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General Information
Symbol
Dmel\NECN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0058505
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-NECN, UAS-NECD, UAS-NDN, UAS-NotchECN, UAS-ECN, UAS-Necd, UASECN, Ndn, UAS-NEC
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive amino acids 1 to 1789 of N. This results in a protein which lacks most of the intracellular domain and which acts as a dominant-negative.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

adult thorax & microchaeta, with Scer\GAL4pnr-MD237

embryonic/larval dorsal branch & tracheal tip cell, with Scer\GAL4btl.PS

macrochaeta & mesothoracic tergum, with Scer\GAL4sca-109-68

sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4sd-SG29.1

tormogen cell & mesothoracic tergum | supernumerary, with Scer\GAL4sca-109-68

Detailed Description
Statement
Reference

Expression of NECN.Scer\UAS under the control of Scer\GAL4en.PU results in a significant decrease in size of the third instar wing disc posterior compartment, as compared to controls.

Expression of NECN.Scer\UAS under the control of Scer\GAL4ato.3.6 results in clustering of dorsal cluster neuron axons. There is a significant increase in the number of axons targeting the medulla compared to wild type.

63% of single cell dorsal cluster neuron clones expressing NECN.Scer\UAS under the control of Scer\GAL4ato.3.6 innervate the medulla, compared to only 24% of control clones.

Males expressing NECN.Scer\UAS under the control of Scer\GAL4Abd-B-LDN show normal genitalia rotation.

Expression of NECN.Scer\UAS under the control of Scer\GAL4bi-omb-Gal4 results in deformed legs with incomplete joints.

Expression of NECN.Scer\UAS under the control of Scer\GAL4twi.PG leads to a near complete loss of svp-positive heart precursors and heart cells.

eve-positive equivalence groups form when NECN.Scer\UAS is driven under the control of Scer\GAL4twi.PG, however few precursors and eve-positive pericardial cells are seen in this background.

Expression of NECN.Scer\UAS in the eye under the control of Scer\GAL4LL54 results in an increase in interommatidial pigment cell death.

Scer\GAL4sca-109-68-mediated expression of NECN.Scer\UAS results in a multiple socket phenotype.

Expression of NECN.Scer\UAS under the control of Scer\GAL4dpp.3KK results in a strong wing vein thickening phenotype.

In NECN.Scer\UAS; Scer\GAL4sca-109-68, bristles are transformed so that they have two sockets and no shaft, a phenotype indicative of PIIa to PIIb transformation.

Overexpression of one copy of NECN.Scer\UAS under the control of Scer\GAL4ey.PH leads to a reduction of eye-disc size. Overexpression of two copies of NECN.Scer\UAS, also under control of Scer\GAL4ey.PH, leads to a more extensive eye-disc loss.

In NECN.Scer\UAS; Scer\GAL4hs.PB embryos, the ectodermal keyhole cells fail to complete their inward movement.

NECN.Scer\UAS; Scer\GAL4pros.PMG 10% of RP2 neurons are transformed into RP2 sib neurons, 94% of dMP2 neurons are converted to vMP2 and 97% of hemi-segments exhibit an increase in U neurons.

Expression of NECN.Scer\UAS under the control of Scer\GAL4ey.PH results in adults with small eyes or no eyes. Expression of NECN.Scer\UAS under the control of Scer\GAL4GMR.PF results in rough eyes which are relatively normal in size.

Expression of NECN.Scer\UAS under the control of Scer\GAL4C96 results in ragged wings and accompanying bristle loss. Expression of NECN.Scer\UAS under the control of Scer\GAL4hs.2sev results in a very mild rough eye phenotype with very low penetrance. Expression of NECN.Scer\UAS under the control of Scer\GAL4GMR.PF results in a rough eye phenotype and excess bristle formation. Animals expressing NECN.Scer\UAS under the control of Scer\GAL4pnr-MD237 show loss of microchaetae on the thorax and multiplication of macrochaetae. Animals expressing NECN.Scer\UAS under the control of Scer\GAL4A307 have a markedly impaired startle response to proprionic acid vapour compared to controls.

When expression is driven by Scer\GAL4sca-109-68 flies show a balding phenotype due to loss of socket and shaft cells.

NECN.Scer\UAS when driven by Scer\GAL4F442A.αTub84B.T:Hsim\VP16 suppresses neuroblast segregation in the embryo.

Pupae in which NECN.Scer\UAS is expressed under the control of Scer\GAL4hs.PB using heat shock at 37oC for 2 hours during the tormogen-trichogen cell fate decision show socket-to-shaft cell fate transformations.

Expression of NECN.Scer\UAS under the control of Scer\GAL4ato.3.6 has no effect on the formation of the larval or adult axonal patterns.

Expression of NECN.Scer\UAS under the control of Scer\GAL4ptc-559.1 results in an ectopic fold in the wing pouch along the anterior/posterior boundary. Expression of NECN.Scer\UAS under the control of Scer\GAL4ptc-559.1 results in a nick at the wing margin between veins 3 and 4.

