FlyBase Allele Report: Dmel\rho[UAS.cdCa]

Expressing rho^UAS.cdCa under the control of Scer\GAL4^twi.2PE results in the near absence of embryonic ostial cardioblasts, despite of no significant effect in the overall number of cardioblasts.

Expression under the control of Scer\GAL4^how-24B leads to a small but significant decrease in the number of embryonic ostial cardioblasts and a converse small but significant increase in the number of generic embryonic cardioblasts, leading to no change in the overall number of embryonic cardioblasts, as compared to controls.

Expression under the control of Scer\GAL4^pnr-MD237 does not significantly affect the numbers of embryonic cardioblasts, as compared to controls.

Expression under the double control Scer\GAL4^tin.cBa and Scer\GAL4^tin.CΔ4 leads to a severe increase in the overall number of embryonic cardioblasts, namely of generic cardioblasts but not of embryonic ostial cardioblasts. This expression also leads to a significant increase in the number of odd and Tin-positive pericardial cells, although the number of even pericardial cells seems unaffected, as compared to controls.

(Schwarz et al., 2018)

Ectopic expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^GMR.PU results in supernumerary cells of all types in the pupal retina.

(Martín-Bermudo et al., 2015)

Expression via Scer\GAL4^fkh.PH results in salivary glands positioned too anteriorly with no obvious distinction in shape between duct and secretory cells and no common duct-like structure formed at stage 15. At earlier stages during the invagination process, aberrantly shaped lumena are observed, but most prominently a large bulge of eyg-positive ectopic cells seem to arise between the already invaginated secretory gland portions at the position where the individual ducts would normally form.

Expression via Scer\GAL4^arm.PU leads to embryos with varying degrees of affected morphology (in many cases head involution fails) and the general appearance of the epidermis is less organized compared to wild type, although epithelial integrity/polarity appears unperturbed. Only very short glands invaginate into the embryo, and a large bulge of eyg-positive cells is found at the surface of the embryo between the two invagination sides.

Whereas control salivary gland placode cells at stages 12-13 do not undergo division, Scer\GAL4^fkh.PH rho^{Scer\UAS.cdCa} placode cells are actively dividing.

(Maybeck and Röper, 2009)

Overexpression of rho^{Scer\UAS.cdCa}, under the control of Scer\GAL4^salm-459.2, gives rise to an invagination phenotype. Two initial invagination sites appear in the placode, and cells from the dorsal side do not rotate, leading to the formation of a double arch at the early stage 11 placode.

(Brodu and Casanova, 2006)

When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^dpp.blk1 no effect is seen on wing disc growth.

(Garoia et al., 2005)

In rho^{Scer\UAS.cdCa}; Scer\GAL4^en-e16E embryos, induction of larval oenocyte precursors begins at the normal time (during stage 9) and is followed by two waves of delamination with the number of cells (average 3 per wave from each oenocyte primordium) and timing of delamination as in wild-type. However, in these embryos, additional (heterochronic) waves of induction and delamination occur after these two waves (throughout stage 12), resulting in increased numbers of mature oenocytes in stage 16 embryos. Oenocyte clusters in these embryos show a multi-modal distribution of cell numbers with peaks corresponding to multiples of 3 (12, 15, 18 and 21 compared to the wild-type average of 6 resulting from 2 delamination cycles), suggesting there are up to 4 additional waves of delamination. The additional pulses of larval oenocyte precursor delamination occur with the same periodicity as the first two phases in wild-type.

(Brodu et al., 2004)

When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^sim.PS an increase is see in the number of proliferating midbrain cells.

(Page, 2003)

Expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^en-e16E or Scer\GAL4^ato.3.6 results in the formation of ectopic oenocytes in the T1-T3 segments (they are normally only found in segments A1-A7).

(Brodu et al., 2002)

rho^{Scer\UAS.cdCa}; Scer\GAL4^Bx-MS1096 flies have blistered wings with large amounts of ectopic vein material.

(Urban et al., 2002)

The number of chordotonal organs in the lateral cluster is increased from 5 to 6 in embryos expressing rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^en-e16E. In stage 11 embryos, the oenocyte precursor whorl is enlarged and by stage 16 oenocyte clusters containing 17-27 cells are seen.

(Elstob et al., 2001)

Expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^Bx-MS1096 results in small, heavily pigmented wings with excess vein material.

