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General Information
Symbol
Dmel\piwi2
Species
D. melanogaster
Name
FlyBase ID
FBal0060945
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{ry11} is inserted in exon 4 in the same orientation as the gene.

Insertion components
P{ry11}piwi2
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

piwi2/piwi06843 transheterozygous females exhibit rudimentary ovaries, germaria are frequently full of germline stem cell-like cells ("tumorous"), and no mature eggs are produced.

Egg chambers of piwiNt/piwi2 females have a near-normal number of egg chambers in the ovarioles.

piwi1/piwi2 ovaries show abnormal cell death.

Eye colour: homozygotes have normal levels of eye pigmentation.

Suppressor of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon.

Homozygous females usually contain a rudimentary germarium and only a few egg chambers.

Homozygous germline stem cells (GSCs) (induced by mitotic recombination immediately prior to oogenesis) are present three weeks following oogenesis. The homozygous GSCs divide four-fold slower than their wild-type counterparts. Cysts and postgerminal egg chambers derived from these homozygous GSCs usually develop normally.

Mutant ovaries show normal morphology at the third larval instar. Germ-line stem cells are normal in number and correctly positioned in third instar ovaries. Terminal filament formation is normal. Adult ovaries contain only 2 egg chambers connected to a germarium lacking germ line cells. Embryos derived from germ-line clones are arrested in embryogenesis, and cannot be rescued by paternal piwi+. They have various defects, including mitotic defects during cleavage stage or later severe deformation of head region. Wild type germaria transplanted into the body cavity of piwi2 females developed normally. Somatic clonal analysis reveals that piwi function is not required in follicle cells for egg chamber development.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference

piwi[+]/piwi2 is an enhancer of visible phenotype of KrIf-1

Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
Statement
Reference

piwi2 has egg chamber phenotype, suppressible by hhhs.PI

piwi2 has germarium phenotype, suppressible by hhhs.PI

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

piwi[+]/piwi2 is an enhancer of eye phenotype of KrIf-1

Suppressor of
Statement
Reference

piwi2 is a suppressor of wing phenotype of sdFab-X

Additional Comments
Genetic Interactions
Statement
Reference

piwi1/+ (or even more so, piwi1/piwi2) significantly partially suppresses the swollen apical tip of the testis phenotype seen in Tudor-SN0614/Tudor-SN1120 males. piwi1/piwi2 suppresses infertility seen in Tudor-SN0614/Tudor-SN1120 males. Tudor-SN0614/Tudor-SN1120 partially suppresses ovary defects in piwi1/piwi2 females.

piwi2/+ enhances the eye outgrowth phenotype phenotype of KrIf-1; approximately 7% of the progeny have an eye outgrowth phenotype in a cross of piwi2/+ females to KrIf-1/+ males. However, the reciprocal cross of KrIf-1/+ females to piwi2/+ males does not result in progeny with an eye outgrowth phenotype, indicating a maternal effect. In addition, the expression of the eye outgrowth phenotype depends on the presence of the piwi2 mutation in the progeny, indicating a zygotic effect.

piwi2/+ does not impair cytotype-mediated repression of P-element excision.

The wing phenotype of sdFab-X flies is suppressed in sdFab-X;piwi2 flies.

Over expression of hhhs.PI in piwi2 homozygotes or heterozygotes stimulates somatic cell differentiation in ovarioles. hhhs.PI also restores germ stem cell divisions in these mutants. Rescued homozygotes often contain a full complement of germline cells. Moreover the germaria generate more egg chambers, per ovariole, similar to heterozygous controls. However fertility is not restored.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments

Embryos derived from germ-line clones are arrested in embryogenesis, and cannot be rescued by paternal piwi+.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (49)