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General Information
Symbol
Dmel\rprUAS.C
Species
D. melanogaster
Name
FlyBase ID
FBal0059948
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-rpr, UAS-reaper, UASrpr
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

900bp EcoRI fragment containing a full-length rpr cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

lipid droplet & larval fat body, with Scer\GAL4sal.BO

Detailed Description
Statement
Reference

Expression of rprScer\UAS.C under the control of Scer\GAL4GMR.PFa results in rough eye phenotype and much reduced eye size in adult flies.

Expression of rprScer\UAS.C under the control of Scer\GAL4Desat1.PB results in larvae displaying tracheal flooding.

The clonal expression of rprScer\UAS.C under the control of Scer\GAL4Act5C.PI (together with Dicer-2, for efficient RNAi) within mosaic third instar larval wing discs leads to the basal delamination/extrusion in some expressing cells in both the wing pouch and hinge regions of the wing disc, as compared to controls.

Expression of rprScer\UAS.C using P{CoinFLP-GAL4} and Scer\FLP1ey.PN to stochastically generate patches of cells in the eye expressing Scer\GAL4Act5C.CoinFLP-FRT results in adult eyes which are normal in shape and size.

Expression of rprScer\UAS.C using P{AyGAL4} and Scer\FLP1ey.PN to generate clones in the eye expressing Scer\GAL4Act5C.PI results in ablation of the adult head.

Expression of rprScer\UAS.C under the control of Scer\GAL4sev.EP results in a rough eye phenotype with disruption of ommatidial arrangement.

Expression of rprScer\UAS.C under the control of Scer\GAL4Bx-MS1096 leads to a significant delay in pupariation, as compared to controls.

Expression of rprScer\UAS.C under the control of Scer\GAL4VT039046 results in a severe degradation of circadian locomotor activity rhythms under constant darkness conditions compared to controls: only approximately 2% of flies have strong rhythms and approximately 60% are completely arrhythmic.

Expression of rprScer\UAS.C under the control of Scer\GAL4SIFa.PT results in a degradation of circadian locomotor activity rhythms under constant darkness conditions compared to controls.

Flies expressing rprScer\UAS.C under the control of any of Scer\GAL4FMRFa.RS11, Scer\GAL4FMRFa.RS17 or Scer\GAL4FMRFa.PT are viable and develop normally. The majority of flies expressing rprScer\UAS.C under the control of Scer\GAL4FMRFa.RS8 die as pharate adults and ~20% of the resulting adults have distorted wings, move in an uncoordinated way and die within three days of eclosion.

The female flies expressing rprScer\UAS.C under the control of Scer\GAL4FMRFa.RS8 that have normal wings show reduced locomotor activity, which is only slightly elevated upon exposure to startling (air puffs). Climbing behavior not affected. Males show a similar activity and startle response to controls. When rprScer\UAS.C is expressed under the control of Scer\GAL4FMRFa.RS17 a modest decrease in startle-induced activity is seen, No defects are seen when rprScer\UAS.C is expressed under the control of either Scer\GAL4FMRFa.RS11 or Scer\GAL4FMRFa.PT.

Expression of rprScer\UAS.C under the control of Scer\GAL4GMR.PF causes 100% fly lethality at 24[o]C. When rprScer\UAS.C is expressed at 18[o]C 32.5% of pupae eclose. Eye size is reduced to ~50% compared to controls.

Expression of rprScer\UAS.C using Scer\GAL4Bx-MS1096 results in a delay in pupariation of 46.2 +/- 1.3 hours.

Targeted ablation of Ccap neurons through expression of rprScer\UAS.C under the control of Scer\GAL4Ccap.PP results in severe behavioral defects at pupal ecdysis. Although pre-ecdysis behavior appears normal and the duration of the period between the start of pre-ecdysis and anterior pullback is similar to that of controls, this anterior pullback is quite weak and is not followed by ecdysis behavior. Instead, it is followed by progressively weaker pre-ecdysis-like movements. As a result, most animals fail to properly evert their heads and extend their appendages, causing most to have reduced or non-existent heads and shorter than normal legs and wings.

