FlyBase Allele Report: Scer\GAL4[sim.P3.7]

General Information

Symbol

Scer\GAL4^sim.P3.7

Species

S. cerevisiae

Name

FlyBase ID

FBal0060666

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-sim.3.7}

Also Known As

sim-Gal4, P[sim-Gal4], simGAL4, 3.7sim-Gal4, single-minded (sim)-Gal4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Xiao et al., 1996)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GAL4-sim.3.7}

Cytology

Nature of the lesion

Statement

Reference

A 3.7kb BamHI fragment of sim 5' flanking DNA containing the early sim promoter and 5' flanking sequences (encodes 18 amino acids of coding sequence) regulates expression of a GAL4 driver.

(Xiao et al., 1996)

Allele components

Product class / Tool use(s)

Regulatory region(s)

sim

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 17

ventral nerve cord | precursor

(Xiao et al., 1996)

ventral midline of embryo

(Xiao et al., 1996)

embryonic stage 11

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 13

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 17

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

embryonic stage

embryonic midline of ventral nerve cord

(Bloomington Drosophila Stock Center, 2017)

embryonic stage 11 -- 12

ventral midline of embryo

(Fontana and Crews, 2012)

embryonic stage 16

ventral midline of embryo

(Fontana and Crews, 2012)

Additional Information

Statement

Reference

Drives expression in cells of the ventral midline of the embryonic CNS.

(FlyBase Curators, 2017)

Midline expression is present in all midline cells from segments S1 to A8/9.

(Fontana and Crews, 2012)

Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.

(Wheeler et al., 2006)

In embryos, expression specific to CNS midline precursor cells (CMP); not expressed in ventral ectodermal cells.

(Xiao et al., 1996)

Marker for

ventral midline of embryo

(Xiao et al., 1996)

Reflects expression of

sim

(Bloomington Drosophila Stock Center, 2017, Wheeler et al., 2006, Xiao et al., 1996)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Abcd1^HMS02382

(Gordon et al., 2018)

decreased cell number | adult stage, with Elovl7^HMC03112

(Gordon et al., 2018)

neuroanatomy defective, with Abcd1^HMS02382

(Gordon et al., 2018)

neuroanatomy defective, with Elovl7^GD16713

(Gordon et al., 2018)

neuroanatomy defective, with Elovl7^HMC03112

(Gordon et al., 2018)

neuroanatomy defective, with en^UAS.cGa

(Watson et al., 2011)

neuroanatomy defective, with hh^UAS.cAa

(Watson et al., 2011)

neuroanatomy defective, with jing^UAS.cSa

(Sedaghat and Sonnenfeld, 2002)

neuroanatomy defective, with sim^UAS.cXa

(Sedaghat and Sonnenfeld, 2002)

neuroanatomy defective, with tgo^Δc.UAS

(Sonnenfeld et al., 2005)

neuroanatomy defective | embryonic stage 13, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

neuroanatomy defective | embryonic stage 13, with spi^s.UAS

(Zhang et al., 2011)

neuroanatomy defective | embryonic stage 13, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

neuroanatomy defective | embryonic stage 16, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

neuroanatomy defective | embryonic stage 16, with spi^s.UAS

(Zhang et al., 2011)

neuroanatomy defective | embryonic stage 16, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

neuroanatomy defective | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

neuroanatomy defective | embryonic stage, with D^EnR.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

neuroanatomy defective | embryonic stage, with D^UAS.WT

(Shen et al., 2013)

neuroanatomy defective | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

neuroanatomy defective | embryonic stage, with D^ΔHMG.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

neuroanatomy defective | embryonic stage, with Mmus\Sox2^{EnR.UAS.Tag:MYC}

(Shen et al., 2013)

neuroanatomy defective | embryonic stage, with Mmus\Sox2^UAS.EYFP

(Shen et al., 2013)

neuroanatomy defective | embryonic stage, with Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC}

(Shen et al., 2013)

viable, with Abcd1^HMS02382

(Gordon et al., 2018)

Phenotype Manifest In

A1-7 dorsal acute muscle 1, with sli², sli^UAS.cKa

(Kramer et al., 2001)

A1-7 dorsal acute muscle 2, with sli², sli^UAS.cKa

(Kramer et al., 2001)

A1-7 dorsal oblique muscle 1, with sli², sli^UAS.cKa

(Kramer et al., 2001)

A1-7 dorsal oblique muscle 2, with sli², sli^UAS.cKa

(Kramer et al., 2001)

A1-7 lateral oblique muscle 1, with sli², sli^UAS.cKa

(Kramer et al., 2001)

A1-7 ventral longitudinal muscle 3, with sli², sli^UAS.cKa

(Kramer et al., 2001)

