Allele Dmel\ttk^p69.Scer\UAS

General Information
Symbol	Dmel\ttk^p69.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0060851
Feature type	allele	Associated gene	Dmel\ttk

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Giesen et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: cDNA clone expressing the p69 protein of ttk is cloned into vector pUAST. (Giesen et al., 1997)
Carried in construct	P{UAS-ttk.p69} (Pi et al., 2001, Yuasa et al., 2003, Hummel et al., 2002, French et al., 2003, Ward et al., 2002, Merabet et al., 2002, Nicolai et al., 2003, Kumar and Moses, 2001, Li et al., 1997, Giesen et al., 1997, Kumar and Moses, 2001, Li et al., 2009, Boyle and Berg, 2009, Siddall et al., 2009, Araujo et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size \| somatic clone, with Scer\GAL4^e22c (Boyle and Berg, 2009) lethal, with Scer\GAL4^cb01 (Ward et al., 2002) lethal, with Scer\GAL4^cb04 (Ward et al., 2002) lethal, with Scer\GAL4^cb06 (Ward et al., 2002) lethal, with Scer\GAL4^cb07 (Ward et al., 2002) lethal, with Scer\GAL4^cb08 (Ward et al., 2002) lethal, with Scer\GAL4^cb09 (Ward et al., 2002) lethal, with Scer\GAL4^cb10 (Ward et al., 2002) lethal, with Scer\GAL4^cb13 (Ward et al., 2002) lethal, with Scer\GAL4^cb15 (Ward et al., 2002) lethal, with Scer\GAL4^cb20 (Ward et al., 2002) lethal, with Scer\GAL4^cb21 (Ward et al., 2002) lethal, with Scer\GAL4^cb22 (Ward et al., 2002) lethal, with Scer\GAL4^cb24 (Ward et al., 2002) lethal, with Scer\GAL4^cb26 (Ward et al., 2002) lethal, with Scer\GAL4^cb28 (Ward et al., 2002) lethal, with Scer\GAL4^cb29 (Ward et al., 2002) lethal, with Scer\GAL4^cb30 (Ward et al., 2002) lethal, with Scer\GAL4^cb32 (Ward et al., 2002) lethal, with Scer\GAL4^cb39 (Ward et al., 2002) neuroanatomy defective, with Scer\GAL4^c739 (Nicolai et al., 2003) viable, with Scer\GAL4^cb03 (Ward et al., 2002) viable, with Scer\GAL4^cb05 (Ward et al., 2002) viable, with Scer\GAL4^cb11 (Ward et al., 2002) viable, with Scer\GAL4^cb12 (Ward et al., 2002) viable, with Scer\GAL4^cb17 (Ward et al., 2002) viable, with Scer\GAL4^cb19 (Ward et al., 2002) viable, with Scer\GAL4^cb23 (Ward et al., 2002) viable, with Scer\GAL4^cb25 (Ward et al., 2002) viable, with Scer\GAL4^cb27 (Ward et al., 2002) viable, with Scer\GAL4^cb31 (Ward et al., 2002) viable, with Scer\GAL4^cb34 (Ward et al., 2002) viable, with Scer\GAL4^cb35 (Ward et al., 2002) viable, with Scer\GAL4^cb36 (Ward et al., 2002) viable, with Scer\GAL4^cb37 (Ward et al., 2002) viable, with Scer\GAL4^cb38 (Ward et al., 2002) viable, with Scer\GAL4^cb40 (Ward et al., 2002) visible, with Scer\GAL4^Eq1 (Pi et al., 2001) visible, with Scer\GAL4^ey.PB (Kumar and Moses, 2001)
Phenotype Manifest In
	cone cell, with Scer\GAL4^GMR.PF (Li et al., 1997) cone cell \| third instar larval stage, with Scer\GAL4^GMR.PF (Siddall et al., 2009) cone cell \| third instar larval stage, with Scer\GAL4^lz-gal4 (Siddall et al., 2009) embryonic/larval dorsal trunk, with Scer\GAL4^btl.PS (Araujo et al., 2007) eye, with Scer\GAL4^ey.PB (Kumar and Moses, 2001) eye, with Scer\GAL4^GMR.PF (Li et al., 1997, Siddall et al., 2009) eye, with Scer\GAL4^lz-gal4 (Siddall et al., 2009) eye photoreceptor cell, with Scer\GAL4^GMR.PF (Li et al., 1997) interommatidial bristle, with Scer\GAL4^GMR.PF (Li et al., 1997) Kenyon cell, with Scer\GAL4^c739 (Nicolai et al., 2003) lamina anlage glial cell, with Scer\GAL4^c527 (Hummel et al., 2002) lamina neuropil, with Scer\GAL4^c527 (Hummel et al., 2002) microchaeta, with Scer\GAL4^Eq1 (Pi et al., 2001) morphogenetic furrow, with Scer\GAL4^dpp.blk1 (Kumar and Moses, 2001) mushroom body, with Scer\GAL4^c739 (Nicolai et al., 2003) ommatidium, with Scer\GAL4^lz-gal4 (Siddall et al., 2009) ommatidium \| third instar larval stage, with Scer\GAL4^GMR.PF (Siddall et al., 2009) oocyte \| oogenesis stage S10B \| somatic clone, with Scer\GAL4^e22c (Boyle and Berg, 2009) peripheral nervous system, with Scer\GAL4^Kr.PM (Giesen et al., 1997) photoreceptor cell R1 \| third instar larval stage, with Scer\GAL4^GMR.PF (Siddall et al., 2009) photoreceptor cell R1 \| third instar larval stage, with Scer\GAL4^lz-gal4 (Siddall et al., 2009) photoreceptor cell R6 \| third instar larval stage, with Scer\GAL4^GMR.PF (Siddall et al., 2009) photoreceptor cell R6 \| third instar larval stage, with Scer\GAL4^lz-gal4 (Siddall et al., 2009) photoreceptor cell R7 \| third instar larval stage, with Scer\GAL4^GMR.PF (Siddall et al., 2009) photoreceptor cell R7 \| third instar larval stage, with Scer\GAL4^lz-gal4 (Siddall et al., 2009) subretinal glial cell, with Scer\GAL4^c527 (Hummel et al., 2002)
Detailed Description
