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General Information
Symbol
Dmel\EgfrDN.UAS
Species
D. melanogaster
Name
dominant negative
FlyBase ID
FBal0058990
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-EGFRDN, UAS-DN-DER, UAS-DN-EGFR, UAS-EgfrDN, EGFRDN, UAS-EGFR.DN, UAS-DERDN, UAS-DNDER, DERDN, UAS-DNEgfr
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a dominant-negative form of Egfr that consists of a type I Egfr cDNA with a termination codon 13 amino acids C-terminal to the transmembrane domain.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

antenna & neuron | conditional ts, with Scer\GAL4hs.PB

border follicle cell & filopodium, with Scer\GAL4slbo.2.6

chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4sca-109-68

embryonic trachea & cortical actin cytoskeleton, with Scer\GAL4btl.PS

sensory neuron & axon & embryo, with Scer\GAL4repo

Detailed Description
Statement
Reference

Expressing EgfrDN.UAS under the control of Scer\GAL4C587 induces non-enclosing somatic cells and supernumerary early germline cells in L3 male gonads.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 does not significantly alter the protrusion and retraction morphodynamics in border cell cluster migration.

Scer\GAL4C855a-mediated expression of EgfrDN.Scer\UAS results in reduction in neuroepithelium size and formation of fewer neuroblasts.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4Bx-MS1096 results in a partial loss of wing veins L3, L4 and L5.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 has little effect on the speed of net cluster movement in the early phase of posterior border cell migration, but movement in the late phase is reduced compared to controls, enhancing the difference between the two phases. The mutant cells show increased tumbling in the late phase compared to controls and the number of cellular extensions from the front of the border cell cluster is reduced compared to wild type.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF produces a rough eye phenotype.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4Ilp2.PR results in flies with increased sensitivity to ethanol-induced sedation compared to control flies.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4P2.4.Pdf prevents increases in sleep after social enrichment.

Scer\GAL4GMR.PF-mediated expression of EgfrDN.Scer\UAS results in eyes that are severely reduced in size and mostly devoid of ommatidial organization.

Flip-out clones expressing EgfrDN.Scer\UAS in the wing, under the control of Scer\GAL4Act5C.PI interrupts wing veins LE, L3, and L4.

Expression of EgfrDN.Scer\UAS in the wing using Scer\GAL4Bx-MS1096 results in dramatically reduced, narrow wings and loss of wing veins.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

Animals expressing EgfrDN.Scer\UAS under the control of Scer\GAL4so-7.GAL4 develop only 3 or 4 immature photoreceptors in the Bolwig's organ compared to approximately 12 cells in the wild type. The remaining photoreceptors in the mutant larvae are all of the Rh5 subtype, no Rh6 photoreceptors are present.

The tracheal systems of embryos that express EgfrDN.Scer\UAS under the control of Scer\GAL4btl.PS show branch interruptions and branches with cells only connected by cytoplasmic extensions. This phenotype has a 96% penetrance. The general pattern and outgrowth of dorsal and ganglionic branches is not grossly affected.

The tracheal tubes of stage 16 Scer\GAL4btl.PS>EgfrDN.Scer\UAS embryos show a thinner accumulation of cortical actin compared to controls.

Expression of EgfrDN.Scer\UAS, under the control of Scer\GAL4GMR.PF, suppresses wing vein formation in the wing.

There is a large increase in primordial germ cell numbers when the dominant negative EgfrDN.Scer\UAS is expressed in somatic gonadal cells by Scer\GAL4C587.

Fewer intermingled cells (somatic cells adjacent to primordial germ cells) are present in EgfrDN.Scer\UAS mutants (driven by Scer\GAL4C587) gonads compared to wild-type.

There is a significant increase in apoptosis in late 2nd instar larva expressing EgfrDN.Scer\UAS under the control of Scer\GAL4C587, compared to larva expressing Scer\GAL4C587 alone. Other aspects of somatic differentiation and morphogenesis seem normal in these mutants.

Overexpression of EgfrDN.Scer\UAS under the control of Scer\GAL4796 decreases cell size but does not affect synapse number.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 does not affect border cell migration.

Expression of EgfrDN.Scer\UAS, under the control of Scer\GAL4en-e16E, in the posterior compartment cells of embryos results in apoptosis of these cells, beginning at stage 12. This increased cell death reduces the number of cells per posterior compartment from 44 to 30 and compartment size is reduced by 15%. The increased apoptosis occurs mainly at the front of the posterior compartment. Surviving cells are larger than normal.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4ato.3.6 does not affect dorsal cluster neuron axon extension across the optic chiasm in adult flies.

