Open Close
General Information
Symbol
Dmel\hidUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zhou
FlyBase ID
FBal0061531
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hid, P[UAS-hid], UAShid
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a hid cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing hidUAS.cZa under the control of either Scer\GAL4Gr66a.PD or Scer\GAL4Ir7a.PC show decreased gustatory avoidance to food laced with acetic acid (ie. no avoidance to 5% acetic acid), as compared to controls; GRNs enervating their S6 and S10 sensilla show greatly reduced responses (frequency of spikes) to acetic acid, as compared to controls.

Ablation of Crz neurons by expressing WScer\UAS.cZa under the control of Scer\GAL4Crz.PC results in flies that show a significantly delayed recovery from ethanol-induced sedation. Whole cell ALDH activity is reduced compared to controls but ADH activity is unaffected.

In equinox or light-dark 12:12 conditions, the evening activity bout of npf ablated flies (WScer\UAS.cZa under the control of Scer\GAL4npf.1) is shaped differently in comparison to control flies. These flies show a linear increase in activity from the middle to the end of the light-phase, following by a reduction in amplitude in comparison to control flies, resulting in an activity plateau before lights-off, indicating that npf-ablated flies reach their maximum activity level earlier than control flies. In LD 16:08 (i.e. 16hrs of light and 8hrs of dark) conditions, npf-ablated flies reach their maximum activity in the evening earlier than control flies and the activity plateau is even more pronounced.

The evening activity of flies expressing WScer\UAS.cZa under the control of Scer\GAL4npf.1 already reaches its peak 2 hours before lights-off and remains at the same level until lights-off.

Flies expressing WScer\UAS.cZa under the control of Scer\GAL4npf.1 in non-Clk neurons (i.e. through expression of Scer\GAL80cry.PS which blocks expression in Clk neurons) exhibit the same gradual increase in activity before lights-off in LD 12:12 and 16:08 as found in control flies, with the flies exhibiting a clear peak of evening activity rather than a flat plateau, as in WScer\UAS.cZa Scer\GAL4npf.1 flies, in the longer photoperiod. The activity of Scer\GAL80cry.PS-rescued flies still increases significantly within two hours before lights-off, indicating that the activity level in the late evening is not reduced, as in WScer\UAS.cZa Scer\GAL4npf.1 flies.

Flies expressing WScer\UAS.cZa under the control of Scer\GAL4npf.1 in non-Clk neurons (i.e. through expression of Scer\GAL80Pdf.PS which blocks expression in Pdf neurons) show a small activity plateau in the very late evening compared to controls in LD 12:12 conditions. However, this effect is not as pronounced as in WScer\UAS.cZa Scer\GAL4npf.1 flies, as there is still a significant increase in activity within 2 hours before lights-off in Scer\GAL80Pdf.PS-rescued flies. In LD 16:08 the evening activity of Scer\GAL80Pdf.PS-rescued flies becomes clearly comparable to npf-ablated flies, which show the same activity plateau in this photoperiod. The relative amplitude of the evening activity in Scer\GAL80Pdf.PS-rescued flies is reduced.

Pdf-expressing neuron ablation, through expression of WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf clearly advances the phase of the evening activity in LD 12:12 conditions, compared to control flies. This becomes more obvious in LD 16:08. The relative amplitude of the evening activity is not reduced.

Expression of WScer\UAS.cZa in Pdf- and npf-expressing neurons (under the control of Scer\GAL4P2.4.Pdf and Scer\GAL4npf.1) results in all lateral clock neurons, except for new npf-negative LN[[d]]s and the LPNs are ablated. In LD 12:12 , the evening activity of these flies even decreases before lights-off after reaching a peak, indicating that the phase of the evening activity is even more advanced compared to Pdf-ablated flies. In LD 16:08 the evening activity peak of npf/Pdf-ablated flies occurs clearly earlier than the peak of Pdf-ablated flies. In addition, the relative amplitude of the evening activity in npf/Pdf-ablated flies is reduced in comparison to Pdf-ablated flies.

