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General Information
Symbol
Dmel\panΔN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0059983
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-TCFDN, UAS-dTCFDN, UAS-dTCFΔN, UAS-TCFΔN, UAS-dTCFΔN, UAS-DN-TCF/pan, UAS-TCF-DN, TCFDN, UAS-dnTCF, UAS-panDN
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Deletion of the N-terminal 31 amino acids.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expressing panΔN.UAS under the control of either Scer\GAL4twi.PG or Scer\GAL4Mef2.PR results in reduced numbers of cardioblasts, disrupted cardiac morphogenesis and loss of ostia. When expressed under the control of Scer\GAL4HCH.Hand, embryos have cardioblast alignment defects and lack ostia.
The adulthood-only expression of panΔN.UAS under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the almost complete absence of intestinal stem cells and of mitotic figures in the adult midgut epithelium, as compared to controls.
Expression of panΔN.Scer\UAS under the Scer\GAL4svp.PT driver results in a significant change in ostia cell shape (cells are rounder than in controls).
Expression of panΔN.UAS under the control of Scer\GAL4NP0001, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, results in an increase in cell division (PH3 staining) in the midgut and reduces lifespan, compared to controls.
Embryos expressing panΔN.Scer\UAS under the control of Scer\GAL4btl.PS show partial loss of tracheal fusion cells in the dorsal trunk, and have dorsal trunk fusion defects.
Scer\GAL4vg.PU-driven expression of panΔN.Scer\UAS leads to wing notches.
Expression of panΔN.Scer\UAS in the heart under the control of Scer\GAL4tin.CΔ4 results in flies with markedly smaller hearts and significantly reduced end diastolic dimension (EDD). Heart wall thickness is similar to controls. No accumulation of material is seen within the lumen of the heart that occupies a large portion of the cross-sectional area of the vessel chamber.
Expression of panΔN.Scer\UAS under the control of Scer\GAL41151 disrupts development of the longitudinal muscles and dorso-ventral muscles in the adult thorax.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4ebd1.PB results in a severe reduction in the size of the indirect flight muscles and a loss of flight ability.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4wg-IS650 and Scer\GAL80ts.αTub84B in the pupal wing margin results in collapse of the wing margin.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4ebd1.PB results in reduction in flight muscle volume.
Expression of panΔN.Scer\UAS under the control of a Scer\GAL4GMR.PU driver results in aberrant cone cell number and morphology 42 hours after pupal formation. The eyes of adult flies expressing panΔN.Scer\UAS under the control of Scer\GAL4GMR.PU appear glossy and have poorly defined lens facets. Expression of panΔN.Scer\UAS with the Scer\GAL4sv.PJ driver results in aberrant cone cell number and morphology 42 hours after pupal formation. Third instar larval eye discs expressing panΔN.Scer\UAS under the control of a Scer\GAL4GMR.PU driver exhibit aberrant cone cell specification. No detectable defects in photoreceptor specification are seen in the third instar eye discs of panΔN.Scer\UAS under the control of Scer\GAL4GMR.PU.
Overexpression of panΔN.Scer\UAS driven by Scer\GAL4e22c in the embryonic epidermis decreases the area of naked cuticle.
Expression of knScer\UAS.cVa under the control of Scer\GAL4kn-col85-GAL4 during lymph gland development results in the loss of posterior signaling center cells.
Developing indirect flight muscles degenerate in animals expressing panΔN.Scer\UAS under the control of Scer\GAL41151.
Intestinal stem cell clones expressing panΔN.Scer\UAS under the control of Scer\GAL4tub in the adult midgut are reduced in size compared to controls 5 days after induction.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4Act88F.PD results in a gnarled wing phenotype.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4btl.PS blocks the extension of tracheal dorsal branches, but does not disrupt epithelial integrity.
Overexpression of dominant-negative panΔN.Scer\UAS driven by Scer\GAL4byn-Gal4 in combination with Scer\GAL80ts.αTub84B results in a strong reduction of the hindgut proliferation zone in late third instar larvae. There is a decrease in proliferation and an increase in apoptosis in the hindgut proliferation zone in panΔN.Scer\UAS-overexpressing larvae compared to wild-type. If the panΔN.Scer\UAS-overexpressing animals undergo metamorphosis and eclose, the hindgut is missing entirely.
