Open Close
General Information
Symbol
Dmel\CycEUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lane
FlyBase ID
FBal0059170
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-CycE, UAS-Cyclin E, UAS-CycE.L
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of CycE.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval brains expressing CycEScer\UAS.cLa under the control of Scer\GAL4insc-Mz1407 do not show any significant change in the nucleolar size of immature secondary neuroblasts, as compared to controls.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4salm.EPv has no effect on wing or wing cell size.

Scer\GAL4en.PU-mediated expression reduces the fraction of wing disc cells in G1 phase and concomitantly increases the fraction in G2.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4en-e16E results in a drastic lowering of the number of cells in G[[1]] phase and an increase in the number of cells in S phase compared to controls, while the overall length of the cell cycle is similar to controls.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4en-e16E has no effect on the posterior wing compartment.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4en-e16E induces cells in the posterior compartment cells of stage 12 embryos to enter S phase and divide, resulting in 59 cells per posterior compartment on average as opposed to the wild-type number of 44. These cells are of a smaller size than wild-type cells. However, there is also an increase in the number of posterior compartment cells undergoing apoptosis and most cell death occurs at the front of the posterior compartment. At the larval stage, posterior compartment size is similar to wild type.

BrdU incorporation shows that the G1-phase of pIIIb cells expressing CycEScer\UAS.cLa under the control of Scer\GAL4neur-P72 is shorter than in wild-type cells. However, no BrdU incorporation is observed in the sibling glial cells. At 20 hours APF, the shaft and socket cells of Scer\GAL4neur-P72>CycEScer\UAS.cLa animals incorporate BrdU, while wild-type shaft and socket cells show no such incorporation.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4GMR.PF produces excess mitoses in the eye disc.

Expression of CycEScer\UAS.cLa under the regulation of Scer\GAL4sca-537.4 in the neuroectoderm results in an increase in the number of cells in the NB6-4a lineage (up to five cells) at approximately stages 11-14. Some of these cells migrate medially, as NB6-4 glial cells normally do (and express glial-specific markers), while others stay in a dorso-lateral position and express neuronal markers, suggesting a neuronal identity. In addition, overexpression results in transformation of NB6-4a to NB6-4t in 40% of embryos. Expression of CycEScer\UAS.cLa under the regulation of Scer\GAL4sca-537.4 in the neuroectoderm results in an increase in the number of cells in the NB6-4a lineage (up to five cells) at approximately stages 11-14. Some of these cells migrate medially and express glial-specific markers, as NB6-4a cells normally do, while others stay in a dorso-lateral position and express neuronal markers. In addition, overexpression results in transformation of NB6-4a to NB6-4t in 40% of embryos. This phenotype is cell-autonomous: single NB6-4a cells tranplanted from CycEScer\UAS.cLa; Scer\GAL4sca-537.4 to wild type embryos still make neurons as well as glial cells.

The salivary glands of CycEScer\UAS.cLa; Scer\GAL4ptc-559.1 larvae are small and their cells do not have endoreplicated DNA.

Ovarian follicle cells in somatic clones of CycEScer\UAS.cLa; Scer\GAL4Act5C.PP after stage 6 of oogenesis have small nuclei and do not incorporate BrdU indicating a lack of S phases.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4ptc-559.1 in the wing results in a slight increase in cell number.

When CycEScer\UAS.cLa is driven by Scer\GAL4bi-omb-Gal4 glial cells in the eye disc undergo excessive proliferation. However these glial cells never cross the axonal boundary.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4ey.PH has no effect on eye size.

When CycEScer\UAS.cLa is driven by Scer\GAL4GMR.PF, and the cells of the eye disc analysed using FACS analysis, a slight decrease in cell size is seen.

When expression is driven by Scer\GAL4en-e16E, no phenotype is detectable in the wing.