(Lee et al., 2001)

Expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^GMR.PF disrupts eye development leading to a rough eye phenotype. At the cellular level excess cone and pigment cell recruitment is seen, excess photoreceptors are also sometimes seen. When rho^{Scer\UAS.cdCa} is expressed under the control of Scer\GAL4^Bx-MS1096, leads to small, pigmented, blistered wings: all cells in the wing are concerted to vein cells. When rho^{Scer\UAS.cdCa} is expressed under the control of Scer\GAL4^CY2, embryos have a dorsalised phenotype.

(Wasserman et al., 2000)

Scer\GAL4^c179-mediated expression of HLHmβ^{Scer\UAS.cdCa} in the wing causes deletion of most veins and Scer\GAL4^salm-459.2-mediated expression causes truncation of vein LIV. Expression of rho^{Scer\UAS.cdCa} rescues vein loss and results in thicker veins.

(de Celis et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^MD-638, rho^UAS.cdCa has visible | adult stage phenotype, enhanceable by tay^RNAi.UAS/Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Enhancer of

Statement

Reference

rho^UAS.cdCa/Scer\GAL4^MD-638 is an enhancer of visible | adult stage phenotype of Scer\GAL4^MD-638, tay^RNAi.UAS

(Molnar and de Celis, 2013)

rho^UAS.cdCa/Scer\GAL4^dpp.blk1 is an enhancer of increased cell number phenotype of ft^G-rv

(Garoia et al., 2005)

rho^UAS.cdCa/Scer\GAL4^Bx-MS1096 is an enhancer of increased cell number | somatic clone phenotype of ft^k07918

(Garoia et al., 2005)

NOT Enhancer of

Statement

Reference

rho^UAS.cdCa/Scer\GAL4^Bx-MS1096 is a non-enhancer of abnormal planar polarity | somatic clone phenotype of ft^k07918

(Garoia et al., 2005)

Suppressor of

Statement

Reference

rho^UAS.cdCa/Scer\GAL4^hh.PU is a suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

rho^UAS.cdCa/Scer\GAL4^Act.PU is a suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Rbf^15aΔ

(Sheng et al., 2016)

NOT Suppressor of

Statement

Reference

rho^UAS.cdCa/Scer\GAL4^Act.PU is a non-suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

rho^UAS.cdCa/Scer\GAL4^Bx-MS1096 is a non-suppressor of abnormal planar polarity | somatic clone phenotype of ft^k07918

(Garoia et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^MD-638, rho^UAS.cdCa has wing vein | ectopic phenotype, enhanceable by tay^RNAi.UAS/Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, rho^UAS.cdCa has embryonic/larval oenocyte precursor | increased number phenotype, suppressible by aos^UAS.cHa

(Brodu et al., 2004)

Scer\GAL4^en-e16E, rho^UAS.cdCa has oenocyte primordium phenotype, suppressible by aos^UAS.cHa

(Brodu et al., 2004)

Scer\GAL4^en-e16E, rho^UAS.cdCa has embryonic/larval oenocyte | increased number phenotype, suppressible by aos^UAS.cHa

(Brodu et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, rho^UAS.cdCa has oenocyte primordium phenotype, non-suppressible by ato¹/Df(3R)p13

(Brodu et al., 2004)

Scer\GAL4^en-e16E, rho^UAS.cdCa has embryonic/larval oenocyte precursor | increased number phenotype, non-suppressible by ato¹/Df(3R)p13

(Brodu et al., 2004)

Scer\GAL4^en-e16E, rho^UAS.cdCa has embryonic/larval oenocyte | increased number phenotype, non-suppressible by ato¹/Df(3R)p13

(Brodu et al., 2004)

Scer\GAL4^en-e16E, rho^UAS.cdCa has embryonic/larval oenocyte precursor | increased number phenotype, non-suppressible by N^ICN.UAS

(Brodu et al., 2004)

Scer\GAL4^en-e16E, rho^UAS.cdCa has embryonic/larval oenocyte | increased number phenotype, non-suppressible by N^ICN.UAS

(Brodu et al., 2004)

Scer\GAL4^en-e16E, rho^UAS.cdCa has oenocyte primordium phenotype, non-suppressible by N^ICN.UAS

(Brodu et al., 2004)

Enhancer of

Statement

Reference

rho^UAS.cdCa/Scer\GAL4^MD-638 is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^MD-638, tay^RNAi.UAS

(Molnar and de Celis, 2013)

rho^UAS.cdCa/Scer\GAL4^dpp.blk1 is an enhancer of eye disc phenotype of ft^G-rv

(Garoia et al., 2005)

rho^UAS.cdCa/Scer\GAL4^dpp.blk1 is an enhancer of wing disc phenotype of ft^G-rv

(Garoia et al., 2005)

rho^UAS.cdCa/Scer\GAL4^ey.PH is an enhancer of eye | somatic clone phenotype of ft^G-rv

