Allele Dmel\tup^Scer\UAS.cTa

General Information
Symbol	Dmel\tup^Scer\UAS.cTa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Thor	FlyBase ID	FBal0060384
Feature type	allele	Associated gene	Dmel\tup

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Thor and Thomas, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A truncated tup cDNA (nucleotides 425 to 2982) is expressed under the control of Scer\UAS regulatory sequences. (Thor and Thomas, 1997)
Carried in construct	P{UAS-isl.T} (de Navascués and Modolell, 2007, Broihier and Skeath, 2002, Pueyo and Couso, 2004, Thor and Thomas, 1997, Biryukova and Heitzler, 2005, Hari et al., 2008, Boukhatmi et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| embryonic stage, with Scer\GAL4^sca-537.4 (Thor and Thomas, 1997) visible, with Scer\GAL4^C-765 (de Navascués and Modolell, 2007)
Phenotype Manifest In
	A1-7 dorsal acute muscle 2 \| ectopic, with Scer\GAL4^twi.PB (Boukhatmi et al., 2012) A1-7 dorsal acute muscle 3, with Scer\GAL4^twi.PB (Boukhatmi et al., 2012) dorsocentral bristle, with Scer\GAL4^pnr-MD237 (Biryukova and Heitzler, 2005) femur, with Scer\GAL4^dpp.blk1 (Pueyo and Couso, 2004) interneuron \| embryonic stage, with Scer\GAL4^sca-537.4 (Thor and Thomas, 1997) mesothoracic pleurum, with Scer\GAL4^C-765 (de Navascués and Modolell, 2007) metatarsus, with Scer\GAL4^dpp.blk1 (Pueyo and Couso, 2004) motor neuron \| embryonic stage, with Scer\GAL4^sca-537.4 (Thor and Thomas, 1997) scutellar bristle, with Scer\GAL4^pnr-MD237 (Biryukova and Heitzler, 2005) tarsal segment 4, with Scer\GAL4^ap-md544 (Pueyo and Couso, 2004) tegula, with Scer\GAL4^C-765 (de Navascués and Modolell, 2007) thorax, with Scer\GAL4^pnr-MD237 (Biryukova and Heitzler, 2005) tibia, with Scer\GAL4^dpp.blk1 (Pueyo and Couso, 2004) unguis, with Scer\GAL4^dpp.blk1 (Pueyo and Couso, 2004) wing, with Scer\GAL4^ap-md544 (Biryukova and Heitzler, 2005)
Detailed Description
	Statement Reference Embryos expressing tup[Scer\UAS.cTa] under the control of Scer\GAL4[twi.PB] show a transformation of muscle DA3 to DA2 in 88% of segments. (Boukhatmi et al., 2012) Expression of tup^Scer\UAS.cTa under the control of Scer\GAL4^C-765 results in the formation of notum-like tissue in the mesothoracic pleura and extra notum-like bristles on the tegula. (de Navascués and Modolell, 2007) Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^pnr-MD237, affects the development of the dorsocentral and scutellar bristles and induces a thoracic cleft. Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^ap-md544, results in the formation of rudimentary wings. (Biryukova and Heitzler, 2005) The legs of tup^Scer\UAS.cTa; Scer\GAL4^ap-md544 flies lack a fourth tarsal segment. tup^Scer\UAS.cTa; Scer\GAL4^dpp.blk1 flies the femur, tibia and first tarsal segment are fused. In the pretarsus only one claw develops. (Pueyo and Couso, 2004) tup^isl-1 mutants expressing tup^Scer\UAS.cTa using Scer\GAL4^elav.PLu do not hatch. Produces changes in axonal projections of interneurons and motor neurons, when expressed using Scer\GAL4^sca-537.4. Motor neurons that normally innervate dorsal muscles often remain within the ventral muscle region. Both ISN and SNa motor axons often fail to reach their normal target areas and show abnormal arborisations over the ventral muscle region. (Thor and Thomas, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference tup^Scer\UAS.cTa, Scer\GAL4^Ccap.PP is a suppressor of eclosion defective phenotype of Bx^EP1306, Scer\GAL4^Ccap.PP (Hari et al., 2008)
Other
Statement Reference Scer\GAL4^C-765, ara^Scer\UAS.cGa, tup^Scer\UAS.cTa has visible phenotype (de Navascués and Modolell, 2007) Scer\GAL4^dpp.blk1, ara^Scer\UAS.cGa, tup^Scer\UAS.cTa has visible phenotype (de Navascués and Modolell, 2007)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^ap-md544, tup^Scer\UAS.cTa has tarsal segment 4 phenotype, suppressible \| partially by B^Scer\UAS.cKa, Scer\GAL4^ap-md544 (Pueyo and Couso, 2004) Scer\GAL4^ap-md544, tup^Scer\UAS.cTa has wing phenotype, suppressible by ap^Scer\UAS.cOa/Scer\GAL4^ap-md544 (Biryukova and Heitzler, 2005)
NOT suppressed by
Statement Reference Scer\GAL4^ap-md544, tup^Scer\UAS.cTa has tarsal segment 4 phenotype, non-suppressible by ap^Scer\UAS.cOa/Scer\GAL4^ap-md544 (Pueyo and Couso, 2004) Scer\GAL4^ap-md544, tup^Scer\UAS.cTa has tarsal segment 4 phenotype, non-suppressible by Chi^{FL.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^ap-md544 (Pueyo and Couso, 2004)
Suppressor of
Statement Reference tup^Scer\UAS.cTa, Scer\GAL4^Ccap.PP is a suppressor of wing phenotype of Bx^EP1306, Scer\GAL4^Ccap.PP (Hari et al., 2008) tup^Scer\UAS.cTa/Scer\GAL4^pnr-MD237 is a suppressor \| partially of scutellar bristle \| supernumerary phenotype of Chi^E (Biryukova and Heitzler, 2005)
Other
Statement Reference Scer\GAL4^C-765, ara^Scer\UAS.cGa, tup^Scer\UAS.cTa has wing hinge phenotype (de Navascués and Modolell, 2007) Scer\GAL4^C-765, ara^Scer\UAS.cGa, tup^Scer\UAS.cTa has wing phenotype (de Navascués and Modolell, 2007) Scer\GAL4^dpp.blk1, ara^Scer\UAS.cGa, tup^Scer\UAS.cTa has wing phenotype (de Navascués and Modolell, 2007)
Additional Comments
Genetic Interactions
Statement Reference Expression of tup[Scer\UAS.cTa] with Bx[EP1306], both under the control of Scer\GAL4[Ccap.PP], suppresses the wing expansion defect found upon expression of Bx[EP1306] alone. (Hari et al., 2008) Co-expression of both tup^Scer\UAS.cTa and ara^Scer\UAS.cGa under the control of Scer\GAL4^C-765 results in the wing and wing hinge being replaced by a large structure of notum-like tissue. Co-expression of both tup^Scer\UAS.cTa and ara^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 transforms the central part of the wing to notum-like tissue, whereas the anterior and posterior parts develop as wing tissue. The animals also show notum-like structures near the coxa, indicating transformation of the ventral region of the body wall towards notum. (de Navascués and Modolell, 2007) Coexpression of the ap^Scer\UAS.cOa transgene rescues the rudimentary wing phenotype of Scer\GAL4^ap-md544>tup^Scer\UAS.cTa flies. Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^ap-md544, in a Chi^E background causes no wing defects. Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^pnr-MD237, partially suppresses the excess scutellar bristle phenotype of Chi^E mutants. (Biryukova and Heitzler, 2005) The loss of tarsal segment 4 in the legs of tup^Scer\UAS.cTa; Scer\GAL4^ap-md544 flies is not suppressed by ap^Scer\UAS.cOa or Chi^{FL.Scer\UAS.T:Hsap\MYC}, but is partially suppressed by B^Scer\UAS.cKa. (Pueyo and Couso, 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
Bloomington	39662 w^; P{UAS-isl.T}10 39660 P{UAS-isl.T}16F, w^ 39661 w^*; P{UAS-isl.T}4M/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	isl^UAS.cTa tup^Scer\UAS.cTa tup^UAS.cTa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Thor (Thor and Thomas, 1997)
Secondary FlyBase IDs

