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General Information
Symbol
Dmel\pbRev3.HSPB
Species
D. melanogaster
Name
FlyBase ID
FBal0060955
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
HSPBRev3
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G6711911A

Reported nucleotide change:

G118A

Amino acid change:

R202H | pb-PA; R197H | pb-PB; R197H | pb-PC; R192H | pb-PD

Reported amino acid change:

R202H

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: R?H. The amino acid replacement is at position 5 with respect to the beginning of the homeobox.

Nucleotide substitution: G118A. Amino acid replacement: R202H. In exon 4.

Point mutation (CGC to CAC), changes the Arg at the N terminus (5th residue) of the homeodomain to His.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

2 copies of pbRev3.HSPB leads to flies with a slightly reduced eye. Four copies leads to a complete deletion of the eye.

Flies carrying pbRev3.HSPB show eye loss.

Flies carrying one copy of pbRev3.HSPB are wild type. Flies carrying two copies of pbRev3.HSPB have a single ectopic fine bristle on antennal segment 3. Heat shock causes marked reductions in the eye in flies carrying two copies of pbRev3.HSPB.

Flies appear wild type in the absence of heat shock. One copy of the transgene causes no visible phenotype. Increasing the copies to four causes reduction in eye tissue until flies have no eyes and antenna take on a more rectangular form carrying a single fine bristle.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

pbRev3.HSPB has visible phenotype, enhanceable by ey[+]/eyD1Da

pbRev3.HSPB has visible phenotype, enhanceable by ey[+]/eyJD

Phenotype Manifest In
Enhanced by
Statement
Reference

pbRev3.HSPB has eye phenotype, enhanceable by eyJD

pbRev3.HSPB has eye phenotype, enhanceable by eygM3-12

pbRev3.HSPB has eye phenotype, enhanceable by Df(4)BA

pbRev3.HSPB has eye phenotype, enhanceable by ey2

pbRev3.HSPB has eye phenotype, enhanceable by eyD1Da

pbRev3.HSPB has eye phenotype, enhanceable by eyEH

pbRev3.HSPB has eye phenotype, enhanceable by eyl1

pbRev3.HSPB has eye phenotype, enhanceable by ey[+]/eyD1Da

pbRev3.HSPB has eye phenotype, enhanceable by ey[+]/eyJD

NOT Enhanced by
Statement
Reference

pbRev3.HSPB has eye phenotype, non-enhanceable by eya2

pbRev3.HSPB has eye phenotype, non-enhanceable by eyacli-IID

pbRev3.HSPB has eye phenotype, non-enhanceable by eygc1

pbRev3.HSPB has eye phenotype, non-enhanceable by so1

pbRev3.HSPB has eye phenotype, non-enhanceable by soL31

Other
Additional Comments
Genetic Interactions
Statement
Reference

The combination of 2 copies of eyJD with pbRev3.HSPB often leads to flies with complete eye loss and the fusion of the antenna and maxillary palp. In some cases, the maxillary palp, remains adjoined to the antennal appendage. However differentiation od the proboscis is not affected. The addition of eyEH, or eyD1Da to flies with 2 copies of pbRev3.HSPB, leads to a strong enhancement of the eye loss phenotype. The addition of ey2, or Df(4)BA to flies with 2 copies of pbRev3.HSPB, leads to an enhancement of the eye loss phenotype. The addition of eyl1, or eygM3-12 to flies with 2 copies of pbRev3.HSPB, leads to a weak enhancement of the eye loss phenotype. The addition of so1, soL31, eya2, eyacli-IID or eygc1 to flies with 2 copies of pbRev3.HSPB, does not enhance the eye loss phenotype.

eyJD and eyD1Da dominantly enhance the eye loss phenotype of pbRev3.HSPB flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to rescue
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Loss of function allele in the mouthparts and aristae and gain of function in the proximal antenna and eyes.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (5)