FlyBase Allele Report: Scer\GAL4[per.PK]

General Information

Symbol

Scer\GAL4^per.PK

Species

S. cerevisiae

Name

period promoter construct of Kaneko

FlyBase ID

FBal0060668

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-per.BS}

Also Known As

per-Gal4, period-Gal4, perGAL4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Kaneko, 1997.3.14, Plautz et al., 1997)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GAL4-per.BS}

Cytology

Nature of the lesion

Statement

Reference

4.2kb untranscribed sequence immediately upstream of the transcription start site to +25 site (25 bp into per mRNA) regulates expression of a GAL4 driver.

(Kaneko, 1997.3.14)

4.2kb genomic per fragment regulates expression of a GAL4 driver.

(Plautz et al., 1997)

Allele components

Product class / Tool use(s)

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

photoreceptor neuron

(Kaneko, 1997.3.14)

adult central complex

(Kaneko, 1997.3.14)

adult brain | restricted

(Kaneko, 1997.3.14)

adult pars intercerebralis

(Kaneko, 1997.3.14)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

RP2 motor neuron

(Ruiz et al., 2010)

aCC neuron

(Ruiz et al., 2010)

pCC neuron

(Ruiz et al., 2010)

A1-7 DO1 motor neuron

(Ruiz et al., 2010)

A1-7 DA2 motor neuron

(Ruiz et al., 2010)

U3 neuron

(Ruiz et al., 2010)

EL neuron

(Ruiz et al., 2010)

VUM neuron

Comment: interneuron

(Ruiz et al., 2010)

interneuron of neuroblast MNB

(Ruiz et al., 2010)

UMI interneuron

(Ruiz et al., 2010)

neuroblast NB2-1

Comment: 4 interneurons

(Ruiz et al., 2010)

neuroblast NB4-1

Comment: 2 interneurons

(Ruiz et al., 2010)

embryonic stage 17

H-cell sib

(Wheeler et al., 2006)

VUM interneuron

(Wheeler et al., 2006)

median neuroblast MNB interneuron

(Wheeler et al., 2006)

first instar larval stage

GABAergic neuron | subset

(Babski et al., 2019)

wandering third instar larval stage

period-positive median segmental interneuron

(Kohsaka et al., 2014)

larval ventral nerve cord | restricted

(Kohsaka et al., 2014)

adult stage

antenna

(Kaneko and Hall, 2000)

adult antennal lobe | dorso-lateral | restricted

(Kaneko and Hall, 2000)

adult antennal lobe | restricted | ventro-lateral

(Kaneko and Hall, 2000)

proboscis

(Kaneko and Hall, 2000)

head capsule

(Kaneko and Hall, 2000)

leg

(Kaneko and Hall, 2000)

wing

(Kaneko and Hall, 2000)

adult thorax

(Kaneko and Hall, 2000)

adult abdomen

(Kaneko and Hall, 2000)

testis

(Kaneko and Hall, 2000)

seminal vesicle

(Kaneko and Hall, 2000)

male accessory gland

(Kaneko and Hall, 2000)

ejaculatory bulb

(Kaneko and Hall, 2000)

seminal receptacle

(Kaneko and Hall, 2000)

uterus

(Kaneko and Hall, 2000)

spermatheca

(Kaneko and Hall, 2000)

adult Malpighian tubule

(Kaneko and Hall, 2000)

adult midgut

(Kaneko and Hall, 2000)

pacemaker neuron

(Kaneko and Hall, 2000)

l-LNv neuron

(Liu et al., 2014, Kaneko and Hall, 2000)

s-LNv neuron

Comment: Variably labelled between samples

(Kaneko and Hall, 2000)

DN1 neuron

(Kaneko and Hall, 2000)

DN2 neuron

Comment: Variably labelled between samples

(Kaneko and Hall, 2000)

DN3 neuron

(Kaneko and Hall, 2000)

LNd neuron

Comment: Variably labelled between samples

(Kaneko and Hall, 2000)

posterior optic commissure

(Kaneko and Hall, 2000)

outer medulla

(Kaneko and Hall, 2000)

ocellus | restricted

(Kaneko and Hall, 2000)

retina | restricted

(Kaneko and Hall, 2000)

photoreceptor | subset

(Kaneko and Hall, 2000)

eyelet photoreceptor

(Kaneko and Hall, 2000)

