FB2025_01 , released February 20, 2025
Allele: Scer\GAL4Act5C.PP
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General Information
Symbol
Scer\GAL4Act5C.PP
Species
S. cerevisiae
Name
Actin 5C promoter construct of Pignoni
FlyBase ID
FBal0058815
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Act-GAL4, Act>cd2>GAL4, act5C-GAL4, Act5c>CD2>Gal4, actin-Gal4, ActinGAL4, Act>Gal4, act > CD2 > Gal4, act>stop>Gal4, Act5C>GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
(Simcox et al., 2008, Li and Baker, 2007, Murakami et al., 2007, Bras-Pereira et al., 2006, Callejo et al., 2006, Miura et al., 2006, Schneider et al., 2006, Becam et al., 2005, Collins and Cohen, 2005, Ho et al., 2005, Sustar and Schubiger, 2005, Ward and Berg, 2005, Wittwer et al., 2005, Bateman and McNeill, 2004, Huh et al., 2004, Pierce et al., 2004, Shcherbata et al., 2004, Torroja et al., 2004, Wang and Jiang, 2004, Zirin and Mann, 2004, Besse and Pret, 2003, Deng et al., 2003, Emerald et al., 2003, Johnston and Sanders, 2003, Raisin et al., 2003, Szuplewski et al., 2003, Ahmad and Baker, 2002, Bai and Montell, 2002, Bessa et al., 2002, Besse et al., 2002, Britton et al., 2002, Chern and Choi, 2002, Chu et al., 2002, Duman-Scheel et al., 2002, Galant et al., 2002, Hennig and Neufeld, 2002, Hipfner et al., 2002, Jia et al., 2002, Kopp and Duncan, 2002, Peri et al., 2002, Prober and Edgar, 2002, Tseng and Hariharan, 2002, Wu and Cohen, 2002, Xin et al., 2002, Amanai and Jiang, 2001, Dobens et al., 2001, Halfar et al., 2001, Keisman and Baker, 2001, Lee and Treisman, 2001, Lee et al., 2001, Milan et al., 2001, Miron et al., 2001, Tapon et al., 2001, Tulina and Matunis, 2001, Weihe et al., 2001, Casares and Mann, 2000, Certel et al., 2000, Dahmann and Basler, 2000, Denef et al., 2000, Dobens et al., 2000, Dong et al., 2000, Entchev et al., 2000, Milan and Cohen, 2000, Prober and Edgar, 2000, Rulifson et al., 2000, Wang et al., 2000, Burke et al., 1999, Jarecki et al., 1999, Larkin et al., 1999, Luo et al., 1999, Methot and Basler, 1999, Rangarajan et al., 1999, Verdu et al., 1999, Wu and Cohen, 1999, Abu-Shaar and Mann, 1998, Britton and Edgar, 1998, Cadigan et al., 1998, Halder et al., 1998, Hazelett et al., 1998, Kozopas et al., 1998, Neufeld et al., 1998, Pai et al., 1998, Gorfinkiel et al., 1997, Pignoni and Zipursky, 1997)
Progenitor genotype
(Simcox et al., 2008, Li and Baker, 2007, Murakami et al., 2007, Bras-Pereira et al., 2006, Callejo et al., 2006, Miura et al., 2006, Schneider et al., 2006, Becam et al., 2005, Collins and Cohen, 2005, Frei et al., 2005, Ho et al., 2005, Rogers et al., 2005, Sustar and Schubiger, 2005, Ward and Berg, 2005, Wittwer et al., 2005, Bateman and McNeill, 2004, Chang et al., 2004, de la Cova et al., 2004, Frei and Edgar, 2004, Huh et al., 2004, Pierce et al., 2004, Shcherbata et al., 2004, Torroja et al., 2004, Wang and Jiang, 2004, Zirin and Mann, 2004, Besse and Pret, 2003, Deng et al., 2003, Emerald et al., 2003, Estella et al., 2003, Johnston and Sanders, 2003, Milan and Cohen, 2003, Perrin et al., 2003, Raisin et al., 2003, Szuplewski et al., 2003, Ahmad and Baker, 2002, Bai and Montell, 2002, Bessa et al., 2002, Besse et al., 2002, Britton et al., 2002, Chern and Choi, 2002, Chu et al., 2002, Duman-Scheel et al., 2002, Galant et al., 2002, Giráldez et al., 2002, Hennig and Neufeld, 2002, Hipfner et al., 2002, Jia et al., 2002, Kopp and Duncan, 2002, Martin-Castellanos and Edgar, 2002, Peri et al., 2002, Prober and Edgar, 2002, Stevaux et al., 2002, Tseng and Hariharan, 2002, Wu and Cohen, 2002, Xin et al., 2002, Amanai and Jiang, 2001, Dobens et al., 2001, Halfar et al., 2001, Keisman and Baker, 2001, Lee and Treisman, 2001, Lee et al., 2001, Miron et al., 2001, Tapon et al., 2001, Tulina and Matunis, 2001, Weihe et al., 2001, Casares and Mann, 2000, Certel et al., 2000, Dahmann and Basler, 2000, Denef et al., 2000, Dobens et al., 2000, Dong et al., 2000, Entchev et al., 2000, Milan and Cohen, 2000, Prober and Edgar, 2000, Rulifson et al., 2000, Wang et al., 2000, Zhang et al., 2000, Burke et al., 1999, Erkner et al., 1999, Jarecki et al., 1999, Larkin et al., 1999, Luo et al., 1999, Methot and Basler, 1999, Rangarajan et al., 1999, Verdu et al., 1999, Wu and Cohen, 1999, Abu-Shaar and Mann, 1998, Britton and Edgar, 1998, Cadigan et al., 1998, Halder et al., 1998, Hazelett et al., 1998, Kozopas et al., 1998, Neufeld et al., 1998, Pai et al., 1998, Gorfinkiel et al., 1997, Pignoni and Zipursky, 1997)
Carried in construct
Cytology
Description

