FlyBase Allele Report: Scer\GAL4[dimm-929]

General Information

Symbol

Scer\GAL4^dimm-929

Species

S. cerevisiae

Name

FlyBase ID

FBal0060673

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}crc⁹²⁹

Carried in Construct

Also Known As

C929-GAL4, dimm-GAL4, c929, P{GawB}crc⁹²⁹, c929GAL4, c929G4

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hewes et al., 1997, Schaefer and Taghert, 1995)

Progenitor genotype

Associated Insertion(s)

P{GawB}crc⁹²⁹

Cytology

Description

The P{GawB}crc⁹²⁹ insertion is within an intron of crc, and complementation analysis indicates that crc function is disrupted by the insertion, producing a hypomorphic allele (see also FBrf0129856). However, the P{GawB} enhancer trap in P{GawB}crc⁹²⁹ does not reflect expression of crc, but instead reflects expression of the dimm gene, a transcription unit that is proximal to the insertion site. Complementation analysis indicates that dimm function is not affected by the insertion.

(Hewes et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:21,312,920..21,313,354

(Dermady et al., 2024, Draper et al., 2024, Rankin et al., 2024, Sukumar et al., 2024, Kasturacharya et al., 2023, Katheder et al., 2023, Lee et al., 2023, Liang et al., 2023, Li et al., 2023, Chaturvedi et al., 2022, Suzuki et al., 2022, Bedont et al., 2021, Fernandez-Chiappe et al., 2021, Inami et al., 2021, Kratschmer et al., 2021, Li et al., 2021, Lund et al., 2021, Polcowñuk et al., 2021, Song et al., 2021, Wat et al., 2021, Herrero et al., 2020, Lee et al., 2020, Liao and Nässel, 2020, Maurer et al., 2020, Babski et al., 2019, Hill et al., 2019, Moss-Taylor et al., 2019, Schlichting et al., 2019, Yang and Edery, 2019, Bai et al., 2018, Cusumano et al., 2018, Diesner et al., 2018, Feng et al., 2018, Herlo et al., 2018, Li et al., 2018, Valadas et al., 2018, Chng et al., 2017, Kim et al., 2017, Liang et al., 2017, Li et al., 2017, Pathak et al., 2017, Seugnet et al., 2017, Song et al., 2017, Søvik et al., 2017, Jayakumar et al., 2016, Petruccelli et al., 2016, Shimada et al., 2016, Tong et al., 2016, Top et al., 2016, Bai and Sehgal, 2015, Jeong et al., 2015, Shankar et al., 2015, Cavanaugh et al., 2014, Davis et al., 2014, Faisal et al., 2014, Kayser et al., 2014, Lamba et al., 2014, Oh et al., 2014, Park et al., 2014, Yuan et al., 2014, He et al., 2013, Holst et al., 2013, Li et al., 2013, Losada-Pérez et al., 2013, Luo et al., 2013, Jepson et al., 2012, Saleem et al., 2012, Sundram et al., 2012, Yao et al., 2012, Demilly et al., 2011, Hwang and Rulifson, 2011, Jepson et al., 2011, Kahsai and Winther, 2011, Agrawal et al., 2010, Im and Taghert, 2010, Kahsai et al., 2010, Donlea et al., 2009, Grönke et al., 2009, Jansen et al., 2009, Peabody et al., 2009, Shafer and Taghert, 2009, Yew et al., 2009, Hong et al., 2008, Kaplan et al., 2008, Morton et al., 2008, Nässel et al., 2008, Parisky et al., 2008, Park et al., 2008, Peabody et al., 2008, Shang et al., 2008, Zhao et al., 2008, Cerrato et al., 2006, Hewes et al., 2006, Luan et al., 2006, Johnson et al., 2005, Grima et al., 2004, Joshi et al., 2004, Miguel-Aliaga et al., 2004, Osterwalder et al., 2004, Park et al., 2004, Suster et al., 2004, Wegener et al., 2004, Astle et al., 2003, Hewes et al., 2003, Marques et al., 2003, Schroeder et al., 2003, Suster et al., 2003, Seroude et al., 2002, Taghert et al., 2001, Hewes et al., 2000, Hewes et al., 1997, Schaefer and Taghert, 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

ring gland

(Hewes et al., 2000)

embryonic/larval epidermis | restricted

(Hewes et al., 2000)

embryonic/larval fat body

(Hewes et al., 2000)

neurosecretory neuron of larval central nervous system

(Hewes et al., 2000)

embryonic/larval salivary gland

(Hewes et al., 2000)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

neuron | subset of larval brain

(Hewes et al., 2003)

first instar larval stage -- third instar larval stage

embryonic/larval peritracheal cell | subset

(O'Brien and Taghert, 1998)

second instar larval stage -- third instar larval stage

peritracheal cell

(O'Brien and Taghert, 1998)

third instar larval stage

embryonic/larval corpus cardiacum

(Nässel et al., 2008, Hewes et al., 2003)

larval central nervous system | restricted

(Hewes et al., 2006)

