FlyBase Allele Report: Dmel\N[ECN.UAS]

General Information

Symbol

Dmel\N^ECN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0058505

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.ECN}

Also Known As

UAS-N^ECN, UAS-N^ECD, UAS-N^DN, UAS-Notch^ECN, UAS-ECN, UAS-Necd, UASECN, N^dn, UAS-N^EC

Key Links

Transgenic product class

dominant_negative_variant

(Jacobsen et al., 1998)

inframe_deletion

(Jacobsen et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Jacobsen et al., 1998)

inframe_deletion

(Jacobsen et al., 1998)

Mutagen

in vitro construct

(Brennan et al., 1999, de Celis et al., 1998, Jacobsen et al., 1998, Klein and Martinez Arias, 1998, Jonsson and Knust, 1996)

Progenitor genotype

Carried in construct

P{UAS-N.ECN}

Cytology

Description

UASt regulatory sequences drive amino acids 1 to 1789 of N. This results in a protein which lacks most of the intracellular domain and which acts as a dominant-negative.

(Jacobsen et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Src64B^UAS.cCa

(Enomoto et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior, with Scer\GAL4^shot-OK307

(Presente et al., 2002)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ato.3.6

(Langen et al., 2013)

abnormal planar polarity, with Scer\GAL4^salm-459.2

(Cooper and Bray, 1999)

abnormal size | third instar larval stage, with Scer\GAL4^en.PU

(Slaninova et al., 2016)

decreased cell number | wandering third instar larval stage, with Scer\GAL4^btl.PS

(Pitsouli and Perrimon, 2013)

increased cell death, with Scer\GAL4^LL54

(Peralta et al., 2009)

visible, with Scer\GAL4^bbg-C96

(Presente et al., 2002)

visible, with Scer\GAL4^bi-omb-Gal4

(Pueyo and Couso, 2011)

visible, with Scer\GAL4^dpp.3KK

(Molnar et al., 2006)

visible, with Scer\GAL4^dpp.blk1

(de Celis et al., 1998)

visible, with Scer\GAL4^ey.PH

(Chern and Choi, 2002)

visible, with Scer\GAL4^GMR.PF

(Chern and Choi, 2002, Presente et al., 2002)

visible, with Scer\GAL4^hs.2sev

(Presente et al., 2002)

visible, with Scer\GAL4^hs.PB

(Barolo et al., 2000)

visible, with Scer\GAL4^klu-G410

(Bishop et al., 1999)

visible, with Scer\GAL4^pnr-MD237

(Presente et al., 2002)

visible, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000, Brennan et al., 1999)

visible, with Scer\GAL4^sca-109-68

(Okabe et al., 2001)

visible, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

visible, with Scer\GAL4^Ubx.PdC

(de Celis and Bray, 1997)

Phenotype Manifest In

adult thorax & microchaeta, with Scer\GAL4^pnr-MD237

(Presente et al., 2002)

dMP2 neuron, with Scer\GAL4^pros.PMG

(O'Connor-Giles and Skeath, 2003)

embryonic/larval anterior spiracle, with Scer\GAL4^klu-G410

(Klein and Martinez Arias, 1998)

embryonic/larval dorsal branch & tracheal tip cell, with Scer\GAL4^btl.PS

(Ikeya and Hayashi, 1999)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^btl.PS

(Ikeya and Hayashi, 1999)

embryonic heart cardioblast | embryonic stage 16, with Scer\GAL4^twi.PG

(Babaoglan et al., 2009)

embryonic neuroblast, with Scer\GAL4^{VP16.F442A.αTub84B}

(Struhl and Greenwald, 2001)

embryonic pericardial cell | embryonic stage 12, with Scer\GAL4^twi.PG

(Babaoglan et al., 2009)

embryonic pericardial cell | embryonic stage 15, with Scer\GAL4^twi.PG

(Babaoglan et al., 2009)

embryonic pericardial cell | embryonic stage 16, with Scer\GAL4^twi.PG

(Babaoglan et al., 2009)

embryonic proventriculus, with Scer\GAL4^hs.PB

(Fuss et al., 2004)

external sensory organ, with Scer\GAL4^sca-109-68

(Okabe et al., 2001)

