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General Information
Symbol
Dmel\sdtXP
Species
D. melanogaster
Name
FlyBase ID
FBal0060898
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sdtXP96
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    C8236040T

    Reported nucleotide change:

    C?T

    Amino acid change:

    R682term | sdt-PB; R269term | sdt-PD; R757term | sdt-PE; R249term | sdt-PF; R1410term | sdt-PG; R324term | sdt-PH; R659term | sdt-PI; R898term | sdt-PJ; R269term | sdt-PK; R1312term | sdt-PL; R1291term | sdt-PM; R682term | sdt-PN; R996term | sdt-PO; R563term | sdt-PP

    Reported amino acid change:

    R682term

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Nucleotide substitution: C?T.

    Amino acid replacement: R682term.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    sdtXP mutant clones in genetically mosaic eye discs do not show a growth advantage compared to wild-type clones.

    Embryos hemizygous for sdtXP show severely reduced cuticle production, typically resulting in embryos with fragmented cuticle.

    Homozygous eye clones results in bulky and irregularly shaped rhabdomeres. When exposed to light for 5 days, nearly all mutant photoreceptor cells survive.

    Homozygous clones can cover large areas of the wing without any overt phenotype when abutting the dorsoventral boundary or when running along the longitudinal veins.

    sdtXP somatic clones in the early pupal eye (38% pupal development), zonula adherens structure is only mildly disrupted. These defects are no longer detectable by late pupal stages, or in the adult eye. Stalk membranes in adult sdtXP mutant photoreceptors are reduced by 40% compared with the wild type, and are often smoother without the deep folds seen in wild-type. sdtXP mutant rhabdomeres become irregularly shaped, frequently expanded, split, or in contact with neighboring rhabdomeres. The base membrane of microvilli in these rhabdomeres is severely disrupted by vesicle-like membrane structures concentrating around the base of the rhabdomere.

    Marker analysis of photoreceptor cells in somatic clones of sdtXP in pupal eyes (40-50% pupal development) indicates displacement/expansion of the adherens junction and associated F-actin baso-laterally from the apical position it occupies in wild-type. The apical domains of these photoreceptors fail to orient to the center of ommatidia, resulting in the formation of rhabdomeres in displaced positions. Rhabdomeres in these clones are also often fragmented: longitudinal sections reveal discontinuous, disrupted rhabdomeres. The most severe disruption/loss of rhabdomeres is seen proximally, whereas distally rhabdomeres are relatively well organised. In the distal region of adult eyes rhabdomeres in somatic clones of sdtXP mutant cells are bulky and fused.

    In sdtXP germline clone embryos, an irregular cell shape and multilayered epithelial structure is seen at stages 11 to 12.

    Homozygous embryos derived from germ line clones exhibit severe malformations or an absence of epidermis. They also exhibit abnormal zonula adherens formation and mislocalisation of membrane protein neurotactin.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    NOT Enhancer of
    Statement
    Reference

    sdtXP/sdt[+] is a non-enhancer of ommatidium phenotype of Vangstbm-153

    NOT Suppressor of
    Statement
    Reference

    sdtXP/sdt[+] is a non-suppressor of ommatidium phenotype of Vangstbm-153

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Heterozygosity for sdtXP has no effect on the frequency of ommatidia that show planar cell polarity defects in Vangstbm-153 homozygotes.

    baz4 sdtXP follicle cell clones expressing bazS151A.S1085A.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4e22c lose their epithelial organisation and form multilayers of cells.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    References (14)