Expression of panΔN.Scer\UAS under the control of either Scer\GAL4arm.PS or Scer\GAL4e22c results in 100% embryonic lethality, while expression under the control of Scer\GAL4dpp.blk1 results in 100% pupal lethality. Expression of panΔN.Scer\UAS under the control of Scer\GAL4e22c produces a 'lawn of denticles' phenotype and severely reduces the size of the embryo.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4fkh.PZ results in salivary gland guidance defects; a large portion of the salivary gland curves ventrally towards the central nervous system, instead of lying parallel to the midline as in wild-type embryos.
Severe examples of panΔN.Scer\UAS driven by Scer\GAL4ap-md544 result in deletion of the dorsal hinge in the wing disc.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4prd.RG1 in the ectoderm of stage 11 embryos results in a reduction in the number of founder cells in prd-expressing segments. At later stages, these segments show muscle loss and morphology defects.
Ectopic expression of panΔN.Scer\UAS, under the control of Scer\GAL4vg.PM abolishes wing margin bristles.
Expression of panDeltaN.Scer\UAS in the retina behind the furrow using Scer\GAL4GMR.PF does not really affect R axon projection, with only a few mild misroutings found.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4ey.PH does not affect eye size.
When panΔN.Scer\UAS is driven by Scer\GAL4twi.PB no cluster II slou expressing muscle founder cells are seen.
Expression of panΔN.Scer\UAS, under the control of Scer\GAL4pros.PMG, does not disrupt slou founder cell clusters I and II.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4ems.HRE impairs filzkorper formation but does not affect stigmatophore formation. However, expression of this transgene under the control of Scer\GAL4salm-459.2 has the reciprocal effect; stigmatophore formation is impaired while filzkorper formation is unaffected.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4Bx-MS1096 leads to very small wings that lack a margin. Expression of panΔN.Scer\UAS under the control of Scer\GAL4Bx-MS1096 is male-specific lethal.
About 30% of panΔN.Scer\UAS; Scer\GAL4vg.PM flies have reduced wings with serrated margins.
When panΔN.Scer\UAS is driven by Scer\GAL4C96, cells in the posterior and anterior central region of the zone of nonproliferating cells (ZNC) that normally arrest in the third instar, fail to do so.
Embryos expressing panΔN.Scer\UAS under the control of Scer\GAL4da.G32 have a "lawn of denticles" cuticle phenotype.
When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is increased, lamellocyte numbers are increased and crystal cells are unaffected. Melanotic masses form. Similar, though milder, effects are seen when expression is driven by Scer\GAL4Hml.PG. When expression is driven by Scer\GAL4Sgs4.PH, there is no effect on hemocytes.
When panΔN.Scer\UAS is driven by Scer\GAL4twi.PGa misrouting of the visceral branch (VB) is seen towards the posterior region of the visceral mesoderm (VM) of each parasegment. Unlike in wild-type, no initial VB/VM contact is seen at the vicinity of the putative posterior end of the VM parasegment anterior region.
Clones in the wing disc expressing panΔN.Scer\UAS under the control of Scer\GAL4Act5C.PP grow slowly, particularly in the wing pouch during the second and third larval instar. Fragments of DNA, indicative of cell death, are repeatedly seen in cells in the clone.
When panΔN.Scer\UAS is driven by Scer\GAL4Dll-md23 the overall size of leg discs is reduced.
When panΔN.Scer\UAS is driven by Scer\GAL4C96 surviving adults have notched wings.
The dorsal rim area (DRA) of the eye remains unaffected in flies expressing panΔN.Scer\UAS under the control of Scer\GAL4GMR.PF.
panΔN.Scer\UAS expressed in clones from Scer\GAL4ptc-559.1 does not disrupt apical cell movement in the developing ovary.
Expression of panΔN.Scer\UAS in clones under the control of Scer\GAL4Act5C.PI does not suppress eye fate.