eye, with Scer\GAL4^ey.PH

(Chern and Choi, 2002, Dominguez and de Celis, 1998)

eye, with Scer\GAL4^GMR.PF

(Chern and Choi, 2002, Presente et al., 2002)

eye disc, with Scer\GAL4^ey.PH

(Dominguez et al., 2004)

keyhole structure, with Scer\GAL4^hs.PB

(Fuss et al., 2004)

larval EL neuron, with Scer\GAL4^pros.PMG

(O'Connor-Giles and Skeath, 2003)

larval RP2 motor neuron, with Scer\GAL4^pros.PMG

(O'Connor-Giles and Skeath, 2003)

leg, with Scer\GAL4^bi-omb-Gal4

(Pueyo and Couso, 2011)

leg, with Scer\GAL4^dpp.blk1

(de Celis et al., 1998)

leg, with Scer\GAL4^klu-G410

(Bishop et al., 1999)

leg & joint, with Scer\GAL4^klu-G410

(Bishop et al., 1999)

leg joint, with Scer\GAL4^bi-omb-Gal4

(Pueyo and Couso, 2011)

lobula columnar neuron LC14, with Scer\GAL4^ato.3.6

(Langen et al., 2013)

macrochaeta, with Scer\GAL4^ptc-559.1

(Brennan et al., 1999)

macrochaeta & mesothoracic tergum, with Scer\GAL4^sca-109-68

(Jafar-Nejad et al., 2005)

macrochaeta | increased number, with Scer\GAL4^pnr-MD237

(Presente et al., 2002)

medulla, with Scer\GAL4^ato.3.6

(Langen et al., 2013)

ommatidium, with Scer\GAL4^GMR.PF

(Chern and Choi, 2002, Presente et al., 2002)

ommatidium, with Scer\GAL4^hs.2sev

(Presente et al., 2002)

ommatidium, with Scer\GAL4^salm-459.2

(Cooper and Bray, 1999)

photoreceptor cell R4, with Scer\GAL4^salm-459.2

(Cooper and Bray, 1999)

proventriculus inner layer primordium, with Scer\GAL4^hs.PB

(Fuss et al., 2004)

proventriculus intermediate layer primordium, with Scer\GAL4^hs.PB

(Fuss et al., 2004)

RP2sib neuron | increased number, with Scer\GAL4^pros.PMG

(O'Connor-Giles and Skeath, 2003)

secondary pigment cell, with Scer\GAL4^LL54

(Peralta et al., 2009)

sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

tarsal segment, with Scer\GAL4^dpp.blk1

(de Celis et al., 1998)

tertiary pigment cell, with Scer\GAL4^LL54

(Peralta et al., 2009)

tormogen cell, with Scer\GAL4^hs.PB

(Barolo et al., 2000)

tormogen cell, with Scer\GAL4^sca-109-68

(Okabe et al., 2001)

tormogen cell & mesothoracic tergum | supernumerary, with Scer\GAL4^sca-109-68

(Jafar-Nejad et al., 2005)

tormogen cell | increased number, with Scer\GAL4^sca-109-68

(Rajan et al., 2009)

tracheolar cell | wandering third instar larval stage, with Scer\GAL4^btl.PS

(Pitsouli and Perrimon, 2013)

trichogen cell, with Scer\GAL4^sca-109-68

(Okabe et al., 2001)

trichogen cell | ectopic, with Scer\GAL4^hs.PB

(Barolo et al., 2000)

U neuron | increased number, with Scer\GAL4^pros.PMG

(O'Connor-Giles and Skeath, 2003)

Usib neuron, with Scer\GAL4^pros.PMG

(O'Connor-Giles and Skeath, 2003)

vMP2 neuron | increased number, with Scer\GAL4^pros.PMG

(O'Connor-Giles and Skeath, 2003)

wing, with Scer\GAL4^bbg-C96

(Presente et al., 2002)

wing, with Scer\GAL4^Bx-MS1096

(Klein et al., 1997)

wing, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999, Klein et al., 1997)

wing, with Scer\GAL4^Ubx.PdC

(de Celis and Bray, 1997)

wing disc, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000, Jonsson and Knust, 1996)

wing disc, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Slaninova et al., 2016)

wing margin, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

wing margin bristle, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing vein, with Scer\GAL4^dpp.3KK

(Molnar et al., 2006)

wing vein, with Scer\GAL4^Ubx.PdC

(de Celis and Bray, 1997)

Detailed Description

Statement

Reference

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^en.PU results in a significant decrease in size of the third instar wing disc posterior compartment, as compared to controls.

