FlyBase Allele Report: Scer\GAL4[sli.PS]

General Information

Symbol

Scer\GAL4^sli.PS

Species

S. cerevisiae

Name

slit promoter construct of Scholz

FlyBase ID

FBal0060665

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-sli.S}

Also Known As

Slit-GAL4, sli-GAL4, slitGal4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Scholz et al., 1997, Scholz et al., 1997, Albagli et al., 1996)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GAL4-sli.S}

Cytology

Nature of the lesion

Statement

Reference

1kb EcoRI fragment of sli promoter sequences regulate expression of a GAL4 driver.

(Scholz et al., 1997)

Allele components

Product class / Tool use(s)

Regulatory region(s)

sli

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 17

midline glial cell

(Scholz et al., 1997)

embryonic stage 13 -- 17

MP1 neuron

(Scholz et al., 1997)

embryonic stage

midline glial cell

(Albagli et al., 1996)

Additional Information

Statement

Reference

Drives expression in embryonic midline glial cells.

(FlyBase Curators, 2017)

In embryos, conspicuous expression in midline glial cells, initially observed in presumptive midline glial cells visible during germ band retraction. From stage 13/14, expression also observed in MP1 neurons. In late stage 16, expression also observed in unidentified ventrally-located cells.

(Scholz et al., 1997)

Marker for

midline glial cell

(Scholz et al., 1997)

Reflects expression of

sli

(Scholz et al., 1997, Albagli et al., 1996)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon, with sli^UAS.cBa

(Battye et al., 1999)

central nervous system, with sli^UAS.cBa

(Battye et al., 1999)

commissure, with aop^ACT.UAS

(Scholz et al., 1997, Scholz et al., 1997)

commissure, with robo2^{10xUAS.Tag:HA,Tag:SS(wg)}

(Evans et al., 2015)

commissure, with robo2^{ΔC.10xUAS.Tag:HA,Tag:SS(wg)}

(Evans et al., 2015)

commissure, with sli^UAS.cBa

(Battye et al., 1999)

dendrite, with sli^GD5822

(Brierley et al., 2009)

longitudinal connective, with robo1::robo2^{R2I1+2.10xUAS.Tag:HA,Tag:SS(wg)}

(Evans et al., 2015)

longitudinal connective, with robo1^{10xUAS.Tag:HA,Tag:SS(wg)}

(Evans et al., 2015)

longitudinal connective, with robo1^{ΔC.10xUAS.Tag:HA,Tag:SS(wg)}

(Evans et al., 2015)

longitudinal connective, with robo2^{10xUAS.Tag:HA,Tag:SS(wg)}

(Evans et al., 2015)

longitudinal connective, with robo2^{ΔC.10xUAS.Tag:HA,Tag:SS(wg)}

(Evans et al., 2015)

longitudinal connective, with sli^UAS.cBa

(Battye et al., 1999)

midline glial cell, with Egfr^DN.UAS

(Bergmann et al., 2002)

midline glial cell, with hid^Ala5.UAS

(Bergmann et al., 2002)

midline glial cell | ectopic, with Raf^UAS.F179

(Scholz et al., 1997, Scholz et al., 1997)

midline glial cell | ectopic, with Ras85D^V12.UAS

(Scholz et al., 1997, Scholz et al., 1997)

midline ventral glial cell | ectopic, with jing^UAS.cSa

(Sonnenfeld et al., 2004)

motor neuron, with sli^GD5822

(Brierley et al., 2009)

neuropil, with sli^GD5822

(Brierley et al., 2009)

ventral nerve cord, with sli^UAS.cBa

(Battye et al., 2001)

ventral nerve cord, with sli^ΔE2-E6.UAS

(Battye et al., 2001)

ventral nerve cord, with sli^ΔG-E7.UAS

(Battye et al., 2001)

ventral nerve cord commissure, with hid^Ala5.UAS

(Bergmann et al., 2002)

ventral nerve cord commissure, with jing^UAS.cSa

(Sonnenfeld et al., 2004)

ventral nervous system, with sli^UAS.cBa

(Battye et al., 1999)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^sli.PS has lethal phenotype, suppressible by spen^ΔC.UAS, Scer\GAL4^sli.PS

(Chen and Rebay, 2000)

Scer\GAL4^sli.PS, spen^ΔC.UAS has lethal phenotype, suppressible by Ras85D^V12.UAS, Scer\GAL4^sli.PS

(Chen and Rebay, 2000)

Scer\GAL4^sli.PS, spen^ΔC.UAS has lethal phenotype, suppressible by Scer\GAL4^sli.PS/spi^s.UAS

(Chen and Rebay, 2000)

Suppressor of

Statement

Reference

spen^ΔC.UAS, Scer\GAL4^sli.PS is a suppressor of lethal phenotype of Ras85D^V12.UAS, Scer\GAL4^sli.PS

(Chen and Rebay, 2000)

Ras85D^V12.UAS, Scer\GAL4^sli.PS is a suppressor of lethal phenotype of Scer\GAL4^sli.PS, spen^ΔC.UAS

(Chen and Rebay, 2000)

Scer\GAL4^sli.PS/spi^s.UAS is a suppressor of lethal phenotype of Scer\GAL4^sli.PS, spen^ΔC.UAS

(Chen and Rebay, 2000)

NOT Suppressor of

Statement

Reference

dlp^UAS.cBa/Scer\GAL4^sli.PS is a non-suppressor of abnormal neuroanatomy phenotype of Sdc⁴⁸