The majority of npf-ablated flies (through expression of WScer\UAS.cZa under the control of Scer\GAL4npf.1) show clear free-running rhythms in DD, but chi[2]-periodogram analysis reveals that they have a significantly prolonged free-running period compared to control flies. The free-running period of Scer\GAL80cry.PS-rescued flies is significantly shorter than the period of npf-ablated flies and even shorter than in controls. In contrast, the Scer\GAL80Pdf.PS-mediated rescue of npf-positive I-LN[[v]]s does not have the same effect. The free-running period of Scer\GAL80Pdf.PS-rescued flies is not significantly different from the period of npf-ablated flies or controls.

Ablation of Pdf neurons through expression of WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf results in the loss of the morning peak of locomotor activity. However, these flies are still able to increase their preferred temperature to similar levels as in control flies during LD.

Ablation of cry neurons through expression of WScer\UAS.cZa under the control of Scer\GAL4cry.PE results in the loss of both the morning and evening peaks of locomotor activity. However, these flies are still able to increase their preferred temperature to similar levels as in control flies during LD.

Ectopic expression of WScer\UAS.cZa in the hemocytes under the control of Scer\GAL4Hml.PG results in the near complete ablation of hemocytes by the 1st instar larval stage and generates 3rd instar larvae that are completely devoid of hemocytes.

Expression of WScer\UAS.cZa under the control of Scer\GAL4Tdc2.PC eliminates the octopaminergic neurons in early third instar larvae, resulting in substantially reduced locomotor speed and a reduction in the starvation response (after 2 hours of food deprivation the number of synaptopods is significantly increased).

Expression of WScer\UAS.cZa in octopaminergic neurons, under the control of Scer\GAL4Tdc2.PC, results in the elimination of type II boutons. The absence of type II innervation leads to a substantial reduction in the number of type I boutons.

Expression of hidScer\UAS.cZa under the control of Scer\GAL4btl.PU causes gaps in the dorsal trunk as well as disintegration of some of the trachea. When hidScer\UAS.cZa is expressed at a higher temperature there is also loss of one of more of the salivary ducts.

When daylight-moonlight and temperature cycles are in phase, Scer\GAL4P2.4.Pdf/WScer\UAS.cZa flies (that retain all clock neurons except the s-LN[[v]] and l-LN[[v]] cells) display a clear evening peak with a slightly earlier phase than wild-type flies. After the shift of daylight-moonlight cycles, the evening peak follows, maintaining its slightly earlier phase as compared to wild-type flies.

Scer\GAL4cry.PE/WScer\UAS.cZa flies, that lack all cry-positive neurons, display clearly 2 activity peaks, in the late morning and in the evening, when daylight-moonlight and temperature cycles are in phase. Thus, the activity pattern of Scer\GAL4cry.PE/WScer\UAS.cZa flies is quite normal, although there are some differences relative to wild-type flies. After the shift of the daylight-moonlight cycles, the morning peak disappears, but the evening peak maintains approximately it's previous phase, slightly broadened and slightly delayed.

Scer\GAL4desat1.PB, Scer\GAL80ts.αTub84B/WScer\UAS.cZa flies lack oenocytes.

Wild-type females are less receptive to oenocyte-less Scer\GAL4desat1.PB, Scer\GAL80ts.αTub84B/WScer\UAS.cZa males than control males, with the oenocyte-less males taking almost four times as long to achieve mating. Scer\GAL4desat1.PB, Scer\GAL80ts.αTub84B/WScer\UAS.cZa oenocyte-less males elicited courtship and copulation attempts from both wild-type males and other oenocyte-less males, indicating that oenocyte-less males are perceived as females, even though male characteristics are present. Wild-type males exhibit normal courtship behaviour towards Scer\GAL4desat1.PB, Scer\GAL80ts.αTub84B/WScer\UAS.cZa oenocyte-less females. However, mating latency is significantly shorter, and when given a choice between oenocyte-less and control female, wild-type males preferred oenocyte-less females. Thus, any Scer\GAL4desat1.PB, Scer\GAL80ts.αTub84B/WScer\UAS.cZa oenocyte-less fly, irrespective of its development as female or male, seems to sexually hyperstimulate males.

Under light:dark (LD) conditions, flies expressing WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf show increases in sleep during the late night. At the time when wild-type flies are waking up in advance of lights-on, the mutant flies fail to wake up. In addition, the mutant flies show increases in sleep during the early day (ZT 1-2) and early night (ZT 13-14) as well as reduced sleep consolidation at night.