Ommatidia that express EgfrDN.Scer\UAS, under the control of Scer\GAL4GMR.PF, show differentiation of only the first two or three photoreceptors (most likely to be R8 and R2 or R5), with others failing to differentiate.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4gcm-rA87.C blocks the differentiation of L1-L5 lamina neurons.

Hemocytes in EgfrDN.Scer\UAS; Scer\GAL4srp.Hemo late embryos do not exhibit abnormal aggregation.

Migration many ganglionic branches approaching the midline during stage 16 is defective in EgfrDN.Scer\UAS; Scer\GAL4bs-23.26 embryos: 9% of branches are stalled and 25% misrouted, 5% cross the midline and 3% arrest upon reaching it.

lch5 ligament attachment cells are not formed in EgfrDN.Scer\UAS; Scer\GAL469B or EgfrDN.Scer\UAS; Scer\GAL4sr-md710 embryos. lch5 cap attachment cells, however, are unaffected.

When EgfrDN.Scer\UAS is driven by Scer\GAL4hs.PB and heatshocked an alteration of the fascicular pattern of the sensory afferents from the antenna are seen.

P{UAS-Egfr.DN}29-77-1; Scer\GAL4prd.RG1 embryos do not have obvious cuticle phenotypes. However, P{UAS-Egfr.DN}1-7; Scer\GAL4prd.RG1 embryo cuticles have in strong denticle belt fusions in alternating parasegments, centred on the midline and extending up to 2/3 of the width of each denticle belt. From stages 10-12 there is a dramatic increase in apoptosis in prd expression domains in the developing epidermis of these animals. Increased apoptosis persists at the midline through stages 13 and 14.

When EgfrDN.Scer\UAS is driven by Scer\GAL4hs.PB adults exhibit misrotated ommatidia.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

EgfrDN.Scer\UAS; Scer\GAL4Ubx-lac1-Gal4 adults exhibit wing duplication in the posterior compartment and partial loss of notum and/or hinge. Occasionally pharate adults die within the pupal case, with severe loss of notum tissue.

Expression of EgfrDN.Scer\UAS in embryonic glia, under the control of Scer\GAL4repo, causes PNS patterning defects in embryonic hemisegments. In hemisegments that are moderately affected, sensory axons can be misdirected and nerves in the CNS-PNS transition zone show incorrect fasciculation-bundling. In severely-affected hemisegments, there is a loss of sensory neurons and those remaining have misplaced cell bodies and misdirected sensory axons. Additionally, motor neuron patterning is disrupted.

In EgfrDN.Scer\UAS; Scer\GAL4sli.PS embryos midline glial cells form normally, but only a few survive to the end of embryogenesis.

When EgfrDN.Scer\UAS is driven by Scer\GAL4twi.PB a reduction in the number of DA1 and DO2 muscles.

Border cells in the ovaries of EgfrDN.Scer\UAS; Scer\GAL4slbo.2.6 animals form significantly fewer long cellular extensions (20-40 μm) and intermediate cellular extensions (10-20 μm).

Expression of btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4sca-537.4 or Scer\GAL4Dll-md23 has no detectable effect on bract formation. When driven by Scer\GAL4sca-537.4 other defects are seen. Tarsal segments 2-4 are shortened and fused, and the entire tarsus is reduced to a bump on the end of a swollen tibia. When driven by Scer\GAL4Dll-md23, no adult survivors are seen.

Dorsal migration of border cells is severely inhibited when EgfrDN.Scer\UAS is expressed under the control of either Scer\GAL4slbo.2.6, with only minor effects on the initial posterior migration. Most eggs derived from these females do not hatch.

When EgfrDN.Scer\UAS is driven by Scer\GAL4slbo.2.6 a mild effect on border cell migration is seen.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4en-e16E at 25oC or 29oC results in a complete loss of oenocytes. At 25oC, the number of chordotonal organs in the lateral cluster is reduced from 5 to 4 in these embryos, while at 29oC the number is reduced from 5 to 3. Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4salm-459.2 at 29oC has no effect on chordotonal organ number but does result in a significant reduction in the number of oenocytes per cluster.

The first longitudinal fascicle in the embryonic central nervous system of EgfrDN.Scer\UAS; Scer\GAL4elav.PLu embryos is misrouted across the midline, but the second and third longitudinal fascicles are normal. Development of the longitudinal (interface) glial cells is normal. EgfrDN.Scer\UAS; Scer\GAL4htl.POS embryos exhibit increased apoptosis of longitudinal (interface) glial cells.

