Allele Dmel\N^ECN.Scer\UAS

General Information
Symbol	Dmel\N^ECN.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0058505
Feature type	allele	Associated gene	Dmel\N

Allele class	antimorphic allele - genetic evidence
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	antimorphic allele - genetic evidence (Dominguez et al., 2004)
Mutagen	in vitro construct - regulatory fusion (Jonsson and Knust, 1996, Klein and Martinez Arias, 1998, Jacobsen et al., 1998, de Celis et al., 1998, Brennan et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A 5.7kb NruI fragment from a N cDNA is expressed under the control of Scer\UAS sequences. (Brennan et al., 1999) Scer\UAS regulatory sequences drive amino acids 1 to 1789 of N (this encodes a protein which lacks most of the intracellular domain). (Jacobsen et al., 1998) Expression of the N extracellular domain is governed by Scer\UAS regulatory sequences. (de Celis and Bray, 1997) Construct: Scer\GAL4 Scer\UAS binding govern expression of a N cDNA encoding a membrane bound extracellular domain of N, which lacks most of the cytoplasmic domain. (Jonsson and Knust, 1996)
Carried in construct	P{UAS-N.ECN} (Acar et al., 2008, Chern and Choi, 2002, Brennan et al., 1999, Brennan et al., 1999, Dobens et al., 2005, Zeidler et al., 1999, Chung and Struhl, 2001, Wang and Struhl, 2005, Goto et al., 2001, Presente et al., 2002, Dominguez et al., 2004, Fuss et al., 2004, Chihara and Hayashi, 2000, Escudero et al., 2003, Struhl and Greenwald, 2001, de Celis and Bray, 2000, Lawrence et al., 2000, Barolo et al., 2000, Hassan et al., 2000, Baonza et al., 2000, Cavodeassi et al., 1999, Cooper and Bray, 1999, de Celis et al., 1998, Jacobsen et al., 1998, Bishop et al., 1999, Klein et al., 1997, de Celis and Bray, 1997, Dominguez and de Celis, 1998, Klein and Martinez Arias, 1998, Okabe et al., 2001, Hao et al., 2003, Klein, 2003, Kenyon et al., 2003, Jonsson and Knust, 1996, O'Connor-Giles and Skeath, 2003, Suzanne et al., 2003, Ikeya and Hayashi, 1999, Molnar et al., 2006, Jafar-Nejad et al., 2005, Zecca and Struhl, 2007, Xie et al., 2012, Rajan et al., 2009, Pueyo and Couso, 2011, Tien et al., 2008, Yan et al., 2009, Babaoglan et al., 2009, Bayraktar et al., 2006, Peralta et al., 2009, Lee et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	chemosensitive behavior defective, with Scer\GAL4^A307 (Presente et al., 2002) increased cell death, with Scer\GAL4^LL54 (Peralta et al., 2009) planar polarity defective, with Scer\GAL4^salm-459.2 (Cooper and Bray, 1999) visible, with Scer\GAL4^bbg-C96 (Presente et al., 2002) visible, with Scer\GAL4^bi-omb-Gal4 (Pueyo and Couso, 2011) visible, with Scer\GAL4^dpp.3KK (Molnar et al., 2006) visible, with Scer\GAL4^dpp.blk1 (de Celis et al., 1998) visible, with Scer\GAL4^ey.PH (Chern and Choi, 2002) visible, with Scer\GAL4^GMR.PF (Presente et al., 2002, Chern and Choi, 2002) visible, with Scer\GAL4^hs.2sev (Presente et al., 2002) visible, with Scer\GAL4^hs.PB (Barolo et al., 2000) visible, with Scer\GAL4^klu-G410 (Bishop et al., 1999) visible, with Scer\GAL4^pnr-MD237 (Presente et al., 2002) visible, with Scer\GAL4^ptc-559.1 (Brennan et al., 1999, Lawrence et al., 2000) visible, with Scer\GAL4^sca-109-68 (Okabe et al., 2001) visible, with Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) visible, with Scer\GAL4^Ubx.PdC (de Celis and Bray, 1997)
Phenotype Manifest In
