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General Information
Symbol
Dmel\GαsQ215L.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0058939
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-GαsQ215L
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q215L. Impairs the GTPase activity resulting in a molecule that constitutively activates adenyl cyclase.

An activated form of Gαs (contains the acid replacement: Q215L) is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When driven by Scer\GAL4elav-C155, overexpression of G-sα60AQ215L.Scer\UAS does not alter neuromuscular junction morphology.

Over 60% of flies expressing G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4P0.5.Pdf in constant darkness are arrhythmic. While the remaining rhythmic flies exhibit normal periods, they are only weakly rhythmic, as shown by a lower rhythm power than control flies.

The response of the ab1C neuron to CO[[2]] is unaffected in animals expressing G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4Gr21a.9.323.

The odour responses of the ab1A, ab2A and ab3A olfactory receptor neurons are unaffected in animals expressing G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4Orco.T:Hsim\VP16.

Transient expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4hs.PU using heat shock results does not affect the spontaneous firing rate of the ab1C or ab3A ORNs compared to non-heat shocked controls.

Expression of G-salpha60AQ215L.Scer\UAS under the control of either Scer\GAL4c309, Scer\GAL4247 (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4Tab2-201Y (in the γ-lobes) leaves spontaneous odor intensity discrimination and avoidance of individual odors unchanged.

Expression of G-salpha60AQ215L.Scer\UAS under the control of either Scer\GAL4c309, Scer\GAL4247 (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4Tab2-201Y (in the γ-lobes) has no effect on spontaneous odor identity discrimination, nor conditioned odor intensity. However, conditioned odor identity discrimination is severely affected. This discrimination is abolished in a Scer\GAL80ts.αTub84B background at 30[o]C.

Expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4c205 abolishes memory of the visual pattern element "elevation", but leaves memory of "contour orientation" intact. The abolition of memory of the "elevation" pattern parameter is also seen in flies in which G-sα60AQ215L.Scer\UAS is expressed under the control of Scer\GAL4c205 only in the adult (in this case Scer\GAL80ts.αTub84B is used to block Scer\GAL4c205 function throughout development, but it is then inactivated by raising adult flies at 30oC, allowing Scer\GAL4c205 to drive expression of G-sα60AQ215L.Scer\UAS in the adults).

G-sα60AQ215L.Scer\UAS; Scer\GAL4en-e16E adults have blistered wings. There is precocious cell of wing blade cells in late stage pharate adults (G+; <3hrs before eclosion) of this genotype. However, wing cuticle structure is normal.

Expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4OK10 results in wing blistering, shortening of the scutellum and crossing and inappropriate anterior orientation of the posterior scutellar bristles.

When expression is driven by Scer\GAL4238Y, Scer\GAL4c309 or Scer\GAL4c747 learning is abolished. When expression is driven by Scer\GAL4Tab2-201Y learning is reduced by 50%. Olfactory responses to OCT and MCH are normal, as were responses to electroshock. When expression is driven by Scer\GAL4Aph-4-c232 or Scer\GAL4OK348 learning is not affected. When expression is driven by Scer\GAL41407 causes neither lethality nor overt behavioral defects.

The dorsal and ventral surfaces of the wing do not adhere to each other and separate following emergence from the pupal case, producing wing blisters, when G-sα60AQ215L.Scer\UAS is expressed using Scer\GAL430A or Scer\GAL4OK10.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Overexpression of G-sα60AQ215L.Scer\UAS completely suppresses the neuromuscular junction (NMJ) phenotype of Df(1)Exel9051/Y.

The arrhythmicity caused by expression of G-sα60AQ215L.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf in constant darkness is completely suppressed by co-expression of dncScer\UAS.cCa. These flies also exhibit ~2hr shorter periods than flies expressing dncScer\UAS.cCa alone.

Df(2L)wg-CX3/+ weakly suppresses the phenotype caused by expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4OK10; the scutellum and posterior scutellar bristles are normal, but the wings remain blistered. The phenotype caused by expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4OK10 is suppressed almost to wild type by either Df(2L)osp29/+ or Df(3L)vin7/+. gft06430/+ moderately suppresses the phenotype caused by expression of G-sα60AQ215L.Scer\UAS under the control of Scer\GAL4OK10; wing blistering is reduced.

Blisters are also produced when G-sα60AQ215L.Scer\UAS is expressed using Scer\GAL430A in a vn1 rhove-1 double mutant background. Reduction or elimination of protein kinase A activity has no effect on the phenotype generated when the G-sα60A pathway is activated in wing epithelial cells; wing duplications that contain blisters are formed when Pka-R1BDK.Scer\UAS and G-sα60AQ215L.Scer\UAS are co-expressed using Scer\GAL430A, and when G-sα60AQ215L.Scer\UAS is expressed in a Pka-C1H2 background using Scer\GAL4OK10.

Xenogenetic Interactions
Statement
Reference

The arrhythmicity caused by expression of G-sα60AQ215L.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf in constant darkness is not suppressed by co-expression of Mmus\Cd8aScer\UAS.T:Avic\GFP.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
G-sα60AQ215L.Scer\UAS
G-sα60AQ215L.UAS
GαsQ215L.Scer\UAS
GαsQ215L.UAS
Name Synonyms
Secondary FlyBase IDs
    References (14)