Flies expressing EgfrDN.Scer\UAS under the control of Scer\GAL4Act5C.PI lack the eye and antenna.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4dpp.blk1 inhibits furrow reincarnation along the lateral margins, but not furrow birth at the posterior of the eye disc.

Neuronal patterning in the ommatidial preclusters is maintained in flies expressing EgfrDN.Scer\UAS under the control of Scer\GAL4lz-gal4.

When EgfrDN.Scer\UAS is driven by Scer\GAL4sca-P309 or Scer\GAL4lz-gal4 a significant increase in coeloconic sensilla and a concomitant reduction of basiconica and trichoidea is seen on the antennal surface of these animals.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4Dll-md23 interferes with leg disc development in embryos, while leaving the wing disc intact.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL415J2 does not result in consistent errors in axon trajectory or longitudinal tract establishment.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF leads to complete loss of the adult retina. In animals raised at 29[o]C, veins L2, L3, L4 and L5 are disrupted.

Most animals expressing EgfrDN.Scer\UAS under the control of Scer\GAL4sca-537.4 die as embryos, except for a few survivors to the larval stage.

Eggs derived from females expressing EgfrDN.Scer\UAS under the control of Scer\GAL4T155 show a ventralisation of the eggshell.

When expression is driven by Scer\GAL4T155 the pleura expands at the expense of the sternites and tergites.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4A9 results in a dramatic reduction in the dorsal compartment of the wing pouch in the wing disc.

EgfrDN.Scer\UAS driven by either Scer\GAL4e22c or Scer\GAL469B results in extra naked cuticle in otherwise wild-type embryos.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4sca-109-68 results in a dramatic loss of chordotonal sensory organ precursors (SOPs) in late third instar imaginal leg discs. The appearance of bristle SOPs is unaffected. Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4ase.PZ has no effect on the number of ase-expressing SOPs in the leg disc.

When expression is driven by Scer\GAL4αTub84B.PP in clones in the lamina, timed to permit imaginal cell proliferation and the formation of photoreceptor clusters at the posterior of the eye, an essentially normal array of photoreceptors innervate the lamin target field. However neuronal differentiation in the LPCs fails.

When expression is driven in the embryo with Scer\GAL4hs.PB ectopic apoptosis is induced.

When expressed under the influence of Scer\GAL448Y, cells in the endoderm are missing or unhealthy in the lab domain and the anterior midgut in the ps3/ps4 region. The midgut epithelium is largely intact. When expressed under the influence of Scer\GAL4how-24B, midgut constrictions are abnormal and the gastric caeca are stunted.

DA1 muscle precursor formation is either normal or affected in not more than one segment in embryos expressing EgfrDN.Scer\UAS under the control of Scer\GAL4how-24B at 22oC.

Embryos expressing EgfrDN.Scer\UAS under the control of Scer\GAL4en-e16E lack the first denticle row.

Scer\GAL4sli.PS-mediated expression in wild type embryos does not result in an abnormal CNS phenotype. Scer\GAL4sim.PS-mediated expression causes a weak fused commissure phenotype.

Produces severely mutant larvae that do not hatch from their vitelline membranes when expressed ubiquitously in embryos using Scer\GAL4arm.PS. These larvae have head defects and very narrow denticle belts. The abdominal belts usually consist of approximately 4 disordered rows of denticles, which are tapered and are less hooked compared to wild-type. The belts normally contain one or two anterior rows with a few largish denticles, followed by a strip of tiny denticles. The denticles correspond to row 5 and 6 denticles. The second and third thoracic denticle belts also lack most of their denticles, although the prothoracic denticle belt and beard appear normal.

Scer\GAL4GMR.PF induced expression causes complete loss of the adult retina, some interommatidial bristles remain. Photoreceptor development is disrupted and the eye-antennal disc shows an overall disorganisation. Scer\GAL4hs.PB induced expression prevents receptor cell R7 in some ommatidia from forming. Early pupal heat shocks causes ommatidia that have 2, instead of 4 cone cells, and are depleted of primary, secondary and tertiary pigment cells, independent of the number of photoreceptor cells. Scer\GAL4hs.2sev induced expression prevents receptor cells R1, R3, R4, R6 and R7 from forming.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference

EgfrDN.UAS, Scer\GAL4slbo.2.6 is an enhancer of border follicle cell & filopodium phenotype of PvrDN.UASp, Scer\GAL4slbo.2.6

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Scer\GAL4arm.PS/EgfrDN.UAS is a non-suppressor of denticle phenotype of wgl-17

Other
Additional Comments
Genetic Interactions
Statement
Reference

Flies expressing EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show differences in the migration behaviour of border cell clusters. First, a distinct front-back polarity is evident in wild-type clusters, which show spatially segregated protrusion and retraction with high protrusion velocities predominating at the front and high retraction velocities at the back. This spatial segregation is lost in clusters lacking guidance-receptor activities. Second, these double mutant clusters display overall slower protrusion and retraction velocities.

