Open Close
General Information
Symbol
Scer\GAL4ey-OK107
Species
S. cerevisiae
Name
FlyBase ID
FBal0058772
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
OK107-GAL4, OK107, GAL4-OK107, eyOK107-gal4, OK107GAL4, OK107 Gal4, P{GawB}eyOK107, ey-Gal4, 0K107-Gal4, OK107-G4
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    transposable element insertion site
    (Akiba et al., 2020, Amin et al., 2020, Baral et al., 2020, Caipo et al., 2020, Lai et al., 2020, Morton et al., 2020, Sabandal et al., 2020, Samuels et al., 2020, Serway et al., 2020, Sudarsanam et al., 2020, Tamberg et al., 2020, Vissers et al., 2020, Yaniv et al., 2020, Yuan et al., 2020, Zhang et al., 2020, Bhukel et al., 2019, Bielopolski et al., 2019, Chen et al., 2019, Goyal et al., 2019, Guo et al., 2019, Handler et al., 2019, Higham et al., 2019, Higham et al., 2019, Jia et al., 2019, Kang et al., 2019, Lacin et al., 2019, Li and Sean Millard, 2019, Marmor-Kollet et al., 2019, Pahl et al., 2019, Pírez et al., 2019, Sapiro et al., 2019, Schoborg et al., 2019, Sears et al., 2019, Sengupta et al., 2019, Shyu et al., 2019, Song et al., 2019, Sullivan et al., 2019, Takayanagi-Kiya and Kiya, 2019, Zhao et al., 2019, Contreras et al., 2018, Fattahi et al., 2018, Flybrain Neuron Database et al., 2018.6.28, Goda et al., 2018, Gouzi et al., 2018, Groschner et al., 2018, Huang et al., 2018, Lee et al., 2018, Loewen et al., 2018, Lüdke et al., 2018, Martín-Peña et al., 2018, Panikker et al., 2018, Ran et al., 2018, Sanchez-Garcia and Fernandez-Funez, 2018, Sonn et al., 2018, Tran et al., 2018, Tsai et al., 2018, Widmer et al., 2018, Akbergenova and Littleton, 2017, Aranha et al., 2017, Bienkowski et al., 2017, Boppana et al., 2017, Li et al., 2017, Marchetti and Tavosanis, 2017, Martin-Peña et al., 2017, Mohammad et al., 2017, Murakami et al., 2017, Rohith and Shyamala, 2017, Yang et al., 2017, Zwarts et al., 2017, Blumröder et al., 2016, Bouché et al., 2016, Dollar et al., 2016, Dong et al., 2016, Drago and Davis, 2016, Fernandez-Funez et al., 2016, Furukubo-Tokunaga et al., 2016, Gai et al., 2016, Guven-Ozkan et al., 2016, Kelly et al., 2016, Kraft et al., 2016, Lai et al., 2016, Liu et al., 2016, Naganos et al., 2016, Ng et al., 2016, Oliva et al., 2016, Rabinovich et al., 2016, Rohwedder et al., 2016, Sanchez-Garcia et al., 2016, Schwartz et al., 2016, Xu et al., 2016, Yagi et al., 2016, Yang et al., 2016, Yuan et al., 2016, Zwarts et al., 2016, Afonso et al., 2015, Bai and Sehgal, 2015, Bornstein et al., 2015, Bruckert et al., 2015, Chakraborty et al., 2015, Chen et al., 2015, Chen et al., 2015, Cohn et al., 2015, Fernandez-Funez et al., 2015, Gombos et al., 2015, Kirkhart and Scott, 2015, Okamoto and Nishimura, 2015, Okumura et al., 2015, Sakuma et al., 2015, Shankar et al., 2015, Shih et al., 2015, Siegenthaler et al., 2015, Silva et al., 2015, Tabuchi et al., 2015, Zhang et al., 2015, Abe et al., 2014, Bertet et al., 2014, Bjordal et al., 2014, Cavanaugh et al., 2014, Choksi et al., 2014, DasGupta et al., 2014, Fropf et al., 2014, Gatto et al., 2014, Homem et al., 2014, Kayser et al., 2014, Lin et al., 2014, Luhur et al., 2014, Marchetti et al., 2014, Rusan et al., 2014, Xu et al., 2014, Baggio et al., 2013, Bräcker et al., 2013, Cavaliere et al., 2013, Devineni et al., 2013, Fitzsimons et al., 2013, Fujita et al., 2013, Ghezzi et al., 2013, Gross et al., 2013, Gupta et al., 2013, Levy and Larsen, 2013, Li et al., 2013, Li et al., 2013, Lin et al., 2013, Malik et al., 2013, Manansala et al., 2013, Melzer et al., 2013, Rahn et al., 2013, Rallis et al., 2013, Sanchez-Garcia et al., 2013, Suzuki et al., 2013, Ueno et al., 2013, Weislogel et al., 2013, Wong et al., 2013, Wu et al., 2013, Yu et al., 2013, Beuchle et al., 2012, Butcher et al., 2012, Chen and Chiang, 2012.5.22, Chen et al., 2012, Chen et al., 2012, Coffee et al., 2012, Eddison et al., 2012, Henry et al., 2012, Ishida et al., 2012, Jepson et al., 2012, Kunz et al., 2012, Liu et al., 2012, Luo et al., 2012, Lv et al., 2012, Miyashita et al., 2012, Naganos et al., 2012, Plavicki et al., 