The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing RetScer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is suppressed by co-expression of panΔN.Scer\UAS.
The adulthood-only co-expression of panΔN.UAS and UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the same absence of intestinal stem cells and of mitotic figures in the adult midgut epithelium as upon the expression of panΔN.UAS alone, rather than the converse increases induced by the expression of UvragHMS01357 alone.
Expression of panΔN.Scer\UAS suppresses the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.
Expression of panΔN.Scer\UAS in type II neuroblasts or ase- immature intermediate neural progenitors (INPs) suppresses the supernumerary neuroblast phenotype seen in bratDG19310/brat11 larval brains, whereas expression in ase+ immature INPs does not (different insertions of Scer\GAL4GMR9D11 are used to drive expression in each case).
AxnS044230 enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones. Apoptosis is seen both anterior and posterior to the morphogenetic furrow in the double mutants. Inhibiting wg signalling through expression of panΔN.Scer\UAS under the control of a GAL4 driver suppresses this enhancement.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4btl.PS suppresses the formation of supernumerary tracheal fusion cells which is seen in aopO199 homozygotes, with the double mutant embryos showing a loss of fusion cells similar to that seen in single mutant panΔN.Scer\UAS; Scer\GAL4btl.PS embryos.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4Act.PU suppresses the increased size of Apc2g10 ApcQ8 mutant intestinal stem cell clones 7 days after clone induction, suppressing the development to hyperplasia. Multilayered clones are rarely observed, even on day 21.
Ras85De1B suppresses the increased size of Apc2g10 ApcQ8 mutant intestinal stem cell clones 7 and 14 days after clone induction (ACI). The multilayering phenotype often seen at 21 days ACI is also suppressed. This reduction in cell number is due to reduced proliferation, rather than increased cell death: TUNEL expression is unchanged and expression of the apoptotic protein BacA\p35 has no effect on the phenotype. The remaining increase in clone size seen in the Ras85De1B, Apc2g10, ApcQ8 triple mutant flies is fully suppressed upon expression of panΔN.Scer\UAS under the control of Scer\GAL4Act.PU, and no increase in apoptosis is seen in these flies.
The wing phenotype - severely underdeveloped, small and deformed wings with stout bristles - characteristic for flies expressing brkScer\UAS.cJa under the control of Scer\GAL4vg.PU is partially improved by co-expression of panΔN.Scer\UAS (which on its own causes wing notches).
Co-expression of armS10.Scer\UAS.T:Hsap\MYC suppresses the loss of indirect flight muscles and loss of flight ability which is caused by expression of panΔN.Scer\UAS under the control of Scer\GAL4ebd1.PB.
The apical constriction phenotype seen posterior to the morphogenetic furrow in aopunspecified clones in the eye disc is suppressed if the clones express panΔN.Scer\UAS under the control of Scer\GAL4unspecified. The adult eye defects caused by homozygous aopunspecified clones are also suppressed if the clones express panΔN.Scer\UAS under the control of Scer\GAL4unspecified.
The co-expression of panΔN.Scer\UAS suppresses the enlarged eye in adults and the increased proliferation and enlarged morphogenetic furrow in third instar larva eye discs expressing NICN.Scer\UAS driven by Scer\GAL4ey.PS.
The hyperplasia seen in Apc233 ApcQ8 double mutant intestinal stem cell clones in the adult midgut is completely suppressed if they are also expressing panΔN.Scer\UAS under the control of Scer\GAL4tub.
The hyperplasia seen in Apc2g10 ApcQ8 double mutant intestinal stem cell clones in the adult midgut is partially suppressed if they are also expressing panΔN.Scer\UAS under the control of Scer\GAL4tub.
Expression of panΔN.Scer\UAS enhances the tracheal phenotype seen when Src42ACA.Scer\UAS is expressed under the control of Scer\GAL4btl.PS. Tracheal cells lose polarity and dissociate from the epithelium.
The naked cuticle phenotype of nkd3 embryos is partially rescued by expression of panΔN.Scer\UAS under the control of either Scer\GAL4ptc-559.1 or Scer\GAL4en-e16E, with more complete rescue being seen when Scer\GAL4ptc-559.1 is used as the driver.
Expression of panΔN.Scer\UAS under the control of Scer\GAL4e22c in a heterozygous SoxNNC14 results in a milder 'lawn of denticles' phenotype and also substantially rescues head cuticle defects and increases the overall size of the body compared to panΔN.Scer\UAS x Scer\GAL4e22c embryos.
The penetrance and severity of wing phenotypes in panΔN.Scer\UAS; Scer\GAL4vg.PM flies is enhanced by twsj11C8/+: 100 % of flies have severely serrated wing margins and show some degree of wing to notum transformation.
Co-expression of panΔN.Scer\UAS partially suppresses the small eye and lack of interommatidial bristle phenotype caused by expression of wgwg.3'UTR.Scer\UAS under the control of Scer\GAL4GMR.PF. Co-expression of panΔN.Scer\UAS significantly rescues morphogenetic furrow progression in flies expressing wgwg.3'UTR.Scer\UAS under the control of Scer\GAL4dpp.blk1.
Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI does not suppress eye development. Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4bi-omb-Gal4 does not result in suppression of eye fate either in the dorsal or ventral margins of the eye. Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4ey.PH does not suppress eye development.
The 4o dorsal cuticle fate is restored in embryos expressing panΔN.Scer\UAS under the control of Scer\GAL4ptc-559.1 if they are also coexpressing linScer\UAS.cHa; 4o type cells differentiate in place of the 3o cells, in cells flanking the en expression domain. The ectopic 1o cell row is not rescued. Anterior to this ectopic row, smooth cuticle is replaced with the 4o cell fate.
Addition of ovosvb-2 in embryos expressing panΔN.Scer\UAS driven ubiquitously by Scer\GAL4e22c suppresses both normal and panΔN.Scer\UAS induced denticles, producing mostly naked cuticle. Denticle formation induced by panΔN.Scer\UAS can be suppressed by ovoD1.Scer\UAS when driven by Scer\GAL4unspecified.
When expression is driven in the ovaries, the number of ectopic male-specific pigment cells induced by Wnt2Scer\UAS.cKa is reduced.