Adulthood-specific expression of ttkp69.UAS under the control of Scer\GAL4GMR44E10 leads to females with a severely decreased egg-laying rate and significant retention of mature follicles in the ovary, as compared to controls. Consistently, follicles rupture much less frequently upon induction by OA or ionomycin, as compared to controls.
Expression of ttkp69.Scer\UAS under the control of Scer\GAL4twi.PB and Scer\GAL4how-24B leads to lethality at embryonic stages due to severe defects in the specification of all three muscle types. The heart is either completely absent or only a few isolated cardioblasts are visible, visceral musculature contains gaps and has abnormal organization, somatic musculature contains very few correctly specific and differentiated muscle fibers, with cells that remain mononucleated and form small muscles that lack clear identity.
Expression of ttkp69.Scer\UAS under the control of Scer\GAL4lz-gal4 results in severely disrupted adult eyes that show scarring and lack ommatidial structures. In third instar larval eye discs ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells.
Expression of ttkp69.Scer\UAS under the control of Scer\GAL4GMR.PF results in severely perturbed adult eyes, displaying no ommatidial structure. Ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells.
When expression is driven by Scer\GAL4c739, ttkp69.Scer\UAS shows a mushroom body Class I phenotype; neuron number reduced. This phenotype is similar to, but less extreme than, that caused by ttkUY181 driven by Scer\GAL4c739. Loss of neurons is not accompanied by increase in number of glial cells.
When Scer\GAL4c527 is expressed under the control of Scer\GAL4c527 in third instar larvae, defects are seen in glial cell migration in the developing eye. In about 20% of these animals surface glial cells do not enter the eye disc and R cell axons fail to enter the optic stalk forming a mass of R cell axons in the basal region of the disc. In the remaining larvae, fewer surface glial cells are found in the eye disc. In these discs R cell axons project into the optic stalk, but are highly disorganised.
Scer\GAL4Kr.PM-mediated expression leads to a partial block of neuronal development in the PNS and CNS. Scer\GAL4sca-537.4-mediated expression impairs neuronal differentiation. In late stage 16/17 embryos cell death is observed.
Eyes are deformed, severely reduced in size and lack bristles, cone cells and photoreceptor cells in flies expressing ttkp69.Scer\UAS under the control of Scer\GAL4GMR.PF.