Allele Dmel\rho^{Scer\UAS.cdCa}

General Information
Symbol	Dmel\rho^{Scer\UAS.cdCa}	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of de Celis	FlyBase ID	FBal0060846
Feature type	allele	Associated gene	Dmel\rho

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (de Celis et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: rho coding sequence cloned into pUAST. (de Celis et al., 1997)
Carried in construct	P{UAS-ve.dC} (Ruiz-Gomez et al., 2005, Brodu et al., 2002, Lee et al., 2001, Urban et al., 2002, Elstob et al., 2001, Wasserman et al., 2000, Brodu et al., 2004, Garoia et al., 2005, Page, 2003, de Celis et al., 1997, Brodu and Casanova, 2006, Molnar et al., 2006, Wojcinski et al., 2011, Moses et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^Bx-MS1096 (Wasserman et al., 2000, Lee et al., 2001) visible, with Scer\GAL4^CY2 (Wasserman et al., 2000) visible, with Scer\GAL4^GMR.PF (Wasserman et al., 2000)
Phenotype Manifest In
	abdominal lateral pentascolopidial chordotonal organ lch5 \| supernumerary, with Scer\GAL4^en-e16E (Elstob et al., 2001) cone cell \| ectopic, with Scer\GAL4^GMR.PF (Wasserman et al., 2000) egg, with Scer\GAL4^CY2 (Wasserman et al., 2000) embryonic/larval oenocyte \| ectopic, with Scer\GAL4^ato.3.6 (Brodu et al., 2002) embryonic/larval oenocyte \| ectopic, with Scer\GAL4^en-e16E (Brodu et al., 2002) embryonic/larval oenocyte \| supernumerary, with Scer\GAL4^en-e16E (Elstob et al., 2001, Brodu et al., 2004) embryonic/larval oenocyte precursor \| supernumerary, with Scer\GAL4^en-e16E (Brodu et al., 2004) embryonic central brain, with Scer\GAL4^sim.PS (Page, 2003) embryonic mesothoracic segment, with Scer\GAL4^ato.3.6 (Brodu et al., 2002) embryonic mesothoracic segment, with Scer\GAL4^en-e16E (Brodu et al., 2002) embryonic metathoracic segment, with Scer\GAL4^ato.3.6 (Brodu et al., 2002) embryonic metathoracic segment, with Scer\GAL4^en-e16E (Brodu et al., 2002) embryonic prothoracic segment, with Scer\GAL4^ato.3.6 (Brodu et al., 2002) embryonic prothoracic segment, with Scer\GAL4^en-e16E (Brodu et al., 2002) eye, with Scer\GAL4^GMR.PF (Wasserman et al., 2000) eye photoreceptor cell \| ectopic, with Scer\GAL4^GMR.PF (Wasserman et al., 2000) oenocyte primordium, with Scer\GAL4^en-e16E (Brodu et al., 2004) pigment cell \| ectopic, with Scer\GAL4^GMR.PF (Wasserman et al., 2000) presumptive embryonic/larval tracheal system, with Scer\GAL4^salm-459.2 (Brodu and Casanova, 2006) tracheal primordium, with Scer\GAL4^salm-459.2 (Brodu and Casanova, 2006) wing, with Scer\GAL4^Bx-MS1096 (Lee et al., 2001, Urban et al., 2002) wing vein \| ectopic, with Scer\GAL4^Bx-MS1096 (Wasserman et al., 2000, Urban et al., 2002) wing vein \| supernumerary, with Scer\GAL4^Bx-MS1096 (Lee et al., 2001)
Detailed Description
	Statement Reference Overexpression of rho^{Scer\UAS.cdCa}, under the control of Scer\GAL4^salm-459.2, gives rise to an invagination phenotype. Two initial invagination sites appear in the placode, and cells from the dorsal side do not rotate, leading to the formation of a double arch at the early stage 11 placode. (Brodu and Casanova, 2006) When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^dpp.blk1 no effect is seen on wing disc growth. (Garoia et al., 2005) In rho[Scer\UAS.cdCa]; Scer\GAL4[en-e16E] embryos, induction of larval oenocyte precursors begins at the normal time (during stage 9) and is followed by two waves of delamination with