Almost no cells are in G1 in wing discs expressing CycEScer\UAS.cLa under the control of Scer\GAL4en-e16E, in contrast to wild-type wing discs. The number of posterior cells and the size of the posterior compartment in these wing discs is not significantly different from wild-type. The average duration of cell cycle phases in wing disc cells expressing CycEScer\UAS.cLa under the control of Scer\GAL4Act5C.PP is: G1 = 0.6 hours, S = 6.8 hours and G2 = 3.8 hours (wild-type values are G1 = 3.4 hours, S = 4.4 hours and G2 = 4.2 hours).

Scer\GAL4F4-mediated expression causes small nuclei in the third instar salivary glands: inhibition of endoreplication and growth, preceded initially by an ectopic S phase occurring just after the onset of ectopic CycE expression. Expression has no effect on adult viability.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference

CycEUAS.cLa, Scer\GAL4GMR.PF is a suppressor | partially of eye | temperature conditional phenotype of PEKUAS.cMa, Scer\GAL4GMR.PF

CycEUAS.cLa, Scer\GAL469B is a suppressor of wing & trichome phenotype of tumΔE1E.UAS, Scer\GAL469B

NOT Suppressor of
Statement
Reference

Scer\GAL4ey.PH/CycEUAS.cLa is a non-suppressor of eye phenotype of Lfee

Other
Additional Comments
Genetic Interactions
Statement
Reference

The expression of CycEScer\UAS.cLa under the control of Scer\GAL4elav-C155 suppresses the decreased neuroblast/neuroblast daughter divisions observed in wor4/Df(2L)ED1054 transheterozygous stage 14 embryos.

Co-expression of CycEScer\UAS.cLa fails to suppress the decreased nucleolar size phenotype seen in secondary neuroblasts in larval brains expressing bansponge.Scer\UAS.T:Disc\RFP under the control of Scer\GAL4insc-Mz1407.

The co-expression of CycEScer\UAS.cLa does not change the number of neuroblasts per brain lobe seen in larval brains expressing both bansponge.Scer\UAS.T:Disc\RFP and NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Expression of CycEScer\UAS.cLa suppresses the cell proliferation defects seen in the wing disc when caupScer\UAS.T:Ivir\HA1 is expressed under the control of either Scer\GAL4nub.PU or Scer\GAL4ap-md544 (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80ts.αTub84B).

Expression of CycEScer\UAS.cLa suppresses the cell proliferation defects seen in the wing disc when mirrScer\UAS.cMa is expressed under the control of Scer\GAL4nub.PU (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80ts.αTub84B).

Expression of CycEScer\UAS.cLa suppresses the cell proliferation defects seen in the wing disc when araScer\UAS.cGa is expressed under the control of Scer\GAL4nub.PU (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80ts.αTub84B).

Expression of caupScer\UAS.T:Ivir\HA1 suppresses the eye disc overgrowth seen when ykiScer\UAS.cUa is expressed under the control of Scer\GAL4salm.EPv. This suppression is partially reverted upon co-expression of CycEScer\UAS.cLa.

Expression of CycEScer\UAS.cLa suppresses the wing phenotypes seen when caupScer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4salm.EPv.

Expression of CycEScer\UAS.cLa suppresses the wing loss and formation of ectopic notum tissue seen when araScer\UAS.cGa is expressed in the prospective wing pouch under the control of Scer\GAL4MD-638.

Co-expression of CycEScer\UAS.cLa almost completely suppresses the wing hair spacing phenotype seen when Skp2KK108837 is expressed under the control of Scer\GAL4en-e16E.

The strong rough eye phenotype seen in flies expressing multiple copies of E2fdsRNA.Scer\UAS.cJa under the control of Scer\GAL4GMR.PU is partially suppressed by co-expression of CycEScer\UAS.cLa.

Late third instar wing discs expressing CycEScer\UAS.cLa and stgScer\UAS.cNa under the control of Scer\GAL4sd-SG29.1 have disturbed dorsal-ventral (DV) boundaries compared to controls.

