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General Information
Symbol
Dmel\nej3
Species
D. melanogaster
Name
FlyBase ID
FBal0059993
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dCBP3
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

2-3kb deletion near the 5' end of the nej gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous nej3 significantly blocks or reduces tolerance to both benzyl alcohol and ethanol.

nej3 does not show any dominant suppressor (Su(var)) or enhancer (E(var)) of variegation activity. Any second site Su(var) mutations on the original chromosome can be separated from nej3 by recombination. Additional copies of nej+mSa (or nejT:SV5\V5) fail to rescue the Su(var) phenotype of the original chromosome carrying nej3.

Small homozygous clones can be recovered at a low frequency in the wing disc if they are induced in a Minute/+ background.

Mutants show developmental arrest soon after the prepupal-pupal transition, with persistent larval salivary glands.

The severe rough eye phenotype caused by expression of nejScer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF is suppressed if the flies also carry nej3/+. The very mild rough eye phenotype caused by expression of nejKIX.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed if the flies also carry nej3/+. The moderate rough eye phenotype caused by expression of nejΔNZK.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced if the flies also carry nej3/+. The severe rough eye phenotype caused by expression of nejΔBHQ.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced if the flies also carry nej3/+. The mild rough eye phenotype caused by expression of nejΔHQ.Scer\UAS under the control of Scer\GAL4GMR.PF is not affected if the flies also carry nej3/+. The severe rough eye phenotype caused by expression of nejΔQ.Scer\UAS under the control of Scer\GAL4hs.2sev is not affected if the flies also carry nej3/+.

Homozygous embryos that die at stage 9 often have a characteristic twisted phenotype.

Mutant stage 17 embryos do not show significant defects in the location or formation of the tracheal dorsal trunk branch, dorsal branch 10, spiracular branch 10 or the posterior spiracle.

Gastric caecae frequently fail to elongate. Formation of the first midgut constriction is often impeded. Dorsal trunk and visceral branches of the developing trachea are essentially normal. Branching defects occur, typically one of two dorsal branches fail to form and ganglionic branches fail to fuse.

Only 15% of nej3 hemizygotes die as embryos.

Mutant embryos look comparatively normal though demonstrate midgut abnormalities reminiscent of sgg mutants. The midgut constriction is present. Epidermis of embryos shows frequent loss of anterior denticles of the denticle belt, similar to the phenotype seen for mild over-activation of wg.

Embryos appear twisted at germ band elongation, clockwise or anticlockwise just behind the cephalic furrow, often with the posterior side down. The ventral and cephalic furrows appear normal, but the mesodermally derived internal tissues and the block of ectodermal cells are often missing. Embryos die at stage 9 or 10 during embryogenesis, some embryos survive to hatch. Cuticles of late-lethal embryos exhibit defects. No eggs are produced from germ line clones.

Hemizygous embryos generally die at stage 9 or 10, although some survive to hatching. Embryos are twisted from 0-70% egg length at germband elongation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

