FlyBase Allele Report: Scer\GAL4[dimm-929]

General Information

Symbol

Scer\GAL4^dimm-929

Species

S. cerevisiae

Name

FlyBase ID

FBal0060673

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}dimm⁹²⁹

Carried in Construct

Also Known As

C929-GAL4, dimm-GAL4, c929, P{GawB}crc⁹²⁹, c929GAL4

Genomic Maps

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Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hewes et al., 1997, Schaefer and Taghert, 1995)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable element insertion site

2L:21,312,920..21,313,354

(Herrero et al., 2020, Lee et al., 2020, Maurer et al., 2020, Babski et al., 2019, Hill et al., 2019, Moss-Taylor et al., 2019, Schlichting et al., 2019, Yang and Edery, 2019, Bai et al., 2018, Cusumano et al., 2018, Diesner et al., 2018, Feng et al., 2018, Herlo et al., 2018, Li et al., 2018, Valadas et al., 2018, Chng et al., 2017, Kim et al., 2017, Liang et al., 2017, Li et al., 2017, Pathak et al., 2017, Song et al., 2017, Søvik et al., 2017, Petruccelli et al., 2016, Shimada et al., 2016, Bai and Sehgal, 2015, Jeong et al., 2015, Shankar et al., 2015, Cavanaugh et al., 2014, Davis et al., 2014, Kayser et al., 2014, Lamba et al., 2014, Park et al., 2014, Yuan et al., 2014, He et al., 2013, Holst et al., 2013, Li et al., 2013, Losada-Pérez et al., 2013, Jepson et al., 2012, Saleem et al., 2012, Sundram et al., 2012, Yao et al., 2012, Demilly et al., 2011, Hwang and Rulifson, 2011, Jepson et al., 2011, Kahsai and Winther, 2011, Agrawal et al., 2010, Im and Taghert, 2010, Kahsai et al., 2010, Donlea et al., 2009, Grönke et al., 2009, Jansen et al., 2009, Peabody et al., 2009, Shafer and Taghert, 2009, Yew et al., 2009, Hong et al., 2008, Kaplan et al., 2008, Morton et al., 2008, Nässel et al., 2008, Parisky et al., 2008, Park et al., 2008, Peabody et al., 2008, Shang et al., 2008, Zhao et al., 2008, Cerrato et al., 2006, Hewes et al., 2006, Luan et al., 2006, Johnson et al., 2005, Grima et al., 2004, Joshi et al., 2004, Miguel-Aliaga et al., 2004, Osterwalder et al., 2004, Park et al., 2004, Suster et al., 2004, Wegener et al., 2004, Astle et al., 2003, Hewes et al., 2003, Marques et al., 2003, Schroeder et al., 2003, Suster et al., 2003, Seroude et al., 2002, Taghert et al., 2001, Hewes et al., 2000, Hewes et al., 1997, Schaefer and Taghert, 1995)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Insertion components

P{GawB}dimm⁹²⁹

Product class / Tool use(s)

Encoded product / tool

enhancer trap

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

ring gland

(Hewes et al., 2000)

embryonic/larval epidermis | restricted

(Hewes et al., 2000)

embryonic/larval fat body

(Hewes et al., 2000)

neurosecretory neuron of embryonic/larval central nervous system

(Hewes et al., 2000)

embryonic/larval salivary gland

(Hewes et al., 2000)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- third instar larval stage

peritracheal cell

(O'Brien and Taghert, 1998)

third instar larval stage

embryonic/larval corpus cardiacum

(Nässel et al., 2008, Hewes et al., 2003)

embryonic/larval central nervous system | restricted

(Hewes et al., 2006)

embryonic/larval corpus cardiacum | restricted

(Hewes et al., 2006)

apterous-expressing neuron of the lateral cluster of the thorax Ap4

(Hewes et al., 2006, Hewes et al., 2003)