2012, Sakai et al., 2012, Scheunemann et al., 2012, Tanenhaus et al., 2012, Volders et al., 2012, Wu et al., 2012, Yaniv et al., 2012, Brooks et al., 2011, Bushey et al., 2011, Casas-Tinto et al., 2011, Chen et al., 2011, Chen et al., 2011, Christiansen et al., 2011, Copf et al., 2011, Das et al., 2011, Fitzsimons and Scott, 2011, Goossens et al., 2011, Gouzi et al., 2011, Hampel et al., 2011, Henthorn et al., 2011, Honegger et al., 2011, Huang et al., 2011, Jepson et al., 2011, Kang et al., 2011, Kaun et al., 2011, King et al., 2011, Knapek et al., 2011, Lee et al., 2011, Lin et al., 2011, Mochizuki et al., 2011, Morante et al., 2011, Neely et al., 2011, Pandey et al., 2011, Shimizu et al., 2011, Shin and Diantonio, 2011, Stavropoulos and Young, 2011, Sweeney et al., 2011, Tessier and Broadie, 2011, Thum et al., 2011, Wang et al., 2011, Wang et al., 2011, Bates et al., 2010, Benna et al., 2010, Besson et al., 2010, Chou et al., 2010, Coffee et al., 2010, Enell et al., 2010, Fernandez-Funez et al., 2010, Hekmat-Scafe et al., 2010, Kosmidis et al., 2010, Kremer et al., 2010, Li et al., 2010, Luheshi et al., 2010, Melicharek et al., 2010, Owald et al., 2010, Papanikolopoulou et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Rallis et al., 2010, Shuai et al., 2010, Tamura et al., 2010, Wang et al., 2010, Wu et al., 2010, Aso et al., 2009, Blum et al., 2009, Clements et al., 2009, Donlea et al., 2009, Evans et al., 2009, Evans et al., 2009, Fernandez-Funez et al., 2009, Furukubo-Tokunaga et al., 2009, Higuchi et al., 2009, Iijima-Ando et al., 2009, Kim et al., 2009, Krashes et al., 2009, Kurusu et al., 2009, Lee et al., 2009, Leiss et al., 2009, Lin et al., 2009, Lin et al., 2009, Mao and Davis, 2009, Miller et al., 2009, Miller et al., 2009, Pagani et al., 2009, Sung et al., 2009, Yu et al., 2009, Yu et al., 2009, Bolduc et al., 2008, Branco et al., 2008, Chen et al., 2008, Clements et al., 2008, Hong et al., 2008, Hoopfer et al., 2008, Iijima-Ando et al., 2008, Iijima et al., 2008, Johard et al., 2008, Kurusu and Zinn, 2008, Morante and Desplan, 2008, Nässel et al., 2008, Nishimura et al., 2008, Pan et al., 2008, Phillips et al., 2008, Plaza et al., 2008, Prince et al., 2008, Sawamura et al., 2008, Schuldiner et al., 2008, Tanaka et al., 2008, Tessier and Broadie, 2008, Wu et al., 2008, Wu et al., 2008, Yang et al., 2008, Al-Ramahi et al., 2007, Campusano et al., 2007, Funada et al., 2007, Fushima and Tsujimura, 2007, Komiyama et al., 2007, Lin et al., 2007, Lu et al., 2007, Martin et al., 2007, Qian et al., 2007, Rolls et al., 2007, Shi et al., 2007, Tzortzopoulos and Skoulakis, 2007, Whited et al., 2007, Wu et al., 2007, Yamazaki et al., 2007, Zhang et al., 2007, Boyle et al., 2006, Gu and O'Dowd, 2006, Kobayashi et al., 2006, Lai and Lee, 2006, Sakai and Kitamoto, 2006, Wang et al., 2006, Zheng et al., 2006, Zhu et al., 2006, Agrawal et al., 2005, Goldstein et al., 2005, Hoehne et al., 2005, Jiang et al., 2005, Manoli et al., 2005, Martini and Davis, 2005, Masuda-Nakagawa et al., 2005, Orihara-Ono et al., 2005, Pascual et al., 2005, Ramaekers et al., 2005, Yang et al., 2005, Zhu et al., 2005, Awasaki and Ito, 2004, Gu et al., 2004, Ng and Luo, 2004, Pan et al., 2004, Suh et al., 2004, Wang et al., 2004, Wang et al., 2004, Wojtowicz et al., 2004, Zelhof and Hardy, 2004, Zhan et al., 2004, Adachi et al., 2003, Kim et al., 2003, Nicolai et al., 2003, Reuter et al., 2003, Su and O'Dowd, 2003, Watts et al., 2003, Zheng et al., 2003, Zhu et al., 2003, Clements and Callaerts, 2002, Kitamoto, 2002, Kurusu et al., 2002, Ng et al., 2002, Niimi et al., 2002, Wang et al., 2002, Billuart et al., 2001, McGuire et al., 2001, Pascual and Preat, 2001, Plaza et al., 2001, Scott et al., 2001, DeZazzo et al., 2000, Liu et al., 2000, Martini et al., 2000, Lee et al., 1999, Connolly et al., 1996)
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    P{GawB}OK107
    Product class / Tool use(s)
    Expression Data
    Reporter Expression
    distribution deduced from reporter
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    eye

