FlyBase Allele Report: Dmel\piwi[06843]

General Information

Symbol

Dmel\piwi⁰⁶⁸⁴³

Species

D. melanogaster

Name

FlyBase ID

FBal0060944

Feature type

allele

Associated gene

Dmel\piwi

Associated Insertion(s)

P{PZ}piwi⁰⁶⁸⁴³

Carried in Construct

Also Known As

piwi³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Lin and Spradling, 1997)

Progenitor genotype

Associated Insertion(s)

P{PZ}piwi⁰⁶⁸⁴³

Cytology

Description

P{PZ} is inserted in the first exon. The orientation of the P{PZ} is opposite to that of the gene.

(Cox et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:10,987,117..10,987,453

(De Donno et al., 2023, Jin et al., 2013, Sofola et al., 2007, Kalmykova et al., 2005, Spradling et al., 1999, Cox et al., 1998, Lin and Spradling, 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis (with piwi²)

(Yang et al., 2019)

abnormal mitotic cell cycle | germline clone

(Cox et al., 1998)

abnormal size | oogenesis (with piwi²)

(Yang et al., 2019)

abnormal size | oogenesis | recessive

(Yang et al., 2019)

decreased fecundity | female (with piwi²)

(Yang et al., 2019)

female sterile

(Lin and Spradling, 1997)

female sterile (with piwi²)

(Yang et al., 2019)

female sterile | recessive

(Spradling et al., 1999)

neoplasia | oogenesis (with piwi²)

(Yang et al., 2019)

Phenotype Manifest In

cystoblast (with piwi²)

(Yang et al., 2019)

egg chamber (with Df(2L)BSC145)

(Atikukke et al., 2014)

embryo | embryonic stage 4 | germline clone

(Cox et al., 1998)

embryo | germline clone

(Cox et al., 1998)

embryonic head | germline clone

(Cox et al., 1998)

female germline stem cell

(Cox et al., 1998)

female germline stem cell (with piwi²)

(Yang et al., 2019)

germarium

(Cox et al., 1998)

ovariole

(Cox et al., 1998)

ovary

(Yang et al., 2019)

ovary (with piwi²)

(Yang et al., 2019)

Detailed Description

Statement

Reference

piwi⁰⁶⁸⁴³ homozygous and piwi²/piwi⁰⁶⁸⁴³ transheterozygous females exhibit rudimentary ovaries.

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/Df(2L)BSC145 mutant ovaries frequently contain agametic germaria and lack egg chambers. Only one to two egg chambers is produced per ovariole and occasional compound egg chambers (multiple cysts in one follicular epithelium) is seen.

(Atikukke et al., 2014)

Mutant ovaries show normal morphology at the third larval instar. Germ-line stem cells are normal in number and correctly positioned in third instar ovaries. Terminal filament formation is normal. Adult ovaries contain only 2 egg chambers connected to a germarium lacking germ line cells. Embryos derived from germ-line clones have various defects, including mitotic defects during cleavage stage or later severe deformation of head region.

(Cox et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³ has abnormal size | oogenesis phenotype, suppressible | partially by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has abnormal size | oogenesis phenotype, suppressible | partially by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

NOT suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³/piwi² has abnormal cell number | oogenesis phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has neoplasia | oogenesis phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has decreased fecundity | female phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has female sterile phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

Enhancer of

Statement

Reference

piwi⁰⁶⁸⁴³/piwi[+] is an enhancer of abnormal meiotic cell cycle phenotype of RanGAP^Sd

(Gell and Reenan, 2013)

NOT Enhancer of

Statement

Reference

piwi⁰⁶⁸⁴³/piwi[+] is a non-enhancer of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

NOT Suppressor of

Statement

Reference

piwi⁰⁶⁸⁴³/piwi[+] is a non-suppressor of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

piwi⁰⁶⁸⁴³ is a non-suppressor of abnormal eye color phenotype of w^RNAi.GMR

(Pelisson et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(2L)BSC145/piwi⁰⁶⁸⁴³ has egg chamber phenotype, enhanceable by armi^+t6.3/Df(3L)Exel6095/CycJ^null

(Atikukke et al., 2014)

NOT Enhanced by

Statement

Reference

Df(2L)BSC145/piwi⁰⁶⁸⁴³ has egg chamber phenotype, non-enhanceable by CycJ^+t4/armi^+t6.3/Df(3L)Exel6095/CycJ^null

(Atikukke et al., 2014)

Suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³/piwi² has ovary phenotype, suppressible | partially by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³ has ovary phenotype, suppressible | partially by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

NOT suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³/piwi² has female germline stem cell phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has cystoblast phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

NOT Suppressor of

Statement

Reference

piwi⁰⁶⁸⁴³/piwi⁰⁶⁸⁴³ is a non-suppressor of eye phenotype of w^RNAi.GMR

(Pelisson et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

A CycJ^null/Df(3L)Exel6095 mutant background (expressing armi^+t6.3 to restore armi[+] function) enhances the oogenesis defects seen in piwi⁰⁶⁸⁴³/Df(2L)BSC145 mutant females. The frequency of compound egg chambers (multiple cysts in one follicular epithelium) is comparable to that seen in Df(3L)armi-J. Addition of CycJ^+t4 decreases the frequency of compound egg chambers to the levels seen in the piwi mutant alone.

(Atikukke et al., 2014)

The segregation distortion seen in males carrying either the SD-5star or SD-LosArenos Dp(2;2)RanGAP^SD chromosome variant (in a sensitive Rsp background) is enhanced (the k[[c]] value, or proportion of Dp(2;2)RanGAP^SD-bearing progeny as the fraction of total progeny (corrected for viability), is significantly increased) if the males are also carrying a single copy of piwi⁰⁶⁸⁴³.

(Gell and Reenan, 2013)

The survival of roX1^ex33A Df(1)roX2Δ adult males is not significantly altered if they are also heterozygous for piwi⁰⁶⁸⁴³.

(Menon and Meller, 2012)

The yellowish eye colour caused by expression of one copy of w^dsRNA.GMR in a wild-type background is not altered if the flies are also homozygous for piwi⁰⁶⁸⁴³.

(Pelisson et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

piwi⁰⁶⁸⁴³/piwi² is rescued by Scer\GAL4^C587/piwi^UASp.cYa

(Yang et al., 2019)

piwi⁰⁶⁸⁴³ is rescued by Scer\GAL4^C587/piwi^UASp.cYa

(Yang et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

12225

P{PZ}piwi⁰⁶⁸⁴³ cn¹/CyO; ry⁵⁰⁶

Kyoto

108882

P{PZ}piwi⁰⁶⁸⁴³ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

Comments

Mobilisation of the P{PZ} insertion reverts the mutant phenotype.

(Lin and Spradling, 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

PIWI³

(Dufourt et al., 2011)

fs(2)06843

(Spradling et al., 1999)

piwi⁰⁶⁸⁴³

(De Donno et al., 2023, Basu et al., 2016, Atikukke et al., 2014, Hayashi et al., 2014, Gell and Reenan, 2013, Menon and Meller, 2012, Sofola et al., 2007, Christensen and Cook, 2006.8.30)

piwi³

(Stolyarenko, 2020, Kotov et al., 2019, Yang et al., 2019, Klenov et al., 2011, Shpiz et al., 2011, Shpiz et al., 2009, Desset et al., 2008, Pelisson et al., 2007, Kalmykova et al., 2005, Cox et al., 1998)

piwi⁶⁸⁴³

(Lin and Spradling, 1997)

piwi^pz06843

(Jin et al., 2013)

piwi^pz

(Jin et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (25)

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