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General Information
Symbol
Dmel\Hsp83P582
Species
D. melanogaster
Name
FlyBase ID
FBal0060640
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P582
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
P-element insertion within the 5' untranslated region, 29bp downstream of the transcription start site.
Insertion components
P{}Hsp83P582
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Hsp8313F3/Hsp83P582 transheterozygous neuroblasts exhibit loss of apical cortical polarity at prophase.
A Hsp83P582 background has a highly significant and reproducible effect on total phenotypic variation of scutellar and thoracic bristles. Twice as many Hsp83P582 mutant flies exhibit abnormal thoracic bristle numbers, with the severity of the thoracic bristle number defects increasing from deviations of at most two thoracic bristles to as many as four. The number of flies with aberrant scutellar bristle numbers increases 4 to 5-fold in Hsp83P582 mutants.
Hsp83P582/Hsp839J1 mutants develop until larval or early pupae. Larval brains of these mutants exhibit mitotic defects. Polyploid and other aneuploid cells make up about 30% of the mitotic figures seen. About 20% of anaphases are disorganised. The centrosome cycle is also found to be impaired in these cells. There are two classes of defect. Mitotic cells are seen containing a single microtubule organising centre (MTOC) made up of one or two unsegregated centrosomes, leading to the organisation of monopolar mitotic figures. Cells are also seen in which the centrosomes appear to fail in their assembly.
Hsp8308445/Hsp83P582 males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.
Lethality occurs at embryonic or early larval stages. Mutation interacts genetically with the sev mutations; 'sev315' and sevS11.T:Hsap\MYC.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference
Hsp83P582/Hsp83[+] is a suppressor of arista | supernumerary phenotype of obk1
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially complements
Comments
Hsp83P582 complements Hsp8308445 for viability and female fertility but not for male fertility.
Lethality can be rescued by Hsp83+t7.5. Hsp83+t7.5 also rescues the dominant suppression phenotype.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Excision of the P-element reverts the lethal phenotype.
Precise excision of the P-element demonstrates the insertion is responsible for the lethal phenotype.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)