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General Information
Symbol
Scer\GAL4VP16.nos.UTR
Species
S. cerevisiae
Name
FlyBase ID
FBal0060680
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
nos-GAL4, nanos-Gal4, nanos-Gal4-VP16, Nanos-Gal4:VP16, nos-gal4-vp16, MTD-Gal4, nosGal4, nanos-Gal4::VP16, nanos-GAL4VP16, nosGal4VP16
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
nos coding sequences within nosgnosb are replaced with sequences encoding the Tag:FLAG epitope and a GAL4::VP16 driver. The construct contains approximately 700bp of the nos promoter, the nos 5' and 3' UTRs and approximately 500bp of genomic sequences 3' of nos.
Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference
Drives expression in female germline stem cells at all developmental stages.
On the basis of expression of P{UAS-lacZ.B} target construct, early expression is detected in posterior somatic cells, but not in pole cells. Posterior expression exhibits a posterior-to-anterior concentration gradient. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.
In the early embryo, Scer\GAL4nos.UTR.T:Hsim\VP16 RNA detected in pole cells and subsequently in germ cells. However, expression ofthe GAL4::VP16-responsive target construct, P{UAS-lacZ.B}, differs. Early expression is detected in posterior somatic cells, but not in pole cells. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.
Expression is observed in female germline stem cells at all stages and at a high level..
 
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
actin filament & nurse cell, with Dcp-1ΔN.UASp
egg & nuclear chromosome, with Pp2B-14Dact.UASp
embryonic/first instar larval cuticle & embryonic head, with osk3'UTR.UASp
meiosis & nuclear chromosome, with AxsD.UASp
meiosis & nuclear chromosome & spindle, with AxsD.UASp
nurse cell & actin filament, with MoeTA.UAS.EGFP
nurse cell & actin filament, with MoeTD.UAS.EGFP
oocyte & actin filament | ectopic, with MoeTA.UAS.EGFP
oocyte & nuclear chromatin, with Pp2B-14Dact.UASp
oocyte & nucleus, with Pp2B-14Dact.UASp
spindle microtubule & oocyte, with subATP.UASp.Tag:HA
spindle microtubule & oocyte, with subCT.UASp.EGFP
Detailed Description
Statement
Reference
Expression does not affect germline development.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Scer\GAL4VP16.nos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by Hrb27C[+]/Hrb27Ck02814
Scer\GAL4VP16.nos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by Hrb27C[+]/Hrb27Ck16203
Scer\GAL4VP16.nos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by bru1QB/aret[+]
Scer\GAL4VP16.nos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by Hrb27C[+]/Hrb27C02647
Scer\GAL4VP16.nos.UTR, kek1UASp.cGa has egg phenotype, enhanceable by Egfr[+]/Egfrt1
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference
Scer\GAL4VP16.nos.UTR/Hsap\DYNLL1UAS.Tag:MYC is a suppressor of actin filament bundle & nucleus phenotype of ctpexc39
Scer\GAL4VP16.nos.UTR/Hsap\DYNLL1UAS.Tag:MYC is a suppressor of actin filament bundle & nucleus phenotype of ctpins1
Scer\GAL4VP16.nos.UTR/Hsap\DYNLL1UAS.Tag:MYC is a suppressor of actin filament bundle & nucleus phenotype of ctpDIIA82
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescues
Partially rescues
Fails to rescue
Comments
Images (0)
Mutant
Wild-type
Stocks (12)
Notes on Origin
Discoverer
Comments
Comments
Used to drive αTub84BScer\UAS.P\T.T:Avic\GFP-S65C in the early embryo to use as a marker.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
GAL4::VP16nos.UTR
Gal4nanos-VP16
Gal4pnos-VP16
Scer\GAL4VP16.nos.UTR
Scer\GAL4nos.UTR.T:Hsim\VP16
Name Synonyms
Secondary FlyBase IDs
    References (482)