During the first day of constant darkness (DD) conditions, flies expressing WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf flies show a significant increase in total sleep during the subjective day compared to control flies.

Expression of WScer\UAS.cZa using Scer\GAL4Gr66a.PD abolishes the L-canavanine-induced proboscis retraction seen in wild type flies.

Expression of WScer\UAS.cZa under the control of Scer\GAL4R15W.elav results in an erect wing phenotype at 18, 25, and 31oC.

Expression of WScer\UAS.cZa under the control of Scer\GAL4elav.PLu results in death at 18, 25 or 31oC.

Expression of WScer\UAS.cZa under the control of Scer\GAL4K23P.elav results in death at 18oC and abnormal eye defects at 25 and 31oC.

Expression of WScer\UAS.cZa under the control of Scer\GAL4elav.PLu in a Scer\GAL80αTub84B.PL background results in death at either 18oC or 31oC.

Expression of WScer\UAS.cZa under the control of Scer\GAL4R15W.elav in a Scer\GAL80αTub84B.PL background at 31oC reveals a normal eye phenotype, whereas those at 18oC do not survive.

Expression of WScer\UAS.cZa under the control of Scer\GAL4GMR.PM in a Scer\GAL80αTub84B.PL background results in abnormal eyes at 18oC and extensive eye defects at 31oC.

Expression of WScer\UAS.cZa under the control of either Scer\GAL4K23P.GMR or Scer\GAL4R15W.GMR results in abnormal eyes at 18oC and close to normal eyes at 31oC.

Expression of WScer\UAS.cZa under the control of Scer\GAL4moody.SPG from the end of larval development onwards (using the temperature sensitive Scer\GAL80ts.αTub84B allele to repress expression before this stage) results in ablation of the carpet glial cells. Glial cells originating from the optic stalk migrate further onto the eye imaginal disc epithelium than normal.

Expression of WScer\UAS.cZa under the control of Scer\GAL4ppk.1.9 results in the collapse of the nuclear compartment and fragmentation of the axon, reminiscent of other da neurons that die during morphogenesis.

Ablation of Rh5-specific photoreceptors by the targeted expression of WScer\UAS.cZa causes a severe reduction in response to light. Head-swinging behaviour, in response to light stimulation is severely reduced in these mutants.

Targeted ablation of Rh6-specific photoreceptors by the targeted expression of WScer\UAS.cZa does not affect larval response to light or head swinging behaviour (in response to light stimulation).

Ablation of Rh6 neurons through expression of WScer\UAS.cZa under the control of Scer\GAL4Rh5.PT reduces the number of photoreceptor axons. This is not the case when Rh5 neurons are ablated (through expression of WScer\UAS.cZa under the control of Scer\GAL4Rh6.PD). The projection pattern of the remaining axons in either case is indistinguishable from wild-type.

Severe lethality is seen when WScer\UAS.cZa is expressed using Scer\GAL4GMR.PU, presumably due to leaky Scer\GAL4 expression. Animals also show a small-eye phenotype.

Expression of WScer\UAS.cZa using Scer\GAL4Rh3.PP does not cause any lethality. However, R7 photoreceptor neurons are absent in some ommatidia, and this phenotype worsens significantly with age.

Expression of WScer\UAS.cZa in all Rh5 photoreceptors, driven by Scer\GAL4Rh5.PT, causes ablation of Rh5 photoreceptors. This has no obvious effect on 5-HT arborization. However, expression of WScer\UAS.cZa in all Rh6 photoreceptors, driven by Scer\GAL4Rh6.PD, leads to significant reduction in 5-HT arborization.

Animals expressing WScer\UAS.cZa under the control of Scer\GAL4TrpA1.PR have a reduced number of TrpA1-expressing brain neurons, while the corpus cardiacum appears unaffected. Third instar larvae expressing WScer\UAS.cZa under the control of Scer\GAL4TrpA1.PR are partially, but significantly compromised in themotactic behaviour in a thermal preference assay, but show normal withdrawal from high temperature nocioceptive stimulus.