References ( 7 )
Research paper	Boukhatmi et al., 2012, Development 139(19): 3572--3582 Tup/Islet1 integrates time and position to specify muscle identity in Drosophila. [FBrf0219376] Hari et al., 2008, J. Neurosci. 28(37): 9145--9150 Chip is required for posteclosion behavior in Drosophila. [FBrf0206079] de Navascués and Modolell, 2007, Development 134(9): 1779--1788 tailup, a LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification. [FBrf0201638] Biryukova and Heitzler, 2005, Dev. Biol. 288(2): 559--570 The Drosophila LIM-homeodomain protein Islet antagonizes proneural cell specification in the peripheral nervous system. [FBrf0190092] Pueyo and Couso, 2004, Development 131(13): 3107--3120 Chip-mediated partnerships of the homeodomain proteins Bar and Aristaless with the LIM-HOM proteins Apterous and Lim1 regulate distal leg development. [FBrf0179395] Broihier and Skeath, 2002, Neuron 35(1): 39--50 Drosophila homeodomain protein dHb9 directs neuronal fate via crossrepressive and cell-nonautonomous mechanisms. [FBrf0151450] Thor and Thomas, 1997, Neuron 18(3): 397--409 The Drosophila islet gene governs axon pathfinding and neurotransmitter identity. [FBrf0093227]

Allele Dmel\tupScer\UAS.cTa

Allele Dmel\tup^Scer\UAS.cTa