CNS surface associated glial cell | subset

(Kaneko and Hall, 2000)

glial cell of optic chiasma | subset

(Kaneko and Hall, 2000)

epithelial cell of adult heart | subset

(Kaneko and Hall, 2000)

follicle cell | subset

(Kaneko and Hall, 2000)

adult pars intercerebralis | restricted

(Kaneko and Hall, 2000)

cell body rind of the central nervous system | posterior | restricted

(Kaneko and Hall, 2000)

ellipsoid body | subset

(Kaneko and Hall, 2000)

fan-shaped body | subset

(Kaneko and Hall, 2000)

adult mediolateral antennal lobe tract | subset

(Kaneko and Hall, 2000)

adult periesophageal neuropils | restricted

Comment: Cells surrounding the esophageal foramen

(Kaneko and Hall, 2000)

lamina | restricted

(Kaneko and Hall, 2000)

somatic muscle cell of adult thorax | subset

(Kaneko and Hall, 2000)

adult protocerebrum | posterior | subset

(Kaneko and Hall, 2000)

neuron | subset of adult brain

(Chen et al., 2012)

dorsal anterior lateral neuron of the protocerebrum

Comment: faint expression

(Chen et al., 2012)

eye

Comment: faint expression

(Chen et al., 2012)

ellipsoid body

Comment: strong expression

(Chen et al., 2012)

glial cell of adult brain

Comment: faint expression

(Chen et al., 2012)

antennal lobe projection neuron

Comment: medium expression

(Chen et al., 2012)

dorsal fusion commissure

(Kaneko and Hall, 2000)

Additional Information

Statement

Reference

Drives expression in larval and adult pacemaker neurons, including I-LNv and s-LNv neurons, a pair of dorsal anterior lateral neurons of the protocerebrum, and lateral neurons. Also drives expression in a number of midline neurons in late embryos.

(FlyBase Curators, 2017)

Scer\GAL4^per.PK drives expression in a subset of neurons in the ventral nerve cord - VGlut positive period-positive median segmental interneurons and a pair of more lateral neurons. Period-positive median segmental interneurons directly interact with Ecol\lexA^RapGAP1-OK6 expressing motor neurons as shown by a positive GFP reconstitution across synaptic partners (GRASP) signal.

(Kohsaka et al., 2014)

Scer\GAL4^per.PK transcript is expressed in the RP2, aCC, pCC, U1 and U2 neurons. It is also often detected in the U3 and two EL neurons. Lineage analysis shows that Scer\GAL4^per.PK is expressed in the UMI and VUM interneurons and in the interneurons that originate from neuroblasts MNB, NB2-1 and NB4-1.

(Ruiz et al., 2010)

Expression of Scer\GAL4^per.PK is seen in cells located near sensilla on the head capsule, thorax and abdomen of the adult. Expression of Scer\GAL4^per.PK in seen within the abdominal portion of the adult midgut. Expression in the mouth is restricted to the posterior region. Fibres from neurons expressing Scer\GAL4^per.PK enter the distal most layer of the medulla. Expression of Scer\GAL4^per.PK is seen in undefined cells located near to the lobula, and in a pair of cells on either side of the esophagus in the frontal cortex which project along posteriorly along the esophageal foramen. The neurons located dorsolateral to the antennal lobes can be subdivided into two groups: a larger one located dorsomedial to the smaller one. Neurons from both of these groups project to the central complex, with intense arborization in the ellipsoid body and the lateral triangle.

(Kaneko and Hall, 2000)

Putative per-expressing neurons in the adult brain: lateral neurons, dorsal neurons, Hofbauer-Buchner cells. Weak expression in some photoreceptors. Specific neurons in the brain in which endogenous PER has not been detected, such as neurons in central complex and pars intercerebralis.