(Slaninova et al., 2016)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ato.3.6 results in clustering of dorsal cluster neuron axons. There is a significant increase in the number of axons targeting the medulla compared to wild type.

63% of single cell dorsal cluster neuron clones expressing N^ECN.Scer\UAS under the control of Scer\GAL4^ato.3.6 innervate the medulla, compared to only 24% of control clones.

(Langen et al., 2013)

In spiracular branch growth zones 1 and 2, expression of N^ECN.UAS under the control of Scer\GAL4^btl.PS (in combination with a Gal80[ts] transgene to ensure careful control of N^ECN.UAS overexpression in time and viability of the larvae to the late L3 stage) leads to a decreased spiracular branch cell number.

(Pitsouli and Perrimon, 2013)

Males expressing N^ECN.Scer\UAS under the control of Scer\GAL4^Abd-B-LDN show normal genitalia rotation.

(Lee et al., 2011)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^bi-omb-Gal4 results in deformed legs with incomplete joints.

(Pueyo and Couso, 2011)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^twi.PG leads to a near complete loss of svp-positive heart precursors and heart cells.

eve-positive equivalence groups form when N^ECN.Scer\UAS is driven under the control of Scer\GAL4^twi.PG, however few precursors and eve-positive pericardial cells are seen in this background.

(Babaoglan et al., 2009)

Expression of N^ECN.Scer\UAS in the eye under the control of Scer\GAL4^LL54 results in an increase in interommatidial pigment cell death.

(Peralta et al., 2009)

Scer\GAL4^sca-109-68-mediated expression of N^ECN.Scer\UAS results in a multiple socket phenotype.

(Rajan et al., 2009)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^dpp.3KK results in a strong wing vein thickening phenotype.

(Molnar et al., 2006)

In N^ECN.Scer\UAS; Scer\GAL4^sca-109-68, bristles are transformed so that they have two sockets and no shaft, a phenotype indicative of PIIa to PIIb transformation.

(Jafar-Nejad et al., 2005)

Overexpression of one copy of N^ECN.Scer\UAS under the control of Scer\GAL4^ey.PH leads to a reduction of eye-disc size. Overexpression of two copies of N^ECN.Scer\UAS, also under control of Scer\GAL4^ey.PH, leads to a more extensive eye-disc loss.

(Dominguez et al., 2004)

In N^ECN.Scer\UAS; Scer\GAL4^hs.PB embryos, the ectodermal keyhole cells fail to complete their inward movement.

(Fuss et al., 2004)

N^ECN.Scer\UAS; Scer\GAL4^pros.PMG 10% of RP2 neurons are transformed into RP2 sib neurons, 94% of dMP2 neurons are converted to vMP2 and 97% of hemi-segments exhibit an increase in U neurons.

(O'Connor-Giles and Skeath, 2003)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ey.PH results in adults with small eyes or no eyes. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^GMR.PF results in rough eyes which are relatively normal in size.

(Chern and Choi, 2002)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^C96 results in ragged wings and accompanying bristle loss. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^hs.2sev results in a very mild rough eye phenotype with very low penetrance. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype and excess bristle formation. Animals expressing N^ECN.Scer\UAS under the control of Scer\GAL4^pnr-MD237 show loss of microchaetae on the thorax and multiplication of macrochaetae. Animals expressing N^ECN.Scer\UAS under the control of Scer\GAL4^A307 have a markedly impaired startle response to proprionic acid vapour compared to controls.

(Presente et al., 2002)

When expression is driven by Scer\GAL4^sca-109-68 flies show a balding phenotype due to loss of socket and shaft cells.

(Okabe et al., 2001)

N^ECN.Scer\UAS when driven by Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} suppresses neuroblast segregation in the embryo.

(Struhl and Greenwald, 2001)

Pupae in which N^ECN.Scer\UAS is expressed under the control of Scer\GAL4^hs.PB using heat shock at 37^oC for 2 hours during the tormogen-trichogen cell fate decision show socket-to-shaft cell fate transformations.

