Allele Dmel\Hsc70-3^{K97S.Scer\UAS}

General Information
Symbol	Dmel\Hsc70-3^{K97S.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0060647
Feature type	allele	Associated gene	Dmel\Hsc70-3

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Elefant and Palter, 1997, Elefant and Palter, 1999) in vitro construct - regulatory fusion (Elefant and Palter, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Hsc70-3^Scer\UAS.cEa (Elefant and Palter, 1999)
Nature of the lesion	Statement Reference Amino acid replacement: K97S. (Elefant and Palter, 1999) Construct: Encodes a Hsc70-3 protein with a single point mutation in the ATP binding site. (Elefant and Palter, 1997)
Carried in construct	P{UAS-Hsc70-3.K97S} (Chang et al., 2004, Elefant and Palter, 1999, Elefant and Palter, 1995, Yamakawa et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^how-24B (Elefant and Palter, 1999) lethal \| dominant, with Scer\GAL4^unspecified (Elefant and Palter, 1997) lethal \| larval stage, with Scer\GAL4^337Y (Elefant and Palter, 1999)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Chang et al., 2004) ommatidium, with Scer\GAL4^GMR.PF (Chang et al., 2004)
Detailed Description
	Statement Reference Expression of Hsc70-3^{K97S.Scer\UAS} in the eye discs under the regulation of Scer\GAL4^GMR.PF causes a slight deformation and roughening of the eye. (Chang et al., 2004) Animals expressing Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^337Y die as first or second instar larvae. Animals expressing Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^how-24B generally die as pupae or larvae (depending on the P{UAS-Hsc70-3.K97S} line used). (Elefant and Palter, 1999) Dominant lethal allele. (Elefant and Palter, 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Hsc70-3^{K97S.Scer\UAS}/Scer\GAL4^arm.PS is a suppressor \| partially of neurogenic phenotype \| maternal effect \| embryonic stage phenotype of pcx³ (Yamakawa et al., 2012)
Phenotype Manifest In
Suppressor of
Statement Reference Hsc70-3^{K97S.Scer\UAS}/Scer\GAL4^arm.PS is a suppressor of endoplasmic reticulum \| maternal effect phenotype of pcx³ (Yamakawa et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Expression of Hsc70-3[K97S.Scer\UAS] under the control of Scer\GAL4[arm.PS] in pcx[3] embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 29% of embryos are fully rescued, 38% are partially rescued and 33% are not rescued. The endoplasmic reticulum defects are also rescued in 67% of cases. (Yamakawa et al., 2012) Coexpression of Hsc70-3^{K97S.Scer\UAS} and Rme-8^{fl.Scer\UAS.T:Disc\RFP-mRFP}, both under the regulation of Scer\GAL4^GMR.PF has little or no effect on the Hsc70-3^{K97S.Scer\UAS} phenotype. (Chang et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	5842 w¹²⁶; P{UAS-Hsc70-3.K97S}D
Kyoto	108460 w¹²⁶; P{UAS-Hsc70-3.K97S}D
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	HSC3^K97S (Elefant and Palter, 1997) Hsc70-3^{K97S.Scer\UAS} Hsc70-3^K97S.UAS K97S (Elefant and Palter, 1995)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Yamakawa et al., 2012, Development 139(3): 558--567 Deficient Notch signaling associated with neurogenic pecanex is compensated for by the unfolded protein response in Drosophila. [FBrf0217160] Chang et al., 2004, J. Cell Biol. 164(7): 1055--1064 The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in Drosophila. [FBrf0174799] Elefant and Palter, 1999, Mol. Biol. Cell 10(7): 2101--2117 Tissue-specific expression of dominant negative mutant Drosophila HSC70 causes developmental defects and lethality. [FBrf0109657]
Abstract	Elefant and Palter, 1997, A. Dros. Res. Conf. 38: 86C Targeted gene expression of dominant mutant HSC3 and HSC4 genes in Drosophila. [FBrf0091838] Elefant and Palter, 1995, Mol. Biol. Cell 6(Suppl.): 65a Targeted gene expression of mutant HSC3 and HSC4 genes in Drosophila. [FBrf0084997]

Allele Dmel\Hsc70-3K97S.Scer\UAS

Allele Dmel\Hsc70-3^{K97S.Scer\UAS}