(Garoia et al., 2005)

Suppressor of

Statement

Reference

rho^UAS.cdCa/Scer\GAL4^hh.PU is a suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

rho^UAS.cdCa/Scer\GAL4^Act.PU is a suppressor | somatic clone of eye disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ

(Sheng et al., 2016)

rho^UAS.cdCa/Scer\GAL4^en-e16E is a suppressor of embryonic/larval oenocyte precursor phenotype of ato¹/Df(3R)p13

(Brodu et al., 2004)

rho^UAS.cdCa/Scer\GAL4^en-e16E is a suppressor of oenocyte primordium phenotype of ato¹/Df(3R)p13

(Brodu et al., 2004)

rho^UAS.cdCa is a suppressor of oenocyte primordium phenotype of N^ICN.UAS, Scer\GAL4^en-e16E

(Brodu et al., 2004)

rho^UAS.cdCa is a suppressor of embryonic/larval oenocyte precursor phenotype of N^ICN.UAS, Scer\GAL4^en-e16E

(Brodu et al., 2004)

rho^UAS.cdCa/Scer\GAL4^en-e16E is a suppressor of embryonic/larval oenocyte phenotype of ato¹/Df(3R)p13

(Brodu et al., 2002)

NOT Suppressor of

Statement

Reference

rho^UAS.cdCa/Scer\GAL4^Act.PU is a non-suppressor | somatic clone of eye disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

rho^UAS.cdCa/Scer\GAL4^dpp.3KK is a non-suppressor of wing vein phenotype of Mkp3^EP-M76

(Ruiz-Gomez et al., 2005)

rho^UAS.cdCa is a non-suppressor of oenocyte | increased number phenotype of Scer\GAL4^en-e16E, aos^UAS.cHa

(Brodu et al., 2004)

rho^UAS.cdCa is a non-suppressor of C1 chordotonal organ precursor cell phenotype of N^ICN.UAS, Scer\GAL4^en-e16E

(Brodu et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ;Stam¹⁹ double homozygous somatic MARCM clones in third instar larval eye disc is not suppressed by expression of rho^{Scer\UAS.cdCa} under the control of the Scer\GAL4^Act.PU driver in the mutant clones.

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ homozygous somatic MARCM clones near the morphogenetic furrow in third instar larval eye disc is strongly suppressed by expression of rho^{Scer\UAS.cdCa} under the control of the Scer\GAL4^Act.PU driver in the mutant clones.

(Sheng et al., 2016)

Co-expression of rho^{Scer\UAS.cdCa} and tay^{dsRNA.Scer\UAS} under the control of Scer\GAL4^MD-638 results in a stronger ectopic wing vein phenotype than when either transgene is expressed alone.

(Molnar and de Celis, 2013)

When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^dpp.blk1 in a ft^G-rv background a significant enhancement is seen in the ft^G-rv overgrowth phenotype in the Scer\GAL4^dpp.blk1 expression territory. When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^ey.PH in ft^G-rv mutant eyes, significantly enlarged eyes are seen.

(Garoia et al., 2005)

Expression of either rho^{Scer\UAS.cdCa} does not affect the wing vein phenotype of Scer\GAL4^dpp.3KK>Mkp3^GSM76 flies.

(Ruiz-Gomez et al., 2005)

The oenocyte phenotype seen in rho^{Scer\UAS.cdCa}; Scer\GAL4^en-e16E embryos (extra waves of induction and delamination resulting in increased numbers of oenocytes in each cluster) is unaffected in a ato¹/Df(3R)p13 background or a N^ICN.Scer\UAS; Scer\GAL4^en-e16E (both of which lack chordotonal organ precursor C1).

argos^Scer\UAS.cHa; Scer\GAL4^en-e16E suppresses the increased numbers of oenocytes (and by implication, the number of waves of larval oenocyte precursor delamination) seen in rho^{Scer\UAS.cdCa}; Scer\GAL4^en-e16E embryos. A plot of the number of oenocytes per cluster peaks at 4, lower than the typical 6 oeoncytes per cluster seen in wild-type.

(Brodu et al., 2004)

The lack of oenocytes seen in ato¹/Df(3R)p13 embryos is rescued by expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^en-e16E.

(Brodu et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8858

w^*; P{UAS-ve.dC}2; D^r72/TM6B, P{iab-2(1.7)lacZ}6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

rho^{Scer\UAS.cdCa}

rho^UAS.cdCa

ve^{Scer\UAS.cdCa}

ve^UAS.cdCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of de Celis

(de Celis et al., 1997)

Secondary FlyBase IDs

References (21)

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