	adult thorax & microchaeta, with Scer\GAL4^pnr-MD237 (Presente et al., 2002) dMP2 neuron, with Scer\GAL4^pros.PMG (O'Connor-Giles and Skeath, 2003) EL neuron, with Scer\GAL4^pros.PMG (O'Connor-Giles and Skeath, 2003) embryonic/larval anterior spiracle, with Scer\GAL4^klu-G410 (Klein and Martinez Arias, 1998) embryonic/larval dorsal branch, with Scer\GAL4^btl.PS (Ikeya and Hayashi, 1999) embryonic/larval dorsal branch & tracheal tip cell, with Scer\GAL4^btl.PS (Ikeya and Hayashi, 1999) embryonic heart cardioblast \| embryonic stage 16, with Scer\GAL4^twi.PG (Babaoglan et al., 2009) embryonic neuroblast, with Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} (Struhl and Greenwald, 2001) embryonic pericardial cell \| embryonic stage 12, with Scer\GAL4^twi.PG (Babaoglan et al., 2009) embryonic pericardial cell \| embryonic stage 15, with Scer\GAL4^twi.PG (Babaoglan et al., 2009) embryonic pericardial cell \| embryonic stage 16, with Scer\GAL4^twi.PG (Babaoglan et al., 2009) embryonic proventriculus, with Scer\GAL4^hs.PB (Fuss et al., 2004) external sensory organ, with Scer\GAL4^sca-109-68 (Okabe et al., 2001) eye, with Scer\GAL4^ey.PH (Chern and Choi, 2002, Dominguez and de Celis, 1998) eye, with Scer\GAL4^GMR.PF (Presente et al., 2002, Chern and Choi, 2002) eye disc, with Scer\GAL4^ey.PH (Dominguez et al., 2004) joint, with Scer\GAL4^bi-omb-Gal4 (Pueyo and Couso, 2011) keyhole structure, with Scer\GAL4^hs.PB (Fuss et al., 2004) leg, with Scer\GAL4^bi-omb-Gal4 (Pueyo and Couso, 2011) leg, with Scer\GAL4^dpp.blk1 (de Celis et al., 1998) leg, with Scer\GAL4^klu-G410 (Bishop et al., 1999) leg & joint, with Scer\GAL4^klu-G410 (Bishop et al., 1999) macrochaeta, with Scer\GAL4^ptc-559.1 (Brennan et al., 1999) macrochaeta & mesothoracic tergum, with Scer\GAL4^sca-109-68 (Jafar-Nejad et al., 2005) macrochaeta \| supernumerary, with Scer\GAL4^pnr-MD237 (Presente et al., 2002) ommatidium, with Scer\GAL4^GMR.PF (Presente et al., 2002, Chern and Choi, 2002) ommatidium, with Scer\GAL4^hs.2sev (Presente et al., 2002) ommatidium, with Scer\GAL4^salm-459.2 (Cooper and Bray, 1999) photoreceptor cell R4, with Scer\GAL4^salm-459.2 (Cooper and Bray, 1999) proventriculus inner layer primordium, with Scer\GAL4^hs.PB (Fuss et al., 2004) proventriculus intermediate layer primordium, with Scer\GAL4^hs.PB (Fuss et al., 2004) RP2 motor neuron, with Scer\GAL4^pros.PMG (O'Connor-Giles and Skeath, 2003) RP2sib neuron \| supernumerary, with Scer\GAL4^pros.PMG (O'Connor-Giles and Skeath, 2003) secondary pigment cell, with Scer\GAL4^LL54 (Peralta et al., 2009) sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) tarsal segment, with Scer\GAL4^dpp.blk1 (de Celis et al., 1998) tertiary pigment cell, with Scer\GAL4^LL54 (Peralta et al., 2009) tormogen cell, with Scer\GAL4^hs.PB (Barolo et al., 2000) tormogen cell, with Scer\GAL4^sca-109-68 (Okabe et al., 2001) tormogen cell & mesothoracic tergum \| supernumerary, with Scer\GAL4^sca-109-68 (Jafar-Nejad et al., 2005) tormogen cell \| supernumerary, with Scer\GAL4^sca-109-68 (Rajan et al., 2009) trichogen cell, with Scer\GAL4^sca-109-68 (Okabe et al., 2001) trichogen cell \| ectopic, with Scer\GAL4^hs.PB (Barolo et al., 2000) U neuron \| supernumerary, with Scer\GAL4^pros.PMG (O'Connor-Giles and Skeath, 2003) Usib neuron, with Scer\GAL4^pros.PMG (O'Connor-Giles and Skeath, 2003) vMP2 neuron \| supernumerary, with Scer\GAL4^pros.PMG (O'Connor-Giles and Skeath, 2003) wing, with Scer\GAL4^bbg-C96 (Presente et al., 2002) wing, with Scer\GAL4^Bx-MS1096 (Klein et al., 1997) wing, with Scer\GAL4^sd-SG29.1 (Brennan et al., 1999, Klein et al., 1997) wing, with Scer\GAL4^Ubx.PdC (de Celis and Bray, 1997) wing disc, with Scer\GAL4^ptc-559.1 (Jonsson and Knust, 1996, Lawrence et al., 2000) wing disc, with Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) wing margin, with Scer\GAL4^ptc-559.1 (Lawrence et al., 2000) wing margin bristle, with Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) wing vein, with Scer\GAL4^dpp.3KK (Molnar et al., 2006) wing vein, with Scer\GAL4^Ubx.PdC (de Celis and Bray, 1997)
Detailed Description