the number of cells (average 3 per wave from each oenocyte primordium) and timing of delamination as in wild-type. However, in these embryos, additional (heterochronic) waves of induction and delamination occur after these two waves (throughout stage 12), resulting in increased numbers of mature oenocytes in stage 16 embryos. Oenocyte clusters in these embryos show a multi-modal distribution of cell numbers with peaks corresponding to multiples of 3 (12, 15, 18 and 21 compared to the wild-type average of 6 resulting from 2 delamination cycles), suggesting there are up to 4 additional waves of delamination. The additional pulses of larval oenocyte precursor delamination occur with the same periodicity as the first two phases in wild-type. (Brodu et al., 2004) When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^sim.PS an increase is see in the number of proliferating midbrain cells. (Page, 2003) Expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^en-e16E or Scer\GAL4^ato.3.6 results in the formation of ectopic oenocytes in the T1-T3 segments (they are normally only found in segments A1-A7). (Brodu et al., 2002) rho^{Scer\UAS.cdCa}; Scer\GAL4^Bx-MS1096 flies have blistered wings with large amounts of ectopic vein material. (Urban et al., 2002) The number of chordotonal organs in the lateral cluster is increased from 5 to 6 in embryos expressing rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^en-e16E. In stage 11 embryos, the oenocyte precursor whorl is enlarged and by stage 16 oenocyte clusters containing 17-27 cells are seen. (Elstob et al., 2001) Expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^Bx-MS1096 results in small, heavily pigmented wings with excess vein material. (Lee et al., 2001) Expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^GMR.PF disrupts eye development leading to a rough eye phenotype. At the cellular level excess cone and pigment cell recruitment is seen, excess photoreceptors are also sometimes seen. When rho^{Scer\UAS.cdCa} is expressed under the control of Scer\GAL4^Bx-MS1096, leads to small, pigmented, blistered wings: all cells in the wing are concerted to vein cells. When rho^{Scer\UAS.cdCa} is expressed under the control of Scer\GAL4^CY2, embryos have a dorsalised phenotype. (Wasserman et al., 2000) Scer\GAL4^c179-mediated expression of HLHmβ^{Scer\UAS.cdCa} in the wing causes deletion of most veins and Scer\GAL4^salm-459.2-mediated expression causes truncation of vein LIV. Expression of rho^{Scer\UAS.cdCa} rescues vein loss and results in thicker veins. (de Celis et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference rho^{Scer\UAS.cdCa}/Scer\GAL4^Bx-MS1096 is an enhancer of increased cell number \| somatic clone phenotype of ft^k07918 (Garoia et al., 2005) rho^{Scer\UAS.cdCa}/Scer\GAL4^dpp.blk1 is an enhancer of increased cell number phenotype of ft^G-rv (Garoia et al., 2005)
NOT Enhancer of
Statement Reference rho^{Scer\UAS.cdCa}/Scer\GAL4^Bx-MS1096 is a non-enhancer of planar polarity defective \| somatic clone phenotype of ft^k07918 (Garoia et al., 2005)
NOT Suppressor of
Statement Reference rho^{Scer\UAS.cdCa}/Scer\GAL4^Bx-MS1096 is a non-suppressor of planar polarity defective \| somatic clone phenotype of ft^k07918 (Garoia et al., 2005)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has embryonic/larval oenocyte \| supernumerary phenotype, suppressible by aos^Scer\UAS.cHa (Brodu et al., 2004) Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has embryonic/larval oenocyte precursor \| supernumerary phenotype, suppressible by aos^Scer\UAS.cHa (Brodu et al., 2004) Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has oenocyte primordium phenotype, suppressible by aos^Scer\UAS.cHa (Brodu et al., 2004)
