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General Information
Symbol
Dmel\ttkp69.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0060851
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ttk69
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a cDNA clone expressing the p69 isoform of ttk.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-specific expression of ttkp69.UAS under the control of Scer\GAL4GMR44E10 leads to females with a severely decreased egg-laying rate and significant retention of mature follicles in the ovary, as compared to controls. Consistently, follicles rupture much less frequently upon induction by OA or ionomycin, as compared to controls.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4twi.PB and Scer\GAL4how-24B leads to lethality at embryonic stages due to severe defects in the specification of all three muscle types. The heart is either completely absent or only a few isolated cardioblasts are visible, visceral musculature contains gaps and has abnormal organization, somatic musculature contains very few correctly specific and differentiated muscle fibers, with cells that remain mononucleated and form small muscles that lack clear identity.

Follicular cells expressing ttkp69.Scer\UAS under the control of Scer\GAL4e22c are reduced in size.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4lz-gal4 results in severely disrupted adult eyes that show scarring and lack ommatidial structures. In third instar larval eye discs ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4GMR.PF results in severely perturbed adult eyes, displaying no ommatidial structure. Ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4btl.PS results in impaired tracheal branch fusion.

When expression is driven by Scer\GAL4c739, ttkp69.Scer\UAS shows a mushroom body Class I phenotype; neuron number reduced. This phenotype is similar to, but less extreme than, that caused by ttkUY181 driven by Scer\GAL4c739. Loss of neurons is not accompanied by increase in number of glial cells.

When Scer\GAL4c527 is expressed under the control of Scer\GAL4c527 in third instar larvae, defects are seen in glial cell migration in the developing eye. In about 20% of these animals surface glial cells do not enter the eye disc and R cell axons fail to enter the optic stalk forming a mass of R cell axons in the basal region of the disc. In the remaining larvae, fewer surface glial cells are found in the eye disc. In these discs R cell axons project into the optic stalk, but are highly disorganised.

Flies expressing ttkp69.Scer\UAS under the control of Scer\GAL4cb12, Scer\GAL4cb19, Scer\GAL4cb35, Scer\GAL4cb36, Scer\GAL4cb37 or Scer\GAL4cb38 are moderately viable. Flies expressing ttkp69.Scer\UAS under the control of Scer\GAL4cb05 or Scer\GAL4cb23 are viable at 18oC.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4ey.PB results in deletion of the eye.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4dpp.blk1 affects furrow reincarnation but not birth in the eye disc.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4Eq1 results in a lack of most microchaetae.

Scer\GAL4Kr.PM-mediated expression leads to a partial block of neuronal development in the PNS and CNS. Scer\GAL4sca-537.4-mediated expression impairs neuronal differentiation. In late stage 16/17 embryos cell death is observed.

Eyes are deformed, severely reduced in size and lack bristles, cone cells and photoreceptor cells in flies expressing ttkp69.Scer\UAS under the control of Scer\GAL4GMR.PF.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Over-expression of lzGMR.PD in flies expressing ttkp69.Scer\UAS in under the control of Scer\GAL4GMR.PF partially suppresses the loss cone cells and R1, R6 and R7 photoreceptor cells.

Co-expression of repoScer\UAS.cYa and ttkp69.Scer\UAS under the control of Scer\GAL4sca-T3 results in a synergistic suppression of neuronal development in embryos.

Most notal microchaetae are missing in animals coexpressing phylScer\UAS.cPa and ttkp69.Scer\UAS under the control of Scer\GAL4Eq1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4twi.PB and Scer\GAL4how-24B partially rescues the muscle morphology defects of ttkD2-50 mutants.

Expression of ttkp69.Scer\UAS under the control of Scer\GAL4btl.PS in ttkD2-50 mutant animals rescues the tracheal intercalation defects. Terminal branch fusion defects of the lateral trunk are also rescued in these animals.

Short heat shocks and moderate temperatures in ttktwp/ttktwp, ttkp69.Scer\UAS Scer\GAL4hs.PB animals lead to significant partial rescue of dorsal appendage and chorion defects. Eggs are normal in shape and size and dorsal appendages are significantly longer than seen in the mutants alone. Longer heat shocks lead to a dramatic decline in rescue frequency accompanied by an increase in defects early in oogenesis.

Scer\GAL4sli.PS-mediated expression partially rescues the CNS phenotype with two copies of the construct.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ttkp69.Scer\UAS
ttkp69.UAS
Name Synonyms
Secondary FlyBase IDs
    References (19)