	Statement Reference Males expressing N[ECN.Scer\UAS] under the control of Scer\GAL4[Abd-B-LDN] show normal genitalia rotation. (Lee et al., 2011) Expression of N[ECN.Scer\UAS] under the control of Scer\GAL4[bi-omb-Gal4] results in deformed legs with incomplete joints. (Pueyo and Couso, 2011) Expression of N[ECN.Scer\UAS] under the control of Scer\GAL4[twi.PG] leads to a near complete loss of svp-positive heart precursors and heart cells. eve-positive equivalence groups form when N[ECN.Scer\UAS] is driven under the control of Scer\GAL4[twi.PG], however few precursors and eve-positive pericardial cells are seen in this background. (Babaoglan et al., 2009) Expression of N[ECN.Scer\UAS] in the eye under the control of Scer\GAL4[LL54] results in an increase in interommatidial pigment cell death. (Peralta et al., 2009) Scer\GAL4[sca-109-68]-mediated expression of N[ECN.Scer\UAS] results in a multiple socket phenotype. (Rajan et al., 2009) Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^dpp.3KK results in a strong wing vein thickening phenotype. (Molnar et al., 2006) In N^ECN.Scer\UAS; Scer\GAL4^sca-109-68, bristles are transformed so that they have two sockets and no shaft, a phenotype indicative of PIIa to PIIb transformation. (Jafar-Nejad et al., 2005) Overexpression of one copy of N^ECN.Scer\UAS under the control of Scer\GAL4^ey.PH leads to a reduction of eye-disc size. Overexpression of two copies of N^ECN.Scer\UAS, also under control of Scer\GAL4^ey.PH, leads to a more extensive eye-disc loss. (Dominguez et al., 2004) In N^ECN.Scer\UAS; Scer\GAL4^hs.PB embryos, the ectodermal keyhole cells fail to complete their inward movement. (Fuss et al., 2004) N^ECN.Scer\UAS; Scer\GAL4^pros.PMG 10% of RP2 neurons are transformed into RP2 sib neurons, 94% of dMP2 neurons are converted to vMP2 and 97% of hemi-segments exhibit an increase in U neurons. (O'Connor-Giles and Skeath, 2003) Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ey.PH results in adults with small eyes or no eyes. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^GMR.PF results in rough eyes which are relatively normal in size. (Chern and Choi, 2002) Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^C96 results in ragged wings and accompanying bristle loss. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^hs.2sev results in a very mild rough eye phenotype with very low penetrance. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype and excess bristle formation. Animals expressing N^ECN.Scer\UAS under the control of Scer\GAL4^pnr-MD237 show loss of microchaetae on the thorax and multiplication of macrochaetae. Animals expressing N^ECN.Scer\UAS under the control of Scer\GAL4^A307 have a markedly impaired startle response to proprionic acid vapour compared to controls. (Presente et al., 2002) When expression is driven by Scer\GAL4^sca-109-68 flies show a balding phenotype due to loss of socket and shaft cells. (Okabe et al., 2001) N^ECN.Scer\UAS when driven by Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} suppresses neuroblast segregation in the embryo. (Struhl and Greenwald, 2001) Pupae in which N^ECN.Scer\UAS is expressed under the control of Scer\GAL4^hs.PB using heat shock at 37^oC for 2 hours during the tormogen-trichogen cell fate decision show socket-to-shaft cell fate transformations. (Barolo et al., 2000) Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ato.3.6 has no effect on the formation of the larval or adult axonal patterns. (Hassan et al., 2000) Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ptc-559.1 results in an ectopic fold in the wing pouch along the anterior/posterior boundary. Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ptc-559.1 results in a nick at the wing margin between veins 3 and 4. (Lawrence et al., 2000) Expression of N^ECN.Scer\UAS under the control of Scer\GAL4^klu-G410 produces shortened legs due to the loss of interjoint tissue. The leg joints are still present in these flies but are sometimes fused. (Bishop et al., 1999) Expression of N^ECN.Scer\UAS driven by Scer\GAL4^ptc-559.1 at 25^oC results in a loss of scutellar bristles. (Brennan et al., 1999) Expression of N^ECN.Scer\UAS at 17^oC during larval development throughout the wing by Scer\GAL4^sd-SG29.1 disrupts its development. Cell death is concentrated around the margin region of the wing disc. The wing pouch is smaller than wild-type. Wings in these adult flies are almost completely missing wing margin. Expression of N^ECN.Scer\UAS at 17^oC driven by Scer\GAL4^sd-SG29.1 causes a reduction of the number of SOPs (sensory organ precursors) seen in the wing disc. (Brennan et al., 1999) Flies expressing N^ECN.Scer\UAS under the control of Scer\GAL4^salm-459.2 develop symmetrical ommatidia with the shape expected for ommatidia containing two R3 photoreceptor cells (more than 61% of ommatidia have this phenotype). (Cooper and Bray, 1999) When N^ECN.Scer\UAS is expressed in embryos under the control of Scer\GAL4^btl.PS, the number of esg-expressing cells at the tip of the dorsal branch in the tracheal system is increased up to four. (Ikeya and Hayashi, 1999) Flies expressing N^ECN.Scer\UAS under the control of Scer\GAL4^dpp.blk1 show disruption of the joints between tarsal segments, resulting in shortened legs. (de Celis et al., 1998) When expression is driven by Scer\GAL4^ey.PH, the eye is very much reduced, or missing altogether. (Dominguez and de Celis, 1998) Flies expressing N^ECN.Scer\UAS under the control of Scer\GAL4^{l(3)31-1-31-1} do not show any bristle defects. (Jacobsen et al., 1998) Larvae expressing N^ECN.Scer\UAS under the control of Scer\GAL4^klu-G410 lack the anterior spiracle. (Klein and Martinez Arias, 1998) Clones in the wing are frequently associated with nicks in the wing margin and cause the differentiation of thicker veins. (de Celis and Bray, 1997) Scer\GAL4^sd-SG29.1- or Scer\GAL4^Bx-MS1096-mediated expression causes loss of the majority of wing tissue. (Klein et al., 1997) Scer\GAL4^ptc-559.1-mediated expression causes a nick in the distal wing margin and a thickening of the third wing vein. (Jonsson and Knust, 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^ey.PH has visible phenotype, suppressible \| partially by L^Scer\UAS.cCa, Scer\GAL4^ey.PH (Chern and Choi, 2002) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1 has visible phenotype, suppressible by wg^{l-12.Scer\UAS}, Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1 has visible phenotype, suppressible by wg^Scer\UAS.cGa, Scer\GAL4^sd-SG29.1 (Brennan et al., 1999)
NOT suppressed by
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by L^Scer\UAS.cCa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chern and Choi, 2002)
Suppressor of
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of visible phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^ptc-559.1 is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Ser^Scer\UAS.cSa (Lawrence et al., 2000) N^ECN.Scer\UAS/Scer\GAL4^LL54 is a suppressor of increased cell death phenotype of Chc⁴ (Peralta et al., 2009) Scer\GAL4^ptc-559.1/N^ECN.Scer\UAS is a suppressor \| partially of visible phenotype of Dl^Scer\UAS.cHa (Lawrence et al., 2000)
NOT Suppressor of
Statement Reference N^ECN.Scer\UAS/Scer\GAL4^sca-109-68 is a non-suppressor of visible \| recessive phenotype of msi¹ (Okabe et al., 2001)
Other
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^sca-109-68, msi¹ has visible phenotype (Okabe et al., 2001, Okabe et al., 2001) N^ECN.Scer\UAS, Scer\GAL4^sca-C253, ed^{ΔECD.Scer\UAS} has visible phenotype (Escudero et al., 2003, Escudero et al., 2003)