    Comment: faint expression

    ellipsoid body

    Comment: faint expression

    secondary neuroblast

    Comment: only expressed in 'old' secondary neuroblasts.

    Additional Information
    Statement
    Reference

    Drives expression in the mushroom body and mushroom body neurons.

    Scer\GAL4ey-OK107 drives expression in neuroblasts, ganglion mother cells and neurons of the developing larval medulla.

    Scer\GAL4ey-OK107 drives expression in neurons that densely innervate the larval antennal lobes (AL). The cell bodies of labeled neurons are located in a cluster ventrolateral to the AL.

    The expression of ey, ap, and Dll were compared in outer optic lobes (OPC) starting in late third instar larvae. At this stage they were expressed as three distinct cell populations. In anterior sections, the three genes are expressed a three parallele stripes of cells that represent rows of neurons that emerge from the OPC. They correspond to progeny from the youngest to oldest neuroblasts. In middle sections, Dll-positive cells are generated in the progeny of the oldest neuroblasts, with ey-positive and ap-positive cells often placed below Dll-positive (in cells that had emerged earlier from the these neuroblasts). By the beginning of pupation, the number of cells origination from the OPC increased. A major reorganization of optic lobe structure occurs around P20 such that the three stripes are no longer distinguishable and the three cell populations are extensively interspersed within the adult medulla cortex. ey is expressed in neuroblasts and in the vast majority of neurons derived from ey-positive neuroblasts. Expression does not persist in pupae.

    Scer\GAL4ey-OK107 is expressed in the larval and adult mushroom body alpha'/beta', alpha/beta and gamma neurons and the Kenyon cells in the calyx. Co-expression with presynaptic markers labels restricted domains in the dendrites of Kenyon cells in the mushroom body lobes and calyx. A postsynaptic marker is expressed in the dendrites of Kenyon cells in the calyx, in a distinct domain to the pre-synapses.

    Expression of Scer\GAL4ey-OK107 is seen in cell bodies found in the local interneuron cluster located ventral to the antennal lobe. Most labelled cells are bilaterally projecting interneurons.

    Scer\GAL4ey-OK107 drives expression in the insulin producing cells (IPC) and in other cells of the dorsomedial neurosecretory cell cluster. Scer\GAL4ey-OK107 co-localises with Ilp2 protein in the IPC cells.

