|Feature type||allele||Associated gene||Dmel\pum|
|Also Known As||pum2003|
|Map ( GBrowse )||
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Caused by insertion|
|Phenotype Manifest In|
Mutants exhibit increased long term facilitation (LTF) at the neuromuscular junction.
Homozygous females do not lay eggs. Approximately 50% of homozygous larvae survive the pupal stage to eclosion. By the adult stage, 43% of homozygous females have germlineless ovaries, 32% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 25% of females have one germlineless and one differentiated ovary. Homozygous female germline clones induced at the third larval instar stage produce ovarioles that contain on average 4.2 +/- 2.0 mature egg chambers but no other germ cells. Females containing homozygous female germline clones achieve an oviposition efficiency of 56% (for absolute number of eggs laid by the females). These eggs (derived from females containing homozygous germline clones mated to homozygous males) are sometimes capable of developing into adulthood.
Small ovary phenotype. None of the germ cells appear to undergo asymmetric divisions. Ovarioles contain only two clusters of apparently undifferentiated germline cells. Some ovaries in newly eclosed females completely lack germline cells though individual ovarioles are visible, in other ovaries the ovarioles are not recognisable. A small number of ovarioles contain 2-3 normally developing egg chambers that eventually develop into morphologically normal mature oocytes. Preoogenic germline development is also aberrant, germline stem cells of late third instar larval ovaries have no obvious spectrosome organisation.
Females are not able to maintain a normal germarial structure: instead of retaining active stem cells each ovariole houses only one or two germ line derivatives. When the non-functional egg chambers leave the germarium they are not replaced, the germarium assumes the appearance of a thin tube. Stem cells cannot be maintained and instead differentiate into cystoblast-like cells.
|Phenotype Manifest In|
pum02003 bamΔ86/pum9 bamΔ86 double mutant ovaries have a complex phenotype that is distinct from that of either single mutant. A mixture of apparently undifferentiated cells and overtly polyploid cells is seen. In many cases, the polyploid chromosomes are thick and expanded like nurse cell chromosomes. Occasionally these pseudo-nurse cells are organised within an epithelial layer of follicle cells, like a cyst, although these cysts never contain a full complement of 16 cystocytes. These cysts contain ring canals.
|Complementation & Rescue Data|
|Partially rescued by|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 7 )|
|Secondary FlyBase IDs|
|References ( 11 )|
|Personal communication to FlyBase|