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General Information
Symbol
Dmel\fzUAS.cAa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Adler
FlyBase ID
FBal0060399
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Fz, UASFz
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of fz.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

sensory mother cell & spindle, with Scer\GAL4ap-md52

wing & microchaeta, with Scer\GAL4ptc-559.1

wing & microchaeta | distal, with Scer\GAL4Dll-md23

wing hair & 1st posterior cell, with Scer\GAL4ptc-559.1

Detailed Description
Statement
Reference

Expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 in the wing results in non-autonomous hair reorientation, with hairs outside the Scer\GAL4dpp.blk1 expression domain orienting away from it.

Expression of fzScer\UAS.cAa using Scer\GAL4hs.2sev results in ommatidial orientation defects in the eye.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.PS results in planar polarity defects in the wing.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4en-e16E results in a very mild multiple wing hair phenotype.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4en-e16E results in wing hairs pointing from the posterior to the anterior compartment in the wing.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev results in planar cell polarity defects in the eye, including a high number of symmetrical ommatidia containing two R3 cells.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4ptc-559.1 results in wing hairs pointing away from the midline within the expression domain. Outside of the expression domain wing hair polarity is normal.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev results in planar cell polarity defects in the eye.

Expression of fzScer\UAS.cAa in the border cells under the control of Scer\GAL4slbo.2.6 delays border cell migration. A reduction in the number of large actin-protrusions from the border cell cluster compared to wild-type clusters is observed.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev results in planar polarity defects in the eye; misrotated ommatidia and symmetrical nonchiral ommatidia are seen (only 44.9% of ommatidia are correctly rotated).

The planar polarity defects seen in the eyes of animals expressing fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev are almost completely suppressed by fz30 (76.0% of ommatidia are correctly rotated).

Only 30.3% of ommatidia are correctly rotated in flies expressing fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev and also co-expressing fzarm.T:Avic\GFP-EGFP.

The ommatidia of fzScer\UAS.cAa; Scer\GAL4sev.PM181 adult eyes have random chirality and some are symmetrical, with two R3 photoreceptors in place of an R3/R4 pair.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 in a stripe along the proximo-distal axis of the wing results in a reorientation of wing hairs, which now point laterally away from the expression domain.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 results in reorientation of wing hairs away from the domain of fz expression.

fzScer\UAS.cAa; Scer\GAL4dpp.blk1 flies have a zone extending anterior to the dpp expression domain in which wing hairs point away from the fz overexpressing cells.

When expression is driven by Scer\GAL4Dll-md23, such that fz expression decreases away from the distal part of the wing, the wing hairs point proximally, instead of distally.

When fzScer\UAS.cAa is driven by Scer\GAL4ptc-559.1 in a stripe along the proximal distal axis of the wing, a reorganized polarity results in those cells, that is perpendicular to the normal axis. The wing hairs in the region between wing vein L3 and L4, distal to the anterior crossvein point away from the midline of the wing, unlike wild-type wings, where the wing hairs point distally.

Expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 in the wing results in reorientation of the hairs from high to low levels of fz expression.

When fzScer\UAS.cAa is driven by Scer\GAL4hs.2sev, planar polarity defects are seen in the ommatidia.

Expression of fzScer\UAS.cAa driven by Scer\GAL4109(2)80 does not give rise to any detectable dendritic phenotypes.

When expression is driven by Scer\GAL4hs.2sev, ommatidia are often mis-rotated and show chirality defects. Most of the symmetrical clusters are R3/R3 symmetrical.

fzScer\UAS.cAa driven by Scer\GAL4ap-md52 disrupts the planar polarity of bristles in the notum, primarily affecting microchaetae. Induction of ectopic scutellar bristles are also seen about 80% of the time. In the pupal notum of flies expressing fzScer\UAS.cAa driven by Scer\GAL4ap-md52, the spindle orientations of the Sensory organ precursors (SOP) become randomized.

When expressed in the wing under the control of Scer\GAL4ptc-559.1, wing hairs are orientated away from the highest point of the fz gradient.

