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General Information
Symbol
Dmel\Hsc70-4K71S.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0060645
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Hsc4.K71S, UAS-Hsc70-4K71S, UAS-HSC70-4.K71S
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: K71S.
Encodes a Hsc70-4 protein with a single point mutation in the ATP binding site.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4btl.PS results in disorganisation of the chitin extracellular matrix in the embryonic tracheal lumens. Lumen clearance is defective in these embryos, resulting in a failure of gas filling.
Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF results in a rough eye phenotype.
Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C results in increased cell size in imaginal discs.
Expression of Hsc70-4K71S.Scer\UAS, under the regulation of Scer\GAL4GMR.PF, during eye development results in disruption of the regular arrays of ommatidia and a roughening of the eye.
A modest effect on the eye is seen when Hsc70-4K71S.Scer\UAS is driven by Scer\GAL4GMR.PF.
Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.
20 day old (but not 1 day old) flies expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4Ddc.PL show loss of dopaminergic neurons.
Animals expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4337Y die as first or second instar larvae. Animals expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4how-24B generally die as pupae or larvae (depending on the P{UAS-Hsc70-4.K71S} line used).
Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF has no effect on the eye.
Dominant lethal allele.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
NOT Enhanced by
NOT suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
The mechanical stress sensitivity of Tpisgk-1 animals is significantly suppressed if they are also expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR (in the presence of Mifepristone).
Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF in a auxI670K/+ background generates a severe rough eye phenotype, more severe than expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF in a wild-type background.
Co-expression of Hsc70-4K71S.Scer\UAS and Rme-8fl.Scer\UAS.T:Disc\RFP-mRFP, under the regulation of Scer\GAL4GMR.PF, during eye development enhances the ommatidial disorganisation observed in Hsc70-4K71S.Scer\UAS (regulated by Scer\GAL4GMR.PF). In addition, a decrease in eye size is also observed, suggesting that the rough eye phenotype of Hsc70-4K71S.Scer\UAS (regulated by Scer\GAL4GMR.PF) is enhanced by Rme-8fl.Scer\UAS.T:Disc\RFP-mRFP expression. Expression of Rme-8ΔJ.Scer\UAS.T:Disc\RFP-mRFP, under the regulation of Scer\GAL4GMR.PF has little effect on the Hsc70-4K71S.Scer\UAS (under the control of Scer\GAL4GMR.PF) phenotype. Expression of auxillinfl.Scer\UAS, under the regulation of Scer\GAL4GMR.PF, has little or no effect on the rough eye phenotype of Hsc70-4K71S.Scer\UAS (expressed under the control of Scer\GAL4GMR.PF).
Xenogenetic Interactions
Statement
Reference
Co-expression of Hsc70-4K71S.Scer\UAS with Scer\GAL4GMR.PF>Hsap\GJB3F137L.Scer\UAS.T:Hsap\MYC leads to further deleterious eye phenotypes manifesting in complete loss of pigmentation and increased black degeneration lesions.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 induced loss of rhabdomeres and significantly reduces the percentage of cells with aggregates.
The progressive degeneration and pigmentation loss eye phenotype characteristic for flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is enhanced by co-expression of Hsc70-4K71S.Scer\UAS.
Expression of Hsc70-4K71S.Scer\UAS in flies expressing Hsap\HDQ108.ex1p.Scer\UAS, both under the control of Scer\GAL4elav-C155, increases the survival rate from almost 0% to over 80%. The presence of the Hsc70-4K71S.Scer\UAS transgene does partially suppress Hsap\HDQ108.ex1p.Scer\UAS-mediated locomotor dysfunction (when both are expressed under the control of Scer\GAL4elav-C155). Expression of Hsc70-4K71S.Scer\UAS in flies expressing Hsap\HDQ108.ex1p.Scer\UAS under the control of Scer\GAL4elav-C155 in a CrebB-17A+tIa transgene background increases the survival rate from approximately 10% in Hsap\HDQ108.ex1p.Scer\UAS (Scer\GAL4elav-C155) CrebB-17A+tIa mutants, to over 100%, indicating a synergistic effect between Hsc70-4K71S.Scer\UAS and CrebB-17A+tIa.
The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying Hsc70-4K71S.Scer\UAS.
Co-expression of Hsc70-4K71S.Scer\UAS accelerates the loss of dopaminergic neurons caused by expression of Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL; neuronal loss is apparent in 1 day old flies expressing both Hsc70-4K71S.Scer\UAS and Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL whereas neuronal loss in flies overexpressing only Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL occurs by 10 to 20 days.
Co-expression of Hsc70-4K71S.Scer\UAS with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF enhances the eye phenotype, such that the flies show a severely degenerated eye, whereas expression of Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 alone mildly affects the eye. Co-expression of Pros261.Scer\UAS and Hsc70-4K71S.Scer\UAS, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) enhances the eye phenotype.
Expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG cannot suppress the severe eye degeneration seen in flies co-expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 and Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PU.
Enhances the disruption of the eye caused by expression of Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Hsc70-4K71S.Scer\UAS
Hsc70-4K71S.UAS
Hsc70-4K71S
Name Synonyms
Secondary FlyBase IDs
    References (22)