When panΔN.Scer\UAS is driven by Scer\GAL430A (and grown at 25oC) most flies die as abnormally melanized pharate adults. At 21oC many flies are able to eclose but are weak and become caught in the medium before opening their wings, Others have strongly blistered wings or have open wings that are flaccid. Some have melanotic tumours in the head or wings. Many flies have blood oozing from body joints or dried melanized blood around body joints. At 18oC many flies eclose with expanded wings and dorsal ventral surfaces that cannot bind together. These flies also have disordered and fewer hemocytes.
The indirect flight muscles of panΔN.Scer\UAS; Scer\GAL41151 flies are much reduced in size.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4da.G32 results in a dorsal cuticle that is completely devoid of 4o denticles. In the majority of embryos the dorsal cuticle is completely devoid of any denticles.
Tracheal dorsal trunk formation is inhibited in embryos expressing panΔN.Scer\UAS under the control of Scer\GAL4btl.PS.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4ptc-559.1 in embryos results in a loss of the 4o dorsal cuticle fate (fine hairs). The anterior en-expressing cells in the parasegment adopt an ectopic 1o cell row fate (dorsal denticles) and these cells are flanked anteriorly by smooth cuticle. The parasegment is narrower than normal. Occasionally, the domain of 3o cell fates (thick hairs) is extended; 5-6 rows are formed instead of the wild-type 2-3 rows.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4btl.PS results in an absence or reduction of the dorsal trunk and complete lack of branch fusion in the tracheal system. Ganglionic branches (GBs) are also misrouted; GBs associate normally with the intersegmental nerve as they grow towards the ventral nerve cord (VNC) but they often fail to switch and associate with the exit glia and the segmental nerve before entering it. These GBs remain floating in the body cavity or inside the VNC without properly associating with the segmental nerve or the longitudinal glia. Expression of panΔN.Scer\UAS under the control of Scer\GAL4salm-LP39 results in the failure of the dorsal trunk to form properly in embryos. The rest of the tracheal branches do not show major defects and migrate and fuse normally. Expression of panΔN.Scer\UAS under the control of Scer\GAL4p127 prevents many branch fusions from occurring in the tracheal system.
When expression is driven by Scer\GAL4arm.T:Hsim\VP16 the mutant embryos show dorsal closure defects.
When expression is driven by Scer\GAL4ptc-559.1, smooth cell fates in the embryonic epidermis are lost. The pattern within the denticle belt is quite normal. In place of the smooth cell fates a mirror image denticle belt forms.
When expression is driven by Scer\GAL4T155, the entire sternite and most of the tergite are transformed to pleura. A small patch of lateral tergite retains tergal identity, while more medial regions are completely transformed.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4wg.PM prevents the formation of naked cuticle in the Scer\GAL4wg.PM expression domain.
panΔN.Scer\UAS driven ubiquitously by Scer\GAL4e22c produces ectopic denticles.
When expression is driven by Scer\GAL4C96 in the ZNC of the developing wing G1 and G2 arrest are both abolished.
Embryos expressing panΔN.Scer\UAS under the control of Scer\GAL4how-24B and Scer\GAL4twi.PG show a drastic reduction or complete absence of heart progenitors, without affecting visceral mesoderm development.
Scer\GAL4e22c- or Scer\GAL469B-mediated expression causes a segment polarity phenotype.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
NOT Enhancer of
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
panΔN.UAS/Scer\GAL4ey.PH is a suppressor | partially of eye | ventral phenotype of L2
Other
Additional Comments
Genetic Interactions
Statement
Reference
The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing RetScer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is suppressed by co-expression of panΔN.Scer\UAS.
The adulthood-only co-expression of panΔN.UAS and UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the same absence of intestinal stem cells and of mitotic figures in the adult midgut epithelium as upon the expression of panΔN.UAS alone, rather than the converse increases induced by the expression of UvragHMS01357 alone.
Expression of panΔN.Scer\UAS suppresses the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.
Expression of panΔN.Scer\UAS in type II neuroblasts or ase- immature intermediate neural progenitors (INPs) suppresses the supernumerary neuroblast phenotype seen in bratDG19310/brat11 larval brains, whereas expression in ase+ immature INPs does not (different insertions of Scer\GAL4GMR9D11 are used to drive expression in each case).