A1-7 ventral longitudinal muscle 4, with sli², sli^UAS.cKa

(Kramer et al., 2001)

anterior commissure | embryonic stage, with Inx7^dsRNA.UAS

(Ostrowski et al., 2008)

axon, with sli^UAS.cBa

(Battye et al., 1999)

axon | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

axon | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

central nervous system, with sli^UAS.cBa

(Battye et al., 1999)

commissure, with jing^UAS.cSa

(Sedaghat et al., 2002)

commissure, with sli^UAS.cBa

(Battye et al., 1999)

commissure, with tgo^Δc.UAS

(Sonnenfeld et al., 2005)

commissure | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

commissure | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

embryonic/larval central nervous system, with D^EnR.UAS

(Shen et al., 2013)

embryonic/larval central nervous system, with D^EnR.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

embryonic/larval central nervous system, with D^UAS.WT

(Shen et al., 2013)

embryonic/larval central nervous system, with D^ΔHMG.UAS

(Shen et al., 2013)

embryonic/larval central nervous system, with D^ΔHMG.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

embryonic/larval central nervous system, with Mmus\Sox2^{EnR.UAS.Tag:MYC}

(Shen et al., 2013)

embryonic/larval central nervous system, with Mmus\Sox2^UAS.EYFP

(Shen et al., 2013)

embryonic/larval central nervous system, with Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC}

(Shen et al., 2013)

embryonic/larval somatic muscle | ectopic, with sli², sli^UAS.cKa

(Kramer et al., 2001)

embryonic brain, with jing^UAS.cSa

(Sedaghat and Sonnenfeld, 2002)

embryonic brain, with sim^UAS.cXa

(Sedaghat and Sonnenfeld, 2002)

longitudinal connective, with jing^UAS.cSa

(Sedaghat et al., 2002)

longitudinal connective, with sli^UAS.cBa

(Battye et al., 1999)

longitudinal connective, with tgo^Δc.UAS

(Sonnenfeld et al., 2005)

longitudinal connective | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

longitudinal connective | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

longitudinal connective | embryonic stage, with Inx7^dsRNA.UAS

(Ostrowski et al., 2008)

midline glial cell, with en^UAS.cGa

(Watson et al., 2011)

midline glial cell, with hh^UAS.cAa

(Watson et al., 2011)

midline glial cell | embryonic stage 13 | supernumerary, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

midline glial cell | embryonic stage 13 | supernumerary, with spi^s.UAS

(Zhang et al., 2011)

midline glial cell | embryonic stage 13 | supernumerary, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

midline glial cell | embryonic stage 14, with Mmmm\ras^N17.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | supernumerary, with Egfr^U.A887T.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | supernumerary, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

midline glial cell | embryonic stage 16 | supernumerary, with Rat\Neu^NT.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | supernumerary, with Rat\Neu^YB.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | supernumerary, with Rat\Neu^YC.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | supernumerary, with Rat\Neu^YD.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | supernumerary, with Rat\Neu^YE.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | supernumerary, with spi^s.UAS

(Zhang et al., 2011)

midline glial cell | embryonic stage 16 | supernumerary, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

pigment cell, with Abcd1^HMS02382

(Gordon et al., 2018)

pigment cell, with Elovl7^HMC03112

(Gordon et al., 2018)

posterior commissure | embryonic stage, with Inx7^dsRNA.UAS

(Ostrowski et al., 2008)

retina, with Abcd1^HMS02382

(Gordon et al., 2018)

retina, with Elovl7^GD16713

(Gordon et al., 2018)

retina, with Elovl7^HMC03112

(Gordon et al., 2018)

SP1 neuron, with sli^UAS.cBa

(Battye et al., 1999)

tract | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

tract | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

ventral nerve cord | embryonic stage, with Inx7^dsRNA.UAS

(Ostrowski et al., 2008)

ventral nervous system, with sli^UAS.cBa

(Battye et al., 1999)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, mam^ΔC has neuroanatomy defective | embryonic stage 16 phenotype, enhanceable by N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has neuroanatomy defective | embryonic stage 13 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has neuroanatomy defective | embryonic stage 16 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has neuroanatomy defective | embryonic stage 13 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has neuroanatomy defective | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7 has neuroanatomy defective | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^sim.P3.7/Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC} is an enhancer of neuroanatomy defective | embryonic stage phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Mmus\Sox2^{EnR.UAS.Tag:MYC}/Scer\GAL4^sim.P3.7 is an enhancer of neuroanatomy defective | embryonic stage phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7 is an enhancer of neuroanatomy defective | embryonic stage 16 phenotype of Scer\GAL4^sim.P3.7, mam^ΔC

(Zhang et al., 2011)

NOT Enhancer of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-enhancer of neuroanatomy defective | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Suppressor of

Statement

Reference

Mmus\Sox2^UAS.EYFP/Scer\GAL4^sim.P3.7 is a suppressor of neuroanatomy defective | embryonic stage phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

dlp^UAS.cBa/Scer\GAL4^sim.P3.7 is a suppressor | partially of neuroanatomy defective | embryonic stage phenotype of Sdc²³

(Chanana et al., 2009)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor | partially of neuroanatomy defective phenotype of jing³/Df(2R)ST1

(Sonnenfeld et al., 2004)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor | partially of neuroanatomy defective phenotype of jing³