Expression of rprScer\UAS.C in all circadian clock neurons (Scer\GAL4tim.PE) has no effect on light avoidance.

Expression of rprScer\UAS.C under the control of Scer\GAL4Gpb5 reduces fly viability.

Expression of rprScer\UAS.C under the control Scer\GAL4c42 results in first instar larval lethality.

Female flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB live significantly longer than controls, with an approximate 30% increase in median and 20% increase in maximum lifespan.

Scer\GAL4da.G32>rprScer\UAS.C females show reduced egg laying compared with controls.

Flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB all survive for significantly longer on food supplemented with 20mM paraquat compared to controls.

Flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB survive for longer in the presence of DTT, compared to controls.

Females expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB do not exhibit a delay in development but do display a significantly smaller body size compared to controls.

Expression of rprScer\UAS.C under the control of Scer\GAL4Ilp2.215-3 results in flies that have ablated median neurosecretory cells. Female flies lacking these cells show a significantly reduced re-mating rate 24 hours after mating to wild-type flies, compared to flies that have intact median neurosecretory cells.

Expression of rprScer\UAS.C in VA2 muscle founder cells under the control of Scer\GAL4slou.S59 does not result in a change in the number of SBM nuclei.

The decline in lifespan found upon increasing food concentration is almost completely disrupted by median neurosecretory cell ablation through expression of rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR. The maximal lifespan of ablated flies at the peak of the dietary restriction response is higher than that of controls. These flies do not live longer than control flies under starvation conditions but do exhibit a significantly longer lifespan under low sugar conditions.

Median neurosecretory cell (mNSC) ablation through expression of rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR results in decrease egg laying compared to controls at various food concentrations. No significant differences in feeding behavior are observed between mNSC ablated flies and controls. rprScer\UAS.C-Scer\GAL4Ilp2.PR flies show in increase in total locomotor activity when on high-yeast food compared to controls (indicating that the mNSCs may normally act to suppress the negative effect of yeast on activity). mNSC-ablated flies show a significant decrease in night sleep when on a low-nutrient diet (while wild-type controls show no effect).

Expression of rprScer\UAS.C under the control of Scer\GAL4ey.PH results in the lack of eclosion of flies. When flies are removed from their pupal casings, ablation of cephalic structures is found.

Expression of rprScer\UAS.C under the control of Scer\GAL4msn.PL results in substantial lamellocyte cell death, but only a few small melanotic masses, as expected with normal clearance of cells undergoing apoptosis. Blood smears of third instar larvae demonstrate a significant reduction in circulating lamellocytes, with other hemocyte population unaffected.

Expression of rprScer\UAS.C under the control of Scer\GAL4He.PZ results in a reduction in hemocyte cells, however, some third instar larvae still display aggregating hemocytes. Melanotic masses are rarely seen.

Scer\GAL4HCH.Hand>rprScer\UAS.C induced cell death commences at about embryonic stages, leaving the lymph glands freely floating by the beginning of first larval instar stage.

Expression of rprScer\UAS.C under the control of Scer\GAL4HCH.Hand results in the complete ablation of the dorsal vessel. Compared with controls, these flies show reduced life span, with precipitous increase in death on average at about five days post-eclosion.

Expression of rprScer\UAS.C under the control of Scer\GAL4elav-C155 is not very effective in ablating cells. However, stage 17 embryos expressing Scer\GAL4elav-C155>rprScer\UAS.C show the absence of the salivary glands. However, the ventral ganglion remains intact through the end of the larval first instar stage.

Scer\GAL4Switch1.PC-driven expression of rprScer\UAS.C induces apoptosis of midgut peripheral cells by the mid L3 stage. Subsequently, by late L3, adult midgut progenitor cells differentiate into polyploid enterocyte-like cells.

Reduced avoidance of carbon dioxide is seen in female flies expressing rprScer\UAS.C under the control of the Scer\GAL4Gr63a.PF driver.

Expression of rprScer\UAS.C under the control of the Scer\GAL4Gr63a.PF driver leads to an increase in lifespan in female flies compared to controls.