Flies expressing Rac1N17.Scer\UAS, PvrDN.Scer\UAS and EgfrDN.Scer\UAS exhibit a change in border cell migration, with changes in local protrusion and retraction behaviour that are different to those found in Rac1N17.Scer\UAS single mutants.

Flies expressing Rac1N17.Scer\UAS and EgfrDN.Scer\UAS exhibit a change in border cell migration, with changes in local protrusion and retraction behaviour that are different to those found in Rac1N17.Scer\UAS single mutants.

The addition of a dominant-negative TieDN.Scer\UAS to border cells expressing PvrDN.Scer\UAS and EgfrDN.Scer\UAS alters the protrusion and retraction morphodynamics of border cell cluster migration.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4esg.PU suppresses the intestinal stem cell hyperproliferation seen in BursZ5569/BursZ5569 mutants.

Downregulation of Egfr, through expression of EgfrDN.Scer\UAS, suppresses the Scer\GAL4GMR.PU>miple1Scer\UAS.T:Ivir\HA1 eye phenotype.

Downregulation of Egfr, through expression of EgfrDN.Scer\UAS, does not alter the ommatidial rotation phenotype seen in flies expressing miple1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.2sev.

Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4Act.PU suppresses the increased size of Apc2g10 ApcQ8 mutant intestinal stem cell clones 7 and 14 days after clone induction (ACI). The multilayering phenotype often seen at 21 days ACI is also suppressed.

Co-expression of PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in defects in border cell migration.

Border cell clusters show severe delays in initiating posterior migration and show strongly reduced forward speed once migratory in females co-expressing PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6. The number of cellular extensions from the front of the border cell cluster is reduced compared to wild type and an increase in side and back extensions.

A homozygous rho-5KO1 background suppresses the rough eye phenotype seen when EgfrDN.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

Expression of EgfrDN.Scer\UAS in somatic muscle cells (under the control of Scer\GAL4Mef2.PR) of sli2 mutants decreases the size and/or number of adhesion sites along the midline.

Coexpression of EgfrDN.Scer\UAS with vg2.Scer\UAS under the control of Scer\GAL4Mef2.PR results in the size and number of the ectopic muscle adhesion sites being decreased compared to when vg2.Scer\UAS alone is expressed and increased compared to EgfrDN.Scer\UAS lone expression.

Coexpression of tkvQ253D.Scer\UAS.cNb with EgfrDN.Scer\UAS, under the control of Scer\GAL4e22c, not only fails to induce ectopic vein cells, but also inhibits endogenous vein formation, suggesting that activation of the dpp pathway alone is not sufficient for vein differentiation.

A CblK26 heterozygous background rescues the rough eye phenotype found in mutants expressing EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF.

Coexpression of EgfrDN.Scer\UAS and PvrDN.Scer\UAS, under the control of Scer\GAL4slbo.2.6, results in a strong enhancement of the border cell misguidance phenotype seen when PvrDN.Scer\UAS is expressed alone; the penetrance of the phenotype is increased from 16 to 90%.

Coexpression of EgfrDN.Scer\UAS and CycEScer\UAS.cLa, under the control of Scer\GAL4en-e16E, show approximately double the amount of apoptosis in cells of posterior compartments relative to expressing CycEScer\UAS.cLa alone.

Co-expression of PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4c306 results in border cell migration defects. The severity of the migration defects is enhanced if the females also express TieDN.Scer\UAS.

Coexpression of the EgfrDN.Scer\UAS and mir-7Scer\UAS.cLb transgenes, under the control of Scer\GAL4GMR.PF, results in ommatidia with ectopic photoreceptor cells. The positions of these cells suggest they are R3 and R4.

Denticle belt fusions in the cuticles of rho7M43; ru1 double homozygous embryos are enhanced by P{UAS-Egfr.DN}29-77-1; Scer\GAL4prd.RG1.

Co-expression of spenDN.Scer\UAS suppresses the small eye phenotype caused by expression of EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF.

