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General Information
Symbol
Dmel\rhoUAS.cdCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of de Celis
FlyBase ID
FBal0060846
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-rho, UAS-rhomboid-1, UAS-ve32, UAS-rho-1, UAS-rhomboid, UAS-rhomboid1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the rho coding sequence.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing rhoUAS.cdCa under the control of Scer\GAL4twi.2PE results in the near absence of embryonic ostial cardioblasts, despite of no significant effect in the overall number of cardioblasts.

Expression under the control of Scer\GAL4how-24B leads to a small but significant decrease in the number of embryonic ostial cardioblasts and a converse small but significant increase in the number of generic embryonic cardioblasts, leading to no change in the overall number of embryonic cardioblasts, as compared to controls.

Expression under the control of Scer\GAL4pnr-MD237 does not significantly affect the numbers of embryonic cardioblasts, as compared to controls.

Expression under the double control Scer\GAL4tin.cBa and Scer\GAL4tin.CΔ4 leads to a severe increase in the overall number of embryonic cardioblasts, namely of generic cardioblasts but not of embryonic ostial cardioblasts. This expression also leads to a significant increase in the number of odd and Tin-positive pericardial cells, although the number of even pericardial cells seems unaffected, as compared to controls.

Ectopic expression of rhoScer\UAS.cdCa under the control of Scer\GAL4GMR.PU results in supernumerary cells of all types in the pupal retina.

Expression via Scer\GAL4fkh.PH results in salivary glands positioned too anteriorly with no obvious distinction in shape between duct and secretory cells and no common duct-like structure formed at stage 15. At earlier stages during the invagination process, aberrantly shaped lumena are observed, but most prominently a large bulge of eyg-positive ectopic cells seem to arise between the already invaginated secretory gland portions at the position where the individual ducts would normally form.

Expression via Scer\GAL4arm.PU leads to embryos with varying degrees of affected morphology (in many cases head involution fails) and the general appearance of the epidermis is less organized compared to wild type, although epithelial integrity/polarity appears unperturbed. Only very short glands invaginate into the embryo, and a large bulge of eyg-positive cells is found at the surface of the embryo between the two invagination sides.

Whereas control salivary gland placode cells at stages 12-13 do not undergo division, Scer\GAL4fkh.PH rhoScer\UAS.cdCa placode cells are actively dividing.

Overexpression of rhoScer\UAS.cdCa, under the control of Scer\GAL4salm-459.2, gives rise to an invagination phenotype. Two initial invagination sites appear in the placode, and cells from the dorsal side do not rotate, leading to the formation of a double arch at the early stage 11 placode.

When rhoScer\UAS.cdCa is driven by Scer\GAL4dpp.blk1 no effect is seen on wing disc growth.

In rhoScer\UAS.cdCa; Scer\GAL4en-e16E embryos, induction of larval oenocyte precursors begins at the normal time (during stage 9) and is followed by two waves of delamination with the number of cells (average 3 per wave from each oenocyte primordium) and timing of delamination as in wild-type. However, in these embryos, additional (heterochronic) waves of induction and delamination occur after these two waves (throughout stage 12), resulting in increased numbers of mature oenocytes in stage 16 embryos. Oenocyte clusters in these embryos show a multi-modal distribution of cell numbers with peaks corresponding to multiples of 3 (12, 15, 18 and 21 compared to the wild-type average of 6 resulting from 2 delamination cycles), suggesting there are up to 4 additional waves of delamination. The additional pulses of larval oenocyte precursor delamination occur with the same periodicity as the first two phases in wild-type.

When rhoScer\UAS.cdCa is driven by Scer\GAL4sim.PS an increase is see in the number of proliferating midbrain cells.

Expression of rhoScer\UAS.cdCa under the control of Scer\GAL4en-e16E or Scer\GAL4ato.3.6 results in the formation of ectopic oenocytes in the T1-T3 segments (they are normally only found in segments A1-A7).

rhoScer\UAS.cdCa; Scer\GAL4Bx-MS1096 flies have blistered wings with large amounts of ectopic vein material.

The number of chordotonal organs in the lateral cluster is increased from 5 to 6 in embryos expressing rhoScer\UAS.cdCa under the control of Scer\GAL4en-e16E. In stage 11 embryos, the oenocyte precursor whorl is enlarged and by stage 16 oenocyte clusters containing 17-27 cells are seen.

Expression of rhoScer\UAS.cdCa under the control of Scer\GAL4Bx-MS1096 results in small, heavily pigmented wings with excess vein material.

Expression of rhoScer\UAS.cdCa under the control of Scer\GAL4GMR.PF disrupts eye development leading to a rough eye phenotype. At the cellular level excess cone and pigment cell recruitment is seen, excess photoreceptors are also sometimes seen. When rhoScer\UAS.cdCa is expressed under the control of Scer\GAL4Bx-MS1096, leads to small, pigmented, blistered wings: all cells in the wing are concerted to vein cells. When rhoScer\UAS.cdCa is expressed under the control of Scer\GAL4CY2, embryos have a dorsalised phenotype.

Scer\GAL4c179-mediated expression of HLHmβScer\UAS.cdCa in the wing causes deletion of most veins and Scer\GAL4salm-459.2-mediated expression causes truncation of vein LIV. Expression of rhoScer\UAS.cdCa rescues vein loss and results in thicker veins.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ;Stam19 double homozygous somatic MARCM clones in third instar larval eye disc is not suppressed by expression of rhoScer\UAS.cdCa under the control of the Scer\GAL4Act.PU driver in the mutant clones.

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ homozygous somatic MARCM clones near the morphogenetic furrow in third instar larval eye disc is strongly suppressed by expression of rhoScer\UAS.cdCa under the control of the Scer\GAL4Act.PU driver in the mutant clones.

Co-expression of rhoScer\UAS.cdCa and taydsRNA.Scer\UAS under the control of Scer\GAL4MD-638 results in a stronger ectopic wing vein phenotype than when either transgene is expressed alone.

When rhoScer\UAS.cdCa is driven by Scer\GAL4dpp.blk1 in a ftG-rv background a significant enhancement is seen in the ftG-rv overgrowth phenotype in the Scer\GAL4dpp.blk1 expression territory. When rhoScer\UAS.cdCa is driven by Scer\GAL4ey.PH in ftG-rv mutant eyes, significantly enlarged eyes are seen.

Expression of either rhoScer\UAS.cdCa does not affect the wing vein phenotype of Scer\GAL4dpp.3KK>Mkp3GSM76 flies.

The oenocyte phenotype seen in rhoScer\UAS.cdCa; Scer\GAL4en-e16E embryos (extra waves of induction and delamination resulting in increased numbers of oenocytes in each cluster) is unaffected in a ato1/Df(3R)p13 background or a NICN.Scer\UAS; Scer\GAL4en-e16E (both of which lack chordotonal organ precursor C1).

argosScer\UAS.cHa; Scer\GAL4en-e16E suppresses the increased numbers of oenocytes (and by implication, the number of waves of larval oenocyte precursor delamination) seen in rhoScer\UAS.cdCa; Scer\GAL4en-e16E embryos. A plot of the number of oenocytes per cluster peaks at 4, lower than the typical 6 oeoncytes per cluster seen in wild-type.

The lack of oenocytes seen in ato1/Df(3R)p13 embryos is rescued by expression of rhoScer\UAS.cdCa under the control of Scer\GAL4en-e16E.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
rhoScer\UAS.cdCa
rhoUAS.cdCa
veScer\UAS.cdCa
veUAS.cdCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of de Celis
Secondary FlyBase IDs
    References (20)