(Barolo et al., 2000)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ato.3.6 has no effect on the formation of the larval or adult axonal patterns.

(Hassan et al., 2000)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ptc-559.1 results in an ectopic fold in the wing pouch along the anterior/posterior boundary. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ptc-559.1 results in a nick at the wing margin between veins 3 and 4.

(Lawrence et al., 2000)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^klu-G410 produces shortened legs due to the loss of interjoint tissue. The leg joints are still present in these flies but are sometimes fused.

(Bishop et al., 1999)

Expression of N^ECN.Scer\UAS at 17^oC during larval development throughout the wing by Scer\GAL4^sd-SG29.1 disrupts its development. Cell death is concentrated around the margin region of the wing disc. The wing pouch is smaller than wild-type. Wings in these adult flies are almost completely missing wing margin. Expression of N^ECN.Scer\UAS at 17^oC driven by Scer\GAL4^sd-SG29.1 causes a reduction of the number of SOPs (sensory organ precursors) seen in the wing disc.

(Brennan et al., 1999)

Expression of N^ECN.Scer\UAS driven by Scer\GAL4^ptc-559.1 at 25^oC results in a loss of scutellar bristles.

(Brennan et al., 1999)

Flies expressing N^ECN.Scer\UAS under the control of Scer\GAL4^salm-459.2 develop symmetrical ommatidia with the shape expected for ommatidia containing two R3 photoreceptor cells (more than 61% of ommatidia have this phenotype).

(Cooper and Bray, 1999)

When N^ECN.Scer\UAS is expressed in embryos under the control of Scer\GAL4^btl.PS, the number of esg-expressing cells at the tip of the dorsal branch in the tracheal system is increased up to four.

(Ikeya and Hayashi, 1999)

When expression is driven by Scer\GAL4^ey.PH, the eye is very much reduced, or missing altogether.

(Dominguez and de Celis, 1998)

Flies expressing N^ECN.Scer\UAS under the control of Scer\GAL4^{l(3)31-1-31-1} do not show any bristle defects.

(Jacobsen et al., 1998)

Larvae expressing N^ECN.Scer\UAS under the control of Scer\GAL4^klu-G410 lack the anterior spiracle.

(Klein and Martinez Arias, 1998)

Flies expressing N^ECN.Scer\UAS under the control of Scer\GAL4^dpp.blk1 show disruption of the joints between tarsal segments, resulting in shortened legs.

(de Celis et al., 1998)

Scer\GAL4^sd-SG29.1- or Scer\GAL4^Bx-MS1096-mediated expression causes loss of the majority of wing tissue.

(Klein et al., 1997)

Clones in the wing are frequently associated with nicks in the wing margin and cause the differentiation of thicker veins.

(de Celis and Bray, 1997)

Scer\GAL4^ptc-559.1-mediated expression causes a nick in the distal wing margin and a thickening of the third wing vein.

(Jonsson and Knust, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^ECN.UAS, Scer\GAL4^ato.3.6 has abnormal neuroanatomy | adult stage phenotype, suppressible by bsk^DN.UAS, Scer\GAL4^ato.3.6

(Langen et al., 2013)

N^ECN.UAS, Scer\GAL4^ato.3.6 has abnormal neuroanatomy | adult stage phenotype, suppressible by Pak^KK101874, Scer\GAL4^ato.3.6

(Langen et al., 2013)

N^ECN.UAS, Scer\GAL4^btl.PS has decreased cell number | wandering third instar larval stage phenotype, suppressible by ct^GD1237, Scer\GAL4^btl.PS

(Pitsouli and Perrimon, 2013)

N^ECN.UAS, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by PRAS40^UAS.cCa, Scer\GAL4^ey.PH

(Chern and Choi, 2002)

N^ECN.UAS, Scer\GAL4^sd-SG29.1 has visible phenotype, suppressible by wg^l-12.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

N^ECN.UAS, Scer\GAL4^sd-SG29.1 has visible phenotype, suppressible by wg^UAS.cGa, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

NOT suppressed by

Statement

Reference

N^ECN.UAS, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by PRAS40^UAS.cCa, Scer\GAL4^GMR.PF