The loss of midline glial (MG) cells in EgfrDN.Scer\UAS; Scer\GAL4sli.PS embryos is suppressed by WrvX1/WrvX1. Approximately twice as many MG cells survive as in wild-type (average of 6.1 per segment n=201, compared to average = 2.8 for wild-type).

The addition of argosΔ7 or DlX to EgfrDN.Scer\UAS, Scer\GAL4twi.PB embryos suppresses their DO2 phenotype. The DA1 phenotype is also suppressed, by homozygous argosΔ7.

Border cells in the ovaries of EgfrDN.Scer\UAS; PvrDN.Scer\UAS; Scer\GAL4slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm).

EgfrDN.Scer\UAS and PvrDN.Scer\UAS, when driven by Scer\GAL4slbo.2.6, leads to a dramatic effect on border cell migration. 90% of border cell clusters migrate less than half way towards the oocyte. In 5% of egg chambers, border cell clusters are found off the direct track to the oocyte. The combination of Pvf1EP1624, and EgfrDN.Scer\UAS (driven by Scer\GAL4slbo.2.6) leads to strong effect. Border cells are not able to reach the oocyte by stage 10, and they also show a low level of "off track" migration.

Co-expression of CblDv.Scer\UAS with EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF suppresses the phenotypes of both mutants. Retinal development is restored although the eye is smaller than wild-type and ommatidial orientation is irregular. At 29[o]C, veins L2, L3 and L5 are completely rescued, with partial rescue of L4.

When co-expression of EgfrDN.Scer\UAS and panΔN.Scer\UAS is driven by Scer\GAL4T155 the entire tergite is transformed to pleura. When co-expression of EgfrDN.Scer\UAS and dppScer\UAS.cSa is driven by Scer\GAL4T155 the tergite is reduced to a very small patch near the dorsal midline and the lateral tergite is completely transformed to pleura. These effects are not due to reduced histoblast proliferation.

The addition of ovoScer\UAS.cPa suppresses the extra naked cuticle seen in embryos expressing EgfrDN.Scer\UAS driven by either Scer\GAL4e22c or Scer\GAL469B.

When EgfrDN.Scer\UAS is expressed in combination with dppScer\UAS.cSa under the influence of Scer\GAL4how-24B, gut morphology is more normal than for dppScer\UAS.cSa, Scer\GAL4how-24B mutants alone.

The loss of the DA1 muscle precursor in vn10567 embryos is enhanced if they are also carrying EgfrDN.Scer\UAS expressed under the control of Scer\GAL4how-24B.

Homozygous wgl-12 larvae carrying EgfrDN.Scer\UAS which have been shifted to the restrictive temperature after 8 hours of development have denticle belts with almost exclusively large denticles of the row 5 type (although their hooks somewhat resemble the row 1-4 type) and an apparently normal strip of row 6 denticles most posteriorly. Almost all the denticles point posteriorly. Does not alter the denticle phenotype of wgl-17 larvae when expressed in embryos under the control of Scer\GAL4arm.PS.

Xenogenetic Interactions
Statement
Reference

The effect on border cell cluster migration when all border cells co-express PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 and a single border cell expresses Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP have been studied. In those border cell clusters where the single Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Egfr::Hsap\EGFRhE-E.Scer\UAS.T:Avic\GFP-expressing cell is in the back, the cluster on average moves relatively more backwards.

In the absence of illumination, border cells expressing EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 and carrying Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 fail to move forward. When Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 is photoactivated at the rear of the border cell cluster, rearward movement is seen. When photoactivation is stopped, the border cell cluster stops moving.

Photo-inactivation of Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 at the front of border cell clusters expressing Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1, EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in local retraction.

Prevention of cell death in Scer\GAL4btl.PS>EgfrDN.Scer\UAS embryos, by coexpression of BacA\p35Scer\UAS.cHa, does not suppress the tracheal branching integrity phenotype.

The ectopic wing vein phenotype of Scer\GAL4GMR.PF>Hsap\ATN1N917.65Q.Scer\UAS flies is suppressed when EgfrDN.Scer\UAS is coexpressed. Wings of flies expressing both transgenes resemble wings that express only EgfrDN.Scer\UAS.

Coexpression of BacA\p35Scer\UAS.cHa and EgfrDN.Scer\UAS, under the control of Scer\GAL4en-e16E, in the posterior compartment cells of the embryo leads to a 14% reduction in the size of these cells.

Complementation and Rescue Data
Comments

Phenotype, when driven by Scer\GAL4GMR.PF can be rescued by overexpression of wild type Egfr (EgfrScer\UAS.cBa).

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Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
dominant negative
Secondary FlyBase IDs
    References (103)