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General Information
Symbol
Dmel\pydC5
Species
D. melanogaster
Name
FlyBase ID
FBal0059957
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P{lacZ-un1}pydC5
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
The P{lacZ-un1} insertion site is 7 bp upstream of the pyd transcription start.
Molecular lesion maps to the transcription unit previously described as 'tamou'.
Insertion of a P-element.
Insertion components
P{lacZ.ry+}pydC5
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
70% of pydtam/pydC5 flies display more than four dorsocentral macrochaetae.
No significant bristle or eye phenotypes are observed in pydC5 homozygotes.
pydC5 embryos display defects in tracheal development including dorsal branch fusion problems, ectopic terminal cells, a lack of fusion cells, and ganglionic branch outgrowth defects. The dorsal branch phenotype is not fully penetrant; 14% of embryos show wild-type tracheal systems, 22% show defects in 1 metamere, 25% show defects in 2-4 metameres and 39% show defects in >5 metameres. Fusion defects are occasionally observed in the lateral trunk, although the penetrance of this is lower than for the dorsal branch. pydC5/Df(3R)p-XT103 transheterozygotes show the same tracheal phenotypes as pydC5 homozygotes. pydC5 mutant clones which affect the terminal cells do not affect branch fusion to a significant extent, whereas pydC5 clones that affect the fusion cells are associated with branch fusion defects and rarely show a wild-type branch fusion pattern. Dorsal branches with pydC5 clones show large loops of intercellular adherens junctions, indicating that cell intercalation is not complete. These intercalation defects are not fully penetrant. In most cases, dorsal branches with a cell intercalation phenotype are still able to stretch and eventually reach the dorsal midline, and some can even undergo branch fusion.
pydC5/Df(3R)p819 embryos have a dorsal open phenotype.
Homozygous lethal and lethal in trans with Df(3R)p819 or Df(3R)pydC2. pyd1/pydC5, pydJ17/pydC5, pydJ4/pydC5, pydJ2/pydC5, pydG18/pydC5, pydG7/pydC5 and pydJ14/pydC5 flies have extra macrochaetae on the head and notum compared to wild-type.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
100% of N55e11/+; pydtam/pydC5 flies display more than four dorsocentral macrochaetae.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is reduced when pydScer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4. The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is reduced when pydΔPDZ1.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4. The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pydΔPDZ2.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4. The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pydΔPDZ3.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4. The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pydΔGUK.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4. The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pydΔEx6.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4. A more severe bristle phenotype is observed in these animals. The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is partially reduced when pydΔSH3.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4. The number of pydtam/pydC5 flies displaying an increased number of dorsocentral macrochaete is partially reduced when pydΔPro-Rich.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4sca-537.4.
Expression of either pydScer\UAS.T:Avic\GFP-EGFP or pydScer\UAS.cJa under the control of Scer\GAL4btl.PS partially rescues the tracheal phenotype of pydC5 embryos.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Order of alleles based on increasing severity of phenotype when hemizygous: pyd1 < pydJ17 < pydJ4 = pydJ2 < pydG18 = pydG7 < pydJ14 = pydC5. The extra bristle phenotype and lethality of pydC5 revert simultaneously in the presence of a source of P-element transposase, suggesting that both phenotypes are due to insertion of the P-element. However, a lethal mutation that fails to complement the lethality of pydC5, but complements the bristle phenotype of pydJ14 has been isolated, suggesting that the P-element insertion in pydC5 may affect both pyd and an independent locus.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)