Allele Mmus\Pkaca^mC.Scer\UAS

General Information
Symbol	Mmus\Pkaca^mC.Scer\UAS	Species	M. musculus
Name		FlyBase ID	FBal0058457
Feature type	allele	Associated gene	Mmus\Pkaca

Allele class
Mutagen	FLPase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms sleep defective
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	FLPase (Li et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Mmus\Pkaca^{mC.Scer\FRT.Scer\UAS} (Li et al., 1995)
Nature of the lesion	Statement Reference A constitutively active form of Mmus\Pkaca is expressed under the control of Scer\UAS regulatory sequences. (Ohlmeyer and Kalderon, 1997) Scer\FRT-y^+t7.7 cassette has been excised, juxtaposing Scer\GAL4 Scer\UAS binding sites with mutated Mmus\Pkaca coding sequences (mC*). (Li et al., 1995)
Carried in construct	P{UAS-PKA.mC} (Baines, 2004, Mee et al., 2004, Wang et al., 1999, Yoshihara et al., 2005, Price and Kalderon, 2002, Price and Kalderon, 1999, Dai et al., 2003, Liu et al., 2007, Jia et al., 2005, Jia et al., 2002, Ou et al., 2002, Li et al., 1995, Jackson and Berg, 2002, Kimura et al., 2004, Zhang et al., 2005, Shulman and Feany, 2003, Chyb et al., 1999, Ohlmeyer and Kalderon, 1997, Jia et al., 2004, Venkatesh et al., 2001, Baines, 2003, Davis et al., 1998, Pitman et al., 2006, Joiner et al., 2006, Zhou et al., 2006, Smelkinson and Kalderon, 2006, Kang et al., 2011, Yuan et al., 2011, Jia et al., 2009, Yang et al., 2009, Lee et al., 2009, Cheng et al., 2012, Liu et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective, with Scer\GAL4^30Y (Joiner et al., 2006) behavior defective, with Scer\GAL4^238Y (Joiner et al., 2006) behavior defective, with Scer\GAL4^c309 (Joiner et al., 2006) behavior defective, with Scer\GAL4^Tab2-201Y (Joiner et al., 2006) behavior defective \| heat sensitive, with Scer\GAL4^c309 (Pitman et al., 2006) behavior defective \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Joiner et al., 2006) behavior defective \| RU486 conditional, with Scer\GAL4^{Mef2.247.Switch} (Joiner et al., 2006) developmental rate defective, with Scer\GAL4^{Itp-r83A-1664GAL4} (Venkatesh et al., 2001) increased cell death, with Scer\GAL4^en-e16E (Kimura et al., 2004) lethal, with Scer\GAL4^ap-md544 (Cheng et al., 2012) mating behavior defective \| female, with Scer\GAL4^ppk.PG (Yang et al., 2009) neurophysiology defective, with Scer\GAL4^eve.RN2 (Baines, 2004) neurophysiology defective, with Scer\GAL4^eve.RN2O (Baines, 2003) neurophysiology defective, with Scer\GAL4^Mhc.PW (Davis et al., 1998) sleep defective, with Scer\GAL4^104Y (Liu et al., 2012) temperature response defective \| adult stage, with Scer\GAL4^Mef2.247 (Kang et al., 2011) visible, with Scer\GAL4^en-e16E (Kimura et al., 2004) visual behavior defective, with Scer\GAL4^ninaE.PD (Chyb et al., 1999)
Phenotype Manifest In
	aCC neuron, with Scer\GAL4^eve.RN2O (Baines, 2003) denticle belt \| posterior, with Scer\GAL4^e22c (Ohlmeyer and Kalderon, 1997) nurse cell, with Scer\GAL4^nos.PG (Jackson and Berg, 2002) nurse cell, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Jackson and Berg, 2002) photoreceptor cell, with Scer\GAL4^ninaE.PD (Chyb et al., 1999) RP2 motor neuron, with Scer\GAL4^eve.RN2O (Baines, 2003) wing, with Scer\GAL4^en-e16E (Kimura et al., 2004) wing \| pharate adult stage, with Scer\GAL4^en-e16E (Kimura et al., 2004) wing disc, with Scer\GAL4^71B (Price and Kalderon, 1999)
Detailed Description
	Statement Reference Expression of Mmus\Pkaca[mC.Scer\UAS] under the control of Scer\GAL4[ap-md544] is lethal. (Cheng et al., 2012) Flies expressing Mmus\Pkaca[mC.Scer\UAS] under the control of Scer\GAL4[104Y] show reduced night-time sleep compared to controls. (Liu et al., 2012) Flies expressing Mmus\Pkaca[mC.Scer\UAS] under the control of Scer\GAL4[Mef2.247] show altered temperature preference behaviour compared to wild type: the avoidance index for high temperature is significantly reduced compared to wild type. (Kang et al., 2011) Mated females expressing Mmus\Pkaca[mC.Scer\UAS] under the control of Scer\GAL4[ppk.PG] show higher levels of sexual receptivity than control mated females. The rate of egg-laying of the mutant mated females is not significantly different from that of control mated females. (Yang et al., 2009) Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} (induced using RU486) decreases daily sleep. Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^Tab2-201Y results in an increase in total daily sleep compared to controls. The adults flies have normal waking activity. Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^c309 results in an decrease in total daily sleep compared to controls. Activity normalised to waking time is not significantly higher than controls. Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^30Y or Scer\GAL4^238Y promotes sleep during the day, but inhibits sleep during the night. Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^{Mef2.247.Switch} (in the presence of RU486) results in a reduction in sleep, due to a shortened sleep bout duration without a concomitant increase in the sleep bout number. When RU486 is withdrawn after about three days of sleep deprivation, these flies show a robust sleep rebound response. (Joiner et al., 2006) Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^c309 results in reduced sleep compared to controls. (Pitman et al., 2006) Postsynaptic expression of Mmus\Pkaca^mC.Scer\UAS, under the control of Scer\GAL4^eve.RN2, results in the averaged amplitude of synaptic currents recorded from whole-cell voltage clamp recordings of aCC/RP2 motoneurons being significantly increased compared with controls. (Baines, 2004) Mmus\Pkaca^mC.Scer\UAS; Scer\GAL4^en-e16E adults have blistered wings. There is precocious cell of wing blade cells in late stage pharate adults (G+; <3hrs before eclosion) of this genotype . (Kimura et al., 2004) The ability of aCC/RP2 to fire action potentials (APs) in response to an injection of constant current is decreased when Mmus\Pkaca^mC.Scer\UAS is constitutively expressed under the control of Scer\GAL4^eve.RN2O and there is also a decrease in peak current density of voltage-dependent inward Na⁺ current compared to wild type. (Baines, 2003) 7.3% of egg chambers have at least one binucleate nurse cell in females expressing Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. 3.1% of egg chambers have at least one binucleate nurse cell in females expressing Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^nos.PG. (Jackson and Berg, 2002) When expression is driven by Scer\GAL4^{Itp-r83A-1664GAL4} in an otherwise wild type background larval molting is delayed. Molting delay is most evident at the L2 to L3 transition. Feeding Mmus\Pkaca^mC.Scer\UAS,Scer\GAL4^{Itp-r83A-1664GAL4} larvae 20-hydroxyecdysone has no effect on the molting delay. (Venkatesh et al., 2001) When expression is driven by Scer\GAL4^ninaE.PD photoreceptors have slower macroscopic kinetics, similar to the effect caused by cAMP and IBMX. (Chyb et al., 1999) Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^71B results in a narrowing of the wing disc in the wing blade region. (Price and Kalderon, 1999) Overexpression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^Mhc.PW significantly decreases quantal size as estimated by the average spontaneous miniature excitatory junctional current or miniature excitatory junctional potential recorded in muscle 6 in the absence of nerve stimulation in larvae. The number of boutons at the neuromuscular junction of muscles 6 and 7 is normal in larvae expressing Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^Mhc.PW. (Davis et al., 1998) Embryos expressing Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^e22c give rise to larvae with abnormal denticle belts; the wide posterior denticle rows of each segment are replaced by narrower and often incomplete rows of denticles with different morphology and polarity from wild-type. (Ohlmeyer and Kalderon, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Mmus\Pkaca^mC.Scer\UAS, Scer\GAL4^{Itp-r83A-1664GAL4} has developmental rate defective phenotype, suppressible by Itp-r83A^Scer\UAS.cVa, Scer\GAL4^{Itp-r83A-1664GAL4} (Venkatesh et al., 2001)
Enhancer of
Statement Reference Mmus\Pkaca^mC.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
Suppressor of
Statement Reference Mmus\Pkaca^mC.Scer\UAS/Scer\GAL4^D42 is a suppressor of neuroanatomy defective phenotype of Pka-C1^B3 (Yoshihara et al., 2005) Mmus\Pkaca^mC.Scer\UAS/Scer\GAL4^D42 is a suppressor of neuroanatomy defective phenotype of Syt4^BA1 (Yoshihara et al., 2005)
NOT Suppressor of
