Allele Dmel\pan^Scer\UAS.cWa

General Information
Symbol	Dmel\pan^Scer\UAS.cWa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of van de Wetering	FlyBase ID	FBal0059980
Feature type	allele	Associated gene	Dmel\pan

Allele class
Mutagen	in vitro construct
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct (van de Wetering et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a fragment of full length pan is directed by Scer\UAS Scer\GAL4 binding sites. (van de Wetering et al., 1997)
Carried in construct	P{UAS-pan.dTCF} (Kiger et al., 2001, Jackson et al., 2002, Verheyen et al., 2001, Yang et al., 2000, Helms et al., 1999, Tolwinski and Wieschaus, 2001, Cavallo et al., 1998, Waltzer and Bienz, 1998, van de Wetering et al., 1997, Chao et al., 2007, Lawrence et al., 2000, Haussmann et al., 2008, Berger et al., 2005, Morris et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^elav-C155 (Jackson et al., 2002) lethal, with Scer\GAL4^hs.2sev (Jackson et al., 2002) neuroanatomy defective \| somatic clone \| third instar larval stage, with Scer\GAL4^elav.PH (Haussmann et al., 2008) partially lethal - majority die \| pupal stage, with Scer\GAL4^dpp.blk1 (Chao et al., 2007) visible, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) visible, with Scer\GAL4^sd-SG29.1 (Lawrence et al., 2000) visible, with Scer\GAL4^sev.EP (Jackson et al., 2002) visible, with Scer\GAL4^vg.int2.1 (Verheyen et al., 2001) wild-type, with Scer\GAL4^e22c (Cavallo et al., 1998)
Phenotype Manifest In
	denticle belt, with Scer\GAL4^e22c (van de Wetering et al., 1997) embryonic/larval neuromuscular junction \| somatic clone, with Scer\GAL4^elav.PH (Haussmann et al., 2008) interommatidial bristle, with Scer\GAL4^sev.EP (Jackson et al., 2002) NMJ bouton \| somatic clone, with Scer\GAL4^elav.PH (Haussmann et al., 2008) wing, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) wing, with Scer\GAL4^sd-SG29.1 (Lawrence et al., 2000) wing margin, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) wing margin, with Scer\GAL4^sd-SG29.1 (Lawrence et al., 2000) wing margin \| posterior, with Scer\GAL4^vg.int2.1 (Verheyen et al., 2001) wing sensillum \| ectopic, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) wing vein \| ectopic, with Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000)
Detailed Description
	Statement Reference Expression of pan[Scer\UAS.cWa] under the control of Scer\GAL4[elav.PH] in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4[elav.PH] driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type. (Haussmann et al., 2008) Expression of pan[Scer\UAS.cWa] under the control of Scer\GAL4[Act88F.PD] does not result in a mutant wing phenotype. (Morris et al., 2008) Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^arm.PS results in approximately 1% embryonic lethality. Expression under the control of Scer\GAL4^e22c results in approximately 1.8% embryonic lethality. Expression under the control of Scer\GAL4^dpp.blk1 results in approximately 36.7% pupal lethality. (Chao et al., 2007) When pan^Scer\UAS.cWa is driven by Scer\GAL4^sev.EP eyes appear to be normal other than a weak interommatidial bristle phenotype. (Jackson et al., 2002) Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^vg.int2.1 results in defects in the posterior wing margin. (Verheyen et al., 2001) Expression of pan[Scer\UAS.cWa] under the control of Scer\GAL4[sd-SG29.1] results in reduced wings with severe patterning defects including loss of wing margin. Expression of pan[Scer\UAS.cWa] under the control of Scer\GAL4[Bx-MS1096] results in a reduction in the size of the wing. Ectopic bristles and vein tissue are seen throughout the wing blade