NOT suppressed by
Statement Reference Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has embryonic/larval oenocyte \| supernumerary phenotype, non-suppressible by ato¹/Df(3R)p13 (Brodu et al., 2004) Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has embryonic/larval oenocyte \| supernumerary phenotype, non-suppressible by N^ICN.Scer\UAS (Brodu et al., 2004) Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has embryonic/larval oenocyte precursor \| supernumerary phenotype, non-suppressible by ato¹/Df(3R)p13 (Brodu et al., 2004) Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has embryonic/larval oenocyte precursor \| supernumerary phenotype, non-suppressible by N^ICN.Scer\UAS (Brodu et al., 2004) Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has oenocyte primordium phenotype, non-suppressible by ato¹/Df(3R)p13 (Brodu et al., 2004) Scer\GAL4^en-e16E, rho^{Scer\UAS.cdCa} has oenocyte primordium phenotype, non-suppressible by N^ICN.Scer\UAS (Brodu et al., 2004)
Enhancer of
Statement Reference rho^{Scer\UAS.cdCa}/Scer\GAL4^dpp.blk1 is an enhancer of eye disc phenotype of ft^G-rv (Garoia et al., 2005) rho^{Scer\UAS.cdCa}/Scer\GAL4^dpp.blk1 is an enhancer of wing disc phenotype of ft^G-rv (Garoia et al., 2005) rho^{Scer\UAS.cdCa}/Scer\GAL4^ey.PH is an enhancer of eye \| somatic clone phenotype of ft^G-rv (Garoia et al., 2005)
Suppressor of
Statement Reference rho^{Scer\UAS.cdCa}/Scer\GAL4^en-e16E is a suppressor of embryonic/larval oenocyte phenotype of ato¹/Df(3R)p13 (Brodu et al., 2002) rho^{Scer\UAS.cdCa}/Scer\GAL4^en-e16E is a suppressor of embryonic/larval oenocyte precursor phenotype of ato¹/Df(3R)p13 (Brodu et al., 2004) rho^{Scer\UAS.cdCa}/Scer\GAL4^en-e16E is a suppressor of oenocyte primordium phenotype of ato¹/Df(3R)p13 (Brodu et al., 2004) rho^{Scer\UAS.cdCa} is a suppressor of embryonic/larval oenocyte precursor phenotype of N^ICN.Scer\UAS, Scer\GAL4^en-e16E (Brodu et al., 2004) rho^{Scer\UAS.cdCa} is a suppressor of oenocyte primordium phenotype of N^ICN.Scer\UAS, Scer\GAL4^en-e16E (Brodu et al., 2004)
NOT Suppressor of
Statement Reference rho^{Scer\UAS.cdCa}/Scer\GAL4^dpp.3KK is a non-suppressor of wing vein phenotype of Mkp3^GSM76 (Ruiz-Gomez et al., 2005) rho^{Scer\UAS.cdCa} is a non-suppressor of C1 chordotonal organ precursor cell phenotype of N^ICN.Scer\UAS, Scer\GAL4^en-e16E (Brodu et al., 2004) rho^{Scer\UAS.cdCa} is a non-suppressor of oenocyte \| supernumerary phenotype of Scer\GAL4^en-e16E, aos^Scer\UAS.cHa (Brodu et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^dpp.blk1 in a ft^G-rv background a significant enhancement is seen in the ft^G-rv overgrowth phenotype in the Scer\GAL4^dpp.blk1 expression territory. When rho^{Scer\UAS.cdCa} is driven by Scer\GAL4^ey.PH in ft^G-rv mutant eyes, significantly enlarged eyes are seen. (Garoia et al., 2005) Expression of either rho^{Scer\UAS.cdCa} does not affect the wing vein phenotype of Scer\GAL4^dpp.3KK>Mkp3^GSM76 flies. (Ruiz-Gomez et al., 2005) The oenocyte phenotype seen in rho[Scer\UAS.cdCa]; Scer\GAL4[en-e16E] embryos (extra waves of induction and delamination resulting in