FlyBase Allele Report: Dmel\piwi[2]

piwi²/piwi⁰⁶⁸⁴³ transheterozygous females exhibit rudimentary ovaries, germaria are frequently full of germline stem cell-like cells ("tumorous"), and no mature eggs are produced.

(Yang et al., 2019)

Egg chambers of piwi^Nt/piwi² females have a near-normal number of egg chambers in the ovarioles.

(Klenov et al., 2011)

piwi¹/piwi² ovaries show abnormal cell death.

(Zamparini et al., 2011)

Eye colour: homozygotes have normal levels of eye pigmentation.

(Yin and Lin, 2007)

Suppressor of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon.

(Pal-Bhadra et al., 2004)

Homozygous females usually contain a rudimentary germarium and only a few egg chambers.

(King et al., 2001)

Homozygous germline stem cells (GSCs) (induced by mitotic recombination immediately prior to oogenesis) are present three weeks following oogenesis. The homozygous GSCs divide four-fold slower than their wild-type counterparts. Cysts and postgerminal egg chambers derived from these homozygous GSCs usually develop normally.

(Cox et al., 2000)

Mutant ovaries show normal morphology at the third larval instar. Germ-line stem cells are normal in number and correctly positioned in third instar ovaries. Terminal filament formation is normal. Adult ovaries contain only 2 egg chambers connected to a germarium lacking germ line cells. Embryos derived from germ-line clones are arrested in embryogenesis, and cannot be rescued by paternal piwi⁺. They have various defects, including mitotic defects during cleavage stage or later severe deformation of head region. Wild type germaria transplanted into the body cavity of piwi² females developed normally. Somatic clonal analysis reveals that piwi function is not required in follicle cells for egg chamber development.

(Cox et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³/piwi² has abnormal size | oogenesis phenotype, suppressible | partially by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi² has abnormal size | oogenesis phenotype, suppressible by hh^hs.PI

(King et al., 2001)

NOT suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³/piwi² has abnormal cell number | oogenesis phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has neoplasia | oogenesis phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has decreased fecundity | female phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has female sterile phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi² has female sterile phenotype, non-suppressible by hh^hs.PI

(King et al., 2001)

Enhancer of

Statement

Reference

piwi[+]/piwi² is an enhancer of visible phenotype of Kr^If-1

(Gangaraju et al., 2011)

Suppressor of

Statement

Reference

piwi²/piwi¹ is a suppressor of male semi-fertile | progressive phenotype of Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰

(Ku et al., 2016)

Other

Statement

Reference

Df(3R)ea/+, piwi² has male sterile | recessive phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)96B/+, piwi² has partially lethal - majority die phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)by10/+, piwi² has partially lethal - majority die phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)P115/+, piwi² has male sterile | recessive phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)ry27/+, piwi² has partially lethal - majority die phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)ry27/+, piwi² has lethal | female phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)ry506-85C/+, piwi² has lethal | female phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)2-2/+, piwi² has lethal phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)23D1/+, piwi² has lethal phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)p40/+, piwi² has lethal phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)p712/+, piwi² has lethal phenotype

(Smulders-Srinivasan and Lin, 2003)

+/Df(3R)C4, piwi² has male sterile | recessive phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)DG2/+, piwi² has partially lethal - majority die phenotype

(Smulders-Srinivasan and Lin, 2003)

Df(3R)DG2/+, piwi² has male sterile | recessive phenotype

(Smulders-Srinivasan and Lin, 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³/piwi² has ovary phenotype, suppressible | partially by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi²/piwi¹ has ovary phenotype, suppressible | partially by Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰

(Ku et al., 2016)

piwi² has female germline stem cell phenotype, suppressible by CG7816[+]/Zip99C³

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by Df(3L)66C-G28/+

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by Df(3L)Aprt-1

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by Df(3L)kto2/+

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by Df(3L)ri-79c/+

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by +/Df(3R)L127

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by Df(3R)faf-BP/+

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by P{wA}4-4/+

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by RpS3[+]/RpS3¹

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by Sry-δ¹²/Sry-delta[+]

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by Sry-δ^SF2/Sry-delta[+]

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by T(Y;3)B81/+

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by l(3)99Da[+]/l(3)99Da¹

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by l(3)99De¹/l(3)99De[+]

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by l(3)99Dg[+]/l(3)99Dg³

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by l(3)99Ea[+]/l(3)99Ea¹

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by l(3)99Ea[+]/l(3)99Ea⁴

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by sima[+]/sima^j11B7

(Smulders-Srinivasan and Lin, 2003)

piwi² has female germline stem cell phenotype, suppressible by tgo[+]/tgo⁵

(Smulders-Srinivasan and Lin, 2003)

piwi² has egg chamber phenotype, suppressible by hh^hs.PI

(King et al., 2001)

piwi² has germarium phenotype, suppressible by hh^hs.PI

(King et al., 2001)

