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General Information
Symbol
Scer\GAL4ey-OK107
Species
S. cerevisiae
Name
FlyBase ID
FBal0058772
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
OK107-GAL4, OK107, GAL4-OK107, OK107GAL4, OK107 Gal4, eyOK107-gal4, P{GawB}eyOK107, ey-Gal4, 0K107-Gal4, OK107-G4
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    P{GawB}OK107
    Product class / Tool use(s)
    Expression Data
    Reporter Expression
    distribution deduced from reporter
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    eye

    Comment: faint expression

    ellipsoid body

    Comment: faint expression

    Additional Information
    Statement
    Reference
    Drives expression in the mushroom body and mushroom body neurons.
    Scer\GAL4ey-OK107 drives expression in neuroblasts, ganglion mother cells and neurons of the developing larval medulla.
    Scer\GAL4ey-OK107 drives expression in neurons that densely innervate the larval antennal lobes (AL). The cell bodies of labeled neurons are located in a cluster ventrolateral to the AL.
    The expression of ey, ap, and Dll were compared in outer optic lobes (OPC) starting in late third instar larvae. At this stage they were expressed as three distinct cell populations. In anterior sections, the three genes are expressed a three parallele stripes of cells that represent rows of neurons that emerge from the OPC. They correspond to progeny from the youngest to oldest neuroblasts. In middle sections, Dll-positive cells are generated in the progeny of the oldest neuroblasts, with ey-positive and ap-positive cells often placed below Dll-positive (in cells that had emerged earlier from the these neuroblasts). By the beginning of pupation, the number of cells origination from the OPC increased. A major reorganization of optic lobe structure occurs around P20 such that the three stripes are no longer distinguishable and the three cell populations are extensively interspersed within the adult medulla cortex. ey is expressed in neuroblasts and in the vast majority of neurons derived from ey-positive neuroblasts. Expression does not persist in pupae.
    Scer\GAL4ey-OK107 is expressed in the larval and adult mushroom body alpha'/beta', alpha/beta and gamma neurons and the Kenyon cells in the calyx. Co-expression with presynaptic markers labels restricted domains in the dendrites of Kenyon cells in the mushroom body lobes and calyx. A postsynaptic marker is expressed in the dendrites of Kenyon cells in the calyx, in a distinct domain to the pre-synapses.
    Expression of Scer\GAL4ey-OK107 is seen in cell bodies found in the local interneuron cluster located ventral to the antennal lobe. Most labelled cells are bilaterally projecting interneurons.
    Scer\GAL4ey-OK107 drives expression in the insulin producing cells (IPC) and in other cells of the dorsomedial neurosecretory cell cluster. Scer\GAL4ey-OK107 co-localises with Ilp2 protein in the IPC cells.
    Scer\GAL4ey-OK107 drives expression in GABAergic and glutamatergic antennal lobe local interneurons, and in mushroom body neurons.
    Expression is observed in the alpha, beta, gamma, alpha', and beta' neurons of the adult mushroom body.
    Scer\GAL4ey-OK107 is expressed in third instar larval lamina precursor cells.
    Scer\GAL4ey-OK107 drives expression in 30% of the cells in the medulla cortex, whose processes are preferentially located in the lower medulla layers. Cells labeled with Scer\GAL4ey-OK107 do not overlap with cells expressing Ecol\lacZap-rK568 or Scer\GAL4Dll-md23.
    Scer\GAL4ey-OK107 is expressed in all axons of the pedunculus.
     
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    gamma-lobe & neuron | somatic clone, with Fmr1UAS.cZa
    mushroom body & axon, with dlUY2278
    mushroom body & axon, with nosUY4741
    mushroom body & axon, with nosαTub84B.3'UTR.UAS
    mushroom body & axon, with Src64BUAS.cCa
    mushroom body & axon, with Src64BUY1332
    mushroom body & axon, with tumdsRNA.UAS.cBa
    mushroom body & axon, with tumdsRNA.UAS
    mushroom body & axon, with UY3164UY3164
    mushroom body & neuron & dendrite | somatic clone, with Fmr1UAS.cZa
    mushroom body & neuron | somatic clone, with Fmr1UAS.cZa
    Detailed Description
    Statement
    Reference
    Inhibition of neural activity in Scer\GAL4ey-OK107-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.
    Inhibition (using shits.UAS.cUa) of Scer\GAL4ey-OK107 neurons does not affect the reduction in food intake observed when larvae are moved to essential amino acid-deficient food, compared to controls.
    Activation of mushroom body neurons expressing Scer\GAL4Tab2-201Y through overexpression of temperature sensitive TrpA1 results in similar amounts of increased sleep in young (1 day) and mature (7-8 day) flies compared to controls.
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference
    NOT Enhanced by
    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference
    DAAMC.UASp, Scer\GAL4ey-OK107 has neuroanatomy defective phenotype, non-suppressible by dsh1/dsh[+]
    LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has neuroanatomy defective phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]
    Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Statement
    Reference
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference
    LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, enhanceable by Rac2Δ/Rac1J6/Rac2[+]/Rac1[+]
    NOT Enhanced by
    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference
    DAAMC.UASp, Scer\GAL4ey-OK107 has mushroom body phenotype, non-suppressible by dsh1/dsh[+]
    LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]
    Enhancer of
    Statement
    Reference
    NOT Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference
    Scer\GAL4ey-OK107/tsrS3A.UAS is a suppressor of adult mushroom body & axon | somatic clone phenotype of ssh1-11
    Scer\GAL4ey-OK107/tsrUAS.cNa is a suppressor of adult mushroom body & axon | somatic clone phenotype of ssh1-11
    Scer\GAL4ey-OK107/tsrS3A.UAS is a suppressor of antennal lobe & axon | somatic clone phenotype of ssh1-11
    Scer\GAL4ey-OK107/tsrUAS.cNa is a suppressor of antennal lobe & axon | somatic clone phenotype of ssh1-11
    Scer\GAL4ey-OK107/tsrS3A.UAS is a suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh1-11
    Scer\GAL4ey-OK107/tsrUAS.cNa is a suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh1-11
    NOT Suppressor of
    Statement
    Reference
    tsrS3E.UAS/Scer\GAL4ey-OK107 is a non-suppressor of adult mushroom body & axon | somatic clone phenotype of ssh1-11
    tsrS3E.UAS/Scer\GAL4ey-OK107 is a non-suppressor of antennal lobe & axon | somatic clone phenotype of ssh1-11
    tsrS3E.UAS/Scer\GAL4ey-OK107 is a non-suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh1-11
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Rescues
    Partially rescues
    Fails to rescue
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    Scer\GAL4OK107
    Scer\GAL4ey-OK107
    Name Synonyms
    Secondary FlyBase IDs
      References (324)