(Johnson et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sli.PS, jing^UAS.cSa has midline ventral glial cell | ectopic phenotype, suppressible by S^IIN/S^IIN

(Sonnenfeld et al., 2004)

Scer\GAL4^sli.PS, jing^UAS.cSa has midline ventral glial cell | ectopic phenotype, suppressible by spi¹/spi¹

(Sonnenfeld et al., 2004)

Egfr^DN.UAS, Scer\GAL4^sli.PS has midline glial cell phenotype, suppressible by hid^rvX1/hid^rvX1

(Bergmann et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^sli.PS, jing^UAS.cSa has midline ventral glial cell | ectopic phenotype, non-suppressible by S[+]/S^IIN

(Sonnenfeld et al., 2004)

Scer\GAL4^sli.PS, jing^UAS.cSa has midline ventral glial cell | ectopic phenotype, non-suppressible by spi¹/spi[+]

(Sonnenfeld et al., 2004)

Suppressor of

Statement

Reference

rho^UAS.cBa/Scer\GAL4^sli.PS is a suppressor | partially of embryonic/larval ganglionic branch phenotype of ru^unspecified

(Gallio et al., 2004)

ALV\v-ets^UAS.cAa/Scer\GAL4^sli.PS is a suppressor | partially of midline glial cell phenotype of pnt^Δ88

(Albagli et al., 1996)

Ggal\ets1^UAS.cAa/Scer\GAL4^sli.PS is a suppressor | partially of midline glial cell phenotype of pnt^Δ88

(Albagli et al., 1996)

Scer\GAL4^sli.PS/Ggal\ets2^UAS.cAa is a suppressor | partially of midline glial cell phenotype of pnt^Δ88

(Albagli et al., 1996)

NOT Suppressor of

Statement

Reference

dlp^UAS.cBa/Scer\GAL4^sli.PS is a non-suppressor of presumptive embryonic/larval central nervous system phenotype of Sdc⁴⁸

(Johnson et al., 2004)

jing^UAS.cSa/Scer\GAL4^sli.PS is a non-suppressor of midline ventral glial cell phenotype of S^IIN

(Sonnenfeld et al., 2004)

jing^UAS.cSa/Scer\GAL4^sli.PS is a non-suppressor of midline ventral glial cell phenotype of spi¹

(Sonnenfeld et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

siz^P2.UAS/Scer\GAL4^sli.PS rescues siz^U112

(Onel et al., 2004)

siz^P1.UAS/Scer\GAL4^sli.PS rescues siz^U112

(Onel et al., 2004)

Scer\GAL4^sli.PS/pnt^P1.UAS rescues pnt^Δ88

(Scholz et al., 1997)

pnt^P2.UAS/Scer\GAL4^sli.PS rescues pnt^Δ88

(Scholz et al., 1997)

Scer\GAL4^sli.PS/pnt^P1.UAS rescues pnt^Δ88

(Scholz et al., 1997)

pnt^P2.UAS/Scer\GAL4^sli.PS rescues pnt^Δ88

(Scholz et al., 1997)

Partially rescues

Scer\GAL4^sli.PS/sli^Uncleave.UAS partially rescues sli^unspecified

(Coleman et al., 2010)

sli^UAS.cUa/Scer\GAL4^sli.PS partially rescues sli^unspecified

(Coleman et al., 2010)

comm^UAS.cKa/Scer\GAL4^sli.PS partially rescues comm¹

(Georgiou and Tear, 2002)

sli^ΔG-E7.UAS/Scer\GAL4^sli.PS partially rescues sli²

(Battye et al., 2001)

sli^ΔE2-E6.UAS/Scer\GAL4^sli.PS partially rescues sli²

(Battye et al., 2001)

Scer\GAL4^sli.PS/sli^UAS.cBa partially rescues sli²

(Battye et al., 2001)

sli^UAS.cKa/Scer\GAL4^sli.PS partially rescues sli²

(Kidd et al., 1999)

ttk^p69.UAS/Scer\GAL4^sli.PS partially rescues ttk^B330

(Giesen et al., 1997)

Fails to rescue

kuz^UAS.Tag:HA/Scer\GAL4^sli.PS fails to rescue kuz^unspecified

(Coleman et al., 2010)

kuz^UAS.cCa/Scer\GAL4^sli.PS fails to rescue kuz^unspecified

(Coleman et al., 2010)

Scer\GAL4^sli.PS/kra^UAS.Tag:HA fails to rescue kra²/kra¹

(Lee et al., 2007)

Scer\GAL4^sli.PS/Sdc^UAS.cJa fails to rescue Sdc⁴⁸

(Johnson et al., 2004)

comm^UAS.cKa/Scer\GAL4^sli.PS fails to rescue comm^E39

(Georgiou and Tear, 2002)

sli^ΔL1-L4.UAS/Scer\GAL4^sli.PS fails to rescue sli²

(Battye et al., 2001)

Hem^UAS.cHa/Scer\GAL4^sli.PS fails to rescue Hem^unspecified

(Hummel et al., 2000)

pnt^T151A.UAS/Scer\GAL4^sli.PS fails to rescue pnt^Δ88

(Scholz et al., 1997)

pnt^T151A.UAS/Scer\GAL4^sli.PS fails to rescue pnt^Δ88

(Scholz et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9580

w^*; P{GAL4-sli.S}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^sli.PS

Scer\GAL4^sli.PS

Name Synonyms

slit promoter construct of Scholz

(Scholz et al., 1997, Scholz et al., 1997, Albagli et al., 1996)

Secondary FlyBase IDs

References (36)

Report Sections

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