Tvb neuron

(Hewes et al., 2006)

ventral nerve cord | lateral | restricted

(Hewes et al., 2006)

neurosecretory neuron | subset

(O'Brien and Taghert, 1998)

peptidergic neuron | subset of embryonic/larval central nervous system

(Hewes et al., 2003)

ring gland

(Hewes et al., 2003)

dorsal neurohemal organ

(Hewes et al., 2003)

transverse nerve

(Hewes et al., 2003)

embryonic/larval midgut cell | subset

(Hewes et al., 2003)

embryonic/larval peritracheal cell

(Hewes et al., 2003)

lateral Kolbchen lbd

(Hewes et al., 2003)

embryonic/larval fat body

(Hewes et al., 2003)

epithelial cell | subset

(Hewes et al., 2003)

embryonic/larval salivary gland

(Hewes et al., 2003)

neurosecretory neuron of larval pars intercerebralis

(Nässel et al., 2008)

pupal stage

pupal Pdf neuron close to tritocerebrum

(Shafer and Taghert, 2009)

peritracheal cell | subset

(O'Brien and Taghert, 1998)

first instar larval stage -- third instar larval stage

embryonic/larval peritracheal cell | subset

(O'Brien and Taghert, 1998)

first instar larval stage

neuron | subset of embryonic/larval brain

(Hewes et al., 2003)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Saleem et al., 2012)

Additional Information

Statement

Reference

Drives expression in peptidergic neurons.

(FlyBase Curators, 2017)

Scer\GAL4^dimm-929 drives expression in peptidergic neurons in the adult brain.

(Saleem et al., 2012)

The dimm reporter is co-expressed with ITP in ipc-1 and ipc-2a neurons. Three of the ipc-2 neurons do not express the reporter. Coexpression of dimm reported and Tk is also observed in the ipc-1 and ipc-2a neurons.

(Kahsai et al., 2010)

Scer\GAL4^dimm-929 is expressed in the pupal Pdf neuron close to tritocerebrum, which survive in newly eclosed adults but rapidly undergo apoptosis shortly thereafter.

(Shafer and Taghert, 2009)

Scer\GAL4^dimm-929 is expressed in a series of lateral abdominal neurons, and in some insulin-like producing cells (IPCs).

(Hewes et al., 2006)

Co-localisation of Scer\GAL4^dimm-929 and Phm is observed in most of the 200 Scer\GAL4^dimm-929-positive neurons of the central nervous system, corpus cardiacum, 10-20 midgut cells, peritracheal cells and lateral Kolbchen lbd neurons. Co-localisation with FMRFa, Crz, Fur1 or CCAP proteins is detected in subsets or neurons in the central nervous system.

(Hewes et al., 2003)

In larva, expressed in peptidergic neurons of the CNS, intrinsic cells of the ring gland, salivary glands, fat body, patches of the epidermis, the PM peritracheal cells and a few other scattered locations; very restricted expression in the imaginal discs; no detectable expression in the skeletal muscles or the adbominal histoblasts.

(Hewes et al., 2000)

Expression is observed in peritracheal cells prior to second to third instar larval ecdysis.

(O'Brien and Taghert, 1998)

Marker for

peptidergic neuron

(Saleem et al., 2012)

peritracheal cell

(O'Brien and Taghert, 1998)

Reflects expression of

dimm

(Davis et al., 2014, Saleem et al., 2012, Kahsai and Winther, 2011, Shafer and Taghert, 2009, Nässel et al., 2008, Park et al., 2008, Hewes et al., 2006, Hewes et al., 2003, O'Brien and Taghert, 1998)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

behavior defective | male limited, with tll^JF02545

(Davis et al., 2014)

body color defective | adult stage A1, with Sh^{EKO.UAS.GFP(GL)}

(Peabody et al., 2008)

chemical resistant | adult stage, with DopEcR^KK111211

(Petruccelli et al., 2016)

circadian behavior defective | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Parisky et al., 2008)

circadian behavior defective | adult stage | male | heat sensitive, with Scer\GAL80^Pdf.PS, TrpA1^UAS.(B).cKa