	Statement Reference Follicular cells expressing ttk[p69.Scer\UAS] under the control of Scer\GAL4[e22c] are reduced in size. (Boyle and Berg, 2009) Expression of ttk[p69.Scer\UAS] under the control of Scer\GAL4[lz-gal4] results in severely disrupted adult eyes that show scarring and lack ommatidial structures. In third instar larval eye discs ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells. Expression of ttk[p69.Scer\UAS] under the control of Scer\GAL4[GMR.PF] results in severely perturbed adult eyes, displaying no ommatidial structure. Ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells. (Siddall et al., 2009) Expression of ttk[p69.Scer\UAS] under the control of Scer\GAL4[btl.PS] results in impaired tracheal branch fusion. (Araujo et al., 2007) When expression is driven by Scer\GAL4^c739, ttk^p69.Scer\UAS shows a mushroom body Class I phenotype; neuron number reduced. This phenotype is similar to, but less extreme than, that caused by ttk^UY181 driven by Scer\GAL4^c739. Loss of neurons is not accompanied by increase in number of glial cells. (Nicolai et al., 2003) When Scer\GAL4^c527 is expressed under the control of Scer\GAL4^c527 in third instar larvae, defects are seen in glial cell migration in the developing eye. In about 20% of these animals surface glial cells do not enter the eye disc and R cell axons fail to enter the optic stalk forming a mass of R cell axons in the basal region of the disc. In the remaining larvae, fewer surface glial cells are found in the eye disc. In these discs R cell axons project into the optic stalk, but are highly disorganised. (Hummel et al., 2002) Flies expressing ttk^p69.Scer\UAS under the control of Scer\GAL4^cb12, Scer\GAL4^cb19, Scer\GAL4^cb35, Scer\GAL4^cb36, Scer\GAL4^cb37 or Scer\GAL4^cb38 are moderately viable. Flies expressing ttk^p69.Scer\UAS under the control of Scer\GAL4^cb05 or Scer\GAL4^cb23 are viable at 18^oC. (Ward et al., 2002) Expression of ttk^p69.Scer\UAS under the control of Scer\GAL4^ey.PB results in deletion of the eye. (Kumar and Moses, 2001) Expression of ttk^p69.Scer\UAS under the control of Scer\GAL4^dpp.blk1 affects furrow reincarnation but not birth in the eye disc. (Kumar and Moses, 2001) Expression of ttk^p69.Scer\UAS under the control of Scer\GAL4^Eq1 results in a lack of most microchaetae. (Pi et al., 2001) Scer\GAL4^Kr.PM-mediated expression leads to a partial block of neuronal development in the PNS and CNS. Scer\GAL4^sca-537.4-mediated expression impairs neuronal differentiation. In late stage 16/17 embryos cell death is observed. (Giesen et al., 1997) Eyes are deformed, severely reduced in size and lack bristles, cone cells and photoreceptor cells in flies expressing ttk^p69.Scer\UAS under the control of Scer\GAL4^GMR.PF. (Li et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^p69.Scer\UAS has cone cell \| third instar larval stage phenotype, suppressible \| partially by lz^GMR.PD (Siddall et al., 2009) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^p69.Scer\UAS has ommatidium \| third instar larval stage phenotype, suppressible \| partially by lz^GMR.PD (Siddall et al., 2009) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^p69.Scer\UAS has photoreceptor cell R1 \| third instar larval stage phenotype, suppressible \| partially by lz^GMR.PD (Siddall et al., 2009) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^p69.Scer\UAS has photoreceptor cell R6 \| third instar larval stage phenotype, suppressible \| partially by lz^GMR.PD (Siddall et al., 2009) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, ttk^p69.Scer\UAS has photoreceptor cell R7 \| third instar larval stage phenotype, suppressible \| partially by lz^GMR.PD (Siddall et al., 2009)
Suppressor of
Statement Reference ttk^p69.Scer\UAS/Scer\GAL4^Eq1 is a suppressor of mesothoracic tergum & microchaeta \| ectopic phenotype of Scer\GAL4^Eq1, phyl^Scer\UAS.cPa (Pi et al., 2001)
Other
Statement Reference Scer\GAL4^sca-T3, repo^Scer\UAS.cYa, ttk^p69.Scer\UAS has embryonic/larval neuron phenotype (Yuasa et al., 2003, Yuasa et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference Over-expression of lz[GMR.PD] in flies expressing ttk[p69.Scer\UAS] in under the control of Scer\GAL4[GMR.PF] partially suppresses the loss cone cells and R1, R6 and R7 photoreceptor cells. (Siddall et al., 2009) Co-expression of repo^Scer\UAS.cYa and ttk^p69.Scer\UAS under the control of Scer\GAL4^sca-T3 results in a synergistic suppression of neuronal development in embryos. (Yuasa et al., 2003) Most notal microchaetae are missing in animals coexpressing phyl^Scer\UAS.cPa and ttk^p69.Scer\UAS under the control of Scer\GAL4^Eq1. (Pi et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	ttk^p69.Scer\UAS/Scer\GAL4^btl.PS rescues ttk^D2-50 (Araujo et al., 2007)
Partially rescues	ttk^p69.Scer\UAS/Scer\GAL4^hs.PB partially rescues ttk^twp/ttk^1e11 (French et al., 2003) ttk^p69.Scer\UAS/Scer\GAL4^sli.PS partially rescues ttk^B330 (Giesen et al., 1997)
Comments	Expression of ttk[p69.Scer\UAS] under the control of Scer\GAL4[btl.PS] in ttk[D2-50] mutant animals rescues the tracheal intercalation defects. Terminal branch fusion defects of the lateral trunk are also rescued in these animals. (Araujo et al., 2007) Short heat shocks and moderate temperatures in ttk^twp/ttk^twp, ttk^p69.Scer\UAS Scer\GAL4^hs.PB animals lead to significant partial rescue of dorsal appendage and chorion defects. Eggs are normal in shape and size and dorsal appendages are significantly longer than seen in the mutants alone. Longer heat shocks lead to a dramatic decline in rescue frequency accompanied by an increase in defects early in oogenesis. (French et al., 2003) Scer\GAL4^sli.PS-mediated expression partially rescues the CNS phenotype with two copies of the construct. (Giesen et al., 1997)
Stocks ( 1 )
Bloomington	7361 w^*; P{UAS-ttk.p69}2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ttk^p69.Scer\UAS ttk^p69.UAS
Name Synonym
Secondary FlyBase IDs

