Allele Dmel\Gαs^Scer\UAS.cCa

General Information
Symbol	Dmel\Gαs^Scer\UAS.cCa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Connolly	FlyBase ID	FBal0058938
Feature type	allele	Associated gene	Dmel\Gαs

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Wolfgang et al., 1996, Connolly et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A cDNA encoding wild type G-sα60A is expressed from Scer\UAS regulatory sequences. (Connolly et al., 1996) Construct: cDNA encoding the short form of G-sα60A is expressed under the control of Scer\UAS regulatory sequences. (Wolfgang et al., 1996)
Carried in construct	P{UAS-G-sα60A.C} (Liu et al., 2006, Kimura et al., 2004, Wolfgang et al., 2004, Li et al., 2000, Connolly et al., 1996, Wolfgang et al., 1996, Dolezelova et al., 2007, Ueno et al., 2006, Xia and Tully, 2007, Dahdal et al., 2010, Yao and Carlson, 2010, Duvall and Taghert, 2012, Deng et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	circadian rhythm defective, with Scer\GAL4^P0.5.Pdf (Dahdal et al., 2010)
Phenotype Manifest In
	labellar taste bristle, with Scer\GAL4^Gr5a.PU (Ueno et al., 2006) large labellar taste bristle, with Scer\GAL4^Gr5a.PU (Ueno et al., 2006)
Detailed Description
	Statement Reference Expression of G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P2.4.Pdf] significantly reduces the response of the M pacemaker neurons in the brain to Pdf and to Dh31. Expression of G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[Mai179] significantly reduces the response of the E pacemaker neurons in the brain to Pdf. (Duvall and Taghert, 2012) Over 60% of flies expressing G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P0.5.Pdf] in constant darkness are arrhythmic. While the remaining rhythmic flies exhibit normal periods, they are only weakly rhythmic, as shown by a lower rhythm power than control flies. (Dahdal et al., 2010) Expression of G-salpha60A[Scer\UAS.cCa] under the control of either Scer\GAL4[c309], Scer\GAL4[247] (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4[Tab2-201Y] (in the γ-lobes) has no effect on spontaneous odor intensity or identity discrimination, nor conditioned odor intensity and identity discrimination. (Xia and Tully, 2007) Expression of G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^c205 has no effect on visual pattern memory. (Liu et al., 2006) G-sα60A^Scer\UAS.cCa; Scer\GAL4^en-e16E adults have normal wings. (Kimura et al., 2004) When expression of G-sα60A^Scer\UAS.cCa is driven in the dopamine and serotonin neurons by Scer\GAL4^Ddc.PL, flies display a reduced responsiveness to an initial dose of cocaine compared to wild-type. Also no sensitization was seen on repeated exposures to cocaine, in contrast to wild-type. Flies expressing G-iα65A^Scer\UAS.cLa under the control of Scer\GAL4^Ddc.PL show significantly decreased locomotor responses to the dopamine D2-like agonist quinpirole compared to controls. Flies expressing G-iα65A^Scer\UAS.cLa under the control of Scer\GAL4^Ddc.PL exhibit no overt behavioural or lethality phenotypes are seen. (Li et al., 2000) When expression is driven by Scer\GAL4^238Y, Scer\GAL4^c309, Scer\GAL4^c747 or Scer\GAL4^Tab2-201Y no effect on learning is seen. (Connolly et al., 1996) Does not produce a visible phenotype when expressed using Scer\GAL4^30A. (Wolfgang et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference AdoR^Scer\UAS.cDa, Gαs^Scer\UAS.cCa, Scer\GAL4^en-e16E has lethal \| pharate adult stage phenotype (Dolezelova et al., 2007) AdoR^Scer\UAS.cDa, Gαs^Scer\UAS.cCa, Scer\GAL4^en-e16E has visible phenotype (Dolezelova et al., 2007)
Phenotype Manifest In
Other
Statement Reference AdoR^Scer\UAS.cDa, Gαs^Scer\UAS.cCa, Scer\GAL4^en-e16E has wing phenotype (Dolezelova et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Pdfr[Scer\UAS.cMa] fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P2.4.Pdf]. Co-expression of Dh31-R1[Scer\UAS.cDa] fully rescues the response of the M pacemaker neurons in the brain to Dh31 to wild-type levels in flies expressing G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[P2.4.Pdf]. (Duvall and Taghert, 2012) Co-expression of Gr21a[Scer\UAS.cKa] and Gr63a[Scer\UAS.cKa] under the control of Scer\GAL4[Or22a.8010] in the "empty neuron" system ("Δhalo" embryos - Dp(2;2)dpp[d21] + Df(2L)dp-79b - in which Or22a and Or22b are deleted) confers a modest response to CO[[2]] in the ab3A olfactory receptor neuron (ORN). Additional co-expression of G-sα60A[Scer\UAS.cCa] does not increase the response of the ab3A ORN to CO[[2]]. (Yao and Carlson, 2010) Animals co-expressing G-sα60A[Scer\UAS.cCa] and AdoR[Scer\UAS.cDa] under the control of Scer\GAL4[en-e16E] show 20% lethality as pharate adults, whereas expression of either G-sα60A[Scer\UAS.cCa] or AdoR[Scer\UAS.cDa] alone under the control of Scer\GAL4[en-e16E] does not cause lethality. The flies that do emerge show strong wing deformation and melanisation. (Dolezelova et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Gαs^Scer\UAS.cCa/Scer\GAL4^B185 rescues Gαs^B19 (Wolfgang et al., 2004) Gαs^Scer\UAS.cCa/Scer\GAL4^Gr5a.PU rescues Gαs^R19/G-salpha60A[+] (Ueno et al., 2006) Gαs^Scer\UAS.cCa/Scer\GAL4^{Orco.T:Hsim\VP16} rescues Gαs^R60 (Yao and Carlson, 2010)
Partially rescues	Gαs^Scer\UAS.cCa/Scer\GAL4^C57 partially rescues Gαs^B19 (Wolfgang et al., 2004) Gαs^Scer\UAS.cCa/Scer\GAL4^elav.PLu partially rescues Gαs^B19 (Wolfgang et al., 2004) Gαs^Scer\UAS.cCa/Scer\GAL4^Mhc.PW partially rescues Gαs^B19 (Wolfgang et al., 2004) Scer\GAL4^futsch-C380/Gαs^Scer\UAS.cCa partially rescues Gαs^B19 (Wolfgang et al., 2004)
Comments	Expression of G-sα60A[Scer\UAS.cCa] under the control of Scer\GAL4[Orco.T:Hsim\VP16] in homozygous G-sα60A[R60] ab3A olfactory receptor neurons rescues the reduced odour response seen in these neurons at near saturating concentrations of either methyl butyrate or butyric acid. (Yao and Carlson, 2010) G-sα60A[R19]; Scer\GAL4[Tre.PU] rescues the reduced trehalose uptake phenotype of G-sα60A[R19]/+ flies fed on 10-40mM trehalose. Stimulation of long labellar taste bristles in G-sα60A[R19]/Scer\GAL4[Tre.PU]; G-sα60A[Scer\UAS.cCa] flies with trehalose induces significantly more biphasic spikes in associated sensory neurons than seen in G-sα60A[R19]/+ flies but not significantly more than that seen in wild-type flies. (Ueno et al., 2006) When G-sα60A^Scer\UAS.cCa is driven by Scer\GAL4^B185 the synaptic bouton and the late larval lethality phenotypes of G-sα60A^B19 homozygotes. When driven by Scer\GAL4^C380 or Scer\GAL4^elav.PLu the neuromuscular junction phenotypes are rescued to wild-type. When driven by Scer\GAL4^C57 or Scer\GAL4^Mhc.PW in muscles, the defects in bouton formation is rescued, while the branching defects are only partially rescued. (Wolfgang et al., 2004)
Stocks ( 2 )
Bloomington	6489 w^*; P{UAS-G-sα60A.C}9
Kyoto	108804 w^*; P{UAS-G-sα60A.C}9
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	G-sα60A^Scer\UAS.cCa G-sα60A^UAS.cCa Gαs^Scer\UAS.cCa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Connolly (Connolly et al., 1996)
Secondary FlyBase IDs

