FlyBase Allele Report: Dmel\pan[UAS.cWa]

General Information

Symbol

Dmel\pan^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of van de Wetering

FlyBase ID

FBal0059980

Feature type

allele

Associated gene

Dmel\pan

Associated Insertion(s)

Carried in Construct

P{UAS-pan.dTCF}

Also Known As

UAS-dTCF, UAS-pan, UASdTcf, UAS-Tcf

Key Links

Transgenic product class

wild_type

(van de Wetering et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(van de Wetering et al., 1997)

Mutagen

in vitro construct

(van de Wetering et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-pan.dTCF}

Cytology

Description

UASt regulatory sequences drive expression of full length pan.

(van de Wetering et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

pan

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone | third instar larval stage, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

lethal, with Scer\GAL4^elav-C155

(Jackson et al., 2002)

lethal, with Scer\GAL4^hs.2sev

(Jackson et al., 2002)

partially lethal - majority die, with Scer\GAL4^dpp.blk1

(Chao et al., 2007)

some die during pupal stage, with Scer\GAL4^dpp.blk1

(Chao et al., 2007)

visible, with Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

visible, with Scer\GAL4^sd-SG29.1

(Lawrence et al., 2000)

visible, with Scer\GAL4^sev.EP

(Jackson et al., 2002)

visible, with Scer\GAL4^vg.int2.1

(Verheyen et al., 2001)

wild-type, with Scer\GAL4^e22c

(Cavallo et al., 1998)

Phenotype Manifest In

embryonic/larval neuromuscular junction | somatic clone, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

interommatidial bristle, with Scer\GAL4^sev.EP

(Jackson et al., 2002)

NMJ bouton | somatic clone, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

ventral denticle belt, with Scer\GAL4^e22c

(van de Wetering et al., 1997)

wing, with Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

wing, with Scer\GAL4^sd-SG29.1

(Lawrence et al., 2000)

wing margin, with Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

wing margin, with Scer\GAL4^sd-SG29.1

(Lawrence et al., 2000)

wing margin | posterior, with Scer\GAL4^vg.int2.1

(Verheyen et al., 2001)

wing sensillum | ectopic, with Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Detailed Description

Statement

Reference

Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4^elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

(Haussmann et al., 2008)

Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^Act88F.PD does not result in a mutant wing phenotype.

(Morris et al., 2008)

Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^arm.PS results in approximately 1% embryonic lethality. Expression under the control of Scer\GAL4^e22c results in approximately 1.8% embryonic lethality. Expression under the control of Scer\GAL4^dpp.blk1 results in approximately 36.7% pupal lethality.

(Chao et al., 2007)

When pan^Scer\UAS.cWa is driven by Scer\GAL4^sev.EP eyes appear to be normal other than a weak interommatidial bristle phenotype.

(Jackson et al., 2002)

Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^vg.int2.1 results in defects in the posterior wing margin.

(Verheyen et al., 2001)

Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^sd-SG29.1 results in reduced wings with severe patterning defects including loss of wing margin.

Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 results in a reduction in the size of the wing. Ectopic bristles and vein tissue are seen throughout the wing blade and the wing margin is consistently nicked.

(Lawrence et al., 2000)

Scer\GAL4^e22c-mediated expression causes full or partial embryonic viability or embryonic lethality. Viable lines show slight narrowing of the abdominal denticle belts.

(van de Wetering et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pan^UAS.cWa has lethal | embryonic stage phenotype, enhanceable by Scer\GAL4^arm.PS/SoxN^UAS.cOa

(Chao et al., 2007)

pan^UAS.cWa has lethal | embryonic stage phenotype, enhanceable by SoxN^UAS.cOa/Scer\GAL4^e22c

(Chao et al., 2007)

pan^UAS.cWa has lethal | pupal stage phenotype, enhanceable by Scer\GAL4^dpp.blk1/SoxN^UAS.cOa

(Chao et al., 2007)

Scer\GAL4^sev.EP, pan^UAS.cWa has visible phenotype, enhanceable by Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has visible phenotype, suppressible | partially by arm^UAS.cSa/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Enhancer of

Statement

Reference

pan^UAS.cWa/Scer\GAL4^e22c is an enhancer of lethal | embryonic stage phenotype of SoxN^UAS.cOa

(Chao et al., 2007)

Scer\GAL4^dpp.blk1/pan^UAS.cWa is an enhancer of lethal | pupal stage phenotype of SoxN^UAS.cOa

(Chao et al., 2007)

Scer\GAL4^arm.PS/pan^UAS.cWa is an enhancer of lethal | embryonic stage phenotype of SoxN^UAS.cOa

(Chao et al., 2007)

Scer\GAL4^sev.EP/pan^UAS.cWa is an enhancer of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Suppressor of

Statement

Reference

pan^UAS.cWa, Scer\GAL4^Act88F.PD is a suppressor of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2008)

Scer\GAL4^ey.PB/pan^UAS.cWa is a suppressor | partially of visible phenotype of Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2005)

Other

Statement

Reference

Pka-C1^UAS.cKa, Scer\GAL4^30A, pan^UAS.cWa has partially lethal phenotype

(Kiger et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sev.EP, pan^UAS.cWa has interommatidial bristle phenotype, enhanceable by Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has wing margin phenotype, suppressible by gro^Nterm.UAS/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has wing phenotype, suppressible | partially by arm^UAS.cSa/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has wing vein | ectopic phenotype, suppressible | partially by arm^UAS.cSa/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has wing sensillum | ectopic phenotype, suppressible | partially by arm^UAS.cSa/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has wing margin phenotype, non-suppressible by arm^UAS.cSa/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has wing vein | ectopic phenotype, non-suppressible by gro^Nterm.UAS/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Scer\GAL4^Bx-MS1096, pan^UAS.cWa has wing sensillum | ectopic phenotype, non-suppressible by gro^Nterm.UAS/Scer\GAL4^Bx-MS1096