AxnS044230 enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones. Apoptosis is seen both anterior and posterior to the morphogenetic furrow in the double mutants. Inhibiting wg signalling through expression of panΔN.Scer\UAS under the control of a GAL4 driver suppresses this enhancement.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4btl.PS suppresses the formation of supernumerary tracheal fusion cells which is seen in aopO199 homozygotes, with the double mutant embryos showing a loss of fusion cells similar to that seen in single mutant panΔN.Scer\UAS; Scer\GAL4btl.PS embryos.
Expression of panΔN.Scer\UAS fully suppresses the cell invasion phenotype seen in wing discs when Sin3AKK100700 is expressed under the control of Scer\GAL4ptc-559.1.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4Act.PU suppresses the increased size of Apc2g10 ApcQ8 mutant intestinal stem cell clones 7 days after clone induction, suppressing the development to hyperplasia. Multilayered clones are rarely observed, even on day 21. Ras85De1B suppresses the increased size of Apc2g10 ApcQ8 mutant intestinal stem cell clones 7 and 14 days after clone induction (ACI). The multilayering phenotype often seen at 21 days ACI is also suppressed. This reduction in cell number is due to reduced proliferation, rather than increased cell death: TUNEL expression is unchanged and expression of the apoptotic protein BacA\p35 has no effect on the phenotype. The remaining increase in clone size seen in the Ras85De1B, Apc2g10, ApcQ8 triple mutant flies is fully suppressed upon expression of panΔN.Scer\UAS under the control of Scer\GAL4Act.PU, and no increase in apoptosis is seen in these flies.
The wing phenotype - severely underdeveloped, small and deformed wings with stout bristles - characteristic for flies expressing brkScer\UAS.cJa under the control of Scer\GAL4vg.PU is partially improved by co-expression of panΔN.Scer\UAS (which on its own causes wing notches).
Co-expression of armS10.Scer\UAS.T:Hsap\MYC suppresses the loss of indirect flight muscles and loss of flight ability which is caused by expression of panΔN.Scer\UAS under the control of Scer\GAL4ebd1.PB.
The apical constriction phenotype seen posterior to the morphogenetic furrow in aopunspecified clones in the eye disc is suppressed if the clones express panΔN.Scer\UAS under the control of Scer\GAL4unspecified. The adult eye defects caused by homozygous aopunspecified clones are also suppressed if the clones express panΔN.Scer\UAS under the control of Scer\GAL4unspecified.
One copy of ewg2 enhances the indirect flight muscle defects seen when panΔN.Scer\UAS is expressed under the control of Scer\GAL4ebd1.PB.
The elimination of naked cuticle observed in Scer\GAL4e22c > panΔN.Scer\UAS embryos is unchanged in either a pavB200/+ or a tumAR2/+ mutant background.
The co-expression of panΔN.Scer\UAS suppresses the enlarged eye in adults and the increased proliferation and enlarged morphogenetic furrow in third instar larva eye discs expressing NICN.Scer\UAS driven by Scer\GAL4ey.PS.
The hyperplasia seen in AxnS044230 intestinal stem cell clones in the adult midgut is completely suppressed if they are also expressing panΔN.Scer\UAS under the control of Scer\GAL4tub. The hyperplasia seen in Apc233 ApcQ8 double mutant intestinal stem cell clones in the adult midgut is completely suppressed if they are also expressing panΔN.Scer\UAS under the control of Scer\GAL4tub. The hyperplasia seen in Apc2g10 ApcQ8 double mutant intestinal stem cell clones in the adult midgut is partially suppressed if they are also expressing panΔN.Scer\UAS under the control of Scer\GAL4tub.
Expression of panΔN.Scer\UAS enhances the tracheal phenotype seen when Src42ACA.Scer\UAS is expressed under the control of Scer\GAL4btl.PS. Tracheal cells lose polarity and dissociate from the epithelium.
The naked cuticle phenotype of nkd3 embryos is partially rescued by expression of panΔN.Scer\UAS under the control of either Scer\GAL4ptc-559.1 or Scer\GAL4en-e16E, with more complete rescue being seen when Scer\GAL4ptc-559.1 is used as the driver.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4e22c in a heterozygous SoxNNC14 results in a milder 'lawn of denticles' phenotype and also substantially rescues head cuticle defects and increases the overall size of the body compared to panΔN.Scer\UAS x Scer\GAL4e22c embryos.
