|Name||Saccharomyces cerevisiae UAS construct a of Connolly||FlyBase ID||FBal0058938|
|Feature type||allele||Associated gene||Dmel\Gαs|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Expression of G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P2.4.Pdf] significantly reduces the response of the M pacemaker neurons in the brain to Pdf and to Dh31. Expression of G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[Mai179] significantly reduces the response of the E pacemaker neurons in the brain to Pdf.
Over 60% of flies expressing G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P0.5.Pdf] in constant darkness are arrhythmic. While the remaining rhythmic flies exhibit normal periods, they are only weakly rhythmic, as shown by a lower rhythm power than control flies.
Expression of G-salpha60A[Scer\UAS.cCa] under the control of either Scer\GAL4[c309], Scer\GAL4 (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4[Tab2-201Y] (in the γ-lobes) has no effect on spontaneous odor intensity or identity discrimination, nor conditioned odor intensity and identity discrimination.
Expression of G-sα60AScer\UAS.cCa under the control of Scer\GAL4c205 has no effect on visual pattern memory.
When expression of G-sα60AScer\UAS.cCa is driven in the dopamine and serotonin neurons by Scer\GAL4Ddc.PL, flies display a reduced responsiveness to an initial dose of cocaine compared to wild-type. Also no sensitization was seen on repeated exposures to cocaine, in contrast to wild-type. Flies expressing G-iα65AScer\UAS.cLa under the control of Scer\GAL4Ddc.PL show significantly decreased locomotor responses to the dopamine D2-like agonist quinpirole compared to controls. Flies expressing G-iα65AScer\UAS.cLa under the control of Scer\GAL4Ddc.PL exhibit no overt behavioural or lethality phenotypes are seen.
When expression is driven by Scer\GAL4238Y, Scer\GAL4c309, Scer\GAL4c747 or Scer\GAL4Tab2-201Y no effect on learning is seen.
|Phenotype Manifest In|
Co-expression of Pdfr[Scer\UAS.cMa] fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P2.4.Pdf]. Co-expression of Dh31-R1[Scer\UAS.cDa] fully rescues the response of the M pacemaker neurons in the brain to Dh31 to wild-type levels in flies expressing G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P2.4.Pdf].
Co-expression of Gr21a[Scer\UAS.cKa] and Gr63a[Scer\UAS.cKa] under the control of Scer\GAL4[Or22a.8010] in the "empty neuron" system ("Δhalo" embryos - Dp(2;2)dpp[d21] + Df(2L)dp-79b - in which Or22a and Or22b are deleted) confers a modest response to CO[] in the ab3A olfactory receptor neuron (ORN). Additional co-expression of G-sα60A[Scer\UAS.cCa] does not increase the response of the ab3A ORN to CO[].
Animals co-expressing G-sα60A[Scer\UAS.cCa] and AdoR[Scer\UAS.cDa] under the control of Scer\GAL4[en-e16E] show 20% lethality as pharate adults, whereas expression of either G-sα60A[Scer\UAS.cCa] or AdoR[Scer\UAS.cDa] alone under the control of Scer\GAL4[en-e16E] does not cause lethality. The flies that do emerge show strong wing deformation and melanisation.
|Complementation & Rescue Data|
Expression of G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[Orco.T:Hsim\VP16] in homozygous G-sα60A[R60] ab3A olfactory receptor neurons rescues the reduced odour response seen in these neurons at near saturating concentrations of either methyl butyrate or butyric acid.
G-sα60A[R19]; Scer\GAL4[Tre.PU] rescues the reduced trehalose uptake phenotype of G-sα60A[R19]/+ flies fed on 10-40mM trehalose. Stimulation of long labellar taste bristles in G-sα60A[R19]/Scer\GAL4[Tre.PU]; G-sα60A[Scer\UAS.cCa] flies with trehalose induces significantly more biphasic spikes in associated sensory neurons than seen in G-sα60A[R19]/+ flies but not significantly more than that seen in wild-type flies.
When G-sα60AScer\UAS.cCa is driven by Scer\GAL4B185 the synaptic bouton and the late larval lethality phenotypes of G-sα60AB19 homozygotes. When driven by Scer\GAL4C380 or Scer\GAL4elav.PLu the neuromuscular junction phenotypes are rescued to wild-type. When driven by Scer\GAL4C57 or Scer\GAL4Mhc.PW in muscles, the defects in bouton formation is rescued, while the branching defects are only partially rescued.
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
Saccharomyces cerevisiae UAS construct a of Connolly
|Secondary FlyBase IDs|
|References ( 13 )|