FRT cassette has been excised, juxtaposing Act5C promoter with GAL4 driver coding sequences.

Allele components
Component
Use(s)
Regulatory region(s)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Designed for use in mosaic analyses; used to drive gene of interest in ScerFLP1-generated clones. Transiently generated during mosaic analyses; ubiquitous expression throughout region of the generated clone, but not in rest of body (cell autonomous). Stocks are attached to progenitor construct P{GAL4-Act5C(FRT.CD2).P}; see ScerGAL4FRT.RnorCd2.Act5C elsewhere in this table.

Transiently generated during mosaic analyses; ubiquitous expression throughout region of the generated clone, but not in rest of body (cell autonomous).

Designed for use in mosaic analyses; used to drive gene of interest in ScerFLP1-generated clones.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61αNIG.9539R

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61αUAS.cKa.Tag:FLAG

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61βGD3787

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Sec61γGD4660

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp14GD13498

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp54kGD1542

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Srp68GD11522

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with SrpRβGD2371

autophagic vacuole & larval fat body | somatic clone | cell autonomous, with Xbp1UAS.RB

cell surface & nurse cell | somatic clone, with Pi3K92EUAS.Tag:MYC

eye disc & photoreceptor cell, with dppUAS.cFa

eye disc & photoreceptor cell, with Ras85DV12.UAS

eye disc & subretinal glial cell, with dppUAS.cFa

follicle cell & nucleus | somatic clone, with CycEUAS.cLa

follicle cell & nucleus | somatic clone, with dapUAS.cdNa

follicle cell & nucleus | somatic clone, with fzrUAS.cSa

follicle cell & nucleus | somatic clone, with hidUAS.cCa

follicle cell & nucleus | somatic clone, with MycUAS.cZa

follicle cell & nucleus | somatic clone, with rprUAS.cCa

glial cell & eye | ectopic, with dppUAS.cSa

glial cell & eye disc | somatic clone, with ciZn:C.UAS

glial cell & eye disc | somatic clone | cell non-autonomous, with hhUAS.cAa

imaginal disc & cell, with Pi3K21BUAS.Tag:HA

imaginal disc & cell, with Pi3K92EUAS.Tag:MYC

leg & cuticle | dorsal | somatic clone, with tshUAS.cGa

leg & cuticle | somatic clone, with tshUAS.cGa

leg & cuticle | ventral | somatic clone, with tshUAS.cGa

lysosome & larval fat body | somatic clone | cell autonomous, with LeuRS-mNIG.7479R

lysosome & larval fat body | somatic clone | cell autonomous, with MetRS-mNIG.8684R

lysosome & larval fat body | somatic clone | cell autonomous, with PheRS-mNIG.13348R

lysosome & larval fat body | somatic clone | cell autonomous, with ProRS-mNIG.12186R

lysosome & larval fat body | somatic clone | cell autonomous, with RFeSPNIG.7361R

lysosome & larval fat body | somatic clone | cell autonomous, with Sec61αNIG.9539R

lysosome & larval fat body | somatic clone | cell autonomous, with Sec61αUAS.cKa.Tag:FLAG

lysosome & larval fat body | somatic clone | cell autonomous, with Sec61βGD3787

lysosome & larval fat body | somatic clone | cell autonomous, with Sec61γGD4660

lysosome & larval fat body | somatic clone | cell autonomous, with Srp14GD13498

lysosome & larval fat body | somatic clone | cell autonomous, with Srp54kGD1542

lysosome & larval fat body | somatic clone | cell autonomous, with Srp68GD11522

lysosome & larval fat body | somatic clone | cell autonomous, with SrpRβGD2371

lysosome & larval fat body | somatic clone | cell autonomous, with TyrRSNIG.4561R

lysosome & larval fat body | somatic clone | cell autonomous, with Xbp1UAS.RB

nurse cell & cytoskeleton | somatic clone, with rprUAS.cCa

nurse cell & nucleus | somatic clone, with rprUAS.cCa

photoreceptor cell & axon, with NetAUAS.cHa

photoreceptor cell & axon, with NetBUAS.cHa

Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference

bskDN.UAS/Scer\GAL4Act5C.PP is a suppressor of increased cell death | somatic clone | nutrition conditional phenotype of Pten117

bskDN.UAS/Scer\GAL4Act5C.PP is a suppressor of increased cell growth rate | somatic clone | nutrition conditional phenotype of Pten117

NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4Act5C.PP, sqaRNAi.UAS has fat body | somatic clone | calorie restriction conditional phenotype, suppressible by sqhDD.UAS/Scer\GAL4Act5C.PP

Scer\GAL4Act5C.PP, sqaRNAi.UAS has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible by sqhDD.UAS/Scer\GAL4Act5C.PP

Scer\GAL4Act5C.PP, sqaT279A.UAS has fat body | somatic clone | calorie restriction conditional phenotype, suppressible | partially by sqhT20E.S21E.UAS/Scer\GAL4Act5C.PP

Scer\GAL4Act5C.PP, sqaT279A.UAS has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible | partially by sqhT20E.S21E.UAS/Scer\GAL4Act5C.PP

Atg12RNAi.UAS, Scer\GAL4Act5C.PP has fat body | somatic clone | calorie restriction conditional phenotype, suppressible by sqhT20A.S21A.UAS, Scer\GAL4Act5C.PP

Atg12RNAi.UAS, Scer\GAL4Act5C.PP has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible by sqhT20A.S21A.UAS, Scer\GAL4Act5C.PP

Atg12RNAi.UAS, Scer\GAL4Act5C.PP has fat body | somatic clone | calorie restriction conditional phenotype, suppressible by sqaT279A.UAS, Scer\GAL4Act5C.PP

Atg12RNAi.UAS, Scer\GAL4Act5C.PP has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible by sqaT279A.UAS, Scer\GAL4Act5C.PP

Atg12RNAi.UAS, Scer\GAL4Act5C.PP has fat body | somatic clone | calorie restriction conditional phenotype, suppressible by sqaRNAi.UAS, Scer\GAL4Act5C.PP

Atg12RNAi.UAS, Scer\GAL4Act5C.PP has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible by sqaRNAi.UAS, Scer\GAL4Act5C.PP

NOT suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference

bskDN.UAS/Scer\GAL4Act5C.PP is a suppressor of eye | somatic clone | nutrition conditional phenotype of Pten117

sqhDD.UAS/Scer\GAL4Act5C.PP is a suppressor of fat body | somatic clone | calorie restriction conditional phenotype of Scer\GAL4Act5C.PP, sqaRNAi.UAS

sqhDD.UAS/Scer\GAL4Act5C.PP is a suppressor of autophagosome | somatic clone | calorie restriction conditional phenotype of Scer\GAL4Act5C.PP, sqaRNAi.UAS

sqhT20E.S21E.UAS/Scer\GAL4Act5C.PP is a suppressor | partially of fat body | somatic clone | calorie restriction conditional phenotype of Scer\GAL4Act5C.PP, sqaT279A.UAS

sqhT20E.S21E.UAS/Scer\GAL4Act5C.PP is a suppressor | partially of autophagosome | somatic clone | calorie restriction conditional phenotype of Scer\GAL4Act5C.PP, sqaT279A.UAS

sqhT20A.S21A.UAS, Scer\GAL4Act5C.PP is a suppressor of fat body | somatic clone | calorie restriction conditional phenotype of Atg12RNAi.UAS, Scer\GAL4Act5C.PP

sqhT20A.S21A.UAS, Scer\GAL4Act5C.PP is a suppressor of autophagosome | somatic clone | calorie restriction conditional phenotype of Atg12RNAi.UAS, Scer\GAL4Act5C.PP

sqaT279A.UAS, Scer\GAL4Act5C.PP is a suppressor of fat body | somatic clone | calorie restriction conditional phenotype of Atg12RNAi.UAS, Scer\GAL4Act5C.PP

sqaT279A.UAS, Scer\GAL4Act5C.PP is a suppressor of autophagosome | somatic clone | calorie restriction conditional phenotype of Atg12RNAi.UAS, Scer\GAL4Act5C.PP

sqaRNAi.UAS, Scer\GAL4Act5C.PP is a suppressor of fat body | somatic clone | calorie restriction conditional phenotype of Atg12RNAi.UAS, Scer\GAL4Act5C.PP

sqaRNAi.UAS, Scer\GAL4Act5C.PP is a suppressor of autophagosome | somatic clone | calorie restriction conditional phenotype of Atg12RNAi.UAS, Scer\GAL4Act5C.PP

NOT Suppressor of
Statement
Reference

hhUAS.cPb/Scer\GAL4Act5C.PP is a non-suppressor of phenotype of enApa

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Exists in clones, not reported as being germ-line transmitted.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
GAL4Act5C.PP
Scer\GAL4Act5C.PP
Name Synonyms
Actin 5C promoter construct of Pignoni
Secondary FlyBase IDs
    References (393)