(Chern and Choi, 2002)

Suppressor of

Statement

Reference

N^ECN.UAS/Scer\GAL4^LL54 is a suppressor of increased cell death phenotype of Chc⁴

(Peralta et al., 2009)

N^ECN.UAS, Scer\GAL4^ptc-559.1 is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

Scer\GAL4^ptc-559.1/N^ECN.UAS is a suppressor of visible phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of visible phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

NOT Suppressor of

Statement

Reference

N^ECN.UAS, Scer\GAL4^ato.3.6 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Pak^KK101874, Scer\GAL4^ato.3.6

(Langen et al., 2013)

N^ECN.UAS, Scer\GAL4^ato.3.6 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^ato.3.6, bsk^DN.UAS

(Langen et al., 2013)

N^ECN.UAS/Scer\GAL4^sca-109-68 is a non-suppressor of visible | recessive phenotype of msi¹

(Okabe et al., 2001)

Other

Statement

Reference

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has visible phenotype

(Escudero et al., 2003)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has visible phenotype

(Okabe et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

N^ECN.UAS, Scer\GAL4^pros.PMG has larval EL neuron phenotype, non-enhanceable by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has larval RP2 motor neuron phenotype, non-enhanceable by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has RP2sib neuron | increased number phenotype, non-enhanceable by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has U neuron | increased number phenotype, non-enhanceable by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has Usib neuron phenotype, non-enhanceable by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has dMP2 neuron phenotype, non-enhanceable by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has vMP2 neuron | increased number phenotype, non-enhanceable by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

Suppressed by

Statement

Reference

N^ECN.UAS, Scer\GAL4^ato.3.6 has medulla phenotype, suppressible by bsk^DN.UAS, Scer\GAL4^ato.3.6

(Langen et al., 2013)

N^ECN.UAS, Scer\GAL4^ato.3.6 has medulla phenotype, suppressible by Pak^KK101874, Scer\GAL4^ato.3.6

(Langen et al., 2013)

N^ECN.UAS, Scer\GAL4^btl.PS has tracheolar cell | wandering third instar larval stage phenotype, suppressible by ct^GD1237, Scer\GAL4^btl.PS

(Pitsouli and Perrimon, 2013)

N^ECN.UAS, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by toe^UAS.cAa, Scer\GAL4^ey.PH

(Dominguez et al., 2004)

N^ECN.UAS, Scer\GAL4^ey.PH has eye disc phenotype, suppressible by eyg^UAS.lune, Scer\GAL4^ey.PH

(Dominguez et al., 2004)

N^ECN.UAS, Scer\GAL4^ey.PH has eye | ventral phenotype, suppressible | partially by PRAS40^UAS.cCa, Scer\GAL4^ey.PH

(Chern and Choi, 2002)

N^ECN.UAS, Scer\GAL4^sd-SG29.1 has wing vein phenotype, suppressible by wg^l-12.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

N^ECN.UAS, Scer\GAL4^sd-SG29.1 has wing disc phenotype, suppressible by wg^UAS.cGa, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

N^ECN.UAS, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible by wg^UAS.cGa, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

NOT suppressed by

Statement

Reference

N^ECN.UAS, Scer\GAL4^pros.PMG has larval EL neuron phenotype, non-suppressible by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has RP2sib neuron | increased number phenotype, non-suppressible by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has U neuron | increased number phenotype, non-suppressible by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has Usib neuron phenotype, non-suppressible by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has dMP2 neuron phenotype, non-suppressible by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has vMP2 neuron | increased number phenotype, non-suppressible by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^pros.PMG has larval RP2 motor neuron phenotype, non-suppressible by spdo^G104/spdo^G104

(O'Connor-Giles and Skeath, 2003)

N^ECN.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by PRAS40^UAS.cCa, Scer\GAL4^GMR.PF

(Chern and Choi, 2002)

N^ECN.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by PRAS40^UAS.cCa, Scer\GAL4^GMR.PF

(Chern and Choi, 2002)

Suppressor of

Statement

Reference

N^ECN.UAS/Scer\GAL4^LL54 is a suppressor of secondary pigment cell phenotype of Chc⁴

(Peralta et al., 2009)