    Scer\GAL4ey-OK107 drives expression in GABAergic and glutamatergic antennal lobe local interneurons, and in mushroom body neurons.

    Expression is observed in the alpha, beta, gamma, alpha', and beta' neurons of the adult mushroom body.

    Scer\GAL4ey-OK107 is expressed in third instar larval lamina precursor cells.

    Scer\GAL4ey-OK107 drives expression in 30% of the cells in the medulla cortex, whose processes are preferentially located in the lower medulla layers. Cells labeled with Scer\GAL4ey-OK107 do not overlap with cells expressing Ecol\lacZap-rK568 or Scer\GAL4Dll-md23.

    Scer\GAL4ey-OK107 is expressed in all axons of the pedunculus.

     
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In

    gamma-lobe & neuron | somatic clone, with Fmr1UAS.cZa

    mushroom body & axon, with dlUY2278

    mushroom body & axon, with nosUY4741

    mushroom body & axon, with nosαTub84B.3'UTR.UAS

    mushroom body & axon, with Src64BUAS.cCa

    mushroom body & axon, with Src64BUY1332

    mushroom body & axon, with tumdsRNA.UAS.cBa

    mushroom body & axon, with tumdsRNA.UAS

    mushroom body & axon, with UY3164UY3164

    mushroom body & neuron & dendrite | somatic clone, with Fmr1UAS.cZa

    mushroom body & neuron | somatic clone, with Fmr1UAS.cZa

    Detailed Description
    Statement
    Reference

    Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4ey-OK107 cells in adults during testing significantly reduces immediate or 24 h aversive olfactory memory, compared to controls; there is no effect on the increase in aversive olfactory 24 h memory observed when the recall environment better matches the training environment (presence of copper grid), compared to controls.

    Inhibition of neural activity in Scer\GAL4ey-OK107-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

    Inhibition (using shits.UAS.cUa) of Scer\GAL4ey-OK107 neurons does not affect the reduction in food intake observed when larvae are moved to essential amino acid-deficient food, compared to controls.

    Activation of mushroom body neurons expressing Scer\GAL4Tab2-201Y through overexpression of temperature sensitive TrpA1 results in similar amounts of increased sleep in young (1 day) and mature (7-8 day) flies compared to controls.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference
    NOT Enhanced by
    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference

    DAAMC.UASp, Scer\GAL4ey-OK107 has neuroanatomy defective phenotype, non-suppressible by dsh1/dsh[+]

    LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has neuroanatomy defective phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]

    Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Statement
    Reference
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, enhanceable by Rac2Δ/Rac1J6/Rac2[+]/Rac1[+]

    NOT Enhanced by
    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference

    DAAMC.UASp, Scer\GAL4ey-OK107 has mushroom body phenotype, non-suppressible by dsh1/dsh[+]

    LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]

    Enhancer of
    Statement
    Reference
    NOT Enhancer of
    Suppressor of
    Statement
    Reference

    Scer\GAL4ey-OK107/tsrS3A.UAS is a suppressor of adult mushroom body & axon | somatic clone phenotype of ssh1-11

    Scer\GAL4ey-OK107/tsrUAS.cNa is a suppressor of adult mushroom body & axon | somatic clone phenotype of ssh1-11

    Scer\GAL4ey-OK107/tsrS3A.UAS is a suppressor of antennal lobe & axon | somatic clone phenotype of ssh1-11

    Scer\GAL4ey-OK107/tsrUAS.cNa is a suppressor of antennal lobe & axon | somatic clone phenotype of ssh1-11

    Scer\GAL4ey-OK107/tsrS3A.UAS is a suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh1-11

    Scer\GAL4ey-OK107/tsrUAS.cNa is a suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh1-11

    NOT Suppressor of
    Statement
    Reference

    tsrS3E.UAS/Scer\GAL4ey-OK107 is a non-suppressor of adult mushroom body & axon | somatic clone phenotype of ssh1-11

    tsrS3E.UAS/Scer\GAL4ey-OK107 is a non-suppressor of antennal lobe & axon | somatic clone phenotype of ssh1-11

    tsrS3E.UAS/Scer\GAL4ey-OK107 is a non-suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh1-11

    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Rescues
    Partially rescues
    Fails to rescue
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (6)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    Scer\GAL4OK107
    Scer\GAL4ey-OK107
    Name Synonyms
    Secondary FlyBase IDs
      References (357)