The mechanical stress sensitivity of Tpisgk-1 animals is significantly suppressed if they are also expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR (in the presence of Mifepristone).
Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF in a auxI670K/+ background generates a severe rough eye phenotype, more severe than expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF in a wild-type background.
Co-expression of Hsc70-4K71S.Scer\UAS and Rme-8fl.Scer\UAS.T:Disc\RFP-mRFP, under the regulation of Scer\GAL4GMR.PF, during eye development enhances the ommatidial disorganisation observed in Hsc70-4K71S.Scer\UAS (regulated by Scer\GAL4GMR.PF). In addition, a decrease in eye size is also observed, suggesting that the rough eye phenotype of Hsc70-4K71S.Scer\UAS (regulated by Scer\GAL4GMR.PF) is enhanced by Rme-8fl.Scer\UAS.T:Disc\RFP-mRFP expression. Expression of Rme-8ΔJ.Scer\UAS.T:Disc\RFP-mRFP, under the regulation of Scer\GAL4GMR.PF has little effect on the Hsc70-4K71S.Scer\UAS (under the control of Scer\GAL4GMR.PF) phenotype. Expression of auxillinfl.Scer\UAS, under the regulation of Scer\GAL4GMR.PF, has little or no effect on the rough eye phenotype of Hsc70-4K71S.Scer\UAS (expressed under the control of Scer\GAL4GMR.PF).
Co-expression of Hsc70-4K71S.Scer\UAS with Scer\GAL4GMR.PF>Hsap\GJB3F137L.Scer\UAS.T:Hsap\MYC leads to further deleterious eye phenotypes manifesting in complete loss of pigmentation and increased black degeneration lesions.
The progressive degeneration and pigmentation loss eye phenotype characteristic for flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is enhanced by co-expression of Hsc70-4K71S.Scer\UAS.
Expression of Hsc70-4K71S.Scer\UAS in flies expressing Hsap\HDQ108.ex1p.Scer\UAS under the control of Scer\GAL4elav-C155 in a CrebB-17A+tIa transgene background increases the survival rate from approximately 10% in Hsap\HDQ108.ex1p.Scer\UAS (Scer\GAL4elav-C155) CrebB-17A+tIa mutants, to over 100%, indicating a synergistic effect between Hsc70-4K71S.Scer\UAS and CrebB-17A+tIa.
Co-expression of Hsc70-4K71S.Scer\UAS accelerates the loss of dopaminergic neurons caused by expression of Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL; neuronal loss is apparent in 1 day old flies expressing both Hsc70-4K71S.Scer\UAS and Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL whereas neuronal loss in flies overexpressing only Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL occurs by 10 to 20 days.
Co-expression of Hsc70-4K71S.Scer\UAS with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF enhances the eye phenotype, such that the flies show a severely degenerated eye, whereas expression of Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 alone mildly affects the eye.
Co-expression of Pros261.Scer\UAS and Hsc70-4K71S.Scer\UAS, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) enhances the eye phenotype.
Expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG cannot suppress the severe eye degeneration seen in flies co-expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 and Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PU.