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General Information
Symbol
Dmel\kayFbz.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0060700
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Fbz, UAS-fosDN, UAS-kayDN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of amino acids 252-337 (the bZIP region) of kay.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Overexpression of kayFbz.Scer\UAS in combination with Scer\GAL4Scer\UAS.cHa driven by Scer\GAL4Tab2-201Y does not affect axon pruning.
Neuronal expression of kayFbz.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 or Scer\GAL4nSyb.PS does not affect neuromuscular junction growth in a wild-type genetic background.
ddaC neurons show robust dendrite regeneration after dendrite removal in animals expressing kayFbz.Scer\UAS under the control of Scer\GAL4477. ddaE neurons show robust dendrite regeneration after dendrite removal in animals expressing kayFbz.Scer\UAS under the control of Scer\GAL4221.
Expression of kayFbz.Scer\UAS under the control of Scer\GAL4BG380 has no effect on maximum bouton diameter at the larval neuromuscular junction.
Expression of 2 copies of kayFbz.Scer\UAS using Scer\GAL4ey-OK107 results in axon overextension.
Axonal sprouting of the injured axon after a crush injury to the segmental nerve in third instar larvae is significantly inhibited in animals expressing kayFbz.Scer\UAS under the control of Scer\GAL4tey-5053A.
Expression of kayFbz.Scer\UAS in the eye disc using Scer\GAL4ey.3.5.Exel generates a small eye phenotype. Expression of kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 results in synapses that are approximately 67-69% of the size of control synapses (measured by the number of synaptic boutons). Expression of kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 reduces transmitter release.
Expression of kayFbz.Scer\UAS in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) results in a significant decrease in RP2 dendrite volume compared to controls. Cultured motor neurons expressing kayFbz.Scer\UAS under the control of Scer\GAL4futsch-C380 (in the presence of Scer\GAL80Cha.PK to suppress expression in cholinergic neurons) show a significant decrease in both neurite length and branch number compared to controls.
Expression of kayFbz.Scer\UAS, under the control of Scer\GAL4BG380 reduces the bouton number and branching and increasing the intensity of staining for synaptic vesicle markers at the synapse, compared to wild-type.
Expression of kayFbz.Scer\UAS under the control of Scer\GAL4elav-C155 results in an approximately 30% reduction in EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction. mEJC amplitude is not significantly altered.
When kayFbz.Scer\UAS is driven by Scer\GAL4dpp.blk1 loss of the anterior crossvein in the wing is seen. When driven by Scer\GAL4en-e16E loss of the anterior crossvein and partial loss of the longitudinal vein V is seen. When driven by Scer\GAL4sd-SG29.1 longitudinal vein V and parts of veins II and IV are missing. When kayFbz.Scer\UAS is expressed under the control of Scer\GAL4dpp.blk1 in a kay1 heterozygote, there is an almost complete loss of longitudinal vein III. When kayFbz.Scer\UAS is expressed under the control of Scer\GAL4en-e16E in a kay1 heterozygote a more complete loss of longitudinal vein V is seen. When kayFbz.Scer\UAS is expressed under the control of Scer\GAL4sd-SG29.1 in a kay1 heterozygote all wing vein is strongly reduced. When kayFbz.Scer\UAS is driven by Scer\GAL4h-1J3 or Scer\GAL4hs.2sev, eyes lack photoreceptor cells and have misoriented ommatidia.
Scer\GAL4-mediated expression in the eye imaginal disc interferes with proper eye development. Expression in the embryonic endoderm interferes with copper cell development.
When expression is driven by Scer\GAL448Y the number of copper cells is strikingly (though variably) reduced. In 5% of the larave copper cells were missing all together. Interstitial cells are reduced in number, and large flat cells increased in number. When expression is driven by Scer\GAL4hs.PB, with heat shocks at the second larval stage, numbers of copper cells are greatly reduced, or completely suppressed.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference
Scer\GAL432B/kayFbz.UAS is a suppressor of wing | ectopic phenotype of EgfrE3
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Neuronal expression of kayFbz.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 is sufficient to completely alleviate the synaptic overgrowth in Rab81/Rab8B229 mutants. Neuronal expression of kayFbz.Scer\UAS under the control of Scer\GAL4nSyb.PS is sufficient to completely alleviate the synaptic overgrowth in Rab81/Rab8B229 mutants.