increased numbers of oenocytes in each cluster) is unaffected in a ato[1]/Df(3R)p13 background or a N[ICN.Scer\UAS]; Scer\GAL4[en-e16E] (both of which lack chordotonal organ precursor C1). argos[Scer\UAS.cHa]; Scer\GAL4[en-e16E] suppresses the increased numbers of oenocytes (and by implication, the number of waves of larval oenocyte precursor delamination) seen in rho[Scer\UAS.cdCa]; Scer\GAL4[en-e16E] embryos. A plot of the number of oenocytes per cluster peaks at 4, lower than the typical 6 oeoncytes per cluster seen in wild-type. (Brodu et al., 2004) The lack of oenocytes seen in ato¹/Df(3R)p13 embryos is rescued by expression of rho^{Scer\UAS.cdCa} under the control of Scer\GAL4^en-e16E. (Brodu et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	8858 w^*; P{UAS-ve.dC}2; D^r72/TM6B, P{iab-2(1.7)lacZ}6B, Tb⁺
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	rho^{Scer\UAS.cdCa} ve^{Scer\UAS.cdCa} ve^UAS.cdCa
Name Synonym	Saccharomyces cerevisiae UAS construct a of de Celis (de Celis et al., 1997)
Secondary FlyBase IDs

References ( 14 )
Research paper	Moses et al., 2011, Dev. Biol. 360(1): 208--215 Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression. [FBrf0216621] Wojcinski et al., 2011, Dev. Biol. 358(1): 168--180 DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop. [FBrf0215253] Brodu and Casanova, 2006, Genes Dev. 20(13): 1817--1828 The RhoGAP crossveinless-c links trachealess and EGFR signaling to cell shape remodeling in Drosophila tracheal invagination. [FBrf0195282] Molnar et al., 2006, Genetics 174(3): 1635--1659 A gain-of-function screen identifying genes required for vein formation in the Drosophila melanogaster wing. [FBrf0195387] Garoia et al., 2005, Mech. Dev. 122(2): 175--187 The tumor suppressor gene fat modulates the EGFR-mediated proliferation control in the imaginal tissues of Drosophila melanogaster. [FBrf0184134] Ruiz-Gomez et al., 2005, Dev. Dyn. 232(3): 695--708 Conserved cross-interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual-specificity phosphatase MKP3. [FBrf0183898] Brodu et al., 2004, Dev. Cell 7(6): 885--895 EGF receptor signaling regulates pulses of cell delamination from the Drosophila ectoderm. [FBrf0180129] Page, 2003, Curr. Biol. 13(6): 474--482 A function for EGF receptor signaling in expanding the developing brain in Drosophila. [FBrf0158813] Brodu et al., 2002, Development 129(12): 2957--2963 abdominal A specifies one cell type in Drosophila by regulating one principal target gene. [FBrf0148967] Urban et al., 2002, EMBO J. 21(16): 4277--4286 A family of Rhomboid intramembrane proteases activates all Drosophila membrane-tethered EGF ligands. [FBrf0151285] Elstob et al., 2001, Development 128(5): 723--732 spalt-dependent switching between two cell fates that are induced by the Drosophila EGF receptor. [FBrf0134578] Lee et al., 2001, Cell 107(2): 161--171 Regulated intracellular ligand transport and proteolysis control EGF signal activation in Drosophila. [FBrf0139606] Wasserman et al., 2000, Genes Dev. 14(13): 1651--1663 A family of rhomboid-like genes: Drosophila rhomboid-1 and roughoid/rhomboid-3 cooperate to activate EGF receptor signaling. [FBrf0128677] de Celis et al., 1997, Development 124(10): 1919--1928 Notch signalling regulates veinlet expression and establishes boundaries between veins and interveins in the Drosophila wing. [FBrf0093808]

Allele Dmel\rhoScer\UAS.cdCa

Allele Dmel\rho^{Scer\UAS.cdCa}