(Parisky et al., 2008)

courtship behavior defective | chemical conditional, with Tk^KK112227

(Shankar et al., 2015)

lethal, with Hsap\KCNJ2^UAS.EGFP

(Hong et al., 2008)

lethal, with Nlg4^GD3083

(Li et al., 2013)

lethal | larval stage, with Spn42Da^4.1.UAS

(Osterwalder et al., 2004)

lethal | pupal stage, with Spn42Da^4.1.UAS

(Osterwalder et al., 2004)

lethal - all die before end of larval stage, with Hsap\KCNJ2^UAS.EGFP

(Luan et al., 2006)

lethal - all die before end of larval stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^CCAP.PL

(Luan et al., 2006)

locomotor behavior defective, with Bhal\NaChBac^UAS.EGFP, Ecol\lexA^VP16.Pdf, Scer\FLP1^lexAop.cSa

(Shang et al., 2008)

locomotor behavior defective | heat sensitive, with TrpA1^UAS.(B).cKa

(Shang et al., 2008)

locomotor behavior defective | third instar larval stage, with Ctet\tetX^TNT-LC.UAS

(Suster et al., 2003)

locomotor behavior defective | third instar larval stage, with Hsap\KCNJ2^UAS.EGFP

(Suster et al., 2003)

locomotor rhythm defective | heat sensitive, with lark¹, lark^{dsRNA.UAS.cSb}

(Sundram et al., 2012)

locomotor rhythm defective | heat sensitive, with lark^{dsRNA.UAS.cSb}

(Sundram et al., 2012)

locomotor rhythm defective | heat sensitive, with TrpA1^UAS.(B).cKa

(Cavanaugh et al., 2014)

partially lethal, with DCTN1-p150^Δ.UAS

(Marques et al., 2003)

partially lethal - majority die, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

short lived | conditional, with sNPF^dsRNA.UAS

(Kahsai et al., 2010)

short lived | conditional, with Tk^{dsRNA.UAS.cWa}

(Kahsai et al., 2010)

sleep defective, with Bhal\NaChBac^UAS.EGFP

(Shang et al., 2008)

sleep defective, with Bhal\NaChBac^UAS.EGFP, Scer\GAL80^Pdf.PS

(Shang et al., 2008)

sleep defective, with Ecol\lexA^VP16.Pdf, Scer\FLP1^lexAop.cSa, hid^-FRT.UAS.cSa

(Shang et al., 2008)

sleep defective | adult stage | male, with dyw^HMC04428

(Yang and Edery, 2019)

some die during larval stage, with Ctet\tetX^TNT-LC.UAS

(Joshi et al., 2004)

some die during larval stage, with Hsap\KCNJ2^UAS.EGFP

(Luan et al., 2006)

some die during larval stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^CCAP.PL

(Luan et al., 2006)

some die during pharate adult stage, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

some die during second instar larval stage, with Ctet\tetX^TNT-LC.UAS

(Joshi et al., 2004)

uncoordinated, with DCTN1-p150^Δ.UAS

(Marques et al., 2003)

viable, with LamC^UAS.cSa

(Dialynas et al., 2010)

viable, with LamC^ΔN.UAS.cSa

(Dialynas et al., 2010)

viable, with Mnn1^UAS.cCa

(Cerrato et al., 2006)

Phenotype Manifest In

abdomen | adult stage A1, with Sh^{EKO.UAS.GFP(GL)}

(Peabody et al., 2008)

cephalopharyngeal skeleton, with Ggal\SerpinI1^UAS.cOa

(Osterwalder et al., 2004)

cephalopharyngeal skeleton, with Spn42Da^4.1.UAS

(Osterwalder et al., 2004)

wing, with DCTN1-p150^Δ.UAS

(Marques et al., 2003)

Detailed Description

Statement

Reference

Inhibiting electrical activity in Scer\GAL4^dimm-929-expressing neurons results in reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503): in contrast to wild type, flies do not exhibit courtship defects in the presence of (S,Z,Z)-CH503. Suppressing Scer\GAL4^dimm-929 expression in the ventral nerve cord using Scer\GAL80^tsh-GAL80 does not alter the response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

FMRFa^UAS.cMa/Scer\GAL4^dimm-929 is a suppressor | partially of lethal phenotype of wit^B11/wit^A12

(Marques et al., 2003)

NOT Suppressor of