NOT suppressed by

Statement

Reference

piwi⁰⁶⁸⁴³/piwi² has female germline stem cell phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi⁰⁶⁸⁴³/piwi² has cystoblast phenotype, non-suppressible by ova^{UASp.Tag:FLAG}/Scer\GAL4^C587

(Yang et al., 2019)

piwi² has female germline stem cell phenotype, non-suppressible by Sry-delta[+]/Sry-δ^SF1

(Smulders-Srinivasan and Lin, 2003)

Enhancer of

Statement

Reference

piwi[+]/piwi² is an enhancer of eye phenotype of Kr^If-1

(Gangaraju et al., 2011)

Suppressor of

Statement

Reference

piwi²/piwi¹ is a suppressor | partially of testis phenotype of Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰

(Ku et al., 2016)

piwi² is a suppressor of wing phenotype of sd^Fab-X

(Grimaud et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

piwi¹/+ (or even more so, piwi¹/piwi²) significantly partially suppresses the swollen apical tip of the testis phenotype seen in Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ males. piwi¹/piwi² suppresses infertility seen in Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ males. Tudor-SN⁰⁶¹⁴/Tudor-SN¹¹²⁰ partially suppresses ovary defects in piwi¹/piwi² females.

(Ku et al., 2016)

piwi²/+ enhances the eye outgrowth phenotype phenotype of Kr^If-1; approximately 7% of the progeny have an eye outgrowth phenotype in a cross of piwi²/+ females to Kr^If-1/+ males. However, the reciprocal cross of Kr^If-1/+ females to piwi²/+ males does not result in progeny with an eye outgrowth phenotype, indicating a maternal effect. In addition, the expression of the eye outgrowth phenotype depends on the presence of the piwi² mutation in the progeny, indicating a zygotic effect.

(Gangaraju et al., 2011)

piwi²/+ does not impair cytotype-mediated repression of P-element excision.

(Simmons et al., 2007)

The wing phenotype of sd^Fab-X flies is suppressed in sd^Fab-X;piwi² flies.

(Grimaud et al., 2006)

Over expression of hh^hs.PI in piwi² homozygotes or heterozygotes stimulates somatic cell differentiation in ovarioles. hh^hs.PI also restores germ stem cell divisions in these mutants. Rescued homozygotes often contain a full complement of germline cells. Moreover the germaria generate more egg chambers, per ovariole, similar to heterozygous controls. However fertility is not restored.

(King et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

piwi⁰⁶⁸⁴³/piwi² is rescued by Scer\GAL4^C587/piwi^UASp.cYa

(Yang et al., 2019)

piwi² is rescued by piwi^Tag:TAP

(Megosh et al., 2006)

Not rescued by

piwi²/piwi¹ is not rescued by piwi^YK.GFP

(Le Thomas et al., 2013)

Comments

Embryos derived from germ-line clones are arrested in embryogenesis, and cannot be rescued by paternal piwi⁺.

(Cox et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

43319

w¹¹¹⁸; P{ry11}piwi²/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

PIWI²

(Dufourt et al., 2011)

fs(2)ry5

piwi2

(Le Thomas et al., 2013)

piwi²

(Saha et al., 2023, Fefelova et al., 2022, Lim et al., 2022, Zhang et al., 2021, Fort-Aznar et al., 2020, Kordyukova et al., 2020, Lin et al., 2020, Stolyarenko, 2020, Kotov et al., 2019, Stein et al., 2019, Yang et al., 2019, Teixeira et al., 2017, Klein et al., 2016, Ku et al., 2016, Wang et al., 2015, Basquin et al., 2014, Minakhina et al., 2014, Wang et al., 2014, Dönertas et al., 2013, Jin et al., 2013, Li et al., 2013, Sienski et al., 2012, Simmons et al., 2012, Gangaraju et al., 2011, Klenov et al., 2011, Liu et al., 2011, Pek and Kai, 2011, Shpiz et al., 2011, Zamparini et al., 2011, Nagao et al., 2010, Olivieri et al., 2010, Simmons et al., 2010, Smulders-Srinivasan et al., 2010, Habayeb et al., 2009, Shpiz et al., 2009, Desset et al., 2008, Brower-Toland et al., 2007, Josse et al., 2007, Simmons et al., 2007, Yin and Lin, 2007, Grimaud et al., 2006, Megosh et al., 2006, Vagin et al., 2006, Kalmykova et al., 2005)

piwi^ry5

(Chen and McKearin, 2005)

Name Synonyms

Secondary FlyBase IDs

References (59)

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