(Lawrence et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^arm.PS/pan^UAS.cWa is an enhancer of embryonic/first instar larval cuticle phenotype of SoxN^UAS.cOa

(Chao et al., 2007)

Scer\GAL4^arm.PS/pan^UAS.cWa is an enhancer of ventral denticle belt phenotype of SoxN^UAS.cOa

(Chao et al., 2007)

Scer\GAL4^sev.EP/pan^UAS.cWa is an enhancer of eye | anterior phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Scer\GAL4^sev.EP/pan^UAS.cWa is an enhancer of interommatidial bristle phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Scer\GAL4^sev.EP/pan^UAS.cWa is an enhancer of ommatidium phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

NOT Enhancer of

Statement

Reference

pan^UAS.cWa/Scer\GAL4^30A is a non-enhancer of wing phenotype of disco-r^bae-1

(Kiger et al., 2001)

Suppressor of

Statement

Reference

pan^UAS.cWa, Scer\GAL4^Act88F.PD is a suppressor | partially of wing phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2008)

Scer\GAL4^sev.EP/pan^UAS.cWa is a suppressor of rhabdomere | conditional phenotype of Hsap\HTT^GMR.Q120

(Berger et al., 2005)

pan^UAS.cWa, Scer\GAL4^30A is a suppressor of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

NOT Suppressor of

Statement

Reference

pan^UAS.cWa/Scer\GAL4^69B is a non-suppressor of embryonic/first instar larval cuticle | dorsal phenotype of hep¹

(Kaltschmidt et al., 2002)

pan^UAS.cWa/Scer\GAL4^30A is a non-suppressor of wing phenotype of disco-r^bae-1

(Kiger et al., 2001)

Scer\GAL4^bbg-C96/pan^UAS.cWa is a non-suppressor of wing phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Other

Statement

Reference

Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^sev.EP, pan^UAS.cWa has ommatidium phenotype

(Jackson et al., 2002)

Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^sev.EP, pan^UAS.cWa has eye | anterior phenotype

(Jackson et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of pan^Scer\UAS.cWa partially suppresses the gnarled wing phenotype caused by expression of E2f^Scer\UAS.cNa under the control of Scer\GAL4^Act88F.PD.

(Morris et al., 2008)

Expression of both SoxN^Scer\UAS.cOa and pan^Scer\UAS.cWa under the control of Scer\GAL4^arm.PS results in 87.6% embryonic lethality. Expression under the control of Scer\GAL4^e22c results in approximately 99.6% embryonic lethality, while expression of both SoxN^Scer\UAS.cOa and pan^Scer\UAS.cWa under the control of Scer\GAL4^dpp.blk1 results in approximately 100% pupal lethality.

Overexpression of pan^Scer\UAS.cWa enhances the repressive capacity of overexpressed SoxN^Scer\UAS.cOa. This is particularly apparent when both transgenes are expressed under the control of Scer\GAL4^arm.PS. Under these conditions, the cuticle pattern is most disrupted, both ventrally and dorsally. Ectopic denticles appear with greater frequency and broader distribution across the ventral naked cuticle domain.

(Chao et al., 2007)

pan^Scer\UAS.cWa with Scer\GAL4^69B fails to significantly suppress the dorsal hole phenotype of hep¹ embryo cuticles.

(Kaltschmidt et al., 2002)

Co-expression of arm^Scer\UAS.cSa partially suppresses the wing defects caused by expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 : the reduction in wing size, ectopic wing bristle phenotype and wing venation patterns are partially rescued, but the nicking of the wing margin is not suppressed.

Co-expression of arm^ΔC.Scer\UAS and pan^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 results in a small wing with a highly disrupted venation pattern and many ectopic bristles.

Co-expression of gro^{Nterm.Scer\UAS} in animals expressing pan^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 results in recovery of wing margin close to the hinge, but the reduced size of the wing and ectopic bristle phenotypes are not rescued.

(Lawrence et al., 2000)

pan^Scer\UAS.cWa, Scer\GAL4^C96 flies show minor wing nicking. Does not suppress wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies.

(Helms et al., 1999)

Xenogenetic Interactions

Statement

Reference

Expression of pan^Scer\UAS.cWa under the control of Scer\GAL4^sev.EP partially suppresses the loss of rhabdomeres seen in Hsap\HTT^GMR.Q120 flies.

Co-expression of pan^Scer\UAS.cWa decreases the fraction of flies with rough eyes in animals expressing Zzzz\Poly-Ala^{A37.Scer\UAS.T:Avic\GFP-EGFP,T:SV40\nls2} under the control of Scer\GAL4^ey.PB.

(Berger et al., 2005)

Hsap\MAPT^GMR.Ex.PJ and pan^Scer\UAS.cWa (driven by Scer\GAL4^hs.2sev) enhance each other leading to more roughened anterior part of the eye, with fusion, pitting, and collapse of ommatidia along with irregularity of bristles.

(Jackson et al., 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

4838

y¹ w¹¹¹⁸; P{UAS-pan.dTCF}4

4837

y¹ w¹¹¹⁸; P{UAS-pan.dTCF}24/CyO