Expression of NECN.Scer\UAS under the control of Scer\GAL4klu-G410 produces shortened legs due to the loss of interjoint tissue. The leg joints are still present in these flies but are sometimes fused.

Expression of NECN.Scer\UAS at 17oC during larval development throughout the wing by Scer\GAL4sd-SG29.1 disrupts its development. Cell death is concentrated around the margin region of the wing disc. The wing pouch is smaller than wild-type. Wings in these adult flies are almost completely missing wing margin. Expression of NECN.Scer\UAS at 17oC driven by Scer\GAL4sd-SG29.1 causes a reduction of the number of SOPs (sensory organ precursors) seen in the wing disc.

Expression of NECN.Scer\UAS driven by Scer\GAL4ptc-559.1 at 25oC results in a loss of scutellar bristles.

Flies expressing NECN.Scer\UAS under the control of Scer\GAL4salm-459.2 develop symmetrical ommatidia with the shape expected for ommatidia containing two R3 photoreceptor cells (more than 61% of ommatidia have this phenotype).

When NECN.Scer\UAS is expressed in embryos under the control of Scer\GAL4btl.PS, the number of esg-expressing cells at the tip of the dorsal branch in the tracheal system is increased up to four.

When expression is driven by Scer\GAL4ey.PH, the eye is very much reduced, or missing altogether.

Flies expressing NECN.Scer\UAS under the control of Scer\GAL4l(3)31-1-31-1 do not show any bristle defects.

Larvae expressing NECN.Scer\UAS under the control of Scer\GAL4klu-G410 lack the anterior spiracle.

Flies expressing NECN.Scer\UAS under the control of Scer\GAL4dpp.blk1 show disruption of the joints between tarsal segments, resulting in shortened legs.

Scer\GAL4sd-SG29.1- or Scer\GAL4Bx-MS1096-mediated expression causes loss of the majority of wing tissue.

Clones in the wing are frequently associated with nicks in the wing margin and cause the differentiation of thicker veins.

Scer\GAL4ptc-559.1-mediated expression causes a nick in the distal wing margin and a thickening of the third wing vein.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference

NECN.UAS/Scer\GAL4sca-109-68 is a suppressor of macrochaeta & mesothoracic tergum | somatic clone phenotype of Sec153

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of presutural bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior notopleural bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior notopleural bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior supraalar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior postalar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior postalar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior dorsocentral bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior scutellar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NECN.UAS, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior scutellar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

NOT Suppressor of
Other
Statement
Reference

NECN.UAS, Scer\GAL4sd-SG29.1, armS10.UAS.Tag:MYC has dorsal mesothoracic disc & sensory mother cell phenotype

NECN.UAS, Scer\GAL4sd-SG29.1, armS10.UAS.Tag:MYC has sensory mother cell & dorsal mesothoracic disc phenotype

NECN.UAS, Scer\GAL4sd-SG29.1, armUAS.cSa has sensory mother cell & dorsal mesothoracic disc phenotype

Additional Comments
Genetic Interactions
Statement
Reference

The number of medulla axons in animals co-expressing both bskDN.Scer\UAS and NECN.Scer\UAS under the control of Scer\GAL4ato.3.6 is similar to that seen in animals expressing bskDN.Scer\UAS alone under the control of Scer\GAL4ato.3.6.

The number of medulla axons in animals co-expressing both PakKK101874 and NECN.Scer\UAS under the control of Scer\GAL4ato.3.6 is indistinguishable from seen in animals expressing PakKK101874 alone under the control of Scer\GAL4ato.3.6.

Expression of NECN.Scer\UAS in the eye under the control of Scer\GAL4LL54 completely suppresses the interommatidial cell death phenotype observed in Chc4 mutant flies.

Scer\GAL4sca-109-68-mediated expression of NECN.Scer\UAS in Arp383F clones, as in a wild type background, results in a multiple socket phenotype.

Expression of NECN.Scer\UAS under the control of Scer\GAL4αTub84B.PC in ap56f/ap56f wing discs rescues wing growth.

Scer\GAL4ey.PH-mediated expression of eygScer\UAS.lune fully rescues the defective eye phenotype of mutants expressing two copies of NECN.Scer\UAS, also under the control of Scer\GAL4ey.PH. Expression of eygScer\UAS.lune fully and consistently rescues the eye-size defect in NECN.Scer\UAS mutants, where expression is driven by Scer\GAL4ey.PH. Expression of toeScer\UAS.cAa, under control of Scer\GAL4ey.PH, partially rescues the eye-size defect of NECN.Scer\UAS mutants.

Co-expression of NECN.Scer\UAS and edΔECD.Scer\UAS under the control of Scer\GAL4sca-C253 results in loss of most microchaetae on the notum and either loss of macrochaetae or tufts of bristles in their place. Clusters of neurons appear at the sites of the developing sensory organs in pupal notum.