Statement Reference Mmus\Pkaca^mC.Scer\UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^Oamb.4.4 is a non-suppressor of female semi-sterile \| recessive phenotype of Oamb^unspecified (Lee et al., 2009) Mmus\Pkaca^mC.Scer\UAS/Scer\GAL4^{Itp-r83A-1664GAL4} is a non-suppressor of developmental rate defective phenotype of Itp-r83A^1664GAL4 (Venkatesh et al., 2001)
Phenotype Manifest In
Enhanced by
Statement Reference Mmus\Pkaca^mC.Scer\UAS, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has nurse cell phenotype, enhanceable by Akap200^k07118a/Df(2L)N22-14 (Jackson and Berg, 2002)
Enhancer of
Statement Reference Mmus\Pkaca^mC.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003) Mmus\Pkaca^mC.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003)
Suppressor of
Statement Reference Mmus\Pkaca^mC.Scer\UAS/Scer\GAL4^D42 is a suppressor of motor neuron phenotype of Pka-C1^B3 (Yoshihara et al., 2005) Mmus\Pkaca^mC.Scer\UAS/Scer\GAL4^D42 is a suppressor of motor neuron phenotype of Syt4^BA1 (Yoshihara et al., 2005)
NOT Suppressor of
Statement Reference Mmus\Pkaca^mC.Scer\UAS, Scer\GAL4^71B is a non-suppressor of wing disc phenotype of Scer\GAL4^71B, ci^{5m.Scer\UAS.T:Ivir\HA1} (Price and Kalderon, 1999) Mmus\Pkaca^mC.Scer\UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^Oamb.4.4 is a non-suppressor of oviduct phenotype of Oamb^unspecified (Lee et al., 2009) Mmus\Pkaca^mC.Scer\UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^Oamb.4.4 is a non-suppressor of uterus phenotype of Oamb^unspecified (Lee et al., 2009)
Other
Statement Reference Akap200^k07118a/Df(2L)N22-14, Mmus\Pkaca^mC.Scer\UAS, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has nurse cell phenotype (Jackson and Berg, 2002)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Mmus\Pkaca[mC.Scer\UAS] under the control of Scer\GAL4[Oamb.4.4], using Scer\GAL80[ts.αTub84B] to limit expression to 3 days prior to mating, fails to suppress the ovulation phenotype seen in Oamb[unspecified] homozygotes. (Lee et al., 2009) The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Mmus\Pkaca^mC.Scer\UAS; the size of the eye is reduced and necrotic foci are formed. (Shulman and Feany, 2003) 30% of egg chambers have at least one binucleate nurse cell in Akap200^k07118a/Df(2L)N22-14 females expressing Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. (Jackson and Berg, 2002) The wing disc phenotype caused by expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^71B is still seen in animals also co-expressing either ci^{WT.P.Scer\UAS.T:Ivir\HA1} or ci^{3m.Scer\UAS.T:Ivir\HA1}. In contrast, in wing discs co-expressing ci^{5m.Scer\UAS.T:Ivir\HA1} and Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^71B, overgrowth of the wing blade region is seen, as in animals expressing ci^{5m.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^71B. (Price and Kalderon, 1999) When expressed with Pka-R1^BDK.Scer\UAS or Pka-R1^BGD.Scer\UAS under the influence of Scer\GAL4^E62-2, Scer\GAL4^h-1J3 or Scer\GAL4^C166-2, Mmus\Pkaca^mC.Scer\UAS completely suppresses adult pattern defects elicited by Pka-R1^BDK.Scer\UAS or Pka-R1^BGD.Scer\UAS. Adults have normal legs, blistered wings, no cuticular outgrowths or duplicated wings. (Li et al., 1995)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Exists in clones, not reported as being germ-line transmitted. (Li et al., 1995)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Mmus\Pkaca^mC.Scer\UAS PKA^mC.UAS
Name Synonym
Secondary FlyBase IDs

References ( 34 )

Generate a list of	All references relating to this allele ( 34 )

List References by type	Research paper ( 34 )
Recent research papers ( 4 )
	Cheng et al., 2012, Development 139(1): 85--94 Drosophila G-protein-coupled receptor kinase 2 regulates cAMP-dependent Hedgehog signaling. [FBrf0216860] Liu et al., 2012, Curr. Biol. 22(22): 2114--2123 Two dopaminergic neurons signal to the dorsal fan-shaped body to promote wakefulness in Drosophila. [FBrf0220041] Kang et al., 2011, PLoS ONE 6(12): e29800 Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila. [FBrf0217065] Yuan et al., 2011, Science 333(6048): 1458--1462 Light-induced structural and functional plasticity in Drosophila larval visual system. [FBrf0215276] Show all research papers ...

Allele Mmus\PkacamC.Scer\UAS

Allele Mmus\Pkaca^mC.Scer\UAS