Allele Dmel\pum⁰⁶⁸⁹⁷

General Information
Symbol	Dmel\pum⁰⁶⁸⁹⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0060925
Feature type	allele	Associated gene	Dmel\pum
Also Known As	pum⁶⁸⁹⁷

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity (Lin and Spradling, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:4,983,316..4,983,316 (Lin and Spradling, 1997, Parisi and Lin, 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The P{lacW} transposon in inserted between exons 8 and 9 on the pum transcription unit. (Schweers et al., 2002) P{PZ} insertion in the intron between exons 8 and 9. The orientation of the P{PZ} element is such that the ry marker gene is towards the 5' side of the pum gene while the Ecol\lacZ gene is oriented towards the 3' side. (Parisi and Lin, 1999)
Caused by insertion	P{PZ}pum⁰⁶⁸⁹⁷ (Lin and Spradling, 1997, Parisi and Lin, 1999)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Lin and Spradling, 1997)
Phenotype Manifest In
	female germline stem cell \| larval stage (Parisi and Lin, 1999) ovariole (Parisi and Lin, 1999) ovary (Parisi and Lin, 1999, Lin and Spradling, 1997)
Detailed Description
	Statement Reference More than 50% of homozygous larvae survive the pupal stage to eclosion. 54% of homozygous ovaries from third instar larvae have an underproliferated germline, 23% have a normal number of germline cells and 23% have an overproliferated germline. The germ cells are grossly abnormal in morphology and size and the ovary is much larger than in wild-type larvae. By the midpupal stage, individual germaria have formed (as in wild type) but 63% of them do not contain germline cells and the remaining mutant ovarioles contain a small number of germline cells. By the adult stage, 86% of homozygous females have germlineless ovaries, 7% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 7% of females have one germlineless and one differentiated ovary. (Parisi and Lin, 1999) Small ovary phenotype. (Lin and Spradling, 1997)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	pum⁰⁷⁰⁹⁸ and pum⁰⁶⁸⁹⁷ have identical P{PZ} insertion sites. (Parisi and Lin, 1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	fs(3)06897 pum⁶⁸⁹⁷ (Parisi and Lin, 1999, Lin and Spradling, 1997, ) pum⁰⁶⁸⁹⁷ pum^ovt (Schweers et al., 2002)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Schweers et al., 2002, Genetics 161(3): 1177--1185 The Drosophila melanogaster translational repressor pumilio regulates neuronal excitability. [FBrf0151333] Parisi and Lin, 1999, Genetics 153(1): 235--250 The Drosophila pumilio gene encodes two functional protein isoforms that play multiple roles in germline development, gonadogenesis, oogenesis and embryogenesis. [FBrf0111454] Lin and Spradling, 1997, Development 124(12): 2463--2476 A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary. [FBrf0095585]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032]

Allele Dmel\pum06897

Allele Dmel\pum⁰⁶⁸⁹⁷