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General Information
Symbol
Dmel\GαsUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Connolly
FlyBase ID
FBal0058938
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Gαs
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A cDNA encoding the short form of Gαs is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of G-sα60AScer\UAS.cCa under the control of Scer\GAL4P2.4.Pdf significantly reduces the response of the M pacemaker neurons in the brain to Pdf and to Dh31.

Expression of G-sα60AScer\UAS.cCa under the control of Scer\GAL4Mai179 significantly reduces the response of the E pacemaker neurons in the brain to Pdf.

Over 60% of flies expressing G-sα60AScer\UAS.cCa under the control of Scer\GAL4P0.5.Pdf in constant darkness are arrhythmic. While the remaining rhythmic flies exhibit normal periods, they are only weakly rhythmic, as shown by a lower rhythm power than control flies.

Expression of G-salpha60AScer\UAS.cCa under the control of either Scer\GAL4c309, Scer\GAL4247 (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4Tab2-201Y (in the γ-lobes) has no effect on spontaneous odor intensity or identity discrimination, nor conditioned odor intensity and identity discrimination.

Expression of G-sα60AScer\UAS.cCa under the control of Scer\GAL4c205 has no effect on visual pattern memory.

When expression of G-sα60AScer\UAS.cCa is driven in the dopamine and serotonin neurons by Scer\GAL4Ddc.PL, flies display a reduced responsiveness to an initial dose of cocaine compared to wild-type. Also no sensitization was seen on repeated exposures to cocaine, in contrast to wild-type. Flies expressing G-iα65AScer\UAS.cLa under the control of Scer\GAL4Ddc.PL show significantly decreased locomotor responses to the dopamine D2-like agonist quinpirole compared to controls. Flies expressing G-iα65AScer\UAS.cLa under the control of Scer\GAL4Ddc.PL exhibit no overt behavioural or lethality phenotypes are seen.

When expression is driven by Scer\GAL4238Y, Scer\GAL4c309, Scer\GAL4c747 or Scer\GAL4Tab2-201Y no effect on learning is seen.

Does not produce a visible phenotype when expressed using Scer\GAL430A.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of PdfrScer\UAS.cMa fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing G-sα60AScer\UAS.cCa under the control of Scer\GAL4P2.4.Pdf.

Co-expression of Dh31-R1Scer\UAS.cDa fully rescues the response of the M pacemaker neurons in the brain to Dh31 to wild-type levels in flies expressing G-sα60AScer\UAS.cCa under the control of Scer\GAL4P2.4.Pdf.

The increase in food consumption compared to controls seen in adults expressing sNPF-RScer\UAS.cLa under the control of Scer\GAL4sNPF-R.2.5 is suppressed by co-expression of GαsScer\UAS.cCa.

Co-expression of Gr21aScer\UAS.cKa and Gr63aScer\UAS.cKa under the control of Scer\GAL4Or22a.8010 in the "empty neuron" system ("Δhalo" embryos - Dp(2;2)dppd21 + Df(2L)dp-79b - in which Or22a and Or22b are deleted) confers a modest response to CO[[2]] in the ab3A olfactory receptor neuron (ORN). Additional co-expression of G-sα60AScer\UAS.cCa does not increase the response of the ab3A ORN to CO[[2]].

Animals co-expressing G-sα60AScer\UAS.cCa and AdoRScer\UAS.cDa under the control of Scer\GAL4en-e16E show 20% lethality as pharate adults, whereas expression of either G-sα60AScer\UAS.cCa or AdoRScer\UAS.cDa alone under the control of Scer\GAL4en-e16E does not cause lethality. The flies that do emerge show strong wing deformation and melanisation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of G-sα60AScer\UAS.cCa under the control of Scer\GAL4Orco.T:Hsim\VP16 in homozygous G-sα60AR60 ab3A olfactory receptor neurons rescues the reduced odour response seen in these neurons at near saturating concentrations of either methyl butyrate or butyric acid.

G-sα60AR19; Scer\GAL4Tre.PU rescues the reduced trehalose uptake phenotype of G-sα60AR19/+ flies fed on 10-40mM trehalose.

Stimulation of long labellar taste bristles in G-sα60AR19/Scer\GAL4Tre.PU; G-sα60AScer\UAS.cCa flies with trehalose induces significantly more biphasic spikes in associated sensory neurons than seen in G-sα60AR19/+ flies but not significantly more than that seen in wild-type flies.

When G-sα60AScer\UAS.cCa is driven by Scer\GAL4B185 the synaptic bouton and the late larval lethality phenotypes of G-sα60AB19 homozygotes. When driven by Scer\GAL4C380 or Scer\GAL4elav.PLu the neuromuscular junction phenotypes are rescued to wild-type. When driven by Scer\GAL4C57 or Scer\GAL4Mhc.PW in muscles, the defects in bouton formation is rescued, while the branching defects are only partially rescued.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
G-sα60AScer\UAS.cCa
G-sα60AUAS.cCa
GαsScer\UAS.cCa
GαsUAS.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Connolly
Secondary FlyBase IDs
    References (14)