Allele Dmel\pum⁰²⁰⁰³

General Information
Symbol	Dmel\pum⁰²⁰⁰³	Species	D. melanogaster
Name		FlyBase ID	FBal0060928
Feature type	allele	Associated gene	Dmel\pum
Also Known As	pum²⁰⁰³

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity (Lin and Spradling, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:4,983,704..4,983,704 (Lin and Spradling, 1997, Spradling, 1999.12.9, Parisi and Lin, 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The P{lacW} transposon in inserted between exons 8 and 9 on the pum transcription unit. (Schweers et al., 2002) P{PZ} insertion in the intron between exons 8 and 9. The orientation of the P{PZ} element is such that the ry marker gene is towards the 5' side of the pum gene while the Ecol\lacZ gene is oriented towards the 3' side. (Parisi and Lin, 1999)
Caused by insertion	P{PZ}pum⁰²⁰⁰³ (Lin and Spradling, 1997, Spradling, 1999.12.9, Parisi and Lin, 1999)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Spradling et al., 1997, Lin and Spradling, 1997) neurophysiology defective (Schweers et al., 2002) sterile (with pum¹) (Ye et al., 2004)
Phenotype Manifest In
	female germline stem cell (Spradling et al., 1997, Lin and Spradling, 1997) ovariole \| germline clone (Parisi and Lin, 1999) ovary (Parisi and Lin, 1999, Lin and Spradling, 1997) spectrosome (Lin and Spradling, 1997)
Detailed Description
	Statement Reference Mutants exhibit increased long term facilitation (LTF) at the neuromuscular junction. (Schweers et al., 2002) Homozygous females do not lay eggs. Approximately 50% of homozygous larvae survive the pupal stage to eclosion. By the adult stage, 43% of homozygous females have germlineless ovaries, 32% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 25% of females have one germlineless and one differentiated ovary. Homozygous female germline clones induced at the third larval instar stage produce ovarioles that contain on average 4.2 +/- 2.0 mature egg chambers but no other germ cells. Females containing homozygous female germline clones achieve an oviposition efficiency of 56% (for absolute number of eggs laid by the females). These eggs (derived from females containing homozygous germline clones mated to homozygous males) are sometimes capable of developing into adulthood. (Parisi and Lin, 1999) Small ovary phenotype. None of the germ cells appear to undergo asymmetric divisions. Ovarioles contain only two clusters of apparently undifferentiated germline cells. Some ovaries in newly eclosed females completely lack germline cells though individual ovarioles are visible, in other ovaries the ovarioles are not recognisable. A small number of ovarioles contain 2-3 normally developing egg chambers that eventually develop into morphologically normal mature oocytes. Preoogenic germline development is also aberrant, germline stem cells of late third instar larval ovaries have no obvious spectrosome organisation. (Lin and Spradling, 1997) Females are not able to maintain a normal germarial structure: instead of retaining active stem cells each ovariole houses only one or two germ line derivatives. When the non-functional egg chambers leave the germarium they are not replaced, the germarium assumes the appearance of a thin tube. Stem cells cannot be maintained and instead differentiate into cystoblast-like cells. (Spradling et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement Reference bam^Δ86, pum⁹/pum⁰²⁰⁰³ has germline cell \| female phenotype (Chen and McKearin, 2005, Chen and McKearin, 2005) bam^Δ86, pum⁹/pum⁰²⁰⁰³ has ovary phenotype (Chen and McKearin, 2005, Chen and McKearin, 2005)
Additional Comments
Genetic Interactions
Statement Reference pum⁰²⁰⁰³ bam^Δ86/pum⁹ bam^Δ86 double mutant ovaries have a complex phenotype that is distinct from that of either single mutant. A mixture of apparently undifferentiated cells and overtly polyploid cells is seen. In many cases, the polyploid chromosomes are thick and expanded like nurse cell chromosomes. Occasionally these pseudo-nurse cells are organised within an epithelial layer of follicle cells, like a cyst, although these cysts never contain a full complement of 16 cystocytes. These cysts contain ring canals. (Chen and McKearin, 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially complements	pum⁰¹⁶⁸⁸ (Parisi and Lin, 1999)
Partially rescued by	pum⁰²⁰⁰³ is partially rescued by pum^nos.PB (Parisi and Lin, 1999)
Comments	pum⁰¹⁶⁸⁸ partially complements pum⁰²⁰⁰³ for oogenic defects and embryonic lethality. (Parisi and Lin, 1999)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Strong ovarette class mutation. P{PZ} mobilisation demonstrates the insertion is responsible for the mutant phenotype. (Lin and Spradling, 1997)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	fs(3)02003 (Spradling, 1999.12.9, ) ovt (Lin, 1997) ovt¹ (Lin and Spradling, 1997) pum²⁰⁰³ (Chen and McKearin, 2005, Szakmary et al., 2005, Ye et al., 2004, Schweers et al., 2002, Parisi and Lin, 1999, ) pum⁰²⁰⁰³ pum^ovarette (Spradling et al., 1997) pum^ovt (Schweers et al., 2002)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Chen and McKearin, 2005, Curr. Biol. 15(2): 179--184 Gene circuitry controlling a stem cell niche. [FBrf0183822] Szakmary et al., 2005, Curr. Biol. 15(2): 171--178 Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation. [FBrf0183821] Ye et al., 2004, Curr. Biol. 14(4): 314--321 Nanos and Pumilio are essential for dendrite morphogenesis in Drosophila peripheral neurons. [FBrf0174478] Schweers et al., 2002, Genetics 161(3): 1177--1185 The Drosophila melanogaster translational repressor pumilio regulates neuronal excitability. [FBrf0151333] Parisi and Lin, 1999, Genetics 153(1): 235--250 The Drosophila pumilio gene encodes two functional protein isoforms that play multiple roles in germline development, gonadogenesis, oogenesis and embryogenesis. [FBrf0111454] Lin and Spradling, 1997, Development 124(12): 2463--2476 A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary. [FBrf0095585] Spradling et al., 1997, Cold Spring Harbor Symp. Quant. Biol. 62: 25--34 The Drosophila germarium: stem cells, germ line cysts, and oocytes. [FBrf0102402]
Review	Lin, 1997, A. Rev. Genet. 31: 455--491 The tao of stem cells in the germline. [FBrf0100666]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Spradling, 1999.12.9, Helping FlyBase with P-element paper. Helping FlyBase with P-element paper. [FBrf0125024] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\pum02003

Allele Dmel\pum⁰²⁰⁰³