References ( 13 )
Research paper	Duvall and Taghert, 2012, PLoS Biol. 10(6): e1001337 The Circadian Neuropeptide PDF Signals Preferentially through a Specific Adenylate Cyclase Isoform AC3 in M Pacemakers of Drosophila. [FBrf0218594] Deng et al., 2011, PLoS ONE 6(4): e18605 The Stimulatory Gα(s) Protein Is Involved in Olfactory Signal Transduction in Drosophila. [FBrf0213437] Dahdal et al., 2010, Neuron 68(5): 964--977 Drosophila Pacemaker Neurons Require G Protein Signaling and GABAergic Inputs to Generate Twenty-Four Hour Behavioral Rhythms. [FBrf0212523] Yao and Carlson, 2010, J. Neurosci. 30(13): 4562--4572 Role of G-proteins in odor-sensing and CO2-sensing neurons in Drosophila. [FBrf0210441] Dolezelova et al., 2007, Insect Biochem. Mol. Biol. 37(4): 318--329 A Drosophila adenosine receptor activates cAMP and calcium signaling. [FBrf0201557] Xia and Tully, 2007, PLoS Biol. 5(10): e264 Segregation of odor identity and intensity during odor discrimination in drosophila mushroom body. [FBrf0202823] Liu et al., 2006, Nature 439(7076): 551--556 Distinct memory traces for two visual features in the Drosophila brain. [FBrf0191247] Ueno et al., 2006, J. Neurosci. 26(23): 6143--6152 Gsalpha is involved in sugar perception in Drosophila melanogaster. [FBrf0195217] Kimura et al., 2004, Development 131(7): 1597--1606 Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster. [FBrf0174562] Wolfgang et al., 2004, Dev. Biol. 268(2): 295--311 Signaling through Gs alpha is required for the growth and function of neuromuscular synapses in Drosophila. [FBrf0174523] Li et al., 2000, Curr. Biol. 10(4): 211--214 Ectopic G-protein expression in dopamine and serotonin neurons blocks cocaine sensitization in Drosophila melanogaster. [FBrf0127196] Connolly et al., 1996, Science 274(5295): 2104--2107 Associative learning disrupted by impaired Gs signaling in Drosophila mushroom bodies. [FBrf0091035] Wolfgang et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 93(25): 14542--14547 Activation of protein kinase A-independent pathways by Gs alpha in Drosophila. [FBrf0090863]

Allele Dmel\GαsScer\UAS.cCa

Allele Dmel\Gαs^Scer\UAS.cCa