and the wing margin is consistently nicked. (Lawrence et al., 2000) Scer\GAL4^e22c-mediated expression causes full or partial embryonic viability or embryonic lethality. Viable lines show slight narrowing of the abdominal denticle belts. (van de Wetering et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference pan^Scer\UAS.cWa has lethal \| embryonic stage phenotype, enhanceable by Scer\GAL4^arm.PS/SoxN^Scer\UAS.cOa (Chao et al., 2007) pan^Scer\UAS.cWa has lethal \| embryonic stage phenotype, enhanceable by SoxN^Scer\UAS.cOa/Scer\GAL4^e22c (Chao et al., 2007) pan^Scer\UAS.cWa has lethal \| pupal stage phenotype, enhanceable by Scer\GAL4^dpp.blk1/SoxN^Scer\UAS.cOa (Chao et al., 2007) Scer\GAL4^sev.EP, pan^Scer\UAS.cWa has visible phenotype, enhanceable by Hsap\MAPT^GMR.Ex.PJ (Jackson et al., 2002)
Suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has visible phenotype, suppressible \| partially by arm^Scer\UAS.cSa/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000)
Enhancer of
Statement Reference pan^Scer\UAS.cWa/Scer\GAL4^e22c is an enhancer of lethal \| embryonic stage phenotype of SoxN^Scer\UAS.cOa (Chao et al., 2007) Scer\GAL4^arm.PS/pan^Scer\UAS.cWa is an enhancer of lethal \| embryonic stage phenotype of SoxN^Scer\UAS.cOa (Chao et al., 2007) Scer\GAL4^dpp.blk1/pan^Scer\UAS.cWa is an enhancer of lethal \| pupal stage phenotype of SoxN^Scer\UAS.cOa (Chao et al., 2007) Scer\GAL4^sev.EP/pan^Scer\UAS.cWa is an enhancer of visible phenotype of Hsap\MAPT^GMR.Ex.PJ (Jackson et al., 2002)
Suppressor of
Statement Reference pan^Scer\UAS.cWa, Scer\GAL4^Act88F.PD is a suppressor of visible phenotype of E2f^Scer\UAS.cNa, Scer\GAL4^Act88F.PD (Morris et al., 2008) Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2}, Scer\GAL4^ey.PB, pan^Scer\UAS.cWa is a suppressor \| partially of visible phenotype of Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2}/Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} (Berger et al., 2005)
Other
Statement Reference Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A, pan^Scer\UAS.cWa has lethal \| partially phenotype (Kiger et al., 2001, Kiger et al., 2001)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^sev.EP, pan^Scer\UAS.cWa has interommatidial bristle phenotype, enhanceable by Hsap\MAPT^GMR.Ex.PJ (Jackson et al., 2002)
Suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has wing margin phenotype, suppressible by gro^{Nterm.Scer\UAS}/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has wing phenotype, suppressible \| partially by arm^Scer\UAS.cSa/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has wing sensillum \| ectopic phenotype, suppressible \| partially by arm^Scer\UAS.cSa/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has wing vein \| ectopic phenotype, suppressible \| partially by arm^Scer\UAS.cSa/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000)
NOT suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has wing margin phenotype, non-suppressible by arm^Scer\UAS.cSa/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has wing sensillum \| ectopic phenotype, non-suppressible by gro^{Nterm.Scer\UAS}/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000) Scer\GAL4^Bx-MS1096, pan^Scer\UAS.cWa has wing vein \| ectopic phenotype, non-suppressible by gro^{Nterm.Scer\UAS}/Scer\GAL4^Bx-MS1096 (Lawrence et al., 2000)
Enhancer of