The small eye phenotype caused by expression of kibraScer\UAS.cBa under the control of Scer\GAL4ey.PH is partially rescued by co-expression of either thEP3723 or CycEScer\UAS.cLa. Simultaneous co-expression of both thEP3723 and CycEScer\UAS.cLa almost completely rescues the small eye phenotype caused by expression of kibraScer\UAS.cBa under the control of Scer\GAL4ey.PH.

Expression of stgScer\UAS.cNa and CycEScer\UAS.cLa (using the MARCM system under the control of Scer\GAL4tub.PU) fails to rescue the growth defects seen in Df(1)btd-Sp1 leg disc clones generated in second instar larvae.

Co-expression of CycEScer\UAS.cLa with Scer\GAL4en-e16E>Dcr-1GD11429 restores cell and wing size.

The small, depigmented-eye phenotype of flies expressing PEKScer\UAS.cMa under the control of Scer\GAL4GMR.PF is partially suppressed by co-expression of CycEScer\UAS.cLa.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4ey.PH in a Lfee mutant background does not restore eye size.

Co-expression of geminindsRNA.Scer\UAS with CycEScer\UAS.cLa in 'flip out' clones in salivary glands does not rescue the block to DNA replication associated with the expression of CycEScer\UAS.cLa alone.

Expressing CycEScer\UAS.cLa under the control of Scer\GAL4en-e16E in Df(3L)XR38 embryos reduces the level of apoptosis in embryonic posterior compartment cells by 59% compared to when CycEScer\UAS.cLa is expressed in a wild-type background. Likewise, Scer\GAL4en-e16E>CycEScer\UAS.cLa expression in a W05014 background reduces apoptosis in these cells by 65%.

Coexpression of Egfr::toract.Scer\UAS and CycEScer\UAS.cLa, under the control of Scer\GAL4en-e16E, blocks CycEScer\UAS.cLa-induced apoptosis in the embryonic posterior compartment cells. There is a reduction in compartment size, similar to that seen when only Egfr::toract.Scer\UAS is expressed, and cells are of a size similar to that seen when only CycEScer\UAS.cLa is expressed. The same phenotype occurs when Ras85DV12.Scer\UAS and CycEScer\UAS.cLa are coexpressed.

Embryos that coexpress EgfrDN.Scer\UAS and CycEScer\UAS.cLa, under the control of Scer\GAL4en-e16E, show approximately double the amount of apoptosis in cells of posterior compartments relative to expressing CycEScer\UAS.cLa alone.

Expression of CycEScer\UAS.cLa, under the control of Scer\GAL4en-e16E, in spi1 embryos increases cell death in the posterior compartment, relative to expression of CycEScer\UAS.cLa in a wild-type background.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4prd.RG1 suppresses the mitotic phenotype of CycAC8LR1 embryos so that cells progress through mitosis 16 and a subsequent S phase.

Coexpression of either CycEScer\UAS.cLa with rappsm.Scer\UAS, under the control of Scer\GAL4prd.RG1, suppresses the inhibition of embryonic mitosis 16 seen when rappsm.Scer\UAS is expressed alone.

Expression of CycEScer\UAS.cLa under the control of Scer\GAL4neur-P72, in a dap4/+ background, induces an S-phase in 35% of sibling glial cells and 100% of pIIIb cells.

Co-expression of DlScer\UAS.cJa does not significantly modify the excess mitoses phenotype caused by Scer\GAL4GMR.PF-driven expression of CycEScer\UAS.cLa.

dmScer\UAS.cZa does not rescue salivary gland size in CycEScer\UAS.cLa; Scer\GAL4ptc-559.1 larvae, but does rescue endoreplication in this tissue to a limited degree.

Co-expression of Cdk2Scer\UAS.T:Hsap\MYC with CycEScer\UAS.cLa driven by Scer\GAL4GMR.PF blocks some photoreceptor differentiation in the third instar eye disc. The phenotype of these discs is largely unaffected by upd1Scer\UAS.cCa.