(Kaneko, 1997.3.14)

Marker for

l-LNv neuron

(Liu et al., 2014)

Reflects expression of

per

(Liu et al., 2014, Chen et al., 2012, Ruiz et al., 2010, Wheeler et al., 2006, Kaneko and Hall, 2000, Kaneko, 1997.3.14)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage, with VGlut^HMS02011

(Kohsaka et al., 2014)

abnormal locomotor behavior | larval stage | conditional, with Npha\HR^{eNpHR2.0.UAS.YFP}

(Kohsaka et al., 2014)

abnormal locomotor behavior | larval stage | heat sensitive, with shi^1.UAS

(Kohsaka et al., 2014)

abnormal locomotor rhythm, with Ctet\tetX^TNT-LC.UAS

(Kaneko et al., 2000)

abnormal locomotor rhythm, with per^UAS.cKa

(Kaneko et al., 2000)

abnormal locomotor rhythm, with Rmic\PDF^UAS.cHa

(Helfrich-Forster et al., 2000)

abnormal memory, with per^{dsRNA.PAS.UAS}

(Chen et al., 2012)

abnormal memory, with Rcom\RT^A-cs2.UAS

(Chen et al., 2012)

abnormal memory | heat sensitive, with shi^1.UAS

(Sakai et al., 2004)

abnormal neurophysiology | larval stage | conditional, with Npha\HR^{eNpHR2.0.UAS.YFP}

(Kohsaka et al., 2014)

abnormal sleep, with Nlg4^GD3083

(Li et al., 2013)

abnormal sleep, with wake^miRNA.UAS.1

(Liu et al., 2014)

abnormal sleep | adult stage, with dco^GD1259

(Wang et al., 2022)

abnormal sleep | adult stage | female, with dyw^HMC04428

(Yang and Edery, 2019)

abnormal sleep | adult stage | female, with dyw^KK107026

(Yang and Edery, 2019)

abnormal sleep | adult stage | male, with dyw^HMC04428

(Yang and Edery, 2019)

abnormal sleep | adult stage | male, with dyw^KK107026

(Yang and Edery, 2019)

abnormal sleep | adult stage | male, with dyw^UAS.cYa

(Yang and Edery, 2019)

increased sleep | adult stage, with dco^GD1259

(Wang et al., 2022)

paralytic | larval stage | conditional, with Crei\ChR2^{H134R.UAS.mCherry}

(Kohsaka et al., 2014)

Phenotype Manifest In

embryonic/larval motor neuron | conditional, with Npha\HR^{eNpHR2.0.UAS.YFP}

(Kohsaka et al., 2014)

larval transverse nerve | conditional, with Npha\HR^{eNpHR2.0.UAS.YFP}

(Kohsaka et al., 2014)

Detailed Description

Statement

Reference

Optogenetic activation (using Crei\ChR2^{H134R.UAS.mCherry} with blue light) of Scer\GAL4^per.PK cells in larvae results in paralysis, which does not occur in controls, for the duration of activation; local stimulation of a region of the ventral nerve cord results in local relaxation of the musculature in the associated larval segments and premature termination of peristalsis.

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^per.PK cells in larvae significantly decreases the speed of peristalsis and locomotion, compared to controls; the duration of segment contraction is significantly increased, compared to controls, with no effect on the extent of muscle contraction (muscle length); when inhibition is restricted to a smaller set of cells using Scer\GAL80^tsh-GAL80, peristalsis occurs at a similar rate to controls; when inhibition is restricted to a smaller set of cells using Scer\GAL80^R70C01, peristalsis is significantly faster than during inhibition of all Scer\GAL4^per.PK cells.

Inhibition (using Npha\HR^UAS.YFP with yellow light) of Scer\GAL4^per.PK cells in larvae significantly decreases the speed of peristalsis, compared to controls; burst duration and pulses per burst of the transverse nerve are significantly increased, compared to control bursts; there is also an increased intersegmental delay in motor neuron bursting, compared to controls.

Inhibition (using VGlut^HMS02011, in combination with a Dicer-2 transgene to enhance RNAi efficiency) of Scer\GAL4^per.PK cells in larvae significantly decreases the speed of peristalsis, compared to controls.

(Kohsaka et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7127

y¹ w^*; P{GAL4-per.BS}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^per.PK

Scer\GAL4^per.PK

Name Synonyms

period promoter construct of Kaneko

(Kaneko, 1997.3.14)

Secondary FlyBase IDs

References (43)

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