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT. foxoΔ94 mutant flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).
The small wing phenotype of Scer\GAL4Bx-MS1096>Pi3K92ED954A.Scer\UAS.T:Hsap\MYC flies is enhanced by pix3c2/+ and pix3c3/+.
Flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 and carrying E-2cunspecified have ectopic wing vein tissue.
The ommatidial loss phenotype of foxoGS9928 mutants is strongly enhanced by co-expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF.
Coexpression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF enhances the DJ-1αdsRNA.Scer\UAS-induced eye phenotype.
Co-expression of foxoScer\UAS.cKa and Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF almost completely obliterates the eyes.
The downward curvature of the wing blade caused by expression of banUY3207, banUY1678, banUY789 or banUY1079 under the control of Scer\GAL4Bx-MS1096 is suppressed by co-expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC.
Ectopic expression of Akt1hs.PS suppresses the small eye phenotype resulting from the expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4ey.PH.
The reduction in wing size seen when Pi3K92ED954A.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL4Bx-MS1096 is partially suppressed by Pten1 or Df(2L)flp170B.
Enhances the eye phenotype caused by PtenScer\UAS.cHa, when co-expressed using Scer\GAL4ey.PH.
Xenogenetic Interactions
Statement
Reference
Coexpression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC with Hsap\MAPTV337M.Scer\UAS, under the control of Scer\GAL4GMR.PF has no effect on the Hsap\MAPTV337M.Scer\UAS-induced toxicity in the eye.
Co-expression of Mmus\Foxo1AA.Scer\UAS and Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF almost completely obliterates the eyes.
Complementation and Rescue Data
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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Symbol Synonym
Pi3K92ED954A.Scer\UAS.T:Hsap\MYC
Pi3K92ED954A.UAS.T:Myc
Pi3K92ED954A.UAS.Tag:MYC
Pi3K92E[D954A]
UAS-Dp110DN
Name Synonyms
Secondary FlyBase IDs
    References (69)