FlyBase Allele Report: Scer\GAL4[c309]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^c309

General Information

Symbol

Scer\GAL4^c309

Species

S. cerevisiae

Name

FlyBase ID

FBal0058765

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

C309, c309-GAL4, P{GawB}c309

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Koushika et al., 1996)

Progenitor genotype

Associated Insertion(s)

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval ventral nerve cord

(Pfeiffenberger and Allada, 2012)

embryonic/larval mushroom body

(Kester and Nambu, 2011)

adult ventral nerve cord

(Kester and Nambu, 2011)

eye-antennal disc

(Kester and Nambu, 2011)

wandering third instar larval stage

antennal disc | restricted

(Manseau et al., 1997)

(Manseau et al., 1997)

eye disc | posterior

(Manseau et al., 1997)

adult brain | restricted

(Connolly et al., 1996)

adult ventral nerve cord

(Connolly et al., 1996)

adult mushroom body

(Pfeiffenberger and Allada, 2012, Dubnau et al., 2001, Waddell et al., 2000, Connolly et al., 1996)

adult mushroom body | restricted

(Bushey et al., 2009, Zhou et al., 2008, Nitz et al., 2002)

adult mushroom body alpha-lobe

(Krashes et al., 2007)

adult mushroom body beta-lobe

(Krashes et al., 2007)

(Pfeiffenberger and Allada, 2012)

gamma Kenyon cell

(Pauls et al., 2010)

alpha/beta Kenyon cell

(Pauls et al., 2010)

alpha'/beta' Kenyon cell

(Pauls et al., 2010)

adult mushroom body gamma-lobe

(Pauls et al., 2010, Aso et al., 2009, Krashes et al., 2007)

adult mushroom body beta'-lobe | restricted

(Krashes et al., 2007)

adult mushroom body alpha'-lobe | restricted

(Krashes et al., 2007)

mushroom body beta lobe core layer | intense

(Aso et al., 2009)

mushroom body alpha lobe core layer | intense

(Aso et al., 2009)

mushroom body beta lobe surface layer | intense

(Aso et al., 2009)

mushroom body alpha lobe surface layer | intense

(Aso et al., 2009)

mushroom body beta lobe posterior layer | intense

(Aso et al., 2009)

mushroom body alpha lobe posterior layer | intense

(Aso et al., 2009)

adult antennal lobe | faint

(Aso et al., 2009)

ellipsoid body | faint

(Aso et al., 2009)

medulla | intense

(Aso et al., 2009)

(Aso et al., 2009)

lobula plate | faint

(Aso et al., 2009)

adult lateral horn | faint

(Aso et al., 2009)

adult antennal nerve

(Aso et al., 2009)

adult deutocerebrum

(Aso et al., 2009)

adult tritocerebrum

(Aso et al., 2009)

adult gnathal ganglion

(Aso et al., 2009)

glial cell of adult brain

(Aso et al., 2009)

adult pars intercerebralis | faint

(Aso et al., 2009)

adult pars intercerebralis

(Connolly et al., 1996)

adult mushroom body alpha-lobe

(Pauls et al., 2010)

adult mushroom body beta-lobe

(Pauls et al., 2010)

adult mushroom body alpha'-lobe

(Pauls et al., 2010)

adult mushroom body beta'-lobe

(Pauls et al., 2010)

adult stage | male

adult mushroom body alpha-lobe

(Blum et al., 2009)

adult mushroom body beta-lobe

(Blum et al., 2009)

adult mushroom body gamma-lobe

(Blum et al., 2009)

Additional Information

Statement

Reference

Drives expression in the alpha, beta, alpha', beta' and gamma lobes of the mushroom body.

(FlyBase Curators, 2017)

Expression is limited to alpha, beta, and gamma neurons in the adult mushroom body.

(Blum et al., 2009)

Expression is found in only a few alpha'/beta' neurons.

(Krashes et al., 2007)

Expression of ScerGAL4^c309 is within a large subset of the Kenyon cells.

(Nitz et al., 2002)

Expression is highest in the mushroom bodies, but is generally widespread.

(Waddell et al., 2000)

In third instar larva, expression in antennal disc restricted to groups of cells.

(Manseau et al., 1997)

Expression in adult brain primarily in corpora pedunculata (mushroom body).

(Connolly et al., 1996)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Mmus\Prkaca^mC.UAS

(Joiner et al., 2006)

abnormal behavior | heat sensitive, with Mmus\Prkaca^mC.UAS

(Pitman et al., 2006)

abnormal behavior | heat sensitive, with shi^1.UAS

(Pitman et al., 2006, Kitamoto, 2002)

abnormal courtship behavior, with fru^RNAi.M.UAS

(Villella et al., 2005, Villella et al., 2005)

abnormal courtship behavior, with shi^1.UAS

(Broughton et al., 2004)

abnormal courtship behavior, with tra^UAS.cFa

(Villella et al., 2005)

abnormal courtship behavior | heat sensitive, with shi^1.UAS

(Villella et al., 2005, Villella et al., 2005, Kitamoto, 2002)

abnormal learning, with Gαs^Q215L.UAS

(Connolly et al., 1996)

abnormal learning | heat sensitive, with shi^1.UAS

(Dubnau et al., 2001)

abnormal locomotor behavior, with shi^1.UAS

(Broughton et al., 2004)

abnormal locomotor behavior | adult stage, with kis^GD16331

(Melicharek et al., 2010)

abnormal memory, with Nmdar1^N631Q.UAS

(Miyashita et al., 2012)

abnormal memory, with Pka-C1^UAS.Tag:FLAG

(Horiuchi et al., 2008)

abnormal memory | heat sensitive, with shi^1.UAS

(Dubnau et al., 2001)