Scer\GAL4vg.PM-mediated expression of rprScer\UAS.C ablates the alula and axillary cell of the adult wing.

Scer\GAL4da.G32-mediated expression of rprScer\UAS.C results in premature amnioserosa dissociation and strong dorsal closure defects.

Newly eclosed males (within 24 hours of eclosion) expressing rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR show a reduction in the number of stage S2b spermatocyte cysts in the testis compared to wild-type males.

The average diameter of stage S6 spermatocytes in males expressing rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR is smaller than that of control spermatocytes at this stage.

Overexpression of rprScer\UAS.C under the control of Scer\GAL4ap-md544 and Scer\GAL80ts.αTub84B engenders non-cell autonomous apoptosis in the ventral compartment of third instar larval imaginal discs at around 24 hours after induction of rprScer\UAS.C-expression.

Expression of rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB ablates approximately half the insulin-producing cells (IPCs) specifically at the postlarval stage (larval IPCs are not ablated).

Larval growth and viability is not affected by expression of rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB.

Expression of rprScer\UAS.C using Scer\GAL4Ilp2.PR decreases body size.

Expression of rprScer\UAS.C under the control of Scer\GAL4Gr5a.PU abolishes the sucrose response but not the water response in these flies.

The ablation of all TA- and OA-containing neurons expression of rprScer\UAS.C under the control of Scer\GAL4Tdc2.PC leads to a profound decrease in flight initiation, flight duration per stimulation, and extended flight. Moreover, these mutants resume flight less often after stimulation compared with control animals. However, these flies are still able to fly and wing-beat frequencies are as wild-type.

Animals expressing rprScer\UAS.C under the control of Scer\GAL4Ccap.PA have severe defects at both the larval and pupal ecdyses, rarely yielding viable adult flies.

Larvae expressing rprScer\UAS.C under the control of Scer\GAL4sal.BO lack oenocytes from the first instar onwards, grow less than control larvae after the first-to-second instar transition and die before reaching pupariation, showing polyphasic lethality. Most second instar larvae expressing rprScer\UAS.C under the control of Scer\GAL4sal.BO show aberrant feeding behaviour, dispersing away from a yeast food source (the larvae enter and exist the yeast source multiple times). These larvae also retain food in the gut.

Larvae expressing rprScer\UAS.C under the control of Scer\GAL4sal.BO have a a higher density of lipid droplets in the fat body after starvation compared to control larvae.

Animals expressing rprScer\UAS.C under the control of Scer\GAL4sal.BO together with Scer\GAL80ts.αTub84B at 25oC can develop until pupal stages, as approximately 50% of oenocytes do not show apoptosis in these animals (due to attenuation of Scer\GAL4 activity by Scer\GAL80). These animals fail to complete pupal development, with many failing to separate from the puparial case during eclosion.

Socially enriched flies expressing rprScer\UAS.C under the control of Scer\GAL4ple.PF do not show a change in sleep when exposed to a period of social isolation, in contrast to control socially enriched flies which show a decrease in sleep requirement after exposure to social isolation.

Expression of rprScer\UAS.C in the adult brain under the control of Scer\GAL4elav.Switch.PO induces cell death, cell cycle activation is not induced.

Expression of rprScer\UAS.C, under the control of Scer\GAL4unspecified results in a pupal mortality rate of 79%.

Expression of rprScer\UAS.C under the control of Scer\GAL4Ccap.PP results either in pupal lethality, or survival to adulthood with a failure of wing expansion. Almost all neurons that express rprScer\UAS.C under the control of Scer\GAL4Ccap.PP are ablated.

Expression of two copies of rprScer\UAS.C using the "Split Gal4 system" where Scer\GAL4DBD.elav.T:Zzzz\ZipRREEL and Scer\GAL4AD.Ccap.T:Zzzz\ZipEERRL,T:SV40\nls2 together function as a GAL4 driver, results in wing expansion defects in over 90% of progeny with very few progeny showing pupal lethality. Approximately two thirds of Scer\GAL4DBD.elav.T:Zzzz\ZipRREEL,Scer\GAL4AD.Ccap.T:Zzzz\ZipEERRL,T:SV40\nls2>rprScer\UAS.C-expressing neurons are ablated. This phenotype is not seen if only one copy of rprScer\UAS.C is expressed.

