Open Close
General Information
Symbol
Dmel\JraUAS.cEa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Eresh
FlyBase ID
FBal0060634
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-jun, UAS-djun
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of full-length Jra.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of JraScer\UAS.cEa under the control of Scer\GAL4Hand.ΔVM.Switch (initiated by feeding flies RU486 during adulthood) results in cardiac function defects. Heart period (mean time between successive end-diastolic positions) is increased in 3 day and 10 day old flies compared to age-matched controls. No difference is seen in older flies.

Expression of JraScer\UAS.cEa in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) has no significant effect on RP2 dendrite volume.

Expression of JraScer\UAS.cEa under the control of either Scer\GAL4pnr-MD237, Scer\GAL4ey.PH or Scer\GAL4how-24B does not cause a phenotype.

Expression of JraScer\UAS.cEa under the control of Scer\GAL4elav-C155 does not affect EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction. mEJC amplitude is not significantly altered.

Flies expressing JraScer\UAS.cEa under the control of Scer\GAL4tim.Scer\UAS have wild-type locomotor activity rhythms.

Causes no effect on copper cell development, when expression is driven by Scer\GAL448Y.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4D42 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) results in a significant increase in total dendritic length and in the number of dendritic branches in MN5 motorneurons. The length and radii of individual dendrite segments and dendritic territory borders are unaffected.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4103.3 results in a significant increase in total dendritic length and in the number of dendritic branches in MN5 motorneurons. The length and radii of individual dendrite segments and dendritic territory borders are unaffected.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4futsch-C380 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) results in a significant decrease in total dendritic length and in the number of dendritic branches in MN5 motorneurons. The length and radii of individual dendrite segments are unaffected. The dendrites do not reach the normal neuropil boundaries and appear less ramified with several parts missing. The most severe defects are in the proximal-posterior region.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4gfA-3295 results in a significant reduction in the number of adult MN5 dendrites.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4futsch-C380 results in significantly fewer dendritic branches in the MN5 motorneurons. If expression is delayed past pupal stage P6 by using the temperature-sensitive Scer\GAL80ts.αTub84B allele, then no obvious dendritic defects are seen.

The mutant eye phenotype caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is exacerbated by co-expression of JraScer\UAS.cEa.

Co-expression of JraScer\UAS.cEa reduces tumor and metastasis incidence in the 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa pan-neurally under the control of Scer\GAL4elav-C155 positively regulates synaptic strength.

Co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 increases synaptic size (measured as the number of synaptic boutons) compared to wild-type controls.

Co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa rescues the small eye phenotype seen upon expression of kayFbz.Scer\UAS in the eye disc using Scer\GAL4ey.3.5.Exel.

Co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 increases synaptic size (measured as the number of synaptic boutons) compared to wild-type controls.

Expression of sggEY02862 suppresses the reduced EJC amplitude found upon expression of kayScer\UAS.cEa and JraScer\UAS.cEa pan-neurally under the control of Scer\GAL4elav-C155.

Co-expression of cnkEY06675 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of Fkbp13EY02405 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of Pde8EY10143 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of sggEY02862 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of SdcEY04602 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of Sema-2aEY08184 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, does not affect synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of both kayScer\UAS.cEa and JraScer\UAS.cEa in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) results in a significant increase in RP2 dendrite volume compared to controls.

Cultured motor neurons co-expressing both kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4futsch-C380 (in the presence of Scer\GAL80Cha.PK to suppress expression in cholinergic neurons) show a significant increase in both neurite length and branch number compared to controls.

Simultaneous co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa under the control of Scer\GAL4elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

Co-expression of JraScer\UAS.cEa partially suppresses the symmetrical ommatidia phenotype caused by expression of pntP2.Scer\UAS under the control of Scer\GAL4hs.2sev.

Coexpression of Mnn1Scer\UAS.cCa and JraScer\UAS.cEa under the control of either Scer\GAL4pnr-MD237, Scer\GAL4ey.PH or Scer\GAL4how-24B results in the same phenotypes as expression of Mnn1Scer\UAS.cCa alone.

Expression of both kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4elav-C155 results in a 30% increase in EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction. mEJC amplitude is not significantly altered. Expression of CrebB-17Ab.hs does not change the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on bouton number, but significantly inhibits the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on EJC amplitude (synaptic strength); the triple mutant flies have an EJC amplitude 108% that of control flies. Expression of dncScer\UAS.cCa under the control of Scer\GAL4elav-C155 does not change the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on bouton number, but significantly inhibits the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on EJC amplitude (synaptic strength); the triple mutant flies have an EJC amplitude 83% that of control flies. Expression of pucScer\UAS.cMa under the control of Scer\GAL4elav-C155 suppresses the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on bouton number and EJC amplitude (synaptic strength) at the larval neuromuscular junction.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
JraScer\UAS.cEa
JraUAS.cEa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Eresh
Secondary FlyBase IDs
    References (18)