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General Information
Symbol
Dmel\msn102
Species
D. melanogaster
Name
FlyBase ID
FBal0061022
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Inversion breakpoint within the coding region.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

eye photoreceptor cell & axon | somatic clone

photoreceptor & axon, with Scer\GAL469B, msnP656A,P659A.UAS

photoreceptor & axon | somatic clone

photoreceptor cell | precursor & nucleus | somatic clone

photoreceptor cell R1 & axon (with msn03349)

photoreceptor cell R1 & axon | somatic clone

photoreceptor cell R2 & axon (with msn03349)

photoreceptor cell R2 & axon | somatic clone

photoreceptor cell R3 & axon (with msn03349)

photoreceptor cell R3 & axon | somatic clone

photoreceptor cell R4 & axon (with msn03349)

photoreceptor cell R4 & axon | somatic clone

photoreceptor cell R5 & axon (with msn03349)

photoreceptor cell R5 & axon | somatic clone

photoreceptor cell R6 & axon (with msn03349)

photoreceptor cell R6 & axon | somatic clone

Detailed Description
Statement
Reference

Females carrying homozygous follicle cell clones can produce round eggs.

When somatic clones are made in the developing eye, man differentiated R-cell precursor nuclei are localised abnormally at the basal region. This appears to reflect a failure for R-cell precursor nuclei to migrate along the apical-basal axis. About 60% of nuclei exhibit this phenotype. When the size of mutant clone patches exceeds 40 ommatidia, a furrow like structure is often formed within the clone. This was always positioned in parallel to the morphogenetic furrow.

Approximately 30% of msn102 homozygous embryos shows a 'dorsal open' phenotype.

Many R1-R6 growth cones fail to stop at the lamina layer in msn03349/msn102 larvae.

Somatic clones in the eye leads to pathfinding defects in the photoreceptor axons. While photoreceptor axons derived from clones extend normally into the brain, the R1 to R6 axons fail to elaborate a smooth retinotopic array in the lamina. Many R1 ro R6 axons terminate prematurely giving rise to an uneven lamina neuropil. Surprisingly the growth cones that innervate the medulla appear to expand normally, although their projections are somewhat disorganised. msnP656A,P659A.Scer\UAS when driven by Scer\GAL469B in a msn102 background produces third instar larvae with severe defects in axonal targeting. On leaving the optic stalk axons from photoreceptors form very large axonal bundles and fail to defasciculate in the region of either the lamina or the medulla.

In msn102 somatic clones in the eye ommatidia often have malformed or missing photoreceptors. Those ommatidia that have the normal complement of photoreceptors are often misrotated and display the wrong chiral form or are symmetrical. In mosaic photoreceptor R3/R4 pairs, the msn+ photoreceptor has a strong preference for adopting the R3 fate. This can often lead to chirality inversions where the msn+ R4 precursor adopts the R3 position displacing the msn102 R3 precursor. The mosaic analysis also showed that for correct 90o rotation there is no bias for any particular photoreceptor.

Homozygous clones in the developing eye result in defects in photoreceptor cell innervation; the lamina appears uneven and abnormal, large bundles of axons are seen in the medulla. R1-R6 photoreceptor cell axons from mutant clones pass over the lamina and terminate abnormally in the medulla.

Homozygous or msn102/msn172 embryos show defects in dorsal closure.

Clones in the eye are externally rough. They contain elongated rhabdomeres and some ommatidia also contain missing or an extra photoreceptor.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Suppressor of
Statement
Reference

msn[+]/msn102 is a suppressor | partially of visible phenotype of Rala35d

msn[+]/msn102 is a suppressor of visible phenotype of Rac1GMR.PN

msn[+]/msn102 is a suppressor of abnormal planar polarity phenotype of dshhs.sev.B

NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
Enhancer of
Statement
Reference

msn102 is an enhancer of photoreceptor & axon phenotype of dock04723

NOT Enhancer of
Statement
Reference

msn[+]/msn102 is a non-enhancer of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053

msn[+]/msn102 is a non-enhancer of ommatidium phenotype of MtlUAS.cMa, Scer\GAL4hs.2sev

msn102 is a non-enhancer of photoreceptor cell R1 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-enhancer of photoreceptor cell R2 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-enhancer of photoreceptor cell R3 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-enhancer of photoreceptor cell R4 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-enhancer of photoreceptor cell R5 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-enhancer of photoreceptor cell R6 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

Suppressor of
Statement
Reference

msn[+]/msn102 is a suppressor | partially of microchaeta phenotype of Rala35d

msn[+]/msn102 is a suppressor of macrochaeta phenotype of Rala35d

msn[+]/msn102 is a suppressor of eye phenotype of Rac1GMR.PN

msn[+]/msn102 is a suppressor of ommatidium phenotype of Rac1GMR.PN

msn[+]/msn102 is a suppressor of ommatidium phenotype of dshhs.sev.B

NOT Suppressor of
Statement
Reference

msn[+]/msn102 is a non-suppressor of wing phenotype of HIV-1\VpuUAS.cLa, Scer\GAL4dpp.blk1

msn[+]/msn102 is a non-suppressor of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053

msn[+]/msn102 is a non-suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa

msn[+]/msn102 is a non-suppressor of ommatidium phenotype of MtlUAS.cMa, Scer\GAL4hs.2sev

msn102 is a non-suppressor of photoreceptor cell R5 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-suppressor of photoreceptor cell R6 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-suppressor of photoreceptor cell R1 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-suppressor of photoreceptor cell R2 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-suppressor of photoreceptor cell R3 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

msn102 is a non-suppressor of photoreceptor cell R4 & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bifUAS.cRa

Other
Additional Comments
Genetic Interactions
Statement
Reference

msn102 does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; msn102 clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, the proportion of Df(1)su(s)R194/+ cells in the larval eye disc is not increased by the presence of msn102.

Introduction of a single copy of stck18 into the homozygous msn102 background reduces the incidence of a 'dorsal open' phenotype from approximately 30% to approximately 20%.

Homozygosity for icsBG02577 suppresses the msn102 'dorsal open' phenotype, reducing its incidence from approximately 30% to approximately 2%.

The photoreceptor projection phenotype caused by expression of bifScer\UAS.cRa under the control of Scer\GAL4GMR.PF is not modified by msn102.

msn102, suppresses the ommatidial polarity defects seen in dshhs.sev.B flies grown at 29oC. About 85% of ommatidia are in their correct polarity compared to 55% in dshhs.sev.B alone.

msn102/+ in double heterozygous combination with either bsk1/+, bsk2/+, bskflp147E/+, hep1/+ or hepr75/+ produces embryos with a dorsal open phenotype. The dorsal open phenotype of msn102/msn172 embryos is partially rescued by JraAsp.hs.sev expressed using heat shock or by tkvQ253D.Scer\UAS.cNb expressed under the control of Scer\GAL469B.

Xenogenetic Interactions
Statement
Reference

msn102/+ does not suppress the wing defects caused by expression of HIV-1\VpuScer\UAS.cLa under the control of Scer\GAL4dpp.blk1.

Complementation and Rescue Data
Partially rescued by
Not rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (22)