Expression of one copy of the rprScer\UAS.C transgene, under the control of the "Split Gal4" driver that is generated by coexpressing Scer\GAL4DBD.Ccap.T:Zzzz\ZipRREEL and Hsim\VP16AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2, results in nearly complete pupal lethality and the death of almost all Ccap-expressing neurons.

Expression of rprScer\UAS.C under the control of the "Split Gal4" driver which results from expression of both Scer\GAL4DBD.Ccap.T:Zzzz\ZipRREEL and Hsim\VP16N4 hemidrivers causes ablation of the subesophageal and thoracic ganglia and six abdominal ganglion neurons that appear during pupal development and which do not express burs. This ablation is not fully penetrant and about 15% of animals of this genotype expand their wings normally and may therefore represent those that suffered little neuronal death.

Expression of rprScer\UAS.C under the control of the "Split Gal4" driver generated when Scer\GAL4DBD.Ccap.T:Zzzz\ZipRREEL and Hsim\VP16N6 are combined causes ablation of up to nine of the burs-expressing neurons but does not affect wing expansion.

When rprScer\UAS.C is driven by Scer\GAL4Ilp2.215-3 the median neurosecretory cells are ablated. These adults are only slightly smaller than controls and show no developmental delay. These flies exhibit a longer lifespan and reduced fecundity. The median lifespan is increased by 10.5% in males and 18.5% and 33% in virgin and mated females respectively. Aging related mortality starts later in mutants but proceeds at the same rate as controls. Decreases are seen in age specific egg laying for virgin and mated females. Mutant animals also have altered carbohydrate and lipid metabolism. Mutants contain levels of trehalose 64% higher than wildtype; glycogen is 44% higher and lipids 10% higher. Mutant animals also survive longer than controls when fed paraquat in their food, exhibit greater sensitivity to heat and cold shock, and are moderately starvation resistant.

Females in which insulin-like peptide (ILP)-producing brain cells have been ablated (by expression of rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR) have a severely impaired ability to upregulate follicle cell proliferation in response to a protein-rich diet. The rate of germline development is also reduced, in coordination with follicle cell divisions, because no abnormalities are seen in previtellogenic egg chambers.

rprScer\UAS.C overexpression in Gr5a-expressing cells under the control of Scer\GAL4Gr5a.853 results in a marked decrease in trehalose consumption and an increase in sucrose consumption, leading to similar consumption of both sugars.

Flies expressing rprScer\UAS.C under the control of Scer\GAL4Gr5a.853 exhibit a decrease in trehalose consumption at a variety of trehalose concentrations.

The lch5 chordotonal organs of stage 16 rprScer\UAS.C; Scer\GAL4repo embryos lack not only the repo expressing scolopidial ligament cells, but also the non-repo expressing ligament attachment cells. The resulting lch5 chordotonal organs are not fully stretched and have shorter than normal cap cells.

When rprScer\UAS.C is driven by Scer\GAL4Akh.PG, the corporia cardiaca (CC) cells are ablated. When these mutant larvae are grown on dextrose supplemented medium the mean total haemolymph glucose is reduced. The presence of rprScer\UAS.C and Scer\GAL4Akh.PG in larvae blocks the hyperglycaemic effect of tolbutamide.

Expression of rprScer\UAS.C, under the control of Scer\GAL4Ilp2.PR, leads to a slightly elevated risk of stress-induced cardiac failure for one-week-old flies, compared to wild-type flies of the same age. However, five-week-old flies expressing rprScer\UAS.C, under the control of Scer\GAL4Ilp2.PR, have a significantly reduced risk of cardiac failure compared to wild-type flies of the same age. Flies expressing rprScer\UAS.C, driven by Scer\GAL4Ilp2.PR, are smaller as adults and their development is delayed. Although their median survival rate is not significantly different from wild-type flies, a higher proportion of these flies are still alive at 80 days, while a lower proportion survive to 20-40 days, compared to wild-type flies.