(Sonnenfeld et al., 2004)

Ras85D^V12.UAS/Scer\GAL4^sim.P3.7 is a suppressor | partially of neuroanatomy defective phenotype of jing³

(Sonnenfeld et al., 2004)

NOT Suppressor of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-suppressor of neuroanatomy defective | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | supernumerary | embryonic stage 13 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | supernumerary | embryonic stage 16 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | supernumerary | embryonic stage 13 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | supernumerary | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7 has midline glial cell | supernumerary | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Egfr^U.A887T.UAS, Scer\GAL4^sim.P3.7 has midline glial cell | supernumerary | embryonic stage 16 phenotype, non-suppressible by Rat\Neu^KDYD.UAS, Scer\GAL4^sim.P3.7

(Settle et al., 2003)

Enhancer of

Statement

Reference

Scer\GAL4^sim.P3.7/Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC} is an enhancer of embryonic/larval central nervous system phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Mmus\Sox2^{EnR.UAS.Tag:MYC}/Scer\GAL4^sim.P3.7 is an enhancer of embryonic/larval central nervous system phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Scer\GAL4^sim.P3.7/N^{Intra1768.UAS.Tag:FLAG} is an enhancer of midline glial cell | supernumerary | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

NOT Enhancer of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-enhancer of midline glial cell | supernumerary | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Suppressor of

Statement

Reference

Mmus\Sox2^UAS.EYFP/Scer\GAL4^sim.P3.7 is a suppressor of embryonic/larval central nervous system phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

dlp^UAS.cBa/Scer\GAL4^sim.P3.7 is a suppressor | partially of fascicle | ectopic | embryonic stage phenotype of Sdc²³

(Chanana et al., 2009)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor | partially of midline ventral glial cell phenotype of jing³/Df(2R)ST1

(Sonnenfeld et al., 2004)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor | partially of midline ventral glial cell phenotype of jing³

(Sonnenfeld et al., 2004)

Ras85D^V12.UAS/Scer\GAL4^sim.P3.7 is a suppressor | partially of midline ventral glial cell phenotype of jing³

(Sonnenfeld et al., 2004)

NOT Suppressor of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-suppressor of midline glial cell | supernumerary | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Rat\Neu^KDYD.UAS, Scer\GAL4^sim.P3.7 is a non-suppressor of midline glial cell | supernumerary | embryonic stage 16 phenotype of Egfr^U.A887T.UAS, Scer\GAL4^sim.P3.7

(Settle et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

bgm^UAS.Tag:FLAG/Scer\GAL4^sim.P3.7 rescues bgm¹

(Gordon et al., 2018)

sli^UAS.cKa/Scer\GAL4^sim.P3.7 rescues sli²

(Orgogozo et al., 2004)

Partially rescues

NetA^UAS.cHa/Scer\GAL4^sim.P3.7 partially rescues Df(1)NetAB^Δ

(Newquist et al., 2013)

Scer\GAL4^sim.P3.7/NetB^UAS.cHa partially rescues Df(1)NetAB^Δ

(Newquist et al., 2013)

comm^UAS.cKa/Scer\GAL4^sim.P3.7 partially rescues comm¹

(Georgiou and Tear, 2002)

sli^UAS.cKa/Scer\GAL4^sim.P3.7 partially rescues sli²

(Kramer et al., 2001)

sli^UAS.cKa/Scer\GAL4^sim.P3.7 partially rescues sli²

(Kramer et al., 2001)

Scer\GAL4^sim.P3.7/sli^UAS.cBa partially rescues sli²

(Battye et al., 1999)

rho^UAS.cXa/Scer\GAL4^sim.P3.7 partially rescues rho^7M43

(Xiao et al., 1996)

Fails to rescue

NetA^UAS.cHa/Scer\GAL4^sim.P3.7 fails to rescue Df(1)NetAB^Δ

(Newquist et al., 2013)

Sdc^{PA.UAS.Tag:FLAG,GFP}/Scer\GAL4^sim.P3.7 fails to rescue Sdc²³

(Chanana et al., 2009)

β-Spec^UAS.Tag:MYC/Scer\GAL4^sim.P3.7 fails to rescue β-Spec^em6

(Garbe et al., 2007)

Dl^UAS.cDa/Scer\GAL4^sim.P3.7 fails to rescue Dl^vi1/Dl^6B

(Crowner et al., 2003)

comm^UAS.cKa/Scer\GAL4^sim.P3.7 fails to rescue comm^E39

(Georgiou and Tear, 2002)

rho^UAS.cXa/Scer\GAL4^sim.P3.7 fails to rescue rho^PΔ38

(Lanoue and Jacobs, 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

26784

w^*; P{GAL4-sim.3.7}2, P{UAS-GFP.btau}2

9150

w^*; P{GAL4-sim.3.7}2/CyO; P{GAL4-sim.3.7}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GAL4^sim.P3.7

Scer\GAL4^sim.P3.7

Name Synonyms

Secondary FlyBase IDs

References (46)

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