References ( 15 )
Research paper	Boyle and Berg, 2009, Development 136(24): 4187--4197 Control in time and space: Tramtrack69 cooperates with Notch and Ecdysone to repress ectopic fate and shape changes during Drosophila egg chamber maturation. [FBrf0209360] Li et al., 2009, Cell 137(2): 273--282 A microRNA imparts robustness against environmental fluctuation during development. [FBrf0207846] Siddall et al., 2009, BMC Dev. Biol. 9: 64 Ttk69-dependent repression of lozenge prevents the ectopic development of R7 cells in the Drosophila larval eye disc. [FBrf0209609] Araujo et al., 2007, Development 134(20): 3665--3676 Tramtrack regulates different morphogenetic events during Drosophila tracheal development. [FBrf0201143] French et al., 2003, Dev. Biol. 253(1): 18--35 The Drosophila female sterile mutation twin peaks is a novel allele of tramtrack and reveals a requirement for Ttk69 in epithelial morphogenesis. [FBrf0155483] Nicolai et al., 2003, J. Neurobiol. 57(3): 291--302 Gain-of-function screen identifies a role of the Src64 oncogene in Drosophila mushroom body development. [FBrf0167790] Yuasa et al., 2003, Development 130(11): 2419--2428 Drosophila homeodomain protein REPO controls glial differentiation by cooperating with ETS and BTB transcription factors. [FBrf0158889] Hummel et al., 2002, Neuron 33(2): 193--203 Temporal control of glial cell migration in the Drosophila eye requires gilgamesh, hedgehog, and eye specification genes. [FBrf0145174] Merabet et al., 2002, Genetics 162(1): 189--202 A green fluorescent protein reporter genetic screen that identifies modifiers of Hox gene function in the Drosophila embryo. [FBrf0152032] Ward et al., 2002, genesis 34(1-2): 46--50 GAL4 enhancer trap patterns during Drosophila development. [FBrf0152375] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] Pi et al., 2001, Development 128(14): 2699--2710 A dual function of phyllopod in Drosophila external sensory organ development: cell fate specification of sensory organ precursor and its progeny. [FBrf0138359] Giesen et al., 1997, Development 124(12): 2307--2316 Glial development in the Drosophila CNS requires concomitant activation of glial and repression of neuronal differentiation genes. [FBrf0094782] Li et al., 1997, Cell 90(3): 469--478 Photoreceptor cell differentiation requires regulated proteolysis of the transcriptional repressor tramtrack. [FBrf0098298]

Allele Dmel\ttkp69.Scer\UAS

Allele Dmel\ttk^p69.Scer\UAS