The transformation of neurons (RP2 to RP2sib; dMP2 to vMP2; Usib to U) seen in NECN.Scer\UAS; Scer\GAL4pros.PMG embryos is unaffected by spdoG104/spdoG104.

The reduced eye phenotype caused by expression of NECN.Scer\UAS under the control of Scer\GAL4ey.PH is partially suppressed in the ventral part of the eye by coexpression of CG46146Scer\UAS.cCa. The rough eye phenotype caused by expression of NECN.Scer\UAS under the control of Scer\GAL4GMR.PF is not suppressed by coexpression of CG46146Scer\UAS.cCa.

NECN.Scer\UAS (Scer\GAL4sca-109-68), msi1 double mutants show a dense double bristle phenotype. The IIb precursor takes the non-neuronal fate as there are no neurons in the subepidermal layer.

In PsnC2 wing disc clones that also express NECN.Scer\UAS (under the control of Scer\GAL4F442A.αTub84B.T:Hsim\VP16) formation of multiple sensory organ mother cell (SMCs) is seen in place of single SMCs.

PsnC2 clones that also express NECN.Scer\UAS, give adult wing phenotypes: clones that cross the wing margin lead to wing notching, whereas clones within the dorsal compartment of the blade can form abnormally thick veins. If PsnαTub84B.PS is also added, a double dorsal wing outgrowth develops, flanked by adjacent rows of dorsal wing margin bristles.

In PsnC2 clones that also express NECN.Scer\UAS (under the control of Scer\GAL4F442A.αTub84B.T:Hsim\VP16) formation of multiple sensory organ mother cell (SMCs) is seen in place of single SMCs. PsnC2 clones that also express NECN.Scer\UAS, give wing phenotypes. Clones that cross the wing margin lead to wing notching. Clones within the dorsal compartment of the blade can form abnormally thick veins. If PsnαTub84B.PS is also added, a double dorsal wing outgrowth develops, flanked by adjacent rows of dorsal wing margin bristles.

The wing phenotype caused by expression of SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 is suppressed by coexpression of NECN.Scer\UAS. The ectopic margin caused by expression of DlScer\UAS.cHa under the control of Scer\GAL4ptc-559.1 is suppressed by coexpression of NECN.Scer\UAS, except for a small patch on the dorsal side, and the wing is almost wild type in shape.

The addition of wgScer\UAS.cGa suppresses the wing pouch phenotype seen in NECN.Scer\UAS/Scer\GAL4sd-SG29.1 wing discs and leads to cell death occurring over the whole of the wing pouch. Wings in these adult flies are small and bear a small number of bristles. The addition of wgl-12.Scer\UAS suppresses the wing pouch phenotype seen in NECN.Scer\UAS/Scer\GAL4sd-SG29.1 wing discs, and abolishes the cell death phenotype. Wings in these adult flies are almost wild-type in size and in many instances display a partial restoration of bristles at the edge. In addition these wings display enlarged veins which are much broader than those produced by either NECN.Scer\UAS or wgl-12.Scer\UAS alone. The addition of armS10.Scer\UAS.T:Hsap\MYC to NECN.Scer\UAS/Scer\GAL4sd-SG29.1 flies have smaller wing pouches, In the adult wing they show a combination of the phenotypes seen in flies expressing NECN.Scer\UAS and armS10.Scer\UAS.T:Hsap\MYC alone i.e. the adults wings are small, lacking a margin and are covered in bristles. The addition of wgl-12.Scer\UAS has no effect on the sensory organ precursor phenotype of animals expressing NECN.Scer\UAS driven by Scer\GAL4sd-SG29.1at 17oC. The addition of armS10.Scer\UAS.T:Hsap\MYC leads to an increase in the area covered by SOPs, though they are not evenly spaced. The addition of wgScer\UAS.cGa leads to an increase in the area covered by SOPs, similar to when wgScer\UAS.cGa is expressed alone, though they are not evenly spaced.

Coexpression of NECN.Scer\UAS completely suppresses the transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by DlScer\UAS.cJa expressed under the control of Scer\GAL4l(3)31-1-31-1.

Coexpression of NECN.Scer\UAS and SerScer\UAS.cSa under the control of Scer\GAL4sd-SG29.1 demonstrates Ser can partially suppress the effect of N : wings are smaller than wild type, the dorsal surface is smaller than the ventral surface.

Scer\GAL4ptc-559.1-mediated coexpression with SerScer\UAS.cSa allows most flies to hatch at 25oC, both phenotypes provoked by SerScer\UAS.cSa alone (extra wing tissue on the ventral side and formation of a notch in the margin) are strongly suppressed.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
NECN.Scer\UAS
NECN.UAS
Necd.UAS
Name Synonyms
Secondary FlyBase IDs
  • FBal0066008
References (54)