When rprScer\UAS.C is driven by Scer\GAL4Appl.G1a or Scer\GAL4179Y, cell death is seen in neurons but no axon transport defects are seen.

Animals expressing rprScer\UAS.C under the control of Scer\GAL4puc-GAL4E69 die at an early larval stage.

When rprScer\UAS.C is driven by Scer\GAL4Ilp2.215-3, flies are viable but eclose one day later than control flies. Freshly eclosed flies show a slight but significant reduction in body weight and also reduced wing area. The change in size of the female abdomen is the most marked. This difference becomes further enhanced during the first three days of adult life. During this phase, egg production is stimulated by feeding and mating in wild-type females. There is a striking difference in ovary size in mutants: mutant females possess at most a single vitellogenic oocyte, unlike the multiple ones seen in wild-type. This phenotype leads to a reduced fecundity in mutant females, laying only 109 eggs per day (compared to about 60 in wild-type).

Larvae expressing rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR are only 58% of normal size at 120 hours of development. The developmental time to reach wandering third instar and puparium formation is 12 days in these larvae, compared to approximately 5 days in wild type. The adults produced have smaller wings than normal with both cell number and cell size being reduced.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4GMR.PF, rprUAS.C has lethal | heat sensitive phenotype, suppressible by BacA\p35UASp.cBa, Scer\GAL4GMR.PF

Scer\GAL4Ilp3.PB, rprUAS.C has long lived | drug conditional phenotype, suppressible | partially by foxoΔ94

NOT suppressed by
NOT Suppressor of
Statement
Reference

rprUAS.C/Scer\GAL4Ilp3.PB is a non-suppressor of viable phenotype of foxoΔ94

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4GMR.PFa, rprUAS.C has eye phenotype, enhanceable by CG8830[+]/DUBAIKG07439

NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The small rough eyes characteristic for flies expressing rprScer\UAS.C under the control of Scer\GAL4GMR.PFa cannot be rescued by co-expression of Usp15-31KK101035 and the phenotype is only partially suppressed (the size is rescued but not the rough appearance) by co-expression of DarkKK104215 or DroncdsRNA.UAS.cUa, whereas when the DarkKK104215 expression is combined with Usp15-31KK101035, it leads to a complete suppression. Combination of DroncdsRNA.UAS.cUa with Usp15-31KK101035 on the other hand does not strengthen the rescue and neither does the combination of DarkKK104215 with another transgene, Usp8KK100733. Co-expression of Usp8KK100733 alone has no effect on the rprScer\UAS.C-induced eye phenotype.

Lgr3ag1/Lgr3ag1 partially suppresses the delay in pupariation seen in larvae expressing rprScer\UAS.C under the control of Scer\GAL4Bx-MS1096.

When embryonic Ilp7 neurons are killed using WScer\UAS.cUa and rprScer\UAS.C (post-embryonic Ilp7 neurons are retained by limiting expression to the first and second instar larval stages using Scer\GAL80ts.αTub84B) female egg laying is normal.

When males express WScer\UAS.cUa and rprScer\UAS.C under the control of Scer\GAL4Ilp7.PC female egg laying is reduced on the first day after eclosion compared to wild type males, but subsequent egg laying is normal.

Females expressing WScer\UAS.cUa and rprScer\UAS.C throughout development under the control of Scer\GAL4Ilp7.PC have severely reduced egg laying. These females also have distended abdomens and eggs are always found jammed in the lateral oviduct. Of the small number of egg laid by these females, only 40% produce viable larvae.

The 46.2 +/- 1.3 hours delay in pupariation in rprScer\UAS.C, Scer\GAL4Bx-MS1096 animals is reverted to a delay of 27.8 +/- 2.9 hours in a Ilp8MI00727 background, and 29.1 +/-2.5 hours by co-expression of Ilp8KK112161.

Expression of both WScer\UAS.cUa and rprScer\UAS.C under the control of Scer\GAL4Rh5.PT abolishes light avoidance at the larval stage. However, expression under the control of Scer\GAL4Rh6.PD does not affect light avoidance.