N^ECN.UAS/Scer\GAL4^LL54 is a suppressor of tertiary pigment cell phenotype of Chc⁴

(Peralta et al., 2009)

N^ECN.UAS/Scer\GAL4^αTub84B.PC is a suppressor of wing disc phenotype of ap^56f

(Zecca and Struhl, 2007)

N^ECN.UAS/Scer\GAL4^sca-109-68 is a suppressor of macrochaeta & mesothoracic tergum | somatic clone phenotype of Sec15³

(Jafar-Nejad et al., 2005)

N^ECN.UAS, Scer\GAL4^Dll-md23 is a suppressor of eye | ectopic phenotype of Scer\GAL4^Dll-md23, danr^UAS.cSa

(Suzanne et al., 2003)

N^ECN.UAS, Scer\GAL4^ptc-559.1 is a suppressor of wing margin | ectopic phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

N^ECN.UAS, Scer\GAL4^ptc-559.1 is a suppressor of wing phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

Scer\GAL4^ptc-559.1/N^ECN.UAS is a suppressor of wing margin | ectopic phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

Scer\GAL4^ptc-559.1/N^ECN.UAS is a suppressor of wing phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of presutural bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior notopleural bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior notopleural bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior supraalar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior postalar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior postalar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior dorsocentral bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior scutellar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^ECN.UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior scutellar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

NOT Suppressor of

Statement

Reference

N^ECN.UAS, Scer\GAL4^ato.3.6 is a non-suppressor of medulla phenotype of Pak^KK101874, Scer\GAL4^ato.3.6

(Langen et al., 2013)

N^ECN.UAS, Scer\GAL4^ato.3.6 is a non-suppressor of medulla phenotype of Scer\GAL4^ato.3.6, bsk^DN.UAS

(Langen et al., 2013)

N^ECN.UAS/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

N^ECN.UAS/Scer\GAL4^sca-109-68 is a non-suppressor of external sensory organ phenotype of msi¹

(Okabe et al., 2001)

N^ECN.UAS/Scer\GAL4^sca-109-68 is a non-suppressor of trichogen cell | increased number phenotype of msi¹

(Okabe et al., 2001)

N^ECN.UAS/Scer\GAL4^{VP16.F442A.αTub84B} is a non-suppressor of embryonic neuroblast | increased number phenotype of Psn^C2

(Struhl and Greenwald, 2001)

Other

Statement

Reference

N^ECN.UAS, Scer\GAL4^sca-109-68 has tormogen cell | increased number phenotype

(Rajan et al., 2009)

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has macrochaeta phenotype

(Escudero et al., 2003)

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has macrochaeta | ectopic phenotype

(Escudero et al., 2003)

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has microchaeta phenotype

(Escudero et al., 2003)

N^ECN.UAS, Nct^R46, Scer\GAL4^unspecified has wing hinge phenotype

(Chung and Struhl, 2001)

N^ECN.UAS, Nct^R46, Scer\GAL4^unspecified has sensory mother cell | ectopic phenotype

(Chung and Struhl, 2001)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has external sensory organ phenotype

(Okabe et al., 2001)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has trichogen cell phenotype

(Okabe et al., 2001)

N^ECN.UAS, Scer\GAL4^sca-109-68, msi¹ has eo neuron phenotype

(Okabe et al., 2001)

N^ECN.UAS, Psn^C2, Scer\GAL4^{VP16.F442A.αTub84B} has wing vein phenotype

(Struhl and Greenwald, 2001)

N^ECN.UAS, Psn^C2, Scer\GAL4^{VP16.F442A.αTub84B} has wing margin phenotype

(Struhl and Greenwald, 2001)

N^ECN.UAS, Psn^C2, Scer\GAL4^{VP16.F442A.αTub84B} has sensory mother cell | increased number phenotype

(Struhl and Greenwald, 2001)

N^ECN.UAS, Psn^αTub84B.PS, Psn^C2, Scer\GAL4^{VP16.F442A.αTub84B} has wing | ectopic phenotype

(Struhl and Greenwald, 2001)

N^ECN.UAS, Scer\GAL4^sd-SG29.1, arm^{S10.UAS.Tag:MYC} has wing disc phenotype

(Brennan et al., 1999)

