Allele Dmel\inaC^{PKCi.Scer\UAS}

General Information
Symbol	Dmel\inaC^{PKCi.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0058456
Feature type	allele	Associated gene	Dmel\inaC

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Broughton et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A PKC inhibitor sequence (the pseudosubstrate domain of inaC) is cloned into the pUAST vector. (Broughton et al., 1996)
Carried in construct	P{UAS-PKCi.B} (Cohen et al., 2002, Aravamudan and Broadie, 2003, Greenspan, 1998.6.18, Broughton et al., 1996, Kane et al., 1997, Meunier et al., 2009, Chen et al., 2008, Lee et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective, with Scer\GAL4^hs.PB (Kane et al., 1997) chemical resistant, with Scer\GAL4^npf.1 (Chen et al., 2008) neurophysiology defective, with Scer\GAL4^MJ94 (Meunier et al., 2009)
Phenotype Manifest In
	egg chamber, with Scer\GAL4^ptc-559.1 (Cohen et al., 2002) germarium, with Scer\GAL4^ptc-559.1 (Cohen et al., 2002) gustatory receptor neuron, with Scer\GAL4^MJ94 (Meunier et al., 2009) ovarian sheath, with Scer\GAL4^ptc-559.1 (Cohen et al., 2002) ovary, with Scer\GAL4^ptc-559.1 (Cohen et al., 2002)
Detailed Description
	Statement Reference Animals expressing inaC[PKCi.Scer\UAS] under the control of Scer\GAL4[MJ94] show a decreased response of the water cells (a specific class of taste receptor neurons) to hypo-osmotic stimuli but not to salts when compared to controls. (Meunier et al., 2009) Flies expressing inaC[PKCi.Scer\UAS] under the control of Scer\GAL4[npf.1] are significantly more resistant to ethanol intoxication relative to paired controls. These flies develop ethanol tolerance to an even higher level than control flies. For example, the time required to anesthetize 50% of flies from ethanol-treated groups is consistently increased by about 1.4-fold relative to controls. The ethanol levels in these flies immediately after exposure to ethanol vapor is the same as in controls, indicating that the flies have similar absorption rates. Further, the turnover rates of ethanol in these flies is the same as controls. (Chen et al., 2008) When expression is governed by Scer\GAL4^ptc-559.1 in apical cells in the ovary the cells show a migration defect. Though some migration does occur the cells do not migrate normally and show a rounded morphology. (Cohen et al., 2002) Heat shocked males are tested for courtship conditioning. They are placed in the same chamber with an unreceptive mated female, males fail to exhibit the effects of conditioning, courtship to the mated females is not suppressed. Despite the failure to show immediate suppression of courtship they do develop normal memory of it. When placed with a virgin female they exhibit reduced courtship. Retention of conditioning is seen over 10 minutes or 2 hours. This result is comparable to non-heat shocked siblings. Males appear to be forming normal memory retention despite their failure to show any sign of associative learning while it is occurring. (Kane et al., 1997) Cell cultures expressing a low concentration of inaC^{PKCi.Scer\UAS} show a significant decrease in the number of type I and II processes as compared to control cultures, while the proportion of neuroblasts, ganglion mother cells and mature neurons in the clusters are little affected. (Broughton et al., 1996)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Flies expressing inaC[PKCi.Scer\UAS] under the control of Scer\GAL4[Oamb.4.4] in a Oamb[unspecified] heterozygous background show wild type ovulation levels. Wild type ovulation levels are also seen when Scer\GAL80[ts.αTub84B] is used to limit expression to 3 days prior to mating. (Lee et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	4589 w^*; P{UAS-PKCi.B}4A
Kyoto	107811 w^*; P{UAS-PKCi.B}4A
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	inaC^{PKCi.Scer\UAS} PKCi^UAS.cBa
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Lee et al., 2009, PLoS ONE 4(3): e4716 The octopamine receptor OAMB mediates ovulation via Ca2+/calmodulin-dependent protein kinase II in the Drosophila oviduct epithelium. [FBrf0207515] Meunier et al., 2009, Chem. Senses 34(5): 441--449 Water taste transduction pathway is calcium dependent in Drosophila. [FBrf0207987] Chen et al., 2008, Neuroscience 156(1): 42--47 A protein kinase C activity localized to neuropeptide Y-like neurons mediates ethanol intoxication in Drosophila melanogaster. [FBrf0206821] Aravamudan and Broadie, 2003, J. Neurobiol. 54(3): 417--438 Synaptic Drosophila UNC-13 is regulated by antagonistic G-protein pathways via a proteasome-dependent degradation mechanism. [FBrf0155886] Cohen et al., 2002, Dev. Cell 2(4): 437--448 DWnt4 regulates cell movement and focal adhesion kinase during Drosophila ovarian morphogenesis. [FBrf0146974] Kane et al., 1997, Neuron 18(2): 307--314 Learning without performance in PKC-deficient Drosophila. [FBrf0092580] Broughton et al., 1996, J. Cell. Biochem. 60(4): 584--599 Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons. [FBrf0086934]
Personal communication to FlyBase	Greenspan, 1998.6.18, UAS-tra and UAS-PKCi UAS-tra and UAS-PKCi [FBrf0102853]

Allele Dmel\inaCPKCi.Scer\UAS

Allele Dmel\inaC^{PKCi.Scer\UAS}