embryonic/larval corpus cardiacum | restricted

(Hewes et al., 2006)

apterous-expressing neuron of the lateral cluster of the thorax Ap4

(Hewes et al., 2006, Hewes et al., 2003)

apterous-expressing neuron of the lateral cluster of the thorax Ap1

(Hewes et al., 2006)

larval ventral nerve cord | lateral | restricted

(Hewes et al., 2006)

neurosecretory neuron | subset

(O'Brien and Taghert, 1998)

peptidergic neuron | subset of larval central nervous system

(Hewes et al., 2003)

ring gland

(Hewes et al., 2003)

larval thoracic perisympathetic organ

(Hewes et al., 2003)

larval transverse nerve

(Hewes et al., 2003)

embryonic/larval midgut cell | subset

(Hewes et al., 2003)

embryonic/larval peritracheal cell

(Hewes et al., 2003)

lateral Kolbchen lbd

(Hewes et al., 2003)

embryonic/larval fat body

(Hewes et al., 2003)

epithelial cell | subset

(Hewes et al., 2003)

embryonic/larval salivary gland

(Hewes et al., 2003)

neurosecretory neuron of larval pars intercerebralis

(Nässel et al., 2008)

pupal stage

pupal Pdf neuron close to tritocerebrum

(Shafer and Taghert, 2009)

peritracheal cell | subset

(O'Brien and Taghert, 1998)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Saleem et al., 2012)

Additional Information

Statement

Reference

Drives expression in peptidergic neurons.

(FlyBase Curators, 2017)

ScerGAL4^dimm-929 drives expression in peptidergic neurons in the adult brain.

(Saleem et al., 2012)

The dimm reporter is co-expressed with ITP in ipc-1 and ipc-2a neurons. Three of the ipc-2 neurons do not express the reporter. Coexpression of dimm reported and Tk is also observed in the ipc-1 and ipc-2a neurons.

(Kahsai et al., 2010)

ScerGAL4^dimm-929 is expressed in the pupal Pdf neuron close to tritocerebrum, which survive in newly eclosed adults but rapidly undergo apoptosis shortly thereafter.

(Shafer and Taghert, 2009)

ScerGAL4^dimm-929 is expressed in a series of lateral abdominal neurons, and in some insulin-like producing cells (IPCs).

(Hewes et al., 2006)

Co-localisation of ScerGAL4^dimm-929 and Phm is observed in most of the 200 ScerGAL4^dimm-929-positive neurons of the central nervous system, corpus cardiacum, 10-20 midgut cells, peritracheal cells and lateral Kolbchen lbd neurons. Co-localisation with FMRFa, Crz, Fur1 or CCAP proteins is detected in subsets or neurons in the central nervous system.

(Hewes et al., 2003)

In larva, expressed in peptidergic neurons of the CNS, intrinsic cells of the ring gland, salivary glands, fat body, patches of the epidermis, the PM peritracheal cells and a few other scattered locations; very restricted expression in the imaginal discs; no detectable expression in the skeletal muscles or the adbominal histoblasts.

(Hewes et al., 2000)

Expression is observed in peritracheal cells prior to second to third instar larval ecdysis.

(O'Brien and Taghert, 1998)

Marker for

peptidergic neuron

(Saleem et al., 2012)

peritracheal cell

(O'Brien and Taghert, 1998)

Reflects expression of

dimm

(Davis et al., 2014, Saleem et al., 2012, Kahsai and Winther, 2011, Shafer and Taghert, 2009, Nässel et al., 2008, Park et al., 2008, Hewes et al., 2006, Hewes et al., 2003, O'Brien and Taghert, 1998)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | male limited, with tll^JF02545

(Davis et al., 2014)

abnormal body color | adult stage A1, with Sh^{EKO.UAS.GFP(GL)}

(Peabody et al., 2008)

abnormal circadian behavior | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Parisky et al., 2008)

abnormal circadian behavior | adult stage | male | heat sensitive, with Scer\GAL80^Pdf.PS, TrpA1^UAS.(B).cKa

(Parisky et al., 2008)

abnormal circadian rhythm | adult stage, with Pdf^GD299

(Herrero et al., 2020)

abnormal circadian rhythm | adult stage, with Pdf^GD299, Scer\GAL4^sLNvs

(Herrero et al., 2020)

abnormal courtship behavior | chemical conditional, with Tk^KK112227

(Shankar et al., 2015)

abnormal developmental rate, with Bhal\NaChBac^UAS.EGFP

(Dermady et al., 2024)

abnormal locomotor behavior, with Bhal\NaChBac^UAS.EGFP, Ecol\lexA^VP16.Pdf, Scer\FLP1^lexAop.cSa

(Shang et al., 2008)

abnormal locomotor behavior | heat sensitive, with TrpA1^UAS.(B).cKa

(Shang et al., 2008)

abnormal locomotor behavior | third instar larval stage, with Ctet\tetX^TNT-LC.UAS

(Suster et al., 2003)

abnormal locomotor behavior | third instar larval stage, with Hsap\KCNJ2^UAS.EGFP

