Allele Dmel\pb^Scer\UAS.A

General Information
Symbol	Dmel\pb^Scer\UAS.A	Species	D. melanogaster
Name		FlyBase ID	FBal0059978
Feature type	allele	Associated gene	Dmel\pb

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Aplin and Kaufman, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: 3.5kb SacII-KpnI fragment containing the entire pb cDNA is cloned into the pUAST vector. (Aplin and Kaufman, 1997)
Carried in construct	P{UAS-pb.A} (Abzhanov et al., 2001, Hirth et al., 2001, Benassayag et al., 2003, Adachi-Yamada et al., 2005, Joulia et al., 2005, Sprecher et al., 2004, Aplin and Kaufman, 1997, Yasunaga et al., 2006, Anderson et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^69B (Aplin and Kaufman, 1997) lethal, with Scer\GAL4^71B (Aplin and Kaufman, 1997) lethal \| pupal stage, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997)
Phenotype Manifest In
	antenna, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997) crossvein, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997) embryonic tritocerebrum, with Scer\GAL4^sca-537.4 (Sprecher et al., 2004) eye, with Scer\GAL4^dpp.blk1 (Benassayag et al., 2003) eye, with Scer\GAL4^ey.PH (Benassayag et al., 2003) imaginal disc, with Scer\GAL4^dpp.blk1 (Abzhanov et al., 2001) leg, with Scer\GAL4^30A (Aplin and Kaufman, 1997) leg, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997, Benassayag et al., 2003) macrochaeta, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997) tarsal segment, with Scer\GAL4^30A (Aplin and Kaufman, 1997) tarsal segment, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997) wing, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997, Benassayag et al., 2003) wing & macrochaeta, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997) wing margin, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005) wing vein, with Scer\GAL4^dpp.blk1 (Aplin and Kaufman, 1997) wing vein, with Scer\GAL4^sd-SG29.1 (Adachi-Yamada et al., 2005)
Detailed Description
	Statement Reference When pb^Scer\UAS.A is driven by Scer\GAL4^sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen. (Adachi-Yamada et al., 2005) Expression of pb^Scer\UAS.A under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%. (Sprecher et al., 2004) When pb^Scer\UAS.A is driven by Scer\GAL4^dpp.blk1 a substantial eye loss phenotype as well as wing and leg defects are seen. When pb^Scer\UAS.A is driven by Scer\GAL4^ey.PH a substantial or full eye loss phenotype is seen. When driven by Scer\GAL4^GMR.PF no effect is seen. (Benassayag et al., 2003) Most imaginal discs expressing pb^Scer\UAS.A under the control of Scer\GAL4^dpp.blk1 are misshapen; the discs have abnormal folding and do not lay flat like wild-type discs. (Abzhanov et al., 2001) Expression of pb^Scer\UAS.A under the control of Scer\GAL4^lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain. (Hirth et al., 2001) Scer\GAL4^dpp.blk1-mediated expression in the imaginal discs results in striking growth and patterning defects in the antenna, legs and wings. Upon eclosion flies are unable to pull themselves free of the pupal case. Labial-like structures are observed along the T1 legs, maxillary palp-like structures are formed along the lateral aspects of the legs in some locations. General leg patterning defects include a shortening and distortion of the leg along the PD axis, fusion of tarsal segments and large bulbous outgrowths of tissue along the lateral surfaces. Wings do not exhibit homeotic transformation but are smaller in size than wild type, are missing both longitudinal and cross veins, have ectopic socketed bristles growing from the wing blade surface and display a crumpled appearance. Ectopic expression does not increase apoptosis in the wing, leg, haltere or eye discs. Scer\GAL4^69B- or Scer\GAL4^71B-mediated expression causes embryonic or early larval lethality. Scer\GAL4^30A-mediated expression causes minor non-homeotic disruptions of the legs that include fusion of tarsal segments. (Aplin and Kaufman, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference pb^Scer\UAS.A/Scer\GAL4^lz-gal4 is a suppressor of visible phenotype of Scer\GAL4^lz-gal4, sens^Scer\UAS.cNa (Anderson et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference pb^Scer\UAS.A/Scer\GAL4^dpp.blk1 is a suppressor of eye \| ectopic phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Benassayag et al., 2003) pb^Scer\UAS.A/Scer\GAL4^lab.PH is a suppressor of embryonic tritocerebrum phenotype of lab¹⁴ (Hirth et al., 2001) pb^Scer\UAS.A/Scer\GAL4^lz-gal4 is a suppressor of eye phenotype of Scer\GAL4^lz-gal4, sens^Scer\UAS.cNa (Anderson et al., 2011) pb^Scer\UAS.A/Scer\GAL4^pb.PJ is a suppressor of pseudotracheal sensillum phenotype of Scer\GAL4^pb.PJ, ci^Scer\UAS.cAa (Joulia et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of pb[Scer\UAS.A] strongly suppresses the disorganised eye phenotype caused by expression of sens[Scer\UAS.cNa] under the control of Scer\GAL4[lz-gal4]. (Anderson et al., 2011) When pb^Scer\UAS.A is overexpressed along with ci^Scer\UAS.cAa, both under the control of Scer\GAL4^pb.PJ, the ci^Scer\UAS.cAa phenotype is suppressed and labial palps appear almost the same as wild type. (Joulia et al., 2005) Expression of pb^Scer\UAS.A under the control of Scer\GAL4^lab.PH rescues the tritocerebral defects seen in lab¹⁴ embryonic brains. 41.7% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab¹⁴ phenotype is 88.6%). (Hirth et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	7298 w¹¹¹⁸; P{UAS-pb.A}49.1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	pb^Scer\UAS.A pb^UAS.A
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Anderson et al., 2011, Development 138(10): 1957--1966 The enhancer of trithorax and polycomb gene Caf1/p55 is essential for cell survival and patterning in Drosophila development. [FBrf0213580] Yasunaga et al., 2006, Mech. Dev. 123(12): 893--906 Fate map of the distal portion of Drosophila proboscis as inferred from the expression and mutations of basic patterning genes. [FBrf0194566] Adachi-Yamada et al., 2005, Mol. Cell. Biol. 25(8): 3140--3150 Wing-to-leg homeosis by Spineless causes apoptosis regulated by Fish-lips, a novel leucine-rich repeat transmembrane protein. [FBrf0184157] Joulia et al., 2005, Dev. Biol. 278(2): 496--510 Homeotic proboscipedia function modulates hedgehog-mediated organizer activity to pattern adult Drosophila mouthparts. [FBrf0183854] Sprecher et al., 2004, Mech. Dev. 121(6): 527--536 Hox gene cross-regulatory interactions in the embryonic brain of Drosophila. [FBrf0179451] Benassayag et al., 2003, Development 130(3): 575--586 Evidence for a direct functional antagonism of the selector genes proboscipedia and eyeless in Drosophila head development. [FBrf0155717] Abzhanov et al., 2001, Development 128(14): 2803--2814 The Drosophila proboscis is specified by two Hox genes, proboscipedia and Sex combs reduced, via repression of leg and antennal appendage genes. [FBrf0138361] Hirth et al., 2001, Development 128(23): 4781--4788 Functional equivalence of Hox gene products in the specification of the tritocerebrum during embryonic brain development of Drosophila. [FBrf0141495] Aplin and Kaufman, 1997, Mech. Dev. 62(1): 51--60 Homeotic transformation of legs to mouthparts by proboscipedia expression in Drosophila imaginal discs. [FBrf0093060]

Allele Dmel\pbScer\UAS.A

Allele Dmel\pb^Scer\UAS.A