Expression of both WScer\UAS.cUa and rprScer\UAS.C efficiently ablates all circadian clock neurons (Scer\GAL4tim.PE), leaving larvae with a seriously disrupted light avoidance phenotype. This phenotype is no different in LD or DD conditions.

Expression of both WScer\UAS.cUa and rprScer\UAS.C efficiently ablates the lateral neurons (Scer\GAL4P2.4.Pdf) but has no effect on light avoidance.

Late ablation of the mNSCs through expression of rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB does not affect the viability of foxoΔ94 flies.

A foxoΔ94 background suppresses the lifespan of female flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB, almost to wild-type levels.

foxoΔ94 mutant females expressing rprScer\UAS.C under the control of Scer\GAL4da.G32 show reduced egg laying compared with controls.

In a foxoΔ94 mutant background, Scer\GAL4Ilp3.PB driven expression of rprScer\UAS.C leads to significantly fewer eggs being laid than in controls.

foxoΔ94 mutant flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).

A foxoΔ94 background suppresses the DTT resistance seen in flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB.

A foxoΔ94 background does not affect the small body size seen upon expression of rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB.

Female flies expressing rprScer\UAS.C under the control of Scer\GAL4Ilp2.215-3 do not show a significantly reduced re-mating rate 5 hours after mating to Acp70A0 flies.

Males co-expressing WScer\UAS.cZa and rprScer\UAS.C under the control of Scer\GAL4CheB42a.ppk25 show courtship latency (time from female introduction until the male shows any courtship related behaviours) similar to that of parental controls.

Males co-expressing WScer\UAS.cZa and rprScer\UAS.C under the control of Scer\GAL4CheB42a.ppk25 show a mild change in courtship index (the proportion of time a male spends courting once courting started) which is not statistically significant compared to controls.

Co-expression of P{Sym-UAS-Hsrω} restores wing morphology to Scer\GAL4vg.PM, rprScer\UAS.C wings such that they are indistinguishable from wild type.

Co-expression of WScer\UAS.cHa and rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB ablates all of the insulin-producing cells (IPCs) specifically at the postlarval stage (larval IPCs are not ablated).

Flies co-expressing WScer\UAS.cHa and rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB and raised on fly maintenance food show no change in longevity compared to wild-type controls. Flies co-expressing WScer\UAS.cHa and rprScer\UAS.C under the control of Scer\GAL4Ilp3.PB and raised on fly food with yeast paste show an increase in life span compared to wild-type controls.

Females in which insulin-like peptide (ILP)-producing brain cells have been ablated (by expression of rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR) and which also express Ilp2hs.PR during larval development (to rescue developmental defects caused by the ablation of the ILP-producing brain cells) still show a reduced follicle cell proliferation rate in response to a protein-rich diet.

The combination of Khc9 and rprScer\UAS.C does not lead to any defect in axon transport.

The size of larvae expressing rprScer\UAS.C under the control of Scer\GAL4Ilp2.PR at 120 hours of development is rescued to 88% of normal by expression of Ilp2hs.PR. The wing growth defects are also rescued. The developmental time to reach wandering third instar and puparium formation is approximately 6 days in these rescued larvae, compared to 5 days in wild type.

Xenogenetic Interactions
Statement
Reference

Simultaneous expression of rprScer\UAS.C and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act.PU in somatic (flip-out) clones in third instar larval wing discs does not cause tumorigenic overgrowth although some ectopic tissue growth is observed.

Expression of BacA\p35Scer\UAS.P\T.cBa completely suppresses the lethality seen when rprScer\UAS.C is expressed under the control of Scer\GAL4GMR.PF at 24[o]C.

Expression of Zzzz\E4orf4Scer\UAS.cPa partially suppresses the semi-lethality seen when rprScer\UAS.C is expressed under the control of Scer\GAL4GMR.PF at 18[o]C. 78% of pupae eclose (compared to 32.5%). The reduction in eye size is also partially suppressed.

Complementation and Rescue Data
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Mutant
Wild-type
Stocks (6)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
rprScer\UAS.C
rprUAS.C
Name Synonyms
Secondary FlyBase IDs
    References (95)