abnormal neurophysiology | adult stage, with shi^1.UAS

(Nitz et al., 2002)

abnormal neurophysiology | heat sensitive, with shi^1.UAS

(Dubnau et al., 2001)

abnormal sleep, with Alk^GD42

(Bai and Sehgal, 2015)

abnormal sleep, with Alk^GD42, Scer\GAL80^Mef2.mb247

(Bai and Sehgal, 2015)

abnormal sleep, with Pka-R1^{BDK.UAS.Tag:HA}

(Crocker et al., 2010)

abnormal sleep | adult stage, with dco^GD1259

(Wang et al., 2022)

abnormal smell perception, with Gαq^RNAi.UAS

(Xia and Tully, 2007)

abnormal smell perception, with Gαs^Q215L.UAS

(Xia and Tully, 2007)

abnormal smell perception | heat sensitive, with shi^1.UAS

(Xia and Tully, 2007, McGuire et al., 2001)

abnormal song, with eag^DN.EKI.UAS

(Broughton et al., 2004)

abnormal song, with shi^1.UAS

(Villella et al., 2005, Broughton et al., 2004)

abnormal song | heat sensitive, with shi^1.UAS

(Villella et al., 2005, Villella et al., 2005)

abnormal visual behavior | heat sensitive, with shi^1.UAS

(van Swinderen et al., 2009)

chemical resistant, with slo^UAS.cGa

(Ghezzi et al., 2013)

decreased efficacy of medium-term memory | adult stage, with cac^JF02572

(Hidalgo et al., 2021)

decreased efficacy of medium-term memory | adult stage, with cac^KK101478

(Hidalgo et al., 2021)

decreased efficacy of short-term memory | adult stage, with cac^JF02572

(Hidalgo et al., 2021)

decreased efficacy of short-term memory | adult stage, with cac^KK101478

(Hidalgo et al., 2021)

decreased rate of movement, with shi^1.UAS

(Broughton et al., 2004)

increased sleep | adult stage, with dco^GD1259

(Wang et al., 2022)

increased sleep | adult stage, with Pka-C1^KK108966

(Machado Almeida et al., 2021)

partially lethal - majority die, with mys^RNAi.Sym.UAS

(Bhandari et al., 2006)

viable, with mys^RNAi.Sym.UAS

(Bhandari et al., 2006)

Phenotype Manifest In

adult brain, with shi^1.UAS

(Nitz et al., 2002)

adult head, with Optix^FL.UAS

(Weasner et al., 2007)

adult mushroom body, with shi^1.UAS

(Nitz et al., 2002, Dubnau et al., 2001)

antenna, with Optix^FL.UAS

(Weasner et al., 2007)

eye | ectopic, with Optix^FL.UAS

(Weasner et al., 2007)

eye | ectopic, with so::Optix^CT.UAS

(Weasner et al., 2007)

eye | ectopic, with so::Optix^NT.UAS

(Weasner et al., 2007)

eye | ectopic, with so::Optix^NT+CT.UAS

(Weasner et al., 2007)

seminal receptacle | ectopic, with tra^UAS.cFa

(Villella et al., 2005)

sex comb, with tra^UAS.cFa

(Villella et al., 2005)

spermatheca, with tra^UAS.cFa

(Villella et al., 2005)

spermatozoon, with tra^UAS.cFa

(Villella et al., 2005)

testis, with tra^UAS.cFa

(Villella et al., 2005)

Detailed Description

Statement

Reference

Inhibition of neural activity in Scer\GAL4^c309-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^c309, dco^GD1259 has decreased sleep | adult stage phenotype, suppressible by per⁰¹

(Wang et al., 2022)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^c309/chico^UAS.cNa rescues chico¹

(Naganos et al., 2012)

Scer\GAL4^c309/Dop1R1^UAS.cBa rescues Dop1R1^PL00420

(Bang et al., 2011)

Scer\GAL4^c309/Dop1R1^UAS.cBa rescues Df(3R)red-P52/+

(Bang et al., 2011)

Pde4^UAS.cCa/Scer\GAL4^c309 rescues Pde4^dnc-1

(van Swinderen et al., 2009)

Scer\GAL4^c309/Ptp10D^UAS.cQa rescues Ptp10D^chi

(Qian et al., 2007)

Partially rescues

Scer\GAL4^c309/Nf1^UAS.cdNa partially rescues Nf1^P2/Nf1^P1

(Bai et al., 2018)

Scer\GAL4^c309, inc^f00285 partially rescues inc^f00285

(Pfeiffenberger and Allada, 2012)

Scer\GAL4^c309, inc^f00285 partially rescues inc^f00285

(Stavropoulos and Young, 2011)

Scer\GAL4^c309/Adcy1^UAS.cZa partially rescues Adcy1^rut-2080

(Blum et al., 2009)

Scer\GAL4^c309/Pka-C1^UAS.cKa partially rescues Pka-C1^X4/Pka-C1^MB581

(Hong et al., 2008)

Adcy1^UAS.cHa/Scer\GAL4^c309 partially rescues Adcy1^rut-1

(Hong et al., 2008)

Fails to rescue

Scer\GAL4^c309/Dop2R^UAS.cDa fails to rescue Dop2R^attP

(Deng et al., 2019)

Tbh^UAS.cMa/Scer\GAL4^c309 fails to rescue Tbh^nM18

(Zhou et al., 2008)

amn^UAS.cWa/Scer\GAL4^c309 fails to rescue amn^X8

(Waddell et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

6906

w^*; P{GawB}c309

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

C309

(Kitamoto, 2002)

GAL4^C309

GAL4^c309

Scer\GAL4^c309

Name Synonyms

Secondary FlyBase IDs

FBal0058808

References (70)

Report Sections