Statement Reference Scer\GAL4^arm.PS/pan^Scer\UAS.cWa is an enhancer of denticle belt phenotype of SoxN^Scer\UAS.cOa (Chao et al., 2007) Scer\GAL4^arm.PS/pan^Scer\UAS.cWa is an enhancer of embryonic/first instar larval cuticle phenotype of SoxN^Scer\UAS.cOa (Chao et al., 2007) Scer\GAL4^sev.EP/pan^Scer\UAS.cWa is an enhancer of eye \| anterior phenotype of Hsap\MAPT^GMR.Ex.PJ (Jackson et al., 2002) Scer\GAL4^sev.EP/pan^Scer\UAS.cWa is an enhancer of interommatidial bristle phenotype of Hsap\MAPT^GMR.Ex.PJ (Jackson et al., 2002) Scer\GAL4^sev.EP/pan^Scer\UAS.cWa is an enhancer of ommatidium phenotype of Hsap\MAPT^GMR.Ex.PJ (Jackson et al., 2002)
NOT Enhancer of
Statement Reference pan^Scer\UAS.cWa/Scer\GAL4^30A is a non-enhancer of wing phenotype of bae¹ (Kiger et al., 2001)
Suppressor of
Statement Reference pan^Scer\UAS.cWa, Scer\GAL4^30A is a suppressor of wing phenotype of Pka-C1^Scer\UAS.cKa, Scer\GAL4^30A (Kiger et al., 2001) pan^Scer\UAS.cWa, Scer\GAL4^Act88F.PD is a suppressor \| partially of wing phenotype of E2f^Scer\UAS.cNa, Scer\GAL4^Act88F.PD (Morris et al., 2008) Scer\GAL4^sev.EP/pan^Scer\UAS.cWa is a suppressor \| partially of rhabdomere \| conditional phenotype of Hsap\HTT^GMR.Q120 (Berger et al., 2005)
NOT Suppressor of
Statement Reference pan^Scer\UAS.cWa/Scer\GAL4^30A is a non-suppressor of wing phenotype of bae¹ (Kiger et al., 2001) pan^Scer\UAS.cWa/Scer\GAL4^69B is a non-suppressor of embryonic/first instar larval cuticle \| dorsal phenotype of hep¹ (Kaltschmidt et al., 2002) Scer\GAL4^bbg-C96/pan^Scer\UAS.cWa is a non-suppressor of wing phenotype of Scer\GAL4^bbg-C96, mam^N.Scer\UAS (Helms et al., 1999)
Other
Statement Reference Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^sev.EP, pan^Scer\UAS.cWa has eye \| anterior phenotype (Jackson et al., 2002, Jackson et al., 2002) Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^sev.EP, pan^Scer\UAS.cWa has ommatidium phenotype (Jackson et al., 2002, Jackson et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of pan[Scer\UAS.cWa] partially suppresses the gnarled wing phenotype caused by expression of E2f[Scer\UAS.cNa] under the control of Scer\GAL4[Act88F.PD]. (Morris et al., 2008) Expression of both SoxN^Scer\UAS.cOa and pan^Scer\UAS.cWa under the control of Scer\GAL4^arm.PS results in 87.6% embryonic lethality. Expression under the control of Scer\GAL4^e22c results in approximately 99.6% embryonic lethality, while expression of both SoxN^Scer\UAS.cOa and pan^Scer\UAS.cWa under the control of Scer\GAL4^dpp.blk1 results in approximately 100% pupal lethality. Overexpression of pan^Scer\UAS.cWa enhances the repressive capacity of overexpressed SoxN^Scer\UAS.cOa. This is particularly apparent when both transgenes are expressed under the control of Scer\GAL4^arm.PS. Under these conditions, the cuticle pattern is most disrupted, both ventrally and dorsally. Ectopic denticles appear with greater frequency and broader distribution across the ventral naked cuticle domain. (Chao et al., 2007) pan^Scer\UAS.cWa with Scer\GAL4^69B fails to significantly suppress the dorsal hole phenotype of hep¹ embryo cuticles. (Kaltschmidt et al., 2002) Co-expression of arm[Scer\UAS.cSa] partially suppresses the wing defects caused by expression of pan[Scer\UAS.cWa] under the control of Scer\GAL4[Bx-MS1096]: the reduction in wing size, ectopic wing bristle phenotype and wing venation patterns are partially rescued, but the nicking of the wing margin is not suppressed. Co-expression of arm[ΔC.Scer\UAS] and pan[Scer\UAS.cWa] under the control of Scer\GAL4[Bx-MS1096] results in a small wing with a highly disrupted venation pattern and many ectopic bristles. Co-expression of gro[Nterm.Scer\UAS] in animals expressing pan[Scer\UAS.cWa] under the control of Scer\GAL4[Bx-MS1096] results in recovery of wing margin close to the hinge, but the reduced size of the wing and ectopic bristle phenotypes are not rescued. (Lawrence et al., 2000) pan^Scer\UAS.cWa, Scer\GAL4^C96 flies show minor wing nicking. Does not suppress wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies. (Helms et al., 1999)