Phenotype Manifest In
NOT Enhanced by
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has dMP2 neuron phenotype, non-enhanceable by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has EL neuron phenotype, non-enhanceable by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has RP2 motor neuron phenotype, non-enhanceable by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has RP2sib neuron \| supernumerary phenotype, non-enhanceable by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has U neuron \| supernumerary phenotype, non-enhanceable by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has Usib neuron phenotype, non-enhanceable by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has vMP2 neuron \| supernumerary phenotype, non-enhanceable by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003)
Suppressed by
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^ey.PH has eye \| ventral phenotype, suppressible \| partially by L^Scer\UAS.cCa, Scer\GAL4^ey.PH (Chern and Choi, 2002) N^ECN.Scer\UAS, Scer\GAL4^ey.PH has eye disc phenotype, suppressible by eyg^{Scer\UAS.lune}, Scer\GAL4^ey.PH (Dominguez et al., 2004) N^ECN.Scer\UAS, Scer\GAL4^ey.PH has eye phenotype, suppressible \| partially by toe^Scer\UAS.cAa, Scer\GAL4^ey.PH (Dominguez et al., 2004) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1 has wing disc phenotype, suppressible by wg^Scer\UAS.cGa, Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible by wg^Scer\UAS.cGa, Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1 has wing vein phenotype, suppressible by wg^{l-12.Scer\UAS}, Scer\GAL4^sd-SG29.1 (Brennan et al., 1999)
NOT suppressed by
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by L^Scer\UAS.cCa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chern and Choi, 2002) N^ECN.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by L^Scer\UAS.cCa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chern and Choi, 2002) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has dMP2 neuron phenotype, non-suppressible by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has EL neuron phenotype, non-suppressible by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has RP2 motor neuron phenotype, non-suppressible by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has RP2sib neuron \| supernumerary phenotype, non-suppressible by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has U neuron \| supernumerary phenotype, non-suppressible by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has Usib neuron phenotype, non-suppressible by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003) N^ECN.Scer\UAS, Scer\GAL4^pros.PMG has vMP2 neuron \| supernumerary phenotype, non-suppressible by spdo^G104/spdo^G104 (O'Connor-Giles and Skeath, 2003)
Suppressor of
Statement Reference N^ECN.Scer\UAS, Scer\GAL4^Dll-md23 is a suppressor of eye \| ectopic phenotype of Scer\GAL4^Dll-md23, danr^Scer\UAS.cSa (Suzanne et al., 2003) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior notopleural bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior postalar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior scutellar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior supraalar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior dorsocentral bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior notopleural bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior postalar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior scutellar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of presutural bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) N^ECN.Scer\UAS, Scer\GAL4^ptc-559.1 