When WScer\UAS.cZa is driven by Scer\GAL4cry.PE, a very strong phenotype is seen in the pattern of mutant flies' LD circadian locomotor activity - no anticipation of either lights on or light off is seen. In DD conditions locomotor activity is also completely arrhythmic. When WScer\UAS.cZa is driven by Scer\GAL4cry.PE, and repressed by Scer\GAL80Pdf.PS, exhibit a suppressed evening activity before lights of in LD conditions. In DD conditions these flies exhibit a robust and sustained rhythm with a wild-type period. However they have a unimodal, apparently morning only activity pattern. The timing of the morning locomotor activity increase is indistinguishable from that of wild-type but flies fail to suppress strongly the activity after subjective morning. This prolonged activity bout delays the calculated phase by more than 2 hours relative to the phase of wild-type morning activity. These flies also exhibit an absence of a robust DD evening activity bout. Although these flies are only weakly rhythmic during the first few days of DD, the absence of a morning peak is evident.

When WScer\UAS.cZa is driven by Scer\GAL4P2.4.Pdf mutant animals exhibit a reduced sensitivity to cocaine.

When WScer\UAS.cZa is driven by Scer\GAL4P2.4.Pdf, the lateral neurons are ablated. These animals are arrhythmic for rest:activity behavior as adults. Eclosion gating appears to be present during the first two days in constant darkness, rhythms do not persist.

Most flies expressing WScer\UAS.cZa under the control of Scer\GAL4GMR.PF die shortly after pupariation. Flies expressing WScer\UAS.cZa under the control of Scer\GAL4rdgC.PK survive well to adulthood and show no defects in walking behaviour.

Expression of WScer\UAS.cZa under the control of Scer\GAL4GMR.in.Switch.PR in animals exposed to RU486 results in an eye phenotype ranging from substantial tissue loss to complete ablation. Expression of WScer\UAS.cZa under the control of Scer\GAL4GMR.in.Switch.PR in the absence of RU486 does not result in eye ablation, but the eyes have reduced pigmentation.

WScer\UAS.cZa when driven by Scer\GAL4eya.PB causes a reduced eye phenotype. These flies no other phenotype.

The locomotor activity of flies expressing WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf is not entirely normal. The flies are entrained during 12 hour light:12 hour dark (LD) cycles, but the evening locomotor phase is advanced by at least 0.5 hours compared to wild-type flies. 17% of flies are rhythmic for the entire duration of a 9 day period under constant darkness. The proportion of rhythmic individuals decreases over time under constant darkness. Flies that are persistently rhythmic tend to have short periods.

Expression of WScer\UAS.cZa under the control of Scer\GAL4Eh.2.4 results in semilethality, with most animals dying during the larval period, crawling out of the food before dying. Most of the larvae have tracheal abnormalities; 80% have dorsal tracheae filled with fluid. Old tracheae are seen inside new ones. 34% of animals expressing WScer\UAS.cZa under the control of Scer\GAL4Eh.2.4 eclose as fertile, viable adults. Eh expressing neurons die during the first or second larval stages in animals expressing WScer\UAS.cZa under the control of Scer\GAL4Eh.2.4. The neurons appear to disintegrate progressively.

Scer\GAL4Gp150-52A-mediated expression of rprScer\UAS.cZa alone is not sufficient to induce substantial apoptosis in the embryonic CNS midline cells. The midline cells survive and are capable of carrying out normal axon guidance functions (axon scaffold organisation is normal).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Other
Statement
Reference

Scer\GAL4nrv2.PS, hidUAS.cZa, rprUAS.cZa has lethal | temperature conditional phenotype

Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Other
Statement
Reference

Scer\GAL4nrv2.PS, hidUAS.cZa, rprUAS.cZa has glial cell | temperature conditional phenotype

Additional Comments
Genetic Interactions
Statement
Reference

No wing phenotypes are seen when hidScer\UAS.cZa is expressed under the control of Scer\GAL4azot-GAL4 in azotKO.GAL4/+ mutant flies, either with or without exposure to radiation.

Co-expression of WScer\UAS.cZa and rprScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 is lethal, but is viable when Scer\GAL80αTub84B.PL is expressed until the third instar larval stage. Robust cell death is seen in the wing disc 12 hours after Scer\GAL4 de-repression.