When CycEScer\UAS.cLa is expressed under the control of either Scer\GAL4ey.PB or Scer\GAL4ey.PH in NDN.Scer\UAS eye discs, rescue of the NDN.Scer\UAS mitotic phenotype is seen; eye discs are larger and contain more proliferating cells.

Coexpression of dltdsRNA.Scer\UAS and CycEScer\UAS.cLa, both under the control of Scer\GAL4ptc-559.1, results in a dramatic loss of wing cells.

CycEScer\UAS.cLa; Scer\GAL4prd.RG1 suppresses the mitotic block due to Rca12/Rca13, in alternate segments.

When CycEScer\UAS.cLa (driven by Scer\GAL4mf9) is added to Df(2L)Prl/prd4, (partially rescued by a weakly expressing prdmf9 line), the accessory gland phenotype is reduced, and male fertility is restored. When CycEScer\UAS.cLa (driven by Scer\GAL4prd.3.1) is added to Df(2L)Prl/prd4, however, no rescue is seen.

When CycEScer\UAS.cLa is driven by Scer\GAL4bi-omb-Gal4, in a eyaunspecified background, glial cells in the optic stalk undergo excessive proliferation. However these glial cells never enter the eye disc.

Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the eye under the control of Scer\GAL4ey.PH results in a much smaller eye than normal. Coexpression of CycEScer\UAS.cLa fully suppresses the phenotype.

stgScer\UAS.cNa and CycEScer\UAS.cLa bypass the effects of RbfScer\UAS.cDa on cell cycle phasing, cell numbers and cell size when they are both co-expressed with RbfScer\UAS.cDa using Scer\GAL4en-e16E.

Embryos expressing CycEScer\UAS.cLa or both CycEScer\UAS.cLa and rapScer\UAS.cSa under the control of Scer\GAL4prd.RG1 show normal development until and including mitosis 16 followed by an extra division cycle.

Xenogenetic Interactions
Statement
Reference

Expression of CycEScer\UAS.cLa partially suppresses the growth phenotype seen in the wings of flies expressing Hsap\TGIF2LXScer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E.

Co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa substantially suppresses the Scer\GAL4GMR.PF>Hsap\MAPTS396A.S404A.Scer\UAS-induced eye phenotype.

Co-expression of CycEScer\UAS.cLa strongly enhances the toxicity of Scer\GAL4GMR.PF>Hsap\MAPTT231A.S235A.Scer\UAS.

Co-expression of CycEScer\UAS.cLa strongly enhances the toxicity of Scer\GAL4GMR.PF>Hsap\MAPTS202A.T205A.Scer\UAS.

Co-expression of CycEScer\UAS.cLa strongly enhances the toxicity of Scer\GAL4GMR.PF>Hsap\MAPTScer\UAS.cWa.

Coexpression of CycEScer\UAS.cLa and BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, in the posterior compartment cells of embryos, blocks the apoptosis of these cells that occurs when CycEScer\UAS.cLa is expressed alone. The posterior compartments of these embryos show a further increase in the number of cells per posterior compartment compared to those expressing only CycEScer\UAS.cLa (77 vs 59). The Scer\GAL4en-e16E>CycEScer\UAS.cLa, BacA\p35Scer\UAS.cHa posterior compartments do not overgrow due to an extreme reduction in average cell size.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Overexpression of CycEScer\UAS.cLa using Scer\GAL4c323 or Scer\GAL4hs.PB does not result in incorporation of BrdU into chorion foci in stage 10A egg chambers, but does result in robust late replication of heterochromatin in stage 10A follicle cells as evidenced by increased BrdU labelling corresponding to the chromocenter.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
CycEScer\UAS.cLa
CycEUAS.cLa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Lane
Secondary FlyBase IDs
    References (57)