is a suppressor of wing margin \| ectopic phenotype of Scer\GAL4^ptc-559.1, Ser^Scer\UAS.cSa (Lawrence et al., 2000) N^ECN.Scer\UAS, Scer\GAL4^ptc-559.1 is a suppressor of wing phenotype of Scer\GAL4^ptc-559.1, Ser^Scer\UAS.cSa (Lawrence et al., 2000) N^ECN.Scer\UAS/Scer\GAL4^LL54 is a suppressor of secondary pigment cell phenotype of Chc⁴ (Peralta et al., 2009) N^ECN.Scer\UAS/Scer\GAL4^LL54 is a suppressor of tertiary pigment cell phenotype of Chc⁴ (Peralta et al., 2009) N^ECN.Scer\UAS/Scer\GAL4^sca-109-68 is a suppressor of macrochaeta & mesothoracic tergum \| somatic clone phenotype of sec15³ (Jafar-Nejad et al., 2005) N^ECN.Scer\UAS/Scer\GAL4^αTub84B.PC is a suppressor of wing disc phenotype of ap^56f (Zecca and Struhl, 2007) Scer\GAL4^ptc-559.1/N^ECN.Scer\UAS is a suppressor \| partially of wing margin \| ectopic phenotype of Dl^Scer\UAS.cHa (Lawrence et al., 2000) Scer\GAL4^ptc-559.1/N^ECN.Scer\UAS is a suppressor \| partially of wing phenotype of Dl^Scer\UAS.cHa (Lawrence et al., 2000)
NOT Suppressor of
Statement Reference N^ECN.Scer\UAS/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21 (Lee et al., 2011) N^ECN.Scer\UAS/Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} is a non-suppressor of embryonic neuroblast \| supernumerary phenotype of Psn^C2 (Struhl and Greenwald, 2001) N^ECN.Scer\UAS/Scer\GAL4^sca-109-68 is a non-suppressor of external sensory organ phenotype of msi¹ (Okabe et al., 2001) N^ECN.Scer\UAS/Scer\GAL4^sca-109-68 is a non-suppressor of trichogen cell \| supernumerary phenotype of msi¹ (Okabe et al., 2001)
Other
Statement Reference N^ECN.Scer\UAS, Psn^C2, Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} has sensory mother cell \| supernumerary phenotype (Struhl and Greenwald, 2001, Struhl and Greenwald, 2001) N^ECN.Scer\UAS, Psn^C2, Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} has wing margin phenotype (Struhl and Greenwald, 2001, Struhl and Greenwald, 2001) N^ECN.Scer\UAS, Psn^C2, Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} has wing vein phenotype (Struhl and Greenwald, 2001, Struhl and Greenwald, 2001) N^ECN.Scer\UAS, Psn^αTub84B.PS, Psn^C2, Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} has wing \| ectopic phenotype (Struhl and Greenwald, 2001, Struhl and Greenwald, 2001) N^ECN.Scer\UAS, Scer\GAL4^sca-109-68, msi¹ has eo neuron phenotype (Okabe et al., 2001, Okabe et al., 2001) N^ECN.Scer\UAS, Scer\GAL4^sca-109-68, msi¹ has external sensory organ phenotype (Okabe et al., 2001, Okabe et al., 2001) N^ECN.Scer\UAS, Scer\GAL4^sca-109-68, msi¹ has trichogen cell phenotype (Okabe et al., 2001, Okabe et al., 2001) N^ECN.Scer\UAS, Scer\GAL4^sca-109-68 has tormogen cell \| supernumerary phenotype (Rajan et al., 2009) N^ECN.Scer\UAS, Scer\GAL4^sca-C253, ed^{ΔECD.Scer\UAS} has macrochaeta \| ectopic phenotype (Escudero et al., 2003, Escudero et al., 2003) N^ECN.Scer\UAS, Scer\GAL4^sca-C253, ed^{ΔECD.Scer\UAS} has macrochaeta phenotype (Escudero et al., 2003, Escudero et al., 2003) N^ECN.Scer\UAS, Scer\GAL4^sca-C253, ed^{ΔECD.Scer\UAS} has microchaeta phenotype (Escudero et al., 2003, Escudero et al., 2003) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1, arm^{S10.Scer\UAS.T:Hsap\MYC} has dorsal mesothoracic disc & sensory mother cell phenotype (Brennan et al., 1999) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1, arm^{S10.Scer\UAS.T:Hsap\MYC} has sensory mother cell & dorsal mesothoracic disc phenotype (Brennan et al., 1999) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1, arm^{S10.Scer\UAS.T:Hsap\MYC} has wing disc phenotype (Brennan et al., 1999, Brennan et al., 1999) N^ECN.Scer\UAS, Scer\GAL4^sd-SG29.1, arm^Scer\UAS.cSa has sensory mother cell & dorsal mesothoracic disc phenotype (Brennan et al., 1999) N^ECN.Scer\UAS, Scer\GAL4^unspecified, nct^R46 has sensory mother cell \| ectopic phenotype (Chung and Struhl, 2001, Chung and Struhl, 2001) N^ECN.Scer\UAS, Scer\GAL4^unspecified, nct^R46 has wing hinge phenotype (Chung and Struhl, 2001, Chung and Struhl, 2001)
