Allele Dmel\da^Scer\UAS.cGa

General Information
Symbol	Dmel\da^Scer\UAS.cGa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Giebel	FlyBase ID	FBal0061468
Feature type	allele	Associated gene	Dmel\da

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Giebel et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: 2.5kb BglII fragment of da coding region is cloned into pUAST vector. (Giebel et al., 1997)
Carried in construct	P{UAS-da.G} (Giebel, 1999, Wildonger and Mann, 2005, Castanon et al., 2001, Cadigan et al., 2002, Huang et al., 2000, Hoch and Jackle, 1998, Giebel et al., 1997, Jafar-Nejad et al., 2006, Bhattacharya and Baker, 2009, Salzer et al., 2010, Egoz-Matia et al., 2011, Park et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^da.G32 (Giebel et al., 1997)
Phenotype Manifest In
	amnioserosa, with Scer\GAL4^da.G32 (Giebel et al., 1997) commissure, with Scer\GAL4^da.G32 (Giebel et al., 1997) cuticle \| dorsal, with Scer\GAL4^da.G32 (Giebel et al., 1997) extended germ band embryo, with Scer\GAL4^da.G32 (Giebel et al., 1997) head, with Scer\GAL4^da.G32 (Giebel et al., 1997) longitudinal connective, with Scer\GAL4^da.G32 (Giebel et al., 1997) macrochaeta \| supernumerary, with Scer\GAL4^pnr-MD237 (Wildonger and Mann, 2005) presumptive embryonic/larval nervous system, with Scer\GAL4^da.G32 (Giebel et al., 1997) sensillum campaniformium of dorsal radius \| supernumerary, with Scer\GAL4^sca-109-68 (Jafar-Nejad et al., 2006) ventral nerve cord, with Scer\GAL4^da.G32 (Giebel et al., 1997)
Detailed Description
	Statement Reference The number of gl-positive corpora cardiaca precursor cells is normal in stage 12 embryos expressing da[Scer\UAS.cGa] under the control of Scer\GAL4[twi.2PE]. (Park et al., 2011) R7 development is not affected by da[Scer\UAS.cGa] expression, as judged from normal run and pros expression. (Bhattacharya and Baker, 2009) Overexpression of da^Scer\UAS.cGa, under the control of Scer\GAL4^sca-109-68, generates 17.1 extra sensory organs along the third wing vein compared to wild type wings. (Jafar-Nejad et al., 2006) Expression of da^Scer\UAS.cGa, under the control of Scer\GAL4^pnr-MD237, results in the presence of extra macrochaetae on the adult thorax. (Wildonger and Mann, 2005) Over expression of da^Scer\UAS.cGa, when driven by Scer\GAL4^twi.PB has little or no effect on mesoderm development. (Castanon et al., 2001) Expression of da^Scer\UAS.cGa under the control of Scer\GAL4^sca-537.4 has only a minor effect on the number of elav-positive neurons. (Huang et al., 2000) Scer\GAL4^da.G32-mediated embryonic lethality is due to extensive neural defects. Two copies of da^Scer\UAS.cGa cause a stronger phenotype: embryos exhibit incomplete germ band retraction, head involution and dorsal closure, defects in connectives and commissures are more frequent. (Giebel et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^GMR.PF, da^Scer\UAS.cGa, Scer\GAL4^GMR.PF is a suppressor \| partially of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, wg^{wg.3'UTR.Scer\UAS} (Cadigan et al., 2002)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^pnr-MD237, da^Scer\UAS.cGa has macrochaeta \| supernumerary phenotype, enhanceable by nvy^{dsRNA.Scer\UAS} (Wildonger and Mann, 2005) Scer\GAL4^sca-109-68, da^Scer\UAS.cGa has sensillum campaniformium of dorsal radius \| supernumerary phenotype, enhanceable by sens^Scer\UAS.cNa/Scer\GAL4^sca-109-68 (Jafar-Nejad et al., 2006)