The absence of wing margin bristles in mutants expressing panΔN.Scer\UAS, under the control of Scer\GAL4vg.PM is partially suppressed in ft8 heterozygotes.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4ey.PH significantly rescues the ventral eye loss of L2/+ flies in 23% of cases. Expression of either panΔN.Scer\UAS, under the control of Scer\GAL4ey.PH, in Lrev6-3 clones suppresses the loss of ventral cells in the eye disc.
The penetrance and severity of wing phenotypes in panΔN.Scer\UAS; Scer\GAL4vg.PM flies is enhanced by twsj11C8/+: 100 % of flies have severely serrated wing margins and show some degree of wing to notum transformation.
The size of clones in the wing disc expressing panΔN.Scer\UAS under the control of Scer\GAL4Act5C.PP is increased if they are coexpressing BacA\p35Scer\UAS.cHa.
Co-expression of panΔN.Scer\UAS partially suppresses the small eye and lack of interommatidial bristle phenotype caused by expression of wgwg.3'UTR.Scer\UAS under the control of Scer\GAL4GMR.PF. Co-expression of panΔN.Scer\UAS significantly rescues morphogenetic furrow progression in flies expressing wgwg.3'UTR.Scer\UAS under the control of Scer\GAL4dpp.blk1.
Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI does not suppress eye development. Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4bi-omb-Gal4 does not result in suppression of eye fate either in the dorsal or ventral margins of the eye. Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4ey.PH does not suppress eye development.
Homozygous AxnE77 clones that are also expressing panΔN.Scer\UAS under the control of Scer\GAL4αTub84B.PL differentiate normally.
The 4o dorsal cuticle fate is restored in embryos expressing panΔN.Scer\UAS under the control of Scer\GAL4ptc-559.1 if they are also coexpressing linScer\UAS.cHa; 4o type cells differentiate in place of the 3o cells, in cells flanking the en expression domain. The ectopic 1o cell row is not rescued. Anterior to this ectopic row, smooth cuticle is replaced with the 4o cell fate.
When co-expression of EgfrDN.Scer\UAS and panΔN.Scer\UAS is driven by Scer\GAL4T155 the entire tergite is transformed to pleura.
Addition of ovosvb-2 in embryos expressing panΔN.Scer\UAS driven ubiquitously by Scer\GAL4e22c suppresses both normal and panΔN.Scer\UAS induced denticles, producing mostly naked cuticle. Denticle formation induced by panΔN.Scer\UAS can be suppressed by ovoD1.Scer\UAS when driven by Scer\GAL4unspecified.
When expression is driven in the ovaries, the number of ectopic male-specific pigment cells induced by Wnt2Scer\UAS.cKa is reduced.
eyg1/Df(3L)iro-2 hemizygous eye discs completely lack photoreceptors. Photoreceptor development in these discs is rescued by panΔN.Scer\UAS expressed under the control of Scer\GAL4dpp.PH.
Xenogenetic Interactions
Statement
Reference
Co-expression of Hsap\JRKScer\UAS.cBa suppresses the loss of indirect flight muscle and loss of flight ability which is caused by expression of panΔN.Scer\UAS under the control of Scer\GAL4ebd1.PB.
The wing phenotype of panΔN.Scer\UAS/Scer\GAL4Bx-MS1096 is suppressed in a dosage-dependent fashion through expression of Hsap\MADH4100T.Scer\UAS/Scer\GAL4Bx-MS1096. The male-specific lethality observed in panΔN.Scer\UAS/Scer\GAL4Bx-MS1096 mutants is suppressed by co-expression with Hsap\MADH4100T.Scer\UAS.
BrdU incorporation in the posterior compartment of the third instar wing disc is reduced in panΔN.Scer\UAS; BacA\p35Scer\UAS.cHa; Scer\GAL4en-e16E animals. Increased BrdU incorporation in the posterior compartment of WScer\UAS.cZa; BacA\p35Scer\UAS.cHa; Scer\GAL4en-e16E is largely suppressed by panΔN.Scer\UAS.
Complementation and Rescue Data
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    References (120)