nej[+]/nej3 is an enhancer of visible | dominant phenotype of soD

nej3 is an enhancer of increased cell size | pupal stage phenotype of Myb1

nej3 is an enhancer of cell shape defective | pupal stage phenotype of Myb1

nej3 is an enhancer of lethal phenotype of Myb1/Myb2

nej3 is an enhancer of lethal phenotype of Myb2

nej3 is an enhancer of lethal phenotype of Myb1

NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

nej[+]/nej3 is a suppressor of visible | dominant phenotype of gcmPyx

nej[+]/nej3 is a suppressor | partially of visible | dominant phenotype of ciD

Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

nej3 has denticle belt | anterior phenotype, suppressible by arm2

Enhancer of
Statement
Reference

nej[+]/nej3 is an enhancer of eye disc phenotype of soD

nej[+]/nej3 is an enhancer of eye phenotype of soD

nej3 is an enhancer of adult cuticle phenotype of Myb1

nej3 is an enhancer of adult abdominal segment phenotype of Myb1

nej3 is an enhancer of nuclear chromosome phenotype of Myb1

nej3 is an enhancer of wing & cell phenotype of Myb1

nej3 is an enhancer of wing & nucleus phenotype of Myb1

nej3 is an enhancer of wing hair | precursor | supernumerary phenotype of Myb1

nej3 is an enhancer of wing hair | precursor phenotype of Myb1

nej3 is an enhancer of abdomen phenotype of Myb1

nej3 is an enhancer of wing vein L4 phenotype of dpps1

nej3 is an enhancer of wing vein L2 phenotype of dpps1

nej[+]/nej3 is an enhancer of haltere & macrochaeta | ectopic phenotype of UbxM1

NOT Enhancer of
Statement
Reference

nej[+]/nej3 is a non-enhancer of adult thorax | pharate adult stage phenotype of chm14

nej3 is a non-enhancer of centrosome phenotype of Myb1

nej3, Scer\GAL430A is a non-enhancer of wing phenotype of Pka-C1UAS.cKa, Scer\GAL430A

Suppressor of
Statement
Reference

nej[+]/nej3 is a suppressor of chaeta | supernumerary phenotype of gcmPyx

nej3 is a suppressor of wing margin phenotype of ciD

nej[+]/nej3 is a suppressor of wing phenotype of Scer\GAL4en-e16E, shgUAS.cSa

nej[+]/nej3 is a suppressor of wing phenotype of ciD

nej[+]/nej3 is a suppressor of trichome of the posterior wing margin phenotype of ciD

NOT Suppressor of
Statement
Reference

nej[+]/nej3 is a non-suppressor of adult thorax | pharate adult stage phenotype of chm14

nej3, Scer\GAL430A is a non-suppressor of wing phenotype of Pka-C1UAS.cKa, Scer\GAL430A

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The number of mitotic cells is slightly but significantly higher in hydroxyurea-treated discs from mei-41RT1/nej3 transheterozygotes compared with heterozygous mei-41RT1 controls.

Expression of nej+t16 suppresses the mitosis phenotypes in response to hydroxyurea seen in mei-41RT1/nej3 transheterozygotes.

nej3/+ ; soD/+ adults lack eyes and the nondifferentiated field of tissue that is seen in place of the eye in soD/+ single mutants is replaced by head tissue in the double mutant adults. nej3/+ ; soD/+ eye imaginal discs are small and undergo increased levels of cell death.

When nej3 is added to Myb1 animals at 18oC a dramatic reduction in viability is seen as well as a significantly delayed emergence of surviving animals. When nej3 is added to Myb1/Myb2 animals an even more marked effect is seen on viability and emergence. When nej3 is added to Myb1 animals at 18oC no effect is seen on viability or any delay in development seen. When nej3/+ is added to Myb1 animals at 18oC an enhancement of the multiple wing hair phenotype is seen. The abdomen defects are also enhanced. White differentiated cuticle is present between most segments and along the dorsal midline, indicating fusion defects. Defects in wing cell and nucleus size are also enhanced, nuclei and cells are larger and more variable in size. The mitotic index phenotype is also enhanced in these mutants, though no increase in the rate of centrosomal and chromosomal defects is seen.

Double mutants for sggM11 and nej3 show a worse gut phenotype than do nej3 single mutants. The embryonic epidmeris phenotype is suppressed in double mutants with arm2. Dominantly suppresses the wing margin phenotype of shgScer\UAS.cSa, Scer\GAL4en-e16E.

Suppresses a subset of the ciD wing defects (loss of posterior row wing hairs, abnormal wing shape) in ciD/+ nej3/+ transheterozygotes.

Xenogenetic Interactions
Statement
Reference

One copy of nej3 enhances the reduction in eclosion rate seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155. These flies have a relative eclosion rate of 67.3%.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (31)