(Suster et al., 2003)

abnormal locomotor rhythm | heat sensitive, with lark¹, lark^RNAi.UAS.cSb

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with lark^RNAi.UAS.cSb

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with TrpA1^UAS.(B).cKa

(Cavanaugh et al., 2014)

abnormal neuroanatomy, with mir-210^UAS.cCa

(Cusumano et al., 2018)

abnormal neuroanatomy | adult stage, with Pdf^GD299

(Herrero et al., 2020)

abnormal neuroanatomy | adult stage, with Pdf^GD299, Scer\GAL4^sLNvs

(Herrero et al., 2020)

abnormal sleep, with Bhal\NaChBac^UAS.EGFP

(Shang et al., 2008)

abnormal sleep, with Bhal\NaChBac^UAS.EGFP, Scer\GAL80^Pdf.PS

(Shang et al., 2008)

abnormal sleep, with Ecol\lexA^VP16.Pdf, Scer\FLP1^lexAop.cSa, hid^-FRT.UAS.cSa

(Shang et al., 2008)

abnormal sleep | adult stage, with wake^RNAi.UAS.2

(Chaturvedi et al., 2022)

abnormal sleep | adult stage | male, with dyw^HMC04428

(Yang and Edery, 2019)

chemical resistant | adult stage, with DopEcR^KK111211

(Petruccelli et al., 2016)

decreased sleep | adult stage, with wake^RNAi.UAS.2

(Chaturvedi et al., 2022)

lethal, with Hsap\KCNJ2^UAS.EGFP

(Hong et al., 2008)

lethal, with Nlg4^GD3083

(Li et al., 2013)

lethal | larval stage, with Spn42Da^4.1.UAS

(Osterwalder et al., 2004)

lethal | pupal stage, with Spn42Da^4.1.UAS

(Osterwalder et al., 2004)

lethal - all die before end of larval stage, with Hsap\KCNJ2^UAS.EGFP

(Luan et al., 2006)

lethal - all die before end of larval stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^CCAP.PL

(Luan et al., 2006)

partially lethal, with DCTN1-p150^Δ.UAS

(Marques et al., 2003)

partially lethal - majority die, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

short lived | conditional, with sNPF^RNAi.UAS

(Kahsai et al., 2010)

short lived | conditional, with Tk^RNAi.UAS.cWa

(Kahsai et al., 2010)

some die during larval stage, with Ctet\tetX^TNT-LC.UAS

(Joshi et al., 2004)

some die during larval stage, with Hsap\KCNJ2^UAS.EGFP

(Luan et al., 2006)

some die during larval stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^CCAP.PL

(Luan et al., 2006)

some die during pharate adult stage, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

some die during second instar larval stage, with Ctet\tetX^TNT-LC.UAS

(Joshi et al., 2004)

uncoordinated, with DCTN1-p150^Δ.UAS

(Marques et al., 2003)

viable, with LamC^UAS.cSa

(Dialynas et al., 2010)

viable, with LamC^ΔN.UAS.cSa

(Dialynas et al., 2010)

viable, with Mnn1^UAS.cCa

(Cerrato et al., 2006)

visible | adult stage, with Rab2^KK107630

(Lund et al., 2021)

Phenotype Manifest In

abdomen | adult stage A1, with Sh^{EKO.UAS.GFP(GL)}

(Peabody et al., 2008)

adult s-LNv neuron | adult stage, with Pdf^GD299

(Herrero et al., 2020)

adult s-LNv neuron | adult stage, with Pdf^GD299, Scer\GAL4^sLNvs

(Herrero et al., 2020)

cephalopharyngeal skeleton, with Ggal\SerpinI1^UAS.cOa

(Osterwalder et al., 2004)

cephalopharyngeal skeleton, with Spn42Da^4.1.UAS

(Osterwalder et al., 2004)

l-LNv neuron, with mir-210^UAS.cCa

(Cusumano et al., 2018)

neuronal cell body, with mir-210^UAS.cCa

(Cusumano et al., 2018)

neuron projection, with mir-210^UAS.cCa

(Cusumano et al., 2018)

wing, with DCTN1-p150^Δ.UAS

(Marques et al., 2003)

wing, with Rab2^KK107630

(Lund et al., 2021)

Detailed Description

Statement

Reference

Inhibiting electrical activity in Scer\GAL4^dimm-929-expressing neurons results in reduced sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503): in contrast to wild type, flies do not exhibit courtship defects in the presence of (S,Z,Z)-CH503. Suppressing Scer\GAL4^dimm-929 expression in the ventral nerve cord using Scer\GAL80^tsh-GAL80 does not alter the response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^dimm-929, wake^RNAi.UAS.2 has decreased sleep | adult stage phenotype, non-enhanceable by Gat^33-1

(Chaturvedi et al., 2022)

Scer\GAL4^dimm-929, wake^RNAi.UAS.2 has abnormal sleep | adult stage phenotype, non-enhanceable by Gat^33-1