Expression of kayFbz.Scer\UAS in enteroblasts under the control of Scer\GAL4Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a6 mutant flies.
The synaptic overgrowth phenotype (increase in bouton number) seen at the neuromuscular junction in larvae derived from homozygous SkpAGD65 females is partially suppressed by expression of kayFbz.Scer\UAS under the control of Scer\GAL4elav.PU.
Expression of kayFbz.Scer\UAS under the control of Scer\GAL4elav.PU suppresses the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.
Expression of kayFbz.Scer\UAS in motor neurons under the control of Scer\GAL4unspecified suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in comt6 Ca-P60AKum170 larvae. Co-expression of kayFbz.Scer\UAS suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in larvae expressing Ras85DV12.S35.Scer\UAS in the motor neurons under the control of Scer\GAL4unspecified. Co-expression of kayFbz.Scer\UAS does not suppress the eye tumour phenotype caused by expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel.
Co-expression of kayFbz.Scer\UAS and JraJbz.Scer\UAS results in axon degeneration phenotypes. Co-expression of one copy of kayFbz.Scer\UAS strongly enhances the axonal defects seen in Scer\GAL4ey-OK107, bskGD10555 animals.
Expression of Cnx99AEP3522 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of SdcEY04602 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of Sema-2aEY08184 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of cnkEY06675 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of Pde8EY10143 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of Fkbp13EY02405 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of styEY11239 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of pigeonEY06835 suppresses the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of spagEY11196 suppresses the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of lbmEY02692 suppresses the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of MbsEP3727 suppresses the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Expression of Pvf1EP1624 suppresses the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Co-expression of sggA81T.Scer\UAS and kayFbz.Scer\UAS in post-mitotic neurons under the control of Scer\GAL4elav-C155 significantly reduces EJC amplitude, beyond either transgene alone. Co-expression of JraScer\UAS.cEa and kayScer\UAS.cEa rescues the small eye phenotype seen upon expression of kayFbz.Scer\UAS in the eye disc using Scer\GAL4ey.3.5.Exel. Expression of pigeonEY06835 enhances the small eye phenotype associated with kayFbz.Scer\UAS, when both are expressed under the control of Scer\GAL4ey.3.5.Exel. Co-expression of pigeonEY06835 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 partially suppresses the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of lbmEY02692 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 partially suppresses the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of Cnx99AEP3522 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 partially suppresses the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of styEY11239 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 partially suppresses the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of cnkEY06675 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 enhances the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of Fkbp13EY02405 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 enhances the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of Pde8EY10143 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 enhances the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of sggEY02862 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 enhances the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of SdcEY04602 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 has no effect on the small synapse phenotype found in kayFbz.Scer\UAS mutants. Co-expression of Sema-2aEY08184 with kayFbz.Scer\UAS in differentiated post-mitotic neurons under the control of Scer\GAL4elav-C155 has no effect on the small synapse phenotype found in kayFbz.Scer\UAS mutants.
Expression of kayFbz.Scer\UAS, under the control of Scer\GAL4BG380, confers dramatic suppression of the hiwND8 synaptic phenotype, reducing bouton number and branching (42%) and increasing the intensity of staining for synaptic vesicle markers at the synapse. Co-expression of kayFbz.Scer\UAS, under the control of Scer\GAL4BG380, with wndScer\UAS.cCa in neurons suppresses the wnd gain-of-function phenotype, leading to a 38% reduction in synaptic bouton number, a 52% reduction in synaptic branching, a 54% increase in bouton size, and a 3.8-fold increase in the intensity of staining of synaptic vesicle markers.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
Protein acts as a dominant negative as the bZIP domain is able to dimerize and bind DNA without being able to stimulate transcription.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
FraFbz.UAS
kayFbz.Scer\UAS
kayFbz.UAS
Name Synonyms
Secondary FlyBase IDs
    References (26)