|Name||Saccharomyces cerevisiae UAS construct a of Giebel||FlyBase ID||FBal0061468|
|Feature type||allele||Associated gene||Dmel\da|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
The number of gl-positive corpora cardiaca precursor cells is normal in stage 12 embryos expressing da[Scer\UAS.cGa] under the control of Scer\GAL4[twi.2PE].
R7 development is not affected by da[Scer\UAS.cGa] expression, as judged from normal run and pros expression.
Overexpression of daScer\UAS.cGa, under the control of Scer\GAL4sca-109-68, generates 17.1 extra sensory organs along the third wing vein compared to wild type wings.
Expression of daScer\UAS.cGa, under the control of Scer\GAL4pnr-MD237, results in the presence of extra macrochaetae on the adult thorax.
Over expression of daScer\UAS.cGa, when driven by Scer\GAL4twi.PB has little or no effect on mesoderm development.
Expression of daScer\UAS.cGa under the control of Scer\GAL4sca-537.4 has only a minor effect on the number of elav-positive neurons.
Scer\GAL4da.G32-mediated embryonic lethality is due to extensive neural defects. Two copies of daScer\UAS.cGa cause a stronger phenotype: embryos exhibit incomplete germ band retraction, head involution and dorsal closure, defects in connectives and commissures are more frequent.
|Phenotype Manifest In|
Scer\GAL4pnr-MD237, daScer\UAS.cGa has macrochaeta | supernumerary phenotype, enhanceable by nvydsRNA.Scer\UAS
Scer\GAL4Eq1, daScer\UAS.cGa has adult thoracic sensillum phenotype, suppressible | partially by Df(1)260-1
daScer\UAS.cGa is a suppressor | partially of adult thorax | dorsal & mechanosensory sensory organ phenotype of Scer\GAL4pnr-MD237, nvyScer\UAS.cWa
Scer\GAL4dpp.blk1/daScer\UAS.cGa is a suppressor | partially of morphogenetic furrow phenotype of Scer\GAL4dpp.blk1, wgwg.3'UTR.Scer\UAS
Scer\GAL4dpp.blk1/daScer\UAS.cGa is a suppressor of morphogenetic furrow phenotype of Scer\GAL4dpp.blk1, armS10.Scer\UAS.T:Hsap\MYC
Scer\GAL4GMR.PF, daScer\UAS.cGa, Scer\GAL4GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, wgwg.3'UTR.Scer\UAS
|NOT Suppressor of|
l(1)scScer\UAS.cHa/daScer\UAS.cGa, Scer\GAL4da.G32 is a non-suppressor of muscle founder cell phenotype of Nint.SH.Scer\UAS, Scer\GAL4da.G32
l(1)scScer\UAS.cHa/daScer\UAS.cGa, Scer\GAL4da.G32 is a non-suppressor of presumptive embryonic/larval nervous system phenotype of Dl::NΔECN.Scer\UAS, Scer\GAL4da.G32
Scer\GAL4tub.PU/daScer\UAS.cGa is a non-suppressor of adult thorax & microchaeta | somatic clone phenotype of sensE2
Scer\GAL4tub.PU/daScer\UAS.cGa is a non-suppressor of eo neuron | supernumerary | somatic clone phenotype of sensE2
Scer\GAL4tub.PU/daScer\UAS.cGa is a non-suppressor of tormogen cell | somatic clone phenotype of sensE2
Scer\GAL4sca-537.4, amosScer\UAS.cHa, daScer\UAS.cGa has embryonic/larval neuron | ectopic phenotype
Coexpression of both daScer\UAS.cGa and sensScer\UAS.cNa, under the control of Scer\GAL4sca-109-68, generates 63.5 sensory organs, composed of both bristles and campaniform sensilla, along the third wing vein. This is an enhancement of the phenotype seen when either transgene is expressed alone and is greater than an additive effect. Expression of daScer\UAS.cGa in the thorax, under the control of Scer\GAL4Eq1, leads to the generation of numerous bristles on the thorax of sc10-1 mutants. These mutants have no thoracic bristles without the transgene expression. Coexpression of daScer\UAS.cGa and sensScer\UAS.cNa in the thorax, under the control of Scer\GAL4Eq1, leads to the generation of multiple bristles on the thorax of sc10-1 mutants; these bristles are present at a much greater density than those on wild-type thoraces or indeed the density produced by expressing either daScer\UAS.cGa or sensScer\UAS.cNa alone. Overexpression of daScer\UAS.cGa, under the control of Scer\GAL4Eq1, results in the formation of bristles in Df(1)260-1 clones, which are usually devoid of bristles.
Co-expression of daScer\UAS.cGa and nvyScer\UAS.cWa, both under the control of Scer\GAL4pnr-MD237, causes a bristle phenotype that is intermediate to the two phenotypes produced by single expression of either; flies have fewer macrochaetae on the thorax than wild type. Inhibition of nvy expression, achieved by expressing nvydsRNA.Scer\UAS under the control of Scer\GAL4pnr-MD237, enhances the extra bristle phenotype of Scer\GAL4pnr-MD237 > daScer\UAS.cGa flies.
Co-expression of daScer\UAS.cGa does not significantly alter the small eye phenotype caused by expression of wgwg.3'UTR.Scer\UAS under the control of Scer\GAL4GMR.PF, but does fully suppress the loss of interommatidial bristles seen in these flies. Co-expression of daScer\UAS.cGa restores some progression of the morphogenetic furrow in animals expressing wgwg.3'UTR.Scer\UAS under the control of Scer\GAL4dpp.blk1. Co-expression of daScer\UAS.cGa significantly rescues progression of the morphogenetic furrow in animals expressing armS10.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4dpp.blk1.
When twi1/+ is added to daScer\UAS.cGa, Scer\GAL4twi.PB embryos, a dramatic suppression of somatic muscle fate. No increase in other mesodermal tissues is seen.
When twi1/+ is added to daScer\UAS.cGa, Scer\GAL4twi.PB embryos, there is a dramatic suppression of somatic muscle fate. No increase in other mesodermal tissues is seen.
Expression of both amosScer\UAS.cHa and daScer\UAS.cGa under the control of Scer\GAL4sca-537.4 results in the induction of numerous elav-positive neurons.
Co-expression of l(1)scScer\UAS.cHa and daScer\UAS.cGa driven by Scer\GAL4da.G32 results in muscle and PNS pattern formation defects and in very rare cases, muscle duplication. The addition of l(1)scScer\UAS.cHa and daScer\UAS.cGa does not rescue the mesoderm phenotype of Nint.SH.Scer\UAS driven by Scer\GAL4da.G32.
Some embryos coexpressing l(1)scScer\UAS.cHa and daScer\UAS.cGa under the control of Scer\GAL4da.G32 develop multiple Malpighian tubule tip cells.
Coexpression of daScer\UAS.cGa with l(1)scScer\UAS.cHa causes a similar phenotype to Scer\GAL4da.G32-mediated expression of daScer\UAS.cGa. Scer\GAL4da.G32-mediated expression of Dl::NΔECN.Scer\UAS generates aneural embryos. Simultaneous expression of daScer\UAS.cGa and l(1)scScer\UAS.cHa does not rescue the phenotype.
|Complementation & Rescue Data|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Giebel
|Secondary FlyBase IDs|
|References ( 12 )|