Additional Comments
Genetic Interactions
Statement Reference Expression of N[ECN.Scer\UAS] in the eye under the control of Scer\GAL4[LL54] completely suppresses the interommatidial cell death phenotype observed in Chc[4] mutant flies. (Peralta et al., 2009) Scer\GAL4[sca-109-68]-mediated expression of N[ECN.Scer\UAS] in Arp3[83F] clones, as in a wild type background, results in a multiple socket phenotype. (Rajan et al., 2009) Expression of N[ECN.Scer\UAS] under the control of Scer\GAL4[αTub84B.PC] in ap[56f]/ap[56f] wing discs rescues wing growth. (Zecca and Struhl, 2007) Scer\GAL4^ey.PH-mediated expression of eyg^{Scer\UAS.lune} fully rescues the defective eye phenotype of mutants expressing two copies of N^ECN.Scer\UAS, also under the control of Scer\GAL4^ey.PH. Expression of eyg^{Scer\UAS.lune} fully and consistently rescues the eye-size defect in N^ECN.Scer\UAS mutants, where expression is driven by Scer\GAL4^ey.PH. Expression of toe^Scer\UAS.cAa, under control of Scer\GAL4^ey.PH, partially rescues the eye-size defect of N^ECN.Scer\UAS mutants. (Dominguez et al., 2004) Co-expression of N^ECN.Scer\UAS and ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-C253 results in loss of most microchaetae on the notum and either loss of macrochaetae or tufts of bristles in their place. Clusters of neurons appear at the sites of the developing sensory organs in pupal notum. (Escudero et al., 2003) The transformation of neurons (RP2 to RP2sib; dMP2 to vMP2; Usib to U) seen in N^ECN.Scer\UAS; Scer\GAL4^pros.PMG embryos is unaffected by spdo^G104/spdo^G104. (O'Connor-Giles and Skeath, 2003) The reduced eye phenotype caused by expression of N^ECN.Scer\UAS under the control of Scer\GAL4^ey.PH is partially suppressed in the ventral part of the eye by coexpression of L^Scer\UAS.cCa. The rough eye phenotype caused by expression of N^ECN.Scer\UAS under the control of Scer\GAL4^GMR.PF is not suppressed by coexpression of L^Scer\UAS.cCa. (Chern and Choi, 2002) N^ECN.Scer\UAS (Scer\GAL4^sca-109-68), msi¹ double mutants show a dense double bristle phenotype. The IIb precursor takes the non-neuronal fate as there are no neurons in the subepidermal layer. (Okabe et al., 2001) In Psn^C2 clones that also express N^ECN.Scer\UAS (under the control of Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16}) formation of multiple sensory organ mother cell (SMCs) is seen in place of single SMCs. Psn^C2 clones that also express N^ECN.Scer\UAS, give wing phenotypes. Clones that cross the wing margin lead to wing notching. Clones within the dorsal compartment of the blade can form abnormally thick veins. If Psn^αTub84B.PS is also added, a double dorsal wing outgrowth develops, flanked by adjacent rows of dorsal wing margin bristles. (Struhl and Greenwald, 2001) In Psn^C2 wing disc clones that also express N^ECN.Scer\UAS (under the control of Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16}) formation of multiple sensory organ mother cell (SMCs) is seen in place of single SMCs. Psn^C2 clones that also express N^ECN.Scer\UAS, give adult wing phenotypes: clones that cross the wing margin lead to wing notching, whereas clones within the dorsal compartment of the blade can form abnormally thick veins. If Psn^αTub84B.PS is also added, a double dorsal wing outgrowth develops, flanked by adjacent rows of dorsal wing margin bristles. (Struhl and Greenwald, 2001) The wing phenotype caused by expression of Ser^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 