N^ECN.UAS, Scer\GAL4^sd-SG29.1, arm^{S10.UAS.Tag:MYC} has sensory mother cell & dorsal mesothoracic disc phenotype

(Brennan et al., 1999)

N^ECN.UAS, Scer\GAL4^sd-SG29.1, arm^UAS.cSa has sensory mother cell & dorsal mesothoracic disc phenotype

(Brennan et al., 1999)

N^ECN.UAS, Scer\GAL4^sd-SG29.1, arm^{S10.UAS.Tag:MYC} has dorsal mesothoracic disc & sensory mother cell phenotype

(Brennan et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The number of medulla axons in animals co-expressing both bsk^DN.Scer\UAS and N^ECN.Scer\UAS under the control of Scer\GAL4^ato.3.6 is similar to that seen in animals expressing bsk^DN.Scer\UAS alone under the control of Scer\GAL4^ato.3.6.

The number of medulla axons in animals co-expressing both Pak^KK101874 and N^ECN.Scer\UAS under the control of Scer\GAL4^ato.3.6 is indistinguishable from seen in animals expressing Pak^KK101874 alone under the control of Scer\GAL4^ato.3.6.

(Langen et al., 2013)

In spiracular branch growth zones 1 and 2, expression of ct^GD1237 under the control of Scer\GAL4^btl.PS partially suppresses the decreased spiracular branch cell number phenotype caused by expression of N^ECN.UAS under the control of Scer\GAL4^btl.PS (in combination with a Gal80[ts] transgene to ensure careful control of N^ECN.UAS overexpression in time and viability of the larvae to the late L3 stage).

(Pitsouli and Perrimon, 2013)

Expression of N^ECN.Scer\UAS in the eye under the control of Scer\GAL4^LL54 completely suppresses the interommatidial cell death phenotype observed in Chc⁴ mutant flies.

(Peralta et al., 2009)

Scer\GAL4^sca-109-68-mediated expression of N^ECN.Scer\UAS in Arp3^83F clones, as in a wild type background, results in a multiple socket phenotype.

(Rajan et al., 2009)

Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^αTub84B.PC in ap^56f/ap^56f wing discs rescues wing growth.

(Zecca and Struhl, 2007)

Scer\GAL4^ey.PH-mediated expression of eyg^{Scer\UAS.lune} fully rescues the defective eye phenotype of mutants expressing two copies of N^ECN.Scer\UAS, also under the control of Scer\GAL4^ey.PH. Expression of eyg^{Scer\UAS.lune} fully and consistently rescues the eye-size defect in N^ECN.Scer\UAS mutants, where expression is driven by Scer\GAL4^ey.PH. Expression of toe^Scer\UAS.cAa, under control of Scer\GAL4^ey.PH, partially rescues the eye-size defect of N^ECN.Scer\UAS mutants.

(Dominguez et al., 2004)

Co-expression of N^ECN.Scer\UAS and ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-C253 results in loss of most microchaetae on the notum and either loss of macrochaetae or tufts of bristles in their place. Clusters of neurons appear at the sites of the developing sensory organs in pupal notum.

(Escudero et al., 2003)

The transformation of neurons (RP2 to RP2sib; dMP2 to vMP2; Usib to U) seen in N^ECN.Scer\UAS; Scer\GAL4^pros.PMG embryos is unaffected by spdo^G104/spdo^G104.

(O'Connor-Giles and Skeath, 2003)

The reduced eye phenotype caused by expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ey.PH is partially suppressed in the ventral part of the eye by coexpression of CG46146^Scer\UAS.cCa. The rough eye phenotype caused by expression of N^ECN.Scer\UAS under the control of Scer\GAL4^GMR.PF is not suppressed by coexpression of CG46146^Scer\UAS.cCa.

(Chern and Choi, 2002)

N^ECN.Scer\UAS (Scer\GAL4^sca-109-68), msi¹ double mutants show a dense double bristle phenotype. The IIb precursor takes the non-neuronal fate as there are no neurons in the subepidermal layer.