When larvae expressing WScer\UAS.cZa and rprScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 are irradiated (and expression is repressed until 12 hours prior to irradiation using Scer\GAL80αTub84B.PL), less cell death is seen in the wing disc in comparison with irradiated controls. A heterozygous Df(3L)banΔ1 background has no effect on the amount of cell death, but when de-repression occurs 6 hours prior to irradiation (which results in a less dramatic reduction in cell death compared to 12 hours), heterozygous Df(3L)banΔ1 partially suppresses the level of protection seen when WScer\UAS.cZa and rprScer\UAS.cZa are expressed alone.

When wing discs containing WScer\UAS.cZa and rprScer\UAS.cZa expressing clones (repressed during early development using Scer\GAL80αTub84B.PL)) are irradiated, less cell death is seen outside of the clones in comparison with irradiated controls.

Flies co-expressing rprScer\UAS.cUa and WScer\UAS.cZa under the control of Scer\GAL4Crz.PU show a significant reduction in ethanol sedation sensitivity compared to wild type.

A Ras85DR68Q background can partially suppress the hemocyte death seen upon expression of WScer\UAS.cZa under the control of Scer\GAL4Hml.PG. Hemocytes are clearly visible anteriorly in the lymph glands of 3rd instar larvae. Circulating hemocytes, however, appear to remain susceptible to W-induced cell death and are missing, even in the presence of two copies of Ras85DR68Q.

Co-expression of WScer\UAS.cZa and rprScer\UAS.cZa in the posterior signalling center under the control of Scer\GAL4Antp-10 has no effect on lamellocyte differentiation.

Ablation of male oenocytes (and associated cuticular hydrocarbons) through expression of WScer\UAS.cZa, and Avic\GFPStinger.Scer\UAS.T:nls-tra under the control of Scer\GAL4desat1.PB and Scer\GAL80ts.αTub84B at 18[o]C during development and 25[o]C at the adult stage results in substantially reduced levels of male-male aggression compared with pairs of control males, in addition to higher levels of male-male courtship. However, aggression between such oenocyte-eliminated males is not completely eliminated. Control flies show markedly lower levels of male-male aggression towards oenocyte-eliminated males than control males, as measured by the occurrence of lunges. Wing threat display is unaffected by the elimination of male cuticular hydrocarbons. Control males show higher levels of courtship towards oenocyte-eliminated than toward control target males, as measured by the occurrence of unilateral wing extensions and circling episodes. Restoring cuticular hydrocarbons to oenocyte-eliminated males through passive transfer from control flies rescues both the decrease male-male aggression and increase male-male courtship.

Passive addition of synthetic (z)-7-tricosene to oenocyte-eliminated male flies (through expression of WScer\UAS.cZa, and Avic\GFPStinger.Scer\UAS.T:nls-tra under the control of Scer\GAL4desat1.PB and Scer\GAL80ts.αTub84B at 18[o]C during development and 25[o]C at the adult stage) restores normal levels of male-male aggression, and suppresses the increased levelsl of male-male courtship, by wild-type males.

Males co-expressing WScer\UAS.cZa and rprScer\UAS.C under the control of Scer\GAL4CheB42a.ppk25 show courtship latency (time from female introduction until the male shows any courtship related behaviours) similar to that of parental controls.

Males co-expressing WScer\UAS.cZa and rprScer\UAS.C under the control of Scer\GAL4CheB42a.ppk25 show a mild change in courtship index (the proportion of time a male spends courting once courting started) which is not statistically significant compared to controls.

Co-expression of WScer\UAS.cZa, grimScer\UAS.cOCa and rprScer\UAS.cZa under the control of Scer\GAL4ple.PF results in the volume of DA neurites being significantly diminished compared to controls.

Scer\GAL4Hml.PG-, Scer\GAL4Pxn.PS- or Scer\GAL4srp.D.cCa-mediated expression of rprScer\UAS.cZa and WScer\UAS.cZa ablates larval hemocytes.

Scer\GAL4srp.D.cCa-mediated expression of rprScer\UAS.cZa and WScer\UAS.cZa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.

