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General Information
Symbol
Dmel\kayUAS.cEa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Eresh
FlyBase ID
FBal0060698
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-fos, UAS-Dfos, UAS-FosWT
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of full-length kay.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval eye-antennal disc clones expressing kayScer\UAS.cEa under the control of Scer\GAL4Act.PU do not exhibit size defects or differences in number or morphology of clones, as compared to controls.

Expression of kayScer\UAS.cEa in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) has no significant effect on RP2 dendrite volume.

Expression of kayScer\UAS.cEa under the control of Scer\GAL4pnr-MD237 results in a weak cleft thorax phenotype, while expression under either Scer\GAL4ey.PH or Scer\GAL4how-24B does not result in a visible phenotype.

Flies expressing kayScer\UAS.cEa under the control of Scer\GAL4αTub84B.PL are semi-viable at 18 and 25oC.

Expression of kayScer\UAS.cEa under the control of Scer\GAL4elav-C155 does not affect EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction. mEJC amplitude is not significantly altered.

Flies expressing kayScer\UAS.cEa under the control of Scer\GAL4tim.Scer\UAS have wild-type locomotor activity rhythms.

Causes no effect on copper cell development, when expression is driven by Scer\GAL448Y.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Larval eye-antennal disc clones co-expressing kayScer\UAS.cEa and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU exhibit hyperplasia but no invasive growth, as compared to controls, and pupae containing these clones die during the P4 or P5 stage.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4D42 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) results in a significant increase in total dendritic length and in the number of dendritic branches in MN5 motorneurons. The length and radii of individual dendrite segments and dendritic territory borders are unaffected.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4103.3 results in a significant increase in total dendritic length and in the number of dendritic branches in MN5 motorneurons. The length and radii of individual dendrite segments and dendritic territory borders are unaffected.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4futsch-C380 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) results in a significant decrease in total dendritic length and in the number of dendritic branches in MN5 motorneurons. The length and radii of individual dendrite segments are unaffected. The dendrites do not reach the normal neuropil boundaries and appear less ramified with several parts missing. The most severe defects are in the proximal-posterior region.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4gfA-3295 results in a significant reduction in the number of adult MN5 dendrites.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4futsch-C380 results in significantly fewer dendritic branches in the MN5 motorneurons. If expression is delayed past pupal stage P6 by using the temperature-sensitive Scer\GAL80ts.αTub84B allele, then no obvious dendritic defects are seen.

The mutant eye phenotype caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is is completely suppressed by co-expression of kayScer\UAS.cEa.

Co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa pan-neurally under the control of Scer\GAL4elav-C155 positively regulates synaptic strength.

Co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 increases synaptic size (measured as the number of synaptic boutons) compared to wild-type controls.

Co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa rescues the small eye phenotype seen upon expression of kayFbz.Scer\UAS in the eye disc using Scer\GAL4ey.3.5.Exel.

Co-expression of kayScer\UAS.cEa and sggA81T.Scer\UAS pan-neurally under the control of Scer\GAL4elav-C155 strongly reduces EJC amplitude.

Expression of sggEY02862 suppresses the reduced EJC amplitude found upon expression of kayScer\UAS.cEa and JraScer\UAS.cEa pan-neurally under the control of Scer\GAL4elav-C155.

Co-expression of cnkEY06675 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of Fkbp13EY02405 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of Pde8EY10143 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of sggEY02862 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of SdcEY04602 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, significantly reduces synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of Sema-2aEY08184 in flies already expressing JraScer\UAS.cEa and kayScer\UAS.cEa, all in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155, does not affect synaptic growth compared to JraScer\UAS.cEa and kayScer\UAS.cEa over-expression flies.

Co-expression of both kayScer\UAS.cEa and JraScer\UAS.cEa in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) results in a significant increase in RP2 dendrite volume compared to controls.

Cultured motor neurons co-expressing both kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4futsch-C380 (in the presence of Scer\GAL80Cha.PK to suppress expression in cholinergic neurons) show a significant increase in both neurite length and branch number compared to controls.

Simultaneous co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa under the control of Scer\GAL4elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

Co-expression of kayScer\UAS.cEa has no effect on the symmetrical ommatidia phenotype caused by expression of pntP2.Scer\UAS under the control of Scer\GAL4hs.2sev.

Coexpression of kayScer\UAS.cEa and Mnn1Scer\UAS.cCa under the control of Scer\GAL4pnr-MD237 results in an enhancement of the cleft thorax phenotypes seen when either is expressed alone.

Expression of both kayScer\UAS.cEa and JraScer\UAS.cEa under the control of Scer\GAL4elav-C155 results in a 30% increase in EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction. mEJC amplitude is not significantly altered. Expression of CrebB-17Ab.hs does not change the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on bouton number, but significantly inhibits the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on EJC amplitude (synaptic strength); the triple mutant flies have an EJC amplitude 108% that of control flies. Expression of dncScer\UAS.cCa under the control of Scer\GAL4elav-C155 does not change the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on bouton number, but significantly inhibits the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on EJC amplitude (synaptic strength); the triple mutant flies have an EJC amplitude 83% that of control flies. Expression of pucScer\UAS.cMa under the control of Scer\GAL4elav-C155 suppresses the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on bouton number and EJC amplitude (synaptic strength) at the larval neuromuscular junction.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Scer\GAL4pnr-MD237-mediated expression in kay1/kay2 transheterozygous embryos fails to rescue the dorsal-open phenotype. Scer\GAL469B-mediated expression rescues the embryonic phenotype of kay1/kay2 and partially rescues mutants to pharate adults.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
FraUAS.cEa
kayScer\UAS.cEa
kayUAS.cEa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Eresh
Secondary FlyBase IDs
    References (21)