Embryos expressing fzScer\UAS.cAa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 have a lawn of denticles phenotype.

Scer\GAL4Dll-md23-mediated expression causes a region of reversed polarity near the distal end of the wing. Scer\GAL4ptc-559.1-mediated expression causes a region of reverse polarity in anterior compartment cells along the anterior-posterior compartment border.

Causes a tissue polarity defect when expressed in a subset of photoreceptors in the developing eye using Scer\GAL4hs.2sev; ommatidia frequently show abnormal rotation and chirality.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4hs.2sev, fzUAS.cAa has ommatidium phenotype, suppressible | partially by tow[+]/tow754

Scer\GAL4hs.2sev, fzUAS.cAa has ommatidium phenotype, suppressible | partially by JraIA109/Jra[+]

Scer\GAL4hs.2sev, fzUAS.cAa has ommatidium phenotype, suppressible | partially by Jra76-19/Jra[+]

NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference

fzUAS.cAa/Scer\GAL4Dll-md23 is a suppressor of wing hair & 1st posterior cell phenotype of in1

Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of fwGD3327 suppresses the non-autonomous hair reorientation caused by expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.blk1 in the wing.

Co-expression of gishGD10588 with fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev enhances the ommatidial rotation defects induced by Scer\GAL4hs.2sev>fzScer\UAS.cAa.

The planar polarity defects in the wing caused by expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.PS are partially suppressed by VhaM8.9EY03616 or VhaM8.95-HA-1890, but are not suppressed by VhaM8.9EY03616-excΔ.

Co-expression of fzScer\UAS.cAa enhances the penetrance of the multiple wing hair phenotype caused by expression of zipRod.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4en-e16E. In addition, the double mutant wings also show polarity defects.

The planar polarity defects in the eye which are caused by expression of fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev are partially suppressed by VhaM8.9EY03616/+ or by co-expression of VhaM8.9KK107676, but are not suppressed by VhaM8.9EY03616-ex.

Co-expression of VhaM8.9Scer\UAS.T:Disc\RFP-mCherry enhances the planar polarity defects in the eye which are caused by expression of fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev.

The planar cell polarity defects in the eye that are caused by expression of fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev are partially suppressed if the flies also simultaneously co-express Nhe2dsRNA.Scer\UAS.WIZ and carry Nhe21/+.

The planar polarity defects seen in the eyes of animals expressing fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev are almost completely suppressed by tow754/+ (79.7% of ommatidia are correctly rotated) or In(3L)towfz/+ (79.0% of ommatidia are correctly rotated).

The planar polarity defects seen in the eyes of animals expressing fzScer\UAS.cAa under the control of Scer\GAL4hs.2sev are enhanced by co-expression of towScer\UAS.cCa (only 26.4% of ommatidia are correctly rotated).

Co-expression of Wnt4Scer\UAS.cGa suppresses the change in wing hair polarity caused by expression of fzScer\UAS.cAa under the control of Scer\GAL4dpp.blk1.

fzScer\UAS.cAa; Scer\GAL4dpp.blk1 flies have a zone extending anterior to the dpp expression domain in which wing hairs point away from the fz overexpressing cells. When such wings have somatic clones of ftG-rv in the anterior of the wing, this zone enlarges to fill the clone.

The planar polarity defect in dsh1 wings that are expressing fzScer\UAS.cAa under the control of Scer\GAL471B is indistinguishable from the dsh1 single mutant phenotype.

The tissue polarity phenotype caused by expression of fzScer\UAS.cAa using Scer\GAL4hs.2sev is dominantly suppressed by fz1, dsh3, Rho1k07236, Rho1k07903, bsk1 and bsk2, but is not affected by rl10, pkpk-sple-13, nmoE33 or wgl-17.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Rescues the embryonic cuticle phenotype of double mutants of fz21 or fz23 with Df(3L)fz2.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
fzScer\UAS.cAa
fzUAS.cAa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Adler
Secondary FlyBase IDs
    References (34)