is suppressed by coexpression of N^ECN.Scer\UAS. The ectopic margin caused by expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 is suppressed by coexpression of N^ECN.Scer\UAS, except for a small patch on the dorsal side, and the wing is almost wild type in shape. (Lawrence et al., 2000) The addition of wg^Scer\UAS.cGa suppresses the wing pouch phenotype seen in N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 wing discs and leads to cell death occurring over the whole of the wing pouch. Wings in these adult flies are small and bear a small number of bristles. The addition of wg^{l-12.Scer\UAS} suppresses the wing pouch phenotype seen in N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 wing discs, and abolishes the cell death phenotype. Wings in these adult flies are almost wild-type in size and in many instances display a partial restoration of bristles at the edge. In addition these wings display enlarged veins which are much broader than those produced by either N^ECN.Scer\UAS or wg^{l-12.Scer\UAS} alone. The addition of arm^{S10.Scer\UAS.T:Hsap\MYC} to N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 flies have smaller wing pouches, In the adult wing they show a combination of the phenotypes seen in flies expressing N^ECN.Scer\UAS and arm^{S10.Scer\UAS.T:Hsap\MYC} alone i.e. the adults wings are small, lacking a margin and are covered in bristles. The addition of wg^{l-12.Scer\UAS} has no effect on the sensory organ precursor phenotype of animals expressing N^ECN.Scer\UAS driven by Scer\GAL4^sd-SG29.1at 17^oC. The addition of arm^{S10.Scer\UAS.T:Hsap\MYC} leads to an increase in the area covered by SOPs, though they are not evenly spaced. The addition of wg^Scer\UAS.cGa leads to an increase in the area covered by SOPs, similar to when wg^Scer\UAS.cGa is expressed alone, though they are not evenly spaced. (Brennan et al., 1999) Coexpression of N^ECN.Scer\UAS completely suppresses the transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by Dl^Scer\UAS.cJa expressed under the control of Scer\GAL4^{l(3)31-1-31-1}. (Jacobsen et al., 1998) Coexpression of N^ECN.Scer\UAS and Ser^Scer\UAS.cSa under the control of Scer\GAL4^sd-SG29.1 demonstrates Ser can partially suppress the effect of N: wings are smaller than wild type, the dorsal surface is smaller than the ventral surface. (Klein et al., 1997) Scer\GAL4^ptc-559.1-mediated coexpression with Ser^Scer\UAS.cSa allows most flies to hatch at 25^oC, both phenotypes provoked by Ser^Scer\UAS.cSa alone (extra wing tissue on the ventral side and formation of a notch in the margin) are strongly suppressed. (Jonsson and Knust, 1996)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	N^ECN.Scer\UAS is partially rescued by Scer\GAL4^sd-SG29.1/N^{int.I.Scer\UAS} (Brennan et al., 1999)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	M. Baylies.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	N^dn (Cooper and Bray, 1999) N^ecd.UAS N^ECN.Scer\UAS N^ECN.UAS
Name Synonym
Secondary FlyBase IDs
	FBal0066008
References ( 49 )

Generate a list of	All references relating to this allele ( 49 )

List References by type	Research paper ( 49 )
Recent research papers ( 3 )
	Xie et al., 2012, PLoS ONE 7(4): e36362 Uif, a Large Transmembrane Protein with EGF-Like Repeats, Can Antagonize Notch Signaling in Drosophila. [FBrf0218242] Lee et al., 2011, Dev. Biol. 356(2): 588--597 UVRAG is required for organ rotation by regulating Notch endocytosis in Drosophila. [FBrf0214470] Pueyo and Couso, 2011, Dev. Biol. 355(2): 183--193 Tarsal-less peptides control Notch signalling through the Shavenbaby transcription factor. [FBrf0213970] Show all research papers ...

Allele Dmel\NECN.Scer\UAS

Allele Dmel\N^ECN.Scer\UAS