Suppressed by
Statement Reference Scer\GAL4^Eq1, da^Scer\UAS.cGa has adult thoracic sensillum phenotype, suppressible \| partially by Df(1)260-1 (Jafar-Nejad et al., 2006) Scer\GAL4^pnr-MD237, da^Scer\UAS.cGa has macrochaeta \| supernumerary phenotype, suppressible \| partially by nvy^Scer\UAS.cWa (Wildonger and Mann, 2005)
Enhancer of
Statement Reference da^Scer\UAS.cGa/Scer\GAL4^sca-109-68 is an enhancer of sensillum campaniformium of dorsal radius \| supernumerary phenotype of Scer\GAL4^sca-109-68, sens^Scer\UAS.cNa (Jafar-Nejad et al., 2006)
Suppressor of
Statement Reference da^Scer\UAS.cGa is a suppressor \| partially of adult thorax \| dorsal & mechanosensory sensory organ phenotype of Scer\GAL4^pnr-MD237, nvy^Scer\UAS.cWa (Wildonger and Mann, 2005) Scer\GAL4^dpp.blk1/da^Scer\UAS.cGa is a suppressor \| partially of morphogenetic furrow phenotype of Scer\GAL4^dpp.blk1, wg^{wg.3'UTR.Scer\UAS} (Cadigan et al., 2002) Scer\GAL4^dpp.blk1/da^Scer\UAS.cGa is a suppressor of morphogenetic furrow phenotype of Scer\GAL4^dpp.blk1, arm^{S10.Scer\UAS.T:Hsap\MYC} (Cadigan et al., 2002) Scer\GAL4^GMR.PF, da^Scer\UAS.cGa, Scer\GAL4^GMR.PF is a suppressor \| partially of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, wg^{wg.3'UTR.Scer\UAS} (Cadigan et al., 2002) Scer\GAL4^GMR.PF, da^Scer\UAS.cGa, Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, wg^{wg.3'UTR.Scer\UAS} (Cadigan et al., 2002)
NOT Suppressor of
Statement Reference l(1)sc^Scer\UAS.cHa/da^Scer\UAS.cGa, Scer\GAL4^da.G32 is a non-suppressor of muscle founder cell phenotype of N^{int.SH.Scer\UAS}, Scer\GAL4^da.G32 (Giebel, 1999) l(1)sc^Scer\UAS.cHa/da^Scer\UAS.cGa, Scer\GAL4^da.G32 is a non-suppressor of presumptive embryonic/larval nervous system phenotype of Dl::N^{ΔECN.Scer\UAS}, Scer\GAL4^da.G32 (Giebel et al., 1997) Scer\GAL4^tub.PU/da^Scer\UAS.cGa is a non-suppressor of adult thorax & microchaeta \| somatic clone phenotype of sens^E2 (Jafar-Nejad et al., 2006) Scer\GAL4^tub.PU/da^Scer\UAS.cGa is a non-suppressor of eo neuron \| supernumerary \| somatic clone phenotype of sens^E2 (Jafar-Nejad et al., 2006) Scer\GAL4^tub.PU/da^Scer\UAS.cGa is a non-suppressor of tormogen cell \| somatic clone phenotype of sens^E2 (Jafar-Nejad et al., 2006) Scer\GAL4^tub.PU/da^Scer\UAS.cGa is a non-suppressor of trichogen cell \| somatic clone phenotype of sens^E2 (Jafar-Nejad et al., 2006)
Other
Statement Reference Scer\GAL4^da.G32, da^Scer\UAS.cGa, l(1)sc^Scer\UAS.cHa has embryonic/larval somatic muscle phenotype (Giebel, 1999, Giebel, 1999) Scer\GAL4^da.G32, da^Scer\UAS.cGa, l(1)sc^Scer\UAS.cHa has Malpighian tubule tip cell phenotype (Hoch and Jackle, 1998, Hoch and Jackle, 1998) Scer\GAL4^da.G32, da^Scer\UAS.cGa, l(1)sc^Scer\UAS.cHa has peripheral nervous system phenotype (Giebel, 1999, Giebel, 1999) Scer\GAL4^Eq1, da^Scer\UAS.cGa, sc^10-1, sens^Scer\UAS.cNa has adult thoracic sensillum phenotype (Jafar-Nejad et al., 2006, Jafar-Nejad et al., 2006, Jafar-Nejad et al., 2006) Scer\GAL4^Eq1, da^Scer\UAS.cGa, sc^10-1 has adult thoracic sensillum phenotype (Jafar-Nejad et al., 2006) Scer\GAL4^sca-109-68, da^Scer\UAS.cGa, sens^Scer\UAS.cNa has wing vein L3 & chaeta \| ectopic phenotype (Jafar-Nejad et al., 2006) Scer\GAL4^sca-537.4, amos^Scer\UAS.cHa, da^Scer\UAS.cGa has embryonic/larval neuron \| ectopic phenotype (Huang et al., 2000, Huang et al., 2000) Scer\GAL4^twi.2PE, da^Scer\UAS.cGa, tin^Scer\UAS.cRa has corpus cardiacum primordium phenotype (Park et al., 2011) Scer\GAL4^twi.PB, da^Scer\UAS.cGa, twi¹ has embryonic/larval somatic muscle phenotype (Castanon et al., 2001, Castanon et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Coexpression of both da^Scer\UAS.cGa and sens^Scer\UAS.cNa, under the control of Scer\GAL4^sca-109-68, generates 63.5 sensory organs, composed of both bristles and campaniform sensilla, along the third wing vein. This is an enhancement of the phenotype seen when either transgene is expressed alone and is greater than an additive effect. Expression of da^Scer\UAS.cGa in the thorax, under the control of Scer\GAL4^Eq1, leads to the generation of numerous bristles on the thorax of sc^10-1 mutants. These mutants have no thoracic bristles without the transgene expression. Coexpression of da^Scer\UAS.cGa and sens^Scer\UAS.cNa in the thorax, under the control of Scer\GAL4^Eq1, leads to the generation of multiple bristles on the thorax of sc^10-1 mutants; these bristles are present at a much greater density than those on wild-type thoraces or indeed the density produced by expressing either da^Scer\UAS.cGa or sens^Scer\UAS.cNa alone. Overexpression of da^Scer\UAS.cGa, under the control of Scer\GAL4^Eq1, results in the formation of bristles in Df(1)260-1 clones, which are usually devoid of bristles. (Jafar-Nejad et al., 2006) Co-expression of da^Scer\UAS.cGa and nvy^Scer\UAS.cWa, both under the control of Scer\GAL4^pnr-MD237, causes a bristle phenotype that is intermediate to the two phenotypes produced by single expression of either; flies have fewer macrochaetae on the thorax than wild type. Inhibition of nvy expression, achieved by expressing nvy^{dsRNA.Scer\UAS} under the control of Scer\GAL4^pnr-MD237, enhances the extra bristle phenotype of Scer\GAL4^pnr-MD237 > da^Scer\UAS.cGa flies. (Wildonger and Mann, 2005) Co-expression of da^Scer\UAS.cGa does not significantly alter the small eye phenotype caused by expression of wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF, but does fully suppress the loss of interommatidial bristles seen in these flies. Co-expression of da^Scer\UAS.cGa restores some progression of the morphogenetic furrow in animals expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of da^Scer\UAS.cGa significantly rescues progression of the morphogenetic furrow in animals expressing arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1. (Cadigan et al., 2002) When twi¹/+ is added to da^Scer\UAS.cGa, Scer\GAL4^twi.PB embryos, a dramatic suppression of somatic muscle fate. No increase in other mesodermal tissues is seen. (Castanon et al., 2001) When twi¹/+ is added to da^Scer\UAS.cGa, Scer\GAL4^twi.PB embryos, there is a dramatic suppression of somatic muscle fate. No increase in other mesodermal tissues is seen. (Castanon et al., 2001) Expression of both amos^Scer\UAS.cHa and da^Scer\UAS.cGa under the control of Scer\GAL4^sca-537.4 results in the induction of numerous elav-positive neurons. (Huang et al., 2000) Co-expression of l(1)sc^Scer\UAS.cHa and da^Scer\UAS.cGa driven by Scer\GAL4^da.G32 results in muscle and PNS pattern formation defects and in very rare cases, muscle duplication. The addition of l(1)sc^Scer\UAS.cHa and da^Scer\UAS.cGa does not