(Okabe et al., 2001)

In Psn^C2 clones that also express N^ECN.Scer\UAS (under the control of Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16}) formation of multiple sensory organ mother cell (SMCs) is seen in place of single SMCs. Psn^C2 clones that also express N^ECN.Scer\UAS, give wing phenotypes. Clones that cross the wing margin lead to wing notching. Clones within the dorsal compartment of the blade can form abnormally thick veins. If Psn^αTub84B.PS is also added, a double dorsal wing outgrowth develops, flanked by adjacent rows of dorsal wing margin bristles.

In Psn^C2 wing disc clones that also express N^ECN.Scer\UAS (under the control of Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16}) formation of multiple sensory organ mother cell (SMCs) is seen in place of single SMCs.

Psn^C2 clones that also express N^ECN.Scer\UAS, give adult wing phenotypes: clones that cross the wing margin lead to wing notching, whereas clones within the dorsal compartment of the blade can form abnormally thick veins. If Psn^αTub84B.PS is also added, a double dorsal wing outgrowth develops, flanked by adjacent rows of dorsal wing margin bristles.

(Struhl and Greenwald, 2001)

The wing phenotype caused by expression of Ser^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 is suppressed by coexpression of N^ECN.Scer\UAS. The ectopic margin caused by expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 is suppressed by coexpression of N^ECN.Scer\UAS, except for a small patch on the dorsal side, and the wing is almost wild type in shape.

(Lawrence et al., 2000)

The addition of wg^Scer\UAS.cGa suppresses the wing pouch phenotype seen in N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 wing discs and leads to cell death occurring over the whole of the wing pouch. Wings in these adult flies are small and bear a small number of bristles. The addition of wg^{l-12.Scer\UAS} suppresses the wing pouch phenotype seen in N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 wing discs, and abolishes the cell death phenotype. Wings in these adult flies are almost wild-type in size and in many instances display a partial restoration of bristles at the edge. In addition these wings display enlarged veins which are much broader than those produced by either N^ECN.Scer\UAS or wg^{l-12.Scer\UAS} alone. The addition of arm^{S10.Scer\UAS.T:Hsap\MYC} to N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 flies have smaller wing pouches, In the adult wing they show a combination of the phenotypes seen in flies expressing N^ECN.Scer\UAS and arm^{S10.Scer\UAS.T:Hsap\MYC} alone i.e. the adults wings are small, lacking a margin and are covered in bristles. The addition of wg^{l-12.Scer\UAS} has no effect on the sensory organ precursor phenotype of animals expressing N^ECN.Scer\UAS driven by Scer\GAL4^sd-SG29.1at 17^oC. The addition of arm^{S10.Scer\UAS.T:Hsap\MYC} leads to an increase in the area covered by SOPs, though they are not evenly spaced. The addition of wg^Scer\UAS.cGa leads to an increase in the area covered by SOPs, similar to when wg^Scer\UAS.cGa is expressed alone, though they are not evenly spaced.

(Brennan et al., 1999)

Coexpression of N^ECN.Scer\UAS completely suppresses the transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by Dl^Scer\UAS.cJa expressed under the control of Scer\GAL4^{l(3)31-1-31-1}.

(Jacobsen et al., 1998)

Coexpression of N^ECN.Scer\UAS and Ser^Scer\UAS.cSa under the control of Scer\GAL4^sd-SG29.1 demonstrates Ser can partially suppress the effect of N : wings are smaller than wild type, the dorsal surface is smaller than the ventral surface.

(Klein et al., 1997)

Scer\GAL4^ptc-559.1-mediated coexpression with Ser^Scer\UAS.cSa allows most flies to hatch at 25^oC, both phenotypes provoked by Ser^Scer\UAS.cSa alone (extra wing tissue on the ventral side and formation of a notch in the margin) are strongly suppressed.

(Jonsson and Knust, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

N^ECN.UAS is partially rescued by Scer\GAL4^sd-SG29.1/N^int.I.UAS

(Brennan et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51667

P{hsFLP}12, y¹ w^*; P{UAS-N.ECN}2; MKRS/TM2

Notes on Origin

Discoverer

M. Baylies.

(de Celis and Bray, 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

N^DN

(Langen et al., 2013)

N^ECN.Scer\UAS

N^ECN.UAS

N^dn

(Cooper and Bray, 1999)

N^ecd.UAS

Name Synonyms

Secondary FlyBase IDs

FBal0066008

References (60)

Report Sections

Open Close