Expression of both rprScer\UAS.cZa and WScer\UAS.cZa in the cortex and neuropile glia from late embryonic stage onwards, under the control of Scer\GAL4nrv2.PS at 18[o]C and switching to a high temperature (25[o]C) shortly after hatching allows most animals to develop to late larval stages. In these larvae, glial cell death sets in around hatching and is more or less complete by the early 2nd instar. These larval brains exhibit a greatly increased number of intra-neuropilar tracheal branches. Six or more secondary branches split off the perineuropilar plexus at variable positions and penetrate the neuropile. Larvae lacking cortex and neuropile glia (but retaining surface glia) show severe behavioural deficits, characterised by reduced peristaltic movement and feeding, and a constant 'tremor' of the pharyngeal and body wall musculature.

Males co-expressing rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL4IFa.PT show no difference in male-female courtship behaviour compared to control males when naive males are allowed to court virgin wild-type females. However, the mutant males show significant male-male courtship behaviour when naive males are allowed to court males.

Females co-expressing rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL4IFa.PT copulate more rapidly with male flies than control females.

Animals co-expressing rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf are arrhythmic for locomotor activity under constant light conditions at a constant temperature of 25[o]C, but become rhythmic almost immediately after being exposed to a temperature cycle of 12 hours at 25[o]C and 12 hours at 30[o]C. There is a peak at the beginning of the cryophase and a smaller peak at the early thermophase. The flies show a dispersed anticipatory activity starting around the middle of the thermophase, although transient cycles are not clear.

The electroantennogram (EAG) rhythm in response to ethyl acetate (measured on the second day of constant darkness conditions) in flies co-expressing rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf is similar to that of wild-type flies.

Third instar larvae co-expressing both rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL41118 have no obvious defects in the Bolwig nerve axonal projections although the lateral neurons are completely missing.

Co-expression of WScer\UAS.cZa and rprScer\UAS.cZa (when driven by Scer\GAL4Gp150-52A) results in ectopic midline cell death. The addition of thScer\UAS.T:Ivir\HA1 suppresses this phenotype, sometimes to wild-type.

The locomotor activity defects of flies expressing WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf are partially suppressed by coexpression of BacA\p35Scer\UAS.cHa.

When grimScer\UAS.cNa and rprScer\UAS.cZa are coexpressed, all the midline glia and some VUM neurons are eliminated. When grimScer\UAS.cNa and WScer\UAS.cZa are coexpressed, all the midline glia and VUM neurons are eliminated.

Mediated expression of both rprScer\UAS.cZa and WScer\UAS.cZa causes a striking loss of glial cells, significant defects in axon guidance scaffold are detected. VUM neurons exhibit greatly reduced sensitivity: cells are normal in position, number and morphology. Two copies of each construct completely eliminates all midline glia and dramatic loss of VUM neurons. Results indicate all midline glia are capable of undergoing cell death and suggests the midline neurons and glia may have different sensitivities to rpr and W expression. Simultaneous expression of BacA\p35Scer\UAS.cHa causes no ectopic cell death demonstrating that midline cell death induced by rpr and W require the functions or one or more caspases.

Xenogenetic Interactions
Statement
Reference

Coexpression of BacA\p35Scer\UAS.cHa rescues the loss of R7 neurons caused by Scer\GAL4Rh3.PP-mediated expression of WScer\UAS.cZa.

In WScer\UAS.cZa; BacA\p35Scer\UAS.cHa; Scer\GAL4en-e16E animals, increased BrdU incorporation is seen in the posterior compartment of the wing disc and in anterior compartment cells adjacent to the compartment boundary.

Increased BrdU incorporation in the posterior compartment of WScer\UAS.cZa; BacA\p35Scer\UAS.cHa; Scer\GAL4en-e16E is largely suppressed by panΔN.Scer\UAS.

WScer\UAS.cZa; BacA\p35Scer\UAS.cHa; Scer\GAL4αTub84B.PZ somatic clones in the wing disc cause significant overgrowth of this disc. This overgrowth in suppressed by NcC318G.Scer\UAS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
WScer\UAS.cZa
WUAS.cMa
WUAS.cZa
hidScer\UAS.cZa
hidUAS.cZa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Zhou
Secondary FlyBase IDs
  • FBal0083143
References (81)