Xenogenetic Interactions
Statement Reference Expression of pan[Scer\UAS.cWa] under the control of Scer\GAL4[sev.EP] partially suppresses the loss of rhabdomeres seen in Hsap\HTT[GMR.Q120] flies. Co-expression of pan[Scer\UAS.cWa] decreases the fraction of flies with rough eyes in animals expressing Zzzz\Poly-Ala[A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2] under the control of Scer\GAL4[ey.PB]. (Berger et al., 2005) Hsap\MAPT^GMR.Ex.PJ and pan^Scer\UAS.cWa (driven by Scer\GAL4^hs.2sev) enhance each other leading to more roughened anterior part of the eye, with fusion, pitting, and collapse of ommatidia along with irregularity of bristles. (Jackson et al., 2002)
Complementation & Rescue Data
Comments
Stocks ( 4 )
Bloomington	4838 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}4 4837 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}24/CyO
Kyoto	107900 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}4 107899 y¹ w¹¹¹⁸; P{UAS-pan.dTCF}24/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	pan^Scer\UAS.cWa pan^UAS.cWa
Name Synonym	Saccharomyces cerevisiae UAS construct a of van de Wetering (van de Wetering et al., 1997)
Secondary FlyBase IDs

References ( 15 )
Research paper	Haussmann et al., 2008, Genome Biol. 9(4): R73 Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways. [FBrf0209726] Morris et al., 2008, Nature 455(7212): 552--556 E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. [FBrf0221005] Chao et al., 2007, Development 134(5): 989--997 The HMG-box transcription factor SoxNeuro acts with Tcf to control Wg/Wnt signaling activity. [FBrf0195251] Berger et al., 2005, Hum. Mol. Genet. 14(20): 3003--3011 Lithium rescues toxicity of aggregate-prone proteins in Drosophila by perturbing Wnt pathway. [FBrf0190825] Jackson et al., 2002, Neuron 34(4): 509--519 Human wild-type tau interacts with wingless pathway components and produces neurofibrillary pathology in Drosophila. [FBrf0149139] Kaltschmidt et al., 2002, Nat. Cell Biol. 4(12): 937--944 Planar polarity and actin dynamics in the epidermis of Drosophila. [FBrf0155993] Kiger et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(18): 10190--10195 Hemocytes are essential for wing maturation in Drosophila melanogaster. [FBrf0138518] Tolwinski and Wieschaus, 2001, Development 128(11): 2107--2117 Armadillo nuclear import is regulated by cytoplasmic anchor Axin and nuclear anchor dTCF/Pan. [FBrf0136754] Verheyen et al., 2001, Mech. Dev. 101(1-2): 119--132 The tissue polarity gene nemo carries out multiple roles in patterning during Drosophila development. [FBrf0134745] Lawrence et al., 2000, Int. J. Dev. Biol. 44(7): 749--756 dTcf antagonises Wingless signalling during the development and patterning of the wing in Drosophila. [FBrf0132357] Yang et al., 2000, Development 127(17): 3695--3702 decapentaplegic is a direct target of dTcf repression in the Drosophila visceral mesoderm. [FBrf0130179] Helms et al., 1999, Dev. Biol. 215(2): 358--374 Engineered truncations in the Drosophila mastermind protein disrupt Notch pathway function. [FBrf0111904] Cavallo et al., 1998, Nature 395(6702): 604--608 Drosophila Tcf and Groucho interact to repress Wingless signalling activity. [FBrf0104944] Waltzer and Bienz, 1998, Nature 395(6701): 521--525 Drosophila CBP represses the transcription factor TCF to antagonize Wingless signalling. [FBrf0104794] van de Wetering et al., 1997, Cell 88(6): 789--799 Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF. [FBrf0093264]

Allele Dmel\panScer\UAS.cWa

Allele Dmel\pan^Scer\UAS.cWa