rescue the mesoderm phenotype of N^{int.SH.Scer\UAS} driven by Scer\GAL4^da.G32. (Giebel, 1999) Some embryos coexpressing l(1)sc^Scer\UAS.cHa and da^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 develop multiple Malpighian tubule tip cells. (Hoch and Jackle, 1998) Coexpression of da^Scer\UAS.cGa with l(1)sc^Scer\UAS.cHa causes a similar phenotype to Scer\GAL4^da.G32-mediated expression of da^Scer\UAS.cGa. Scer\GAL4^da.G32-mediated expression of Dl::N^{ΔECN.Scer\UAS} generates aneural embryos. Simultaneous expression of da^Scer\UAS.cGa and l(1)sc^Scer\UAS.cHa does not rescue the phenotype. (Giebel et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^da.G32/da^Scer\UAS.cGa rescues da¹⁰ (Giebel et al., 1997) Scer\GAL4^da.G32/da^Scer\UAS.cGa rescues da^IIP106 (Giebel et al., 1997) Scer\GAL4^da.G32/da^Scer\UAS.cGa rescues da^KE38 (Giebel et al., 1997)
Comments	Scer\GAL4^da.G32-mediated expression. (Giebel et al., 1997)
Stocks ( 1 )
Bloomington	37291 w^*; P{UAS-da.G}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	da^Scer\UAS.cGa da^UAS.cGa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Giebel (Giebel et al., 1997)
Secondary FlyBase IDs

References ( 12 )
Research paper	Egoz-Matia et al., 2011, Dev. Biol. 351(1): 99--109 Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression. [FBrf0213026] Park et al., 2011, PLoS Genet. 7(8): e1002241 Specification of Drosophila corpora cardiaca neuroendocrine cells from mesoderm is regulated by Notch signaling. [FBrf0215231] Salzer et al., 2010, Genetics 184(1): 185--197 The retinal determination gene eyes absent is regulated by the EGF receptor pathway throughout development in Drosophila. [FBrf0209690] Bhattacharya and Baker, 2009, Dev. Biol. 327(2): 288--300 The HLH protein Extramacrochaetae is required for R7 cell and cone cell fates in the Drosophila eye. [FBrf0207062] Jafar-Nejad et al., 2006, Development 133(9): 1683--1692 Senseless and Daughterless confer neuronal identity to epithelial cells in the Drosophila wing margin. [FBrf0190299] Wildonger and Mann, 2005, Dev. Biol. 286(2): 507--520 Evidence that nervy, the Drosophila homolog of ETO/MTG8, promotes mechanosensory organ development by enhancing Notch signaling. [FBrf0190182] Cadigan et al., 2002, Development 129(14): 3393--3402 Wingless blocks bristle formation and morphogenetic furrow progression in the eye through repression of Daughterless. [FBrf0151912] Castanon et al., 2001, Development 128(16): 3145--3159 Dimerization partners determine the activity of the Twist bHLH protein during Drosophila mesoderm development. [FBrf0138569] Huang et al., 2000, Neuron 25(1): 57--67 The proneural gene amos promotes multiple dendritic neuron formation in the Drosophila peripheral nervous system. [FBrf0126761] Giebel, 1999, Mech. Dev. 86(1-2): 137--145 The Notch signaling pathway is required to specify muscle progenitor cells in Drosophila. [FBrf0111372] Hoch and Jackle, 1998, EMBO J. 17(19): 5766--5775 Kruppel acts as a developmental switch gene that mediates Notch signalling-dependent tip cell differentiation in the excretory organs of Drosophila. [FBrf0104911] Giebel et al., 1997, Mech. Dev. 63(1): 75--87 Lethal of scute requires overexpression of daughterless to elicit ectopic neuronal development during embryogenesis in Drosophila. [FBrf0093461